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Since vitamin C is easily oxidized, storage and the cooking in air leads to the eventual oxidation of
vitamin C by oxygen in the atmosphere. This should succinctly summarize your data and any results
calculated from your data; tables and figures may be the best way to present this information. You
can download the paper by clicking the button above. However, the bilayer within the case of
niosomes is formed from non-ionic surface actives agents instead of phospholipids as seen just in
case of liposomes. Miao, Yi, Yuanyang Zhu, Wenzhu Zhao, Changshuai Jiao, Hongwei Mo, Xincheng
Zhang, Sheng Liu, and Hongwen Gao. After the independent-sample T-test between the standard
concentrations and the data obtained by the proposed method, the P-value was obtained. Ascorbic
acid functions as an anti-oxidant in living organisms protecting the body against the effect of
oxidative stress. It is difficult to identify how old fruit is due to the manner in which it is transported
from its country of origin. We use cookies on our website to ensure you get the best experience.
Repeat the procedure on two additional samples of standard ascorbic acid. Y means luminance, Z is
somewhat equal to blue, and X is a mix of cone response curves chosen to be orthogonal to
luminance and non-negative). Collecting data by observation, interviews, and documentation.
Determination of the concentration of vitamin C by using DCPIP test. With the designed PC
software, we could change the properties of the image by adjusting the camera parameters, such as
the hue, saturation, and white balance, and ensure that the whole measurement range had a good
discrimination by adjusting the camera parameters of exposure, brightness, and contrast. For non-
spectrophotometric methods are such as high-performance liquid chromatography (HPLC), titration,
enzymatic method and fluorometry. The titration method takes advantage of the ability of Iodine to
oxidise Vitamin C. When to measure: fasting state or 2 hours after taking a meal. When free radicals
are present in cells as high levels they are thought to have an effect on cardiovascular disease, high
blood pressure and chronic inflammatory diseases 3. International Journal of Translational Medicine
(IJTM). The goal of the present work was to measure VC content using a typical infrared camera.
Samples were chosen randomly in Tirana’s hypermarkets. This solution produced a red-coloured
complex with 85% H2SO4. The pipettes must be carefully cleaned by distilled water and rinsed with
the solution to be taken up. The homogenate was transferred to a 50 mL volumetric flask, and 20 mL
of 0.25 M acetic acid added to dilute to the scale of 50 mL, before we shook well to obtain a sample
solution. Since the ripening stage was not informed, the ascorbic acid concentration could have
varied and therefore affected the results of the experiment. Both groups were examined for
neuropathic symptoms and their medical history was recorded. Tropical Medicine and Infectious
Disease (TropicalMed). Perform at least three titrations, and repeat as necessary until at least three
runs are within 5% of each other. Other spelling, grammar and punctuation are all accurate. One-way
ANOVA was used to respectively analyze the seven sets of data measured based on L, grayscale, and
log-grayscale with the data of spectrophotometry.
No-pre scientific tests are done which would increase the candidates mark to identify any
confounding factors or biases and how the student would take this into account whilst setting out the
experiment. Each of the five levels of acetic acid added to drinking water (0.0, 0.1, 0.2, 0.3, and
0.4%) were given to five replicates for a period of 21 days. How to titrate vitamin C solution against
1 ml of DCPIP solution How to titrate juice against 1 ml of DCPIP solution Precaution: Drop size
must be constant. Iodine ethanol solution: 0.7 g of iodine was added into 30 mL of 95% ethanol,
with a FSH-2A adjustable high-speed homogenizer (Changzhou Guowang Instrument
Manufacturing Co., Ltd., China; ) to accelerate dissolving. Schematic presentation of the detection
of vitamin C (VC) with our proposed method. The stability of marketed fruit juices made up of
apple, grapes, lemon. Vitamin C is crucial for the development and maintenance of connective tissues
in humans. Next Article in Special Issue Assessment of Melting Kinetics of Sugar-Reduced Silver
Ear Mushroom Ice Cream under Various Additive Models. Therefore, following works should test
the feasibility of this method on a wider range of foods and complete the compensation algorithm
for different temperatures, which will provide more functionality to the method. The principle is that
suspended particles absorb and scatter the incident light, and the intensity of scattering and
absorption is positively proportional to the concentration of suspended particles. This study aims to
describe the application of micro-teaching practices to improve speaking skills in Arabic education
study programs during the pandemic. In a completely randomized design with 5 treatments and 5
replicates, 300 day-old commercial mail broiler chicks (ROSS) were divided into 25 groups, 12
chicks per group. The VC sample solutions were obtained. 2.4. The Principle of Measuring Vc Using
the Iodine-Turbidimetric Method The schematic presentation of the detection of VC is shown in
Figure 4. Perform at least three titrations, and repeat as necessary until at least three runs are within
5% of each other. Also available from the ChemLab web page are a Laboratory Report Template and
an example of a completed formal laboratory report. In the present work, it was aimed to determine
and compare the Vitamin C concentration in some fresh fruits available in the middle region of
Gujarat by using Titrimetric and UV spectrophotometric methods. Key words: Ascorbic acid,
iodometric titration, lemon, mang. The image acquired by the infrared camera was a single-channel
image. Chemistry Project for class 12 on a comparative study on different brands of. European
Journal of Investigation in Health, Psychology and Education (EJIHPE). It is thought that ascorbic
acid reacts with these free radicals preventing them from causing damage to cells. Prelaboratory
Assignment A 25.00-mL sample of orange dr in k with 2 g of KI and 25.00 mL of 0.0130 M KIO3
added was back-titrated with a 0.0311 M sodium thiosulfate solution. Total ascorbate was
determined by C18 reversed-phase LC with a phosphate eluent at pH 2.5, incorporating
dithiothreitol to maintain vitamin C in the reduced form, and UV detection at 254 nm. However, the
collected packed juices of the same fruits were simply unsealed and taken directly in a test tube for
analysis. Note that from the first issue of 2016, this journal uses article numbers instead of page
numbers. These observations may serve as pointers on selection of fruits that can be consumed to
match the requirement of vitamin C. The P-value—the level of significance—is the probability that
hypothesis. VC is necessary for preventing scurvy and is often called ascorbic acid in medicine. The
candidate could have used correct cm cubed terms, and could have improved their evaluation by
calculating percentage errors in the equipment used and there are no possible improvements to the
experiment suggested. You can download the paper by clicking the button above.
The comparison results controlled Q1 to realize the constant current driving of the LED. Vitamin C,
namely ascorbic acid, is vital for us: Electron donor with powerful antioxidant capacity Protect and
even re-generate other antioxidants: Vit.E, Vit.A, GSH. The adjusted R-Squared (Adj R-square),
relation expressions, limit of detection (LODs), limit of quantification (LOQs), and ranges are shown
in Table 1. The concentration of the detected substances in the solution can be deduced by measuring
the intensity of transmitted light. Potential future improvements regarding proper method validation
are indicated to conclude this review. However as the concentration of iodine and triiodide increases
the reaction between starch molecules is relatively strong and becomes less reversible. Be sure to
record the concentration of the standard ascorbic acid (it should be about 0.500 g of vitamin C per
1.000 L). Add 2 mL of the sulfuric acid mixture and about 25 mL of distilled water to the flask (
More Info ). Results were analyzed by two-sample t-test equal means. Solution can also be left
behind in flasks as it is transferred from one to the other. Parameters such as selectivity, linearity,
limit of quantification, and accuracy were studied by most authors. Objectives: Determination of
proteins using three spectrophotometric methods: Bicinchoninic acid (BCA, Smith) Method. As all of
the amounts measured in this experiment are similar (67.0%, 64.8% and 65.0%). It seems to suggest
that the error is a result of the experimental procedure for measuring the quantity of vitamin c and
not in the manner that it has been carried out. We were not however sure because we did not see it
being made. The following are some helpful hints for you to consider as you prepare your report. It is
thought that ascorbic acid reacts with these free radicals preventing them from causing damage to
cells. In Equation ( 8 ), there is a negative exponential relationship between the transmitted light
intensity and the concentration of the suspended iodine particles. Figure 6 a shows that the grayscale
value and VC concentration have a logarithmic relationship. You may need to adjust the volume that
you pipet in, based on your calculations of the approximate amount of vitamin C present (pipets with
the following volumes will be available for you to use: 1.00, 2.00, 3.00, 4.00, 5.00, 10.00, 25.00 mL).
If you use a different volume of sample, you will also need to change the amount of the sulfuric acid
solution used such that the proportion between the solutions remains the same. In a completely
randomized design with 5 treatments and 5 replicates, 300 day-old commercial mail broiler chicks
(ROSS) were divided into 25 groups, 12 chicks per group. Record the volume of DCIP needed to
oxidize all of the ascorbic acid. Three groups of randomly selected experimental data were used to
obtain a set of average data. 3.1. Experiment Data and Analysis The color component data (R, G, B,
L, a, and b), grayscale, and log-grayscale of the suspension image were obtained by the measuring
device and analysis software. Also remember that if you wish to look at the change in vitamin C over
time then you must plan ahead to allow for this. According to International Union of Pure and
Applied Chemistry (IUPAC) regulation, the LOD and LOQ were calculated as LOD. The sampling
area was sealed by a box that was made of light-excluding black acrylic, the thickness of which was
3 mm to isolate external light and, thus, avoid the effect of external light on the sample. Note that
from the first issue of 2016, this journal uses article numbers instead of page numbers. By continuing
we’ll assume you’re on board with our cookie policy. The pipettes must be carefully cleaned by
distilled water and rinsed with the solution to be taken up. The deionized water obtained from
Shanghai Jingchun Water Technologies Co., Ltd. was used throughout the experiment. Calculate the
uncertainty in the amount of vitamin C in your sample at the 95% confidence limit. This is because
vitamin C plays an important role in maintaining human health.