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In the presence of small amounts of iodine and triiodide the reaction between itself and starch is
freely reversible. Scurvy leads to the formation of spots on the skin, bleeding from the mucous
membranes such as nosebleeds, painful joints and bleeding gums due to poor collagen formation in
the body. Closed shoes should be worn in the lab to provide adequate protection in case of any
mishaps. As all of the amounts measured in this experiment are similar (67.0%, 64.8% and 65.0%). It
seems to suggest that the error is a result of the experimental procedure for measuring the quantity of
vitamin c and not in the manner that it has been carried out. In the dry state, it is reasonably stable in
air, but in solution it rapidly oxidizes. Vitamin C in the body and an experiment to measure Vitamin
C in cabbage. For Later 0 ratings 0% found this document useful (0 votes) 12 views 7 pages Science
Fair Vitamin C Board 1231210832411829 2 Uploaded by corevortex AI-enhanced title ok science
Full description Save Save science-fair-vitamin-c-board-1231210832411829-2 (1. However, the back
titration is used instead of direct titration with I 2 because I 2 can be easily vapourize thus the
molarity of I 2 solution is difficult to control, thus using 'standard' I 2 solution for titration is
impossible. After that, carry out the procedure above to determine the vitamin C (Ascorbic
acid)content through titration.Finally, compare the result with the value obtained in the experiment
this time(which serves as control). In addition, if DCPIP solution is stored for any length of time it
should be kept in a dark-coloured container, which was not the case. From the graph, we can deduce
that the volume needed to decolourise 0.5ml of 1% DCPIP solution is inversely proportional to the
concentration of vitamin C. Once both solutions were freshly made the difference was obvious; the
fresh DCPIP solution was blue rather than purple and the test worked much better. Iodine is very
weakly soluble in the water, and can be easily lost from the solution due to its volatility. In the
presence of small amounts of iodine and triiodide the reaction between itself and starch is freely
reversible. Measuring Vitamin C content in different substances. Also colour blind people might not
be able to tell the difference between the blue at the start and the pink at the end. The juices
containing the most Vitamin C titrated the. The fresh juices have a greater range; one brand (Asda
Smooth) has less vitamin C than any of the concentrates but squeezed orange has more. Orange juice
contains the highest amount of vitamin C when compared with lime juice and star fruit juice.
Moreover, the indicator is not affected by the presence of (I - ). Vitamin C is important in the
formation of connective tissues in the body such as bones, teeth, skin and lining of blood vessels.
The risks evaluated by the student and the improvements suggested are quite average, and the
student could have identified other risks associated with the experiment and better improvements.
Use the pipette to put 1 ml DCPIP solution in each test tube. The equivalence point and colour
changes of indicators are not instant and it is difficult to access when the reaction has finished.
Without knowing how the juices were produced, it is not possible to draw any meaningful
conclusions. All solutions were prepared either by squeezing the juice out of fruits and vegetables or
by filtering the juice from the carton. Vitamin C helps to prevent deficiency diseases, in particular
scurvy, and is abundant in citrus fruits such as lemons, limes and oranges. After that, carry out the
procedure above to determine the vitamin C (Ascorbic acid)content through titration.Finally, compare
the result with the value obtained in the experiment this time(which serves as control). Therefore the
iodine must be diluted first with the titrant,sodium to ensure no highly stable complex is formed. It
occurs as a white or slightly yellow crystal or powder wit h a slight acidic taste.
As the concentration of vitamin C increases, the volume required decreases. Use the pipette to put 1
ml DCPIP solution in each test tube. As soon as the DCPIP becomes decolourised, note the number
of drops added. The build up of these by-products over time is largely responsible for the aging
process and can contribute to the development of various health conditions such as cancer, heart
disease, and a host of inflammatory conditions like arthritis. Ascorbic acid is also important
biologically as it is used as a cofactor in enzymatic reactions (no less than 8 reactions have been
identified). In the dry state, it is reasonably stable in air, but in solution it rapidly oxidizes. This
practical would suit students who are studying REDOX chemistry in AS-A2 Cambridge or A-level
courses. These studies show that human body could use only a limited amount of vitamin C, the rest
of vitamin C is excreted. Upload Read for free FAQ and support Language (EN) Sign in Skip
carousel Carousel Previous Carousel Next What is Scribd. Pauling concludes that if humans were
able to produce their own vitamin C equivalent to that produced by other species, it would be several
thousand milligrams per day. Antioxidants are nutrients that block some of the damage caused by
free radicals, which are by-products that result when our bodies transform food into energy. It is
thought that ascorbic acid reacts with these free radicals preventing them from causing damage to
cells. He also argue that this can lesson the likelihood of having colon cancer. It actually took around
5 ml vit C solution before any visible reaction took place at all, and upwards of 30 ml for the DCPIP
to lose its colour completely. If starch is placed in the solution the iodine and triiodide molecules will
react with starch to form a blue-black complex. This is due to the fact that least volume of orange
juice is needed to decolourise and reduce the blue DCPIP solution. This resource hasn't been
reviewed yet To ensure quality for our reviews, only customers who have purchased this resource can
review it Report this resource to let us know if it violates our terms and conditions. Repeat the test
for test tubes 2 and 3, noting how much vit C solution is required. Repeat the test for test tubes 2
and 3, noting how much juice is required. The risks evaluated by the student and the improvements
suggested are quite average, and the student could have identified other risks associated with the
experiment and better improvements. It is reduced by an equal amount of vitamin C to a colourless,
or sometimes pink, compound solution. In the presence of small amounts of iodine and triiodide the
reaction between itself and starch is freely reversible. Furthermore, data from manufacturers together
with researched values for vit. A person's diet must provide all the necessary vitamins. Although it's
so useful, now it is still too soon to say that whether Pauling is right or not. The response is written in
a very clear and concise way. It is difficult to identify how old fruit is due to the manner in which it
is transported from its country of origin. Our range of laboratory equipment includes Lab
Equipments, Lab Spectrophotometer, Laboratory Autoclave, Friability Test Apparatus, Laboratory
Refractometer, Polarimeter Instrument, Laboratory Colorimeter, pH Meter, Microscope etc. We
produce the pure final products from sorbitol in a series of biotechnical, chemical processing and
purification steps. Therefore the iodine must be diluted first with the titrant,sodium to ensure no
highly stable complex is formed.
However, as with the commercial orange juice, some commercial fruit juices may have more vitamin
C than fresh fruit juice because they may have been fortified, that is to manufacture a product with a
higher vitamin and mineral content than before it is refined. Steem POD Team WORLD OF XPILAR
Comunidad Latina AVLE. We then took the mixture and filtered it through filter paper into a
beaker. C will complete the reaction sooner than juices with less Vitamin C. I believed. After that,
carry out the procedure above to determine the vitamin C (Ascorbic acid)content through
titration.Finally, compare the result with the value obtained in the experiment this time(which serves
as control). Perhaps experiments could be done with different light or temperature conditions. Thus,
we need a constant supply of vitamin C in our diet. With this in mind I would suggest that this
experiment is in adequate to measure vitamin c and other methods should be employed. Thus, the
fruit juices should only be prepared right before beginning the experiment to ensure the freshness of
the fruit juices and that the vitamin C content of the fruit juices are highest during the experiment.
Hence, compare this data with the manufacturer's specification. More juices could be tested - 10
concentrates and 10 fresh juices perhaps - and the test could be carried out more than 3 times; the
larger the experiment, the more likely a valid conclusion can be reached. The response is written in a
very clear and concise way. However despite this the quantity of vitamin c in each of the samples
was significantly below published data. Use the syringe to drop 0.1ml juice into test tube 1, then
gently shake the test tube. By using a knife to cut, the amount of vitamin C dissolved in the distilled
water is inaccurate and frequently less than the theoretical amount as parts of the vitamin C tablet is
crushed into powder when force is applied on the tablet. Write a review Update existing review
Submit review Cancel It's good to leave some feedback. When dry, it's reasonably stable in air but in
solution, it oxidizes rapidly in the presence of air. After that, carry out the procedure above to
determine the vitamin C (Ascorbic acid)content through titration.Finally, compare the result with the
value obtained in the experiment this time(which serves as control). However as the concentration of
iodine and triiodide increases the reaction between starch molecules is relatively strong and becomes
less reversible. Scurvy leads to the formation of spots on the skin, bleeding from the mucous
membranes such as nosebleeds, painful joints and bleeding gums due to poor collagen formation in
the body. Write a review Update existing review Submit review Cancel It's good to leave some
feedback. Report this Document Download now Save Save VITAMIN C For Later 0 ratings 0%
found this document useful (0 votes) 117 views 3 pages Vitamin C Uploaded by Drkrishnasarma
pathy AI-enhanced title PROCESS FOR PREPARATIN OF VITAMIN-C The synthesis of ascorbic
acid was achieved by Reichstein in 1933, followed by industrial production of ascorbic acid two
years later by Roche. Also the state of decolourization is not precise as, again, the human eye can be
deceiving. The empirical formula is C6H806, and the molecular weight is 176.13. Today, vitamin C
identical to that occurring in nature is produced on a very large industrial scale. However, when the
all the vitamin C is oxidized, iodine and triiodide will be present. The water-soluble vitamin C is
probably the most well -known vitamin. As soon as the DCPIP becomes decolourised, note the
number of drops added. Join our team of reviewers and help other students learn. Report this
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found this document useful (7 votes) 19K views 45 pages Vitamin C Chemistry Coursework
Uploaded by SiwelEitak A2 Salters B Chemistry Coursework.
Vitamin C. Core Practical. Throughout this investigation we will aim to fi. Our customer service
team will review your report and will be in touch. ?1.00 (no rating) 0 reviews BUY NOW Save for
later ?1.00 (no rating) 0 reviews BUY NOW Save for later Last updated 22 February 2018 Share this
Share through email Share through twitter Share through linkedin Share through facebook Share
through pinterest D-rogchem's Shop 4.42 18 reviews Not the right resource. Vitamin C is important
in the formation of connective tissues in the body such as bones, teeth, skin and lining of blood
vessels. Another method would be to dissolve a whole tablet in 100ml distilled water in a beaker
before diluting the solution accordingly by using a measuring cylinder to measure out the exact
amount of water required. However despite this the quantity of vitamin c in each of the samples was
significantly below published data. Reviews Select overall rating (no rating) Your rating is required
to reflect your happiness. There is no risk of overdose on vitamin C from food but you can overdose
on vitamin C tablets, this causes indigestion, diarrhea, sickness and insomnia. These include the
synthesis of collagen, a deficiency of which leads to the most notable disease of vitamin c loss,
scurvy. It is reduced by an equal amount of vitamin C to a colourless, or sometimes pink, compound
solution. It implies that the number of mole of vitamin c reacted with I 2 decreases. Solution can also
be left behind in flasks as it is transferred from one to the other. Solution can also be left behind in
flasks as it is transferred from one to the other. Closed shoes should be worn in the lab to provide
adequate protection in case of any mishaps. Therefore trying to measure the amount of vitamin c in
two different fruits can not be accurate as you do not know the age; as a result an adequate
comparison cannot be made. Even before its discovery in 1932, physicians recognized that there
must be a compound in citrus fruits preventing scurv y, a disease that killed as many as two million
sailors between 1500 and 1800. No changes were made to the content of the solution. During the
experiment, when vitamin C or fruit juice is added drop by drop into the test tube containing DCPIP
solution, the test tube should not be shaken too vigorously as this may introduce oxygen into the
solution causing the oxygen to oxidise the DCPIP and partially restore the dark colour of the DCPIP
solution resulting in an inaccurate observation of the volume of vitamin C or fruit juice required to
decolourise the DCPIP solution. Gently shake the test tube and note your observations. By first
testing a known concentration of vitamin C solution it should be possible to calculate the vitamin C
content in each fruit juice. Determination of the Amount of Vitamin C in a Commercial Tablets.
Upload Read for free FAQ and support Language (EN) Sign in Skip carousel Carousel Previous
Carousel Next What is Scribd. These are common weaknesses in investigation reports and students
aiming for the higher grades need to address all these issues in their work. Report this Document
Download now Save Save science-fair-vitamin-c-board-1231210832411829-2 (1. In order for the
starch solution to change colour, both starch and iodide must be present. Ascorbic acid is also
important biologically as it is used as a cofactor in enzymatic reactions (no less than 8 reactions have
been identified). Therefore concentrated fruit juice has had its water removed (probably for ease of
transportation) then had water re-added (reconstituted) before packaging. However, when all
available iodine has been reacted, the blue-black complex becomes colourless which signals the end-
point. These items can be treated in various ways such as steamed, boiled, or soaked in water if you
wish. Another consideration would be the addition of ascorbic acid by the manufacturer. This
resource hasn't been reviewed yet To ensure quality for our reviews, only customers who have
purchased this resource can review it Report this resource to let us know if it violates our terms and
conditions.
Using the syringe, drop 0.1ml ascorbic acid solution (vit C) into test tube 1, then gently shake the
test tube. Pauling and his supporters argue that humans are one of five species that do not synthesize
vitamin C. Our range of laboratory equipment includes Lab Equipments, Lab Spectrophotometer,
Laboratory Autoclave, Friability Test Apparatus, Laboratory Refractometer, Polarimeter Instrument,
Laboratory Colorimeter, pH Meter, Microscope etc. The water-soluble vitamin C is probably the most
well -known vitamin. The decision was made to improvise, and use the juice of an orange, squeezed
just prior to testing. It is difficult to identify how old fruit is due to the manner in which it is
transported from its country of origin. The build up of these by-products over time is largely
responsible for the aging process and can contribute to the development of various health conditions
such as cancer, heart disease, and a host of inflammatory conditions like arthritis. There were minor
differences for some of the solutions, highlighting the need for multiple tests. This resource hasn't
been reviewed yet To ensure quality for our reviews, only customers who have purchased this
resource can review it Report this resource to let us know if it violates our terms and conditions. Join
our team of reviewers and help other students learn. Repeat the test for test tubes 2 and 3, noting
how much juice is required. See other similar resources ?3.00 (no rating) 0 reviews BUY NOW Save
for later Not quite what you were looking for. Consequently, as the calculation of number of mole of
S 2 O 3 2- depends on the actual number of mole of I 2., the number of mole of S 2 O 3 2-
calculated will be lower than the actual one. However, it should be added only at the time when the
solution becomes very pale yellow (nearly colourless) because the complex form between starch and I
2 at a high concentration is irreversible. Another consideration would be the addition of ascorbic acid
by the manufacturer. Collagen fibre plays an important role in teeth, cartilage, skin, blood vessels,
etc. The amount of Vitamin c in the samples that I have measured was 67.0%, 64.8% and 65.0%
respectively of the published data for the quantity of vitamin c, (as shown below). These include the
syntheses of collagen, neurotransmitters, tyrosine carnitine and metabolism of microsome 2. These
results match with that of published data for the expected amount of vitamin c in fruits.
BiochemNetworks. Ethereal theme. Powered by Blogger. Upload Read for free FAQ and support
Language (EN) Sign in Skip carousel Carousel Previous Carousel Next What is Scribd. The vitamin
C tablet should have been pounded first with a pestle and mortar and the powdered vitamin C
should be weighed out accordingly using an electronic balance to before being dissolved in 100ml
distilled water to produce the vitamin C solutions of different concentrations. Furthermore, the fruit
juice is added with preservatives, colourings and other additives which affected the vitamin C
content of the fruit juices. Therefore concentrated fruit juice has had its water removed (probably for
ease of transportation) then had water re-added (reconstituted) before packaging. A person who is
lacking in vitamin C is also more likely to be affected by cardiovascular diseases as atherosclerosis
may develop when the lining of the artery is damaged. It is thought that the absence of an enzyme l-
gulonolactone oxidase from liver cells 1 prevents humans from converting glucose into ascorbic acid.
Upload Read for free FAQ and support Language (EN) Sign in Skip carousel Carousel Previous
Carousel Next What is Scribd. Also the state of decolourization is not precise as, again, the human
eye can be deceiving. It implies that the number of mole of vitamin c reacted with I 2 decreases.