Struggling with writing your thesis on Alpha Amylase research papers? You're not alone.

Crafting a
comprehensive and insightful thesis can be an arduous task, requiring extensive research, critical
analysis, and effective communication of findings. From formulating a research question to
conducting experiments and interpreting results, the journey to completing a thesis is often filled
with challenges and uncertainties.

One of the primary difficulties encountered by many students is the sheer volume of information
available on the subject. With a vast array of research papers, studies, and scholarly articles to sift
through, synthesizing relevant data and identifying key insights can be overwhelming. Additionally,
ensuring accuracy and credibility in citing sources is crucial, adding another layer of complexity to
the writing process.

Moreover, the technical nature of Alpha Amylase research papers adds to the complexity.
Understanding biochemical principles, experimental methodologies, and statistical analyses requires a
high level of expertise and proficiency. For students without a strong background in biochemistry or
related fields, grasping these concepts can pose significant challenges.

Time constraints further exacerbate the difficulty of writing a thesis. Balancing academic
responsibilities, extracurricular activities, and personal commitments leaves little room for extensive
research and writing. As deadlines loom closer, the pressure to deliver a high-quality thesis
intensifies, often leading to stress and anxiety among students.

In light of these challenges, seeking assistance from professional writing services can be a viable
solution. ⇒ BuyPapers.club ⇔ offers expert guidance and support to students embarking on their
thesis-writing journey. With a team of experienced writers specializing in various disciplines,
including biochemistry and molecular biology, ⇒ BuyPapers.club ⇔ provides customized
assistance tailored to your specific needs.

By entrusting your thesis to ⇒ BuyPapers.club ⇔, you can alleviate the burden of writing while
ensuring the highest standards of quality and professionalism. From conducting thorough research to
crafting well-structured arguments, their writers are committed to delivering exceptional results that
meet your academic requirements.

Don't let the complexities of writing a thesis on Alpha Amylase research papers hold you back. Take
advantage of ⇒ BuyPapers.club ⇔'s expertise and experience to navigate the challenges effectively
and achieve academic success. Order now and embark on a journey towards a stellar thesis that
showcases your knowledge and proficiency in the field.
Identical residues in the three sequences are shaded in white, residues that coincide in two of the
sequences or do not coincide at all are shaded in pink and red, respectively. Adobe Express Go from
Adobe Express creation to Issuu publication. IOSR Journals Enzyme amylases Enzyme amylases
vaishalidandge3 Isolation and Characterization of Amylase from Fungal Strain Isolation and
Characterization of Amylase from Fungal Strain AnkitKushwaha57 Effect of Different Physico-
Chemical Parameters on Production ofAmylase by Ba. Aim: The aim of this study is to detect the
activity of alpha-amylase from bacteria isolated from soil. Figure: 5 Phylogenetic tree of Bacillus
mojavensus. Department of Genetics and Bioengineering, School of Sciences and Engineering, Fatih
University Istanbul. Following the overnight incubation of the culture media, the bacteria were able
to degrade the media. Therefore, intra- and extracellular proteins from haloarchaea are specially
adapted to work properly at high salt concentrations. A comparison with the optimal parameters
reported for amylases of other related haloarchaea is summarized in Table 4. Results: Positive
response to alpha-amylase activity by the soil bacteria was observed by the formation of clear.
K.Venkatanand Reddy, V.Haritha, Chv.Siva kumar, J.Ganesh. Serum level of amylase and lipase in
various stages of chronic renal insufficiency. The funders had no role in the design of the study; in
the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision
to publish the results. Alpha amylase (?-Amylase) is a faster-acting enzyme than beta amylase (?-
amylase). The possible existence of divalent metals in the partially purified enzymatic preparations
makes it difficult to obtain accurate conclusions on the effects of these metals on the amylase
activities of Haloarcula sp. HS. However, the strong inhibition observed in the presence of the metal
chelating agent EDTA confirmed the divalent cation dependence of both, extracellular and cell-
associated amylase activities, which decreased to 39% and 33%, respectively, in the presence of
EDTA. The most characteristic feature of halophilic enzymes is their ability to operate under very
high salinities. The fungal amylases are likely to denature at the high temperatures (Seager and
Slabaugh 311). HS Isolated in the Odiel Marshlands. Biology. 2021; 10(4):337. School of Sciences,
Department of Biology, Saadatu Rimi College of Education, Kunbotso, Kano State-Nigeria. Alpha-
amylases are hydrolytic enzymes that are widespread in nature being found in microorganisms.
Variation in temperature has different effects on working of enzymes. HS, exhibit a strong inhibition
in the presence of the metal chelating agent EDTA ( Figure 6 ). These proteins are produced by a
wide variety of organisms and are supposed to be one of the most valuable industrial enzymes. The
extracellular and cellular starch-degrading activities of the selected archaea were characterized,
revealing different optimal parameters and modes of action. Production of High Activity Amylase
from Trichoderma. In this context, detergent-stable amylases were recently found in Haloterrigena
tukmenica, Halorubrum xijiangense, and Haloferax sp. This step should be repeated while starch
solution labelled S. Tropical Medicine and Infectious Disease (TropicalMed). This means that
bacterial amylases would work at a higher temperature than fungal amylases. This experiment was
made up of setting up a starch control group placing bacterial and fungal amylases in starch and
placing them in various temperatures.
Secretarial Performance and the Gender Question (A Study of Selected Tertiary. An enzyme is a
complex protein whose function is to speed up chemical reactions through catalysis. Video Say more
by seamlessly including video within your publication. The rest of the proteins identified in the
extracellular fraction were membrane-ligated proteins, probably from broken cell remains. C while,
the other specie (Bacillus subtilus) shows 30o. Urine and blood amylase levels may also be
moderately elevated with a variety of other conditions, such as ovarian cancer, lung cancer, tubal
pregnancy, acute appendicitis, diabetic ketoacidosis, mumps, intestinal obstruction, or perforated
ulcer, but amylase tests are not generally used to diagnose or monitor these disorders. Gram’s staining
technique was employed for the characterization of the colonies so as to know the. Despite the low
percentage of sequence identity that the three amylases of Haloarcula sp. In order to isolate and
identify the group of bacteria that were responsible for alpha-activity, the. The shape of the enzyme
changes rapidly to allow the substrate to bind. Food Technol. Biotechnol, Vol. 44, pp. 173-174, 2006.
Regarding the physicochemical characteristics, the three alpha-amylases of Haloarcula sp. Paper
industry, Textile industry, Alcohol industry, Detergent industry, Glucose industry, Bread and
Chapatti. Low serum amylase levels are observed in individuals with chronic pancreatitis 7. Figure:
10 Graph of pH gradient with respect to relative enzymatic activity for Bacillus subtilus. This article
is an open access article distributed under the terms and conditions of the Creative Commons
Attribution (CC BY) license ( ). Both, extracellular and cellular, enzymatic preparations were
incubated with 1 mg of starch in the standard conditions, described in Material and Methods,
excepting that the incubation time was fixed at 2 h. These features, joined to their stability under a
wide range of surfactants, make them suitable for industrial applications. This means that fungal
amylase has a lower optimal temperature compare to bacterial amylase. Among them, bread was
chosen as the substrate for this assay, as it is one of the most abundant food waste products
worldwide. Implementation of Quality Management principles at Zimbabwe Open University (. The
three proteins conserve the consensus domains and residues of the ?-amylase family. Amylase
supplement is approved for the uses listed above. Add 2-3 drops of iodine to the first row of the
plate indicated as 0 minutes. Latent associations of low serum amylase with decreased plasma insulin
levels and insulin resistance in asymptomatic middle-aged adults. Moreover, serum amylase levels are
also elevated in other conditions, including diabetic ketoacidosis 5 and renal insufficiency 6. In this
case, the specific activity was increased from 20 U mg ?1 in the crude cell extract to 120 U mg ?1 in
the partially purified preparation, with a purification factor of 6. There are mainly two methods
which are used for production of ?-Amylase on a commercial scale. Comparative Study on
Antimicrobial Activity and Microbial Load of Alternanthe. Avoiding Bad Stats and the Benefits of
Playing Trivia with Friends: PancakesC.
All articles published by MDPI are made immediately available worldwide under an open access
license. No special. Note: Normal value ranges may vary slightly among different laboratories. In this
work by Pallab Kumar Borah (PhD Student, Tezpur University, India; Commonwealth Split-side
PhD Student at University of Leeds, UK) with Dr. Raj Kumar Duary from Tezpur University, India,
it was demonstrated for the first time that water soluble vitamins, such as vitamin B9 and C can
inhibit human pancreatic alpha-amylase. The funders had no role in the design of the study; in the
collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to
publish the results. Amylase supplement is approved for the uses listed above. Amylases are broadly
classified into ? (alpha), ? (beta), and ? (gamma) subtypes, of which the first two have been the most
widely studied. Implications for the Catalytic Mechanism of Glycosidases. In this study, a collection
of haloarchaea isolated from Odiel saltern ponds in the southwest of Spain was screened for their
amylase activity. High retention of enzyme activity over a wide range of temperatures was reported
for other partially purified amylases, such as that from Haloferax sp. Optical density, secreted
proteins, and starch concentration were measured along the full cultivation time. The
physicochemical parameters of the new alpha-amylases, with low isoelectric point and negative net
charge ( Table 3 ) also meet the usual characteristic of haloarchaeal enzymes, as reported by other
authors. The result of a phylogentic analysis is expressed in a phylogenetic tree where closely related
organisms have a. We found that the new strain exhibited a high amylolytic activity when cultured in
the presence of starch; this activity was higher in a minimal medium with ammonium acetate ( Figure
2 ). Journal of Theoretical and Applied Electronic Commerce Research (JTAER). Further studies aim
to decipher the function of a hypothetical ancestral gene and to increase our understanding of the
biochemical behavior of these polyextremophilic enzymes. Applications of these enzymes include
chemical production, bioconversion (biocatalyst), and bioremediation. Effects of Pretreatments and
Drying Methods on Some Anti-nutrients and Proxim. Gout, Urate, and Crystal Deposition Disease
(GUCDD). The rate of an enzyme catalyzed reaction increase until it reaches the optimal
temperature, decreases rapidly after the optimal temperature and stops after an enzyme is denatured.
Fungal sources of ?-Amylase are confined to terrestrial isolates mostly to Aspergillus species and to.
Sequences of the primers employed for the amylase encoding genes amplification. Table: 3Alpha-
amylase Activity Result for Bacillus subtilus. The need for poly-extremotolerant amylases
encouraged their search in extreme environments, where archaea become ideal candidates to provide
new enzymes that are able to work in the harsh conditions demanded in many industrial applications.
Subsequently, the gel was stained with commercial Lugol?s reagent and the appearance of clear
bands revealed the amylase activity. Public Sector Reforms and Outsourcing Services in Nigeria: An
Empirical Evalu. Report this Document Download now Save Save Alpha Amylase For Later 0
ratings 0% found this document useful (0 votes) 86 views 6 pages Alpha Amylase Uploaded by
LaurensiaElsa Alpha amylase Full description Save Save Alpha Amylase For Later 0% 0% found
this document useful, Mark this document as useful 0% 0% found this document not useful, Mark
this document as not useful Embed Share Print Download now Jump to Page You are on page 1 of 6
Search inside document. High and low temperatures have varying effect on the ability of amylases to
break down the starch. The figure: 11 above shows the bacterial alpha-amylase activity at different
temperature using DNS. Compared with diabetes mellitus patients, healthy individuals have a higher
?-amylase level in the serum and a lower blood glucose level. Performance of different substrates on
growth, yield and biological efficienc.
In this case, levels returning to normal may not indicate that the source of damage has been resolved.
Furthermore, the potential applicability of the amylase enzymes of this new haloarchaea on the
treatment of bakery waste was assessed and compared with a commercial amylase. 2. Materials and
Methods 2.1. Screening and Selection of Amylase Producing Haloarchaea The screening of amylase-
producing haloarchaea was performed by detection of the extracellular amylase activity of the
isolates on starch agar plates, with 20% NaCl. As it is obtained from the result, majority of the soil
bacteria that show alpha-amylase activity belong. They are categorized into exo-acting, endo-acting
and debranching. Redwood City, Calif: Benjamins Cummings Publications, 2004. Print. Next
Article in Journal Integration of Multiple Resolution Data in 3D Chromatin Reconstruction Using
ChromStruct. Optimization of Cultural Parameters for Cellulase Enzyme Production from Fung.
Optimal parameters reported for ?-amylase activity in haloarchaea. Implications for the Catalytic
Mechanism of Glycosidases. Figure: 8 Graph of pH gradient with respect to relative enzymatic
activity for Bacillus mojavensus. Table 2: Product concentrations at various temperatures against time
of fungal amylase. These features, joined to their stability under a wide range of surfactants, make
them suitable for industrial applications. The ??amylase is intended to be used in starch processing
for the production of glucose syrups and distilled alcohol production 2. Finally, a multispecies study
was carried out to compare the degree of conservation among the amylases reported and those from
different haloarchaeal members. The functional significance of ?-amylase-binding proteins in oral
colonization by streptococci is important for understanding how salivary components influence oral
biofilm formation by these important dental plaque species. HS ( Figure 1 B). The hyperthermophilic
archaea Methanococcus vulcanus was used as an outgroup and the bootstrap was set at 1000
replicates. The shape of the enzyme changes rapidly to allow the substrate to bind. Emotional
Intelligence and Work Performance Relationship: A Study on Sales Pe. C) out of which two species
(Bacillus mojavensus and Paenibacillus sp B3a) show. Most authors agree that amylase production is
growth-associated and is strongly induced by starch. HS was grown in a two-stage culture with
starch (3 g L ?1 ), as previously described ( Figure 3 ). The slides were flooded with the crystal violet
solution (primary stain) for 1 minute and then washed. An Examination of Effectuation Dimension as
Financing Practice of Small and M. A small proportion of glucose was found in the assay catalyzed
by the cell extract. The absorbed proteins were eluted using a linear gradient of NaCl from 0 to 500
mM and a final washing with NaCl 1 M, with a flow rate of 15 mL h ?1. Consult a doctor for
medical advice, treatment or diagnosis. Journal of Theoretical and Applied Electronic Commerce
Research (JTAER). The alignment of the three obtained protein sequences revealed that the
extracellular amylase only presented a 17% identity with the cell-associated amylases, which in turn
showed a 38% identity between them. Comparative Study on Antimicrobial Activity and Microbial
Load of Alternanthe. Further studies aim to decipher the function of a hypothetical ancestral gene
and to increase our understanding of the biochemical behavior of these polyextremophilic enzymes.
Different times were used to obtain reliable results (Seager and Slabaugh 308). Based on the above
mentioned, it seems that there were diverse amylases with probably different modes of action, which
to our knowledge, are not yet deeply elucidated. HS Isolated in the Odiel Marshlands. Biology.
2021; 10(4):337. Recently, it was reported that when the blood glucose level is high in type 2
diabetes mellitus, serum amylase activity is significantly low 16. C after which the bacterial growth
was detected in form of colonies. HS and showed amylase activities in both, the cellular and the
extracellular extracts. Elevated serum amylase and lipase in pediatric diabetic ketoacidosis.
Glucoamylases (?-amylases) are enzymes breaking glucosidic bond to produce solely ?-glucose and
?- amylases. The examples above show the common measurements for results for these tests. The
results ( Table 2 ) revealed that starch degradation was almost complete in all cases, being especially
efficient in the case of the extracellular amylase extract, with a remaining starch of only 1.7%. The
main product obtained with the three enzymatic sources was maltose, which represents between
73.8% and 86.1% of the total carbohydrate content in the reaction mixtures. If you would like more
information, ask your doctor. This ratio is used to assess kidney function because improper function
can result in a slower rate of amylase clearance. About 1g of dinitrosalicylic acid (DNS) (Merck,
Germany) was dissolved in 50ml of distilled water. To. Authors have declared that no Competing
interest exist. The sequences obtained were translated to protein and both, DNA and protein
sequences, were compared to those available at National Center for Biotechnology Information
(NCBI) databases, using the Basic Local Alignment Search Tool (BLAST). Nonetheless, as it is
shown in their predicted three-dimensional ( Figure 7 ) and secondary structures ( Supplementary
Material, Figure S3 ), the three amylase sequences from Haloarcula sp. This study allowed the
identification of three amylase sequences (two were exclusively cell-associated and one was also
found in the extracellular medium) with high homology to amylases of other haloarchaeal species,
and the typical alpha-amylase conserved regions. Table 1: Product concentrations at various
temperatures against time of bacterial amylase. Biochemical Characterization of the Amylase
Activity from the New Haloarchaeal Strain Haloarcula sp. B.subtilis,
B.cereus,B.megaterium,B.coagulans, B.polymyxa, B.stearothermophilus, are widely used for.
Comparative Study on Antimicrobial Activity and Microbial Load of Alternanthe. Emotional
Intelligence and Work Performance Relationship: A Study on Sales Pe. Therefore, a two-step culture
was set up, in which a large amount of biomass was obtained in a rich medium, followed by transfer
to a minimal medium with starch, to induce the production of amylase activity ( Figure 3 ). Lane
M—molecular mass marker in kDa, lanes 1 and 2—crude extract, lanes 3 and 4—partially purified
crude extract, and lanes 5 and 6—concentrated supernatant. This indicates that increase in substrate
concentration increases the rate of reaction to a given point. To get a further insight into the identity
of these starch-degrading enzymes, the proteome composition of the partially purified cell-free
supernatant and the cellular extracts was analyzed by tryptic digestion, followed by nano-liquid
chromatography coupled to an electrospray ionization tandem mass spectrometry system. Figure:13
Graph of temperature gradient with respect to relative enzymatic activity for Paenibacillus sp B3a.
Digital Sales Sell your publications commission-free as single issues or ongoing subscriptions. This
means that the amylase was able to break down the starch that was present in the solution. However,
the extreme conditions required for many industrial operations limit the applicability of most
amylases found in nature.
Amylase or alpha amylase can specifically cleave the O-glycosidic bonds in starch, glycogen and
several oligosaccharides 11. Organizational Conflicts Management In Selected Organizaions In
Lagos State. In this study, a collection of haloarchaea isolated from Odiel saltern ponds in the
southwest of Spain was screened for their amylase activity. Amylase levels may also be significantly
increased in people with pancreatic duct obstruction and pancreatic cancers. Alpha amylase (?-
Amylase) is a faster-acting enzyme than beta amylase (?-amylase). C after which the bacterial growth
was detected in form of colonies. A high amylase level in the blood may indicate the presence of a
condition affecting the pancreas. In this work, a screening of haloarchaea, isolated from Odiel
salterns in the southwest of Spain, was carried out to select a new strain with a high amylase activity.
Add Links Send readers directly to specific items or pages with shopping and web links. Seaweed
Based Bio Polymeric Film and Their Application: A Review on Hydrocoll. Screenings were done in
triplicates. 2.2. Identification of the Selected Microorganism Genomic DNA of the isolated amylase-
producing strain was purified using the GeneJET Genomic Purification kit (Thermo Fisher Scientific,
Waltham, MA, USA), following the manufacturer’s instructions. Social Media and Small Businesses:
A Combinational Strategic Approach under t. HS ( Figure 8 ). Most available archaeal amylase
sequences were from the thermophilic archaea and could work at very high temperatures, which was
of great industrial interest. Isolation, identification and screening of alkaline protease from
thermophili. The subchronic toxicity was assessed by means of a repeated dose 90?day oral toxicity
study in rodents. Avoiding Bad Stats and the Benefits of Playing Trivia with Friends: PancakesC.
Amylase is usually present in the blood and urine in small quantities. Above the optimal temperature
the rate of enzyme activity reduces. Three-dimensional predicted structures of the three amylase
sequences identified in Haloarcula sp. The funders had no role in the design of the study; in the
collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to
publish the results. The obtained cell extract was loaded onto a DEAE Sephacel TM column
equilibrated with the same phosphate buffer. In this study, a collection of haloarchaea isolated from
the saltern ponds of the Odiel Marshlands was screened for their amylolytic activity, and the one
that exhibited the highest activity was selected and identified on the basis of its 16S rRNA coding
gene. To test the influence of different metals on cell-associated and extracellular amylase activities
MgSO 4, CaCl 2, CuCl 2, FeCl 2, FeCl 3, or EDTA (ethylenediaminetetraacetic acid), were added to
the reaction mixture, in a final concentration of 10 mM. Amylase supplement Pancreatic enzyme
supplements are used by people whose bodies do not make enough amylase enzyme to digest their
food such as patients with cystic fibrosis or chronic pancreatitis; or patients who have had their
pancreas removed or who have had gastrointestinal bypass surgery or suffer from ductal obstruction.
Contrarily, the extracellular amylase activity appeared to be more susceptible to extreme pH, losing
more than 50% of its activity both at low and high pH values. Starch is a polysaccharide made up of
simple sugars (glucose). Among these amylase sequences, different percentages of identity were
found with the extracellular amylases from Haloarcula hispanica N601 (90%), Halomicroarcula
salina (73%), Halapricum salinum (57%), and Haloterrigena turkmenika (40%). Extracellular and
cell-associated relative amylase activities under the presence of different metal ions and EDTA (10
mM) are represented. European Journal of Investigation in Health, Psychology and Education
(EJIHPE). A previous study by Muneyuki et al. 9 of asymptomatic middle-aged adults showed that
low serum amylase levels were associated with decreased basal insulin levels and insulin secretion, as
well as increased insulin resistance.