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The DLS size distribution histogram of Ag NPs was illustrated in Figure 5. We spent close to a year
testing out various ways to develop the particles and found fascinating properties, even in the
synthesis of the particles themselves. Silver nanoparticles have tunable physical and chemical
properties, so it has been studied widely to improve its applicability. TEM micrographs of AgNPs
synthesized by Teucrium apollinis extract as protecting and stabilized ligands. In addition, the
reagents used in green synthesis are non-toxic. In the present study, silver nanoparticles (AgNPs)
were synthesized using various plant extracts like fern, jackfruit, and onion. To browse
Academia.edu and the wider internet faster and more securely, please take a few seconds to upgrade
your browser. This investigation demonstrated that silver nanoparticles with a concentration of 2 ppm
and 4 ppm were not toxic for human healthy cells, but inhibit bacterial growth. Journal of
Otorhinolaryngology, Hearing and Balance Medicine (JOHBM). The positive records of antibacterial
activity ranged between 2.4 and 23.6 AU. Occasionally, as well as, SUDW showed high AU (6.6)
against bacterial community in water from both harbors. The IR bands of a pure stabilized ligand
(teucrium apollinis extract) are shown in the second curve (red line). Journal of Functional
Morphology and Kinesiology (JFMK). The UV-Visible spectrum showed a peak between 417 and
425 nm corresponding to the Plasmon absorbance of the AgNPs. Due to the presence of phenolic
chemicals in the extract plant, the size of the AgNPs seems large. Both the examination of the
particles and the creation of the TEM micrographs were carried out using a JEOL 2100 field
emission gun transmission electron microscope (FEG TEM) set at 100 KV. The reducing agents used
to produce the nanoparticles were from aqueous extracts made from the leaves of various plants.
Nanoparticles are widely used today, particularly in the most advanced scientific materials. The
AgNPs, dispersed in aqueous solution, were prepared at pH 7.2 values. However, with the pH
changing, the AgNP displayed different surface plasmon resonance (SPR) behavior. With more time
passing, the dispersed silver nanoparticles’ sizes seemed to grow larger (bigger red shift noted after
10 weeks). Based on the obtained results, we concluded that nanoparticles synthesized in this way,
show excellent antimicrobial activity and can be used as antimicrobial agent. To browse
Academia.edu and the wider internet faster and more securely, please take a few seconds to upgrade
your browser. The silver nanoparticles were characterized by using UV-Visible spectroscopy, Fourier
transform infrared spectroscopy, transmission electron microscope and X-ray diffraction (XRD)
techniques. Abdussalam-Mohammed W, Mohamed L, Abraheem MS, Mansour MMA, Sherif AM.
Biofabrication of Silver Nanoparticles Using Teucrium Apollinis Extract: Characterization, Stability,
and Their Antibacterial Activities. Chemistry. 2023; 5(1):54-64. This form of synthesis also requires
fewer reagents because plant extracts serve as both reducing and stabilizing agents. In this study,
biogenic AgNPs were synthesized using extracts of different marine algae species, including Ulva
rigida (green alga), Cystoseira myrica (brown alga), and Gracilaria foliifera (red alga), as reducing
and capping agents. So this review deals with the green synthetic procedures and applications of
silver nanoparticles in medicine. It is worth note that increasing the pH value leads to generate
spherical nanoparticles, whereas at lower pH, rods and triangular particle forms are formed.
Methods: The seaweed extract was used for the synthesis of AgNPs Results: The UV-visible
spectroscopy revealed surface plasmon resonance at 450 nm. Figure 6 compares the ATR-FTIR
spectra of teucrium apollinis extract and AgNPs functionalized by the same plant extract.
European Journal of Investigation in Health, Psychology and Education (EJIHPE). The FT-IR
measurements showed the possible functional groups responsible for the formation of nanoparticles.
The antimicrobial activity of AgNPs was checked against pathogens like, E. coli, S. aureus, S.
pyogenes, S. typhi, S. paratyphi A, S. paratyphi B, Shigella and Proteus. Silver nanoparticles were
spherical shaped well distributed without aggregation in solution with an average size of about 40-
80 nm. Journal of Low Power Electronics and Applications (JLPEA). DLS diagrams of AgNPs
functionalized by extract of teucrium apollinis. Some studies illustrated that high acidic and alkaline
pH cause a reduction in the growth of bacterial cells. The surface plasmon resonance peak in
absorption spectra of silver nanoparticle showed an absorption maximum at 420 nm in UV-VIS
spectrometry. In the present study, AgNP had a highly bacterial effect at pH 7.2. Our findings
supported the possibility that different pH levels could be responsible for the differences in AgNP
stability, size, and variations biological effects. The characterization of the AgNPs such as their size
and shape was performed by Atom Force Microscopy (AFM), and Transmission Electron
Microscopy (TEM) techniques which indicated a size range of 3 to 15 nm. Figure 6 compares the
ATR-FTIR spectra of teucrium apollinis extract and AgNPs functionalized by the same plant extract.
The antibacterial activity of biosynthesized silver nanoparticles was carried out by disc diffusion
method against pathogenic bacteria.Results: The UV-visible spectroscopy revealed surface plasmon
resonance at 450 nm. The pH was adjusted to 7.2, and smaller-size AgNPs were produced in
comparison to high and low pH values. The MIC of these AgNPs against E. coli and S. aureus was
determined. For more information on the journal statistics, click here. The AgNP was characterized
using UV-visible absorption spectroscopy (UV-Vis), attenuated Fourier transform infrared (ATR-
FTIR), transmission electron microscopy (TEM), and dynamic light scattering (DLS). This would
indicate the presence of silver nanoparticle. The synthesized AgNPs have shown the best antibacterial
activity against human pathogens such as Staphylococcus aureus and Proteus mirabilis. Silver
nanoparticles have been characterized by UV-Visible spectrophotometer, Fourier-Transform Infrared
spectroscopy (FTIR), X-ray diffractometer (XRD) and High Resolution Transmission Electron
Microscope (HR-TEM). Next Article in Special Issue Hydrothermally Synthesized Hydroxyapatite-
Silica Composites with Enhanced Mechanical Properties for Bone Graft Applications. The AgNP
produced was stable in room condition up to 10 weeks. As a precursor for silver, we used silver salt-
AgNO3. In general, the size distribution of AgNP was obtained by counting and measuring over 160
silver nanoparticles. Nano-size particles of less than 100nm in diameter are currently attracting
increasing attention for the wide range of new applications in various fields of industry. With more
time passing, the dispersed silver nanoparticles’ sizes seemed to grow larger (bigger red shift noted
after 10 weeks). Biofabrication of Silver Nanoparticles Using Teucrium Apollinis Extract:
Characterization, Stability, and Their Antibacterial Activities. The Physiochemical properties,
cytotoxicity, anticancer and antimicrobial activities of the biosynthesized AgNPs were assessed.
Nanoparticles are widely used today, particularly in the most advanced scientific materials. The
minimum inhibitory concentration (MIC) of AgNPs againstS. Journal of Manufacturing and
Materials Processing (JMMP).
The FT-IR measurements showed the possible functional groups responsible for the formation of
nanoparticles. Silver nanoparticles are of silver, which are in the range of 1 and 100 nm in size.
Journal of Theoretical and Applied Electronic Commerce Research (JTAER). Ag NPs show lower
toxicity to human health while Ag NPs show higher toxicity to various microorganisms. For this
reason Ag NPs having scope for medical instruments, antimicrobial application, products for health
care such as scaffolds, burn dressing, water purification, agriculture uses. Feature papers are
submitted upon individual invitation or recommendation by the scientific editors and must receive.
Synthesis of colloidal AgNPs was monitored by UV-Visible spectroscopy. DLS diagrams of AgNPs
functionalized by extract of teucrium apollinis. In order to determine the inhibitory effects of silver
nanoparticles, the test diffusion antibiogram method was used. Their unique properties are due to
their extremely small particle dimension, high surface area, size quantization effect and several other
factors. The results indicated that AgNPs at a concentration of 2 and 4 ppm, inhibited OPEN
ACCESS Int. J. Environ. Res. Public Health 2013, 10 5222 bacterial growth. This plant extraction
reduced Ag (I) to Ag (0) and provided the AgNPs with excellent stability, which is demonstrated by
using UV-Vis, TEM, and DLS results. UV-Vis data collected across the pH range produced by
adding HCl, 0.1 M ( a ) or NaOH, 0.1 M ( b ) to aqueous AgNP functionalized by Teucrium apollinis
extract. Based on the obtained results, we concluded that nanoparticles synthesized in this way, show
excellent antimicrobial activity and can be used as antimicrobial agent. In this paper, we’ve
synthesised silver nanoparticles and tested their antimicrobial activity. X-ray diffraction (XRD)
patterns clearly illustrate the presence of AgClNPs. You can download the paper by clicking the
button above. In addition, the reagents used in green synthesis are non-toxic. Abdussalam-
Mohammed, W.; Mohamed, L.; Abraheem, M.S.; Mansour, M.M.A.; Sherif, A.M. To browse
Academia.edu and the wider internet faster and more securely, please take a few seconds to upgrade
your browser. Perhaps that’s one of the reasons I decided to take up ISTD in my later years. This
study illustrates the bioactivity of biocompatible AgNPs dispersed in an aqueous solution and capped
by teucrium apollinis extract. Journal of Otorhinolaryngology, Hearing and Balance Medicine
(JOHBM). To browse Academia.edu and the wider internet faster and more securely, please take a
few seconds to upgrade your browser. In the present study, the antibacterial activity of AgNPs
against Pseudomonas aeruginosa bacterial strains was examined. Abdussalam-Mohammed, W.;
Mohamed, L.; Abraheem, M.S.; Mansour, M.M.A.; Sherif, A.M. The antibacterial activity of
biosynthesized silver nanoparticles was carried out by disc diffusion method against pathogenic
bacteria. The minimum inhibitory concentration (MIC) of AgNPs againstS. The progress of the
formation of AgNPs can be observed through visual color changes or by using UV-Vis. Journal of
Cardiovascular Development and Disease (JCDD).
Editors select a small number of articles recently published in the journal that they believe will be
particularly. Abdussalam-Mohammed W, Mohamed L, Abraheem MS, Mansour MMA, Sherif AM.
Gout, Urate, and Crystal Deposition Disease (GUCDD). UV-Vis data collected across the pH range
produced by adding HCl, 0.1 M ( a ) or NaOH, 0.1 M ( b ) to aqueous AgNP functionalized by
Teucrium apollinis extract. Abdussalam-Mohammed W, Mohamed L, Abraheem MS, Mansour
MMA, Sherif AM. We also highlight the toxicity and compares Ag NPs with others nanoparticles.
Due to the presence of phenolic chemicals in the extract plant, the size of the AgNPs seems large.
Find support for a specific problem in the support section of our website. This investigation
demonstrated that silver nanoparticles with a concentration of 2 ppm and 4 ppm were not toxic for
human healthy cells, but inhibit bacterial growth. You can find the published paper here for the full
story. It is worth note that increasing the pH value leads to generate spherical nanoparticles, whereas
at lower pH, rods and triangular particle forms are formed. The study confirms the ability of
teucrium apollinis to produce AgNPs with high stability. Cyanobacteria isolates from muthupet
mangrove includes Aphanothece sp, Oscillatoria sp, Microcoleus sp, Aphanocapsa sp, Phormidium
sp, Lyngbya sp, Gleocapsa sp, Synechococcus sp, Spirulina sp with were set in compliance with their
cellular mechanism of nano silver creation, and were investigated by UV-VIS spectrophotometer,
Energy-dispersive X-ray (EDX) and scanning electron microscopy (SEM). To overcome this, the
biological method provides a feasible alternative. AgNP synthesis is confirmed by a strong peak in
the UV-Vis due to surface plasmon resonance (SPR) at 379 nm. The nanoparticles showed highest
inhibition activity on K. AgNP is represented by the first curve (black line). The minimum inhibitory
concentration (MIC) of AgNPs againstS. Journal of Pharmaceutical and BioTech Industry (JPBI).
Abdussalam-Mohammed, W.; Mohamed, L.; Abraheem, M.S.; Mansour, M.M.A.; Sherif, A.M. The
pH was adjusted to 7.2, and smaller-size AgNPs were produced in comparison to high and low pH
values. Study of the Silver Nanoparticle’s Stability The durability of metal nanoparticles over time is
one of the crucial aspects of their suitability for use. The size of the silver nanoparticles in this paper
was controlled by changing the pH values of the reaction system. Gram-negative bacteria, as they are
known, are among the main agents in a nosocomial infection, contributing to an increase in patient
mortality. This plant extraction reduced Ag (I) to Ag (0) and provided the AgNPs with excellent
stability, which is demonstrated by using UV-Vis, TEM, and DLS results. The correlation coefficient
between silver nanoparticles and selected bacterial pathogens revealed that there is a strong negative
correlation with Escherichia coli, S. KeywordsSilver nanoparticles, Green reduction method,
Antibacterial activity. As a precursor for silver, we used silver salt-AgNO3. FT-IR measurements
revealed the possible functional groups responsible for reduction and stabilization of the
nanoparticles.
The effect of varying pH of these extracts on the synthesis of AgNPs was studied, and alkaline
medium was found to decrease the time taken for synthesis. The anti-bacterial activity of AgNPs was
investigated at concentrations between 2 and 15 ppm for Gram-negative and Gram-positive bacteria.
TEM micrograph has shown the formation of silver nanoparticles with the size in the range of 5-50
nm. Abraheem, Mohmeed M.A Mansour, and Akram Mansour Sherif. The Physiochemical
properties, cytotoxicity, anticancer and antimicrobial activities of the biosynthesized AgNPs were
assessed. Silver nanoparticles have unique properties which help in molecular diagnostics, in
therapies, as well as in devices that are used in several medical procedures. The bioactivity of AgNP
stabilized by teucrium apollinis was applied against Pseudomonas aeruginosa and showed good
results. Biofabrication of Silver Nanoparticles Using Teucrium Apollinis Extract: Characterization,
Stability, and Their Antibacterial Activities. Growth studies with optical density (OD) measurements
were performed to evaluate the antibacterial activity of AgNPs in a quantitative manner. Among
them, silver nanoparticles (AgNP) showed various specific properties. Journal of Functional
Morphology and Kinesiology (JFMK). Biofabrication of Silver Nanoparticles Using Teucrium
Apollinis Extract: Characterization, Stability, and Their Antibacterial Activities. Multiple requests
from the same IP address are counted as one view. The surface plasmon resonance peak in
absorption spectra of silver nanoparticle showed an absorption maximum at 420 nm in UV-VIS
spectrometry. The FT-IR measurements showed the possible functional groups responsible for the
formation of nanoparticles. The size of the silver nanoparticles in this paper was controlled by
changing the pH values of the reaction system. In the present study, the antibacterial activity of
AgNPs against Pseudomonas aeruginosa bacterial strains was examined. In addition, AgNP did not
show any decomposition characteristics, such as particle growth or instability. Find support for a
specific problem in the support section of our website. The synthesized AgClNPs were tested for its
antibacterial activity and it was found to cause considerable amount of deterioration to bacterial
cells, when examined using electron microscope and cell viability analysis. Silver nanoparticles
possess a wide variety of antibacterial, antifungal, and antiviral properties. To overcome this, the
biological method provides a feasible alternative. With more time passing, the dispersed silver
nanoparticles’ sizes seemed to grow larger (bigger red shift noted after 10 weeks). Silver
nanoparticles have been known to have antimicrobial properties. The positive records of antibacterial
activity ranged between 2.4 and 23.6 AU. Occasionally, as well as, SUDW showed high AU (6.6)
against bacterial community in water from both harbors. The characterization of the AgNPs such as
their size and shape was performed by Atom Force Microscopy (AFM), and Transmission Electron
Microscopy (TEM) techniques which indicated a size range of 3 to 15 nm. The X-ray diffraction
analysis showed that the particles were crystalline in nature. You can find the published paper here
for the full story. The synthesized materials were also evaluated for their antibacterial activity against
Gram positive and Gram negative bacterial strains. Measurements were carried out in quartz cuvettes
(1 cm light path).
Silver nanoparticles have tunable physical and chemical properties, so it has been studied widely to
improve its applicability. In this paper, we’ve synthesised silver nanoparticles and tested their
antimicrobial activity. This form of synthesis also requires fewer reagents because plant extracts serve
as both reducing and stabilizing agents. The FT-IR measurements showed the possible functional
groups responsible for the formation of nanoparticles. The synthesized materials were also evaluated
for their antibacterial activity against Gram positive and Gram negative bacterial strains. This is due
to the presence of phenolic compounds in the teucrium extract. In addition, the reagents used in
green synthesis are non-toxic. Ag NPs show lower toxicity to human health while Ag NPs show
higher toxicity to various microorganisms. For this reason Ag NPs having scope for medical
instruments, antimicrobial application, products for health care such as scaffolds, burn dressing,
water purification, agriculture uses. The X-ray diffraction analysis showed that the particles were
crystalline in nature. We also highlight the toxicity and compares Ag NPs with others nanoparticles.
In this study, biogenic AgNPs were synthesized using extracts of different marine algae species,
including Ulva rigida (green alga), Cystoseira myrica (brown alga), and Gracilaria foliifera (red alga),
as reducing and capping agents. Among all these methods green synthesis is non-toxic, eco-friendly
and cost effective. To browse Academia.edu and the wider internet faster and more securely, please
take a few seconds to upgrade your browser. Cyanobacteria isolates from muthupet mangrove
includes Aphanothece sp, Oscillatoria sp, Microcoleus sp, Aphanocapsa sp, Phormidium sp, Lyngbya
sp, Gleocapsa sp, Synechococcus sp, Spirulina sp with were set in compliance with their cellular
mechanism of nano silver creation, and were investigated by UV-VIS spectrophotometer, Energy-
dispersive X-ray (EDX) and scanning electron microscopy (SEM). All articles published by MDPI
are made immediately available worldwide under an open access license. No special. Figure 6
compares the ATR-FTIR spectra of teucrium apollinis extract and AgNPs functionalized by the same
plant extract. Feature papers are submitted upon individual invitation or recommendation by the
scientific editors and must receive. We hadn’t found anything similar that was discovered, and its
applications were completely unknown too. In addition, the contact area and interaction of the
nanoparticle with the medium are influenced by the nanoparticle’s size. The progress of the formation
of AgNPs can be observed through visual color changes or by using UV-Vis. I entered SUTD
wanting to graduate with a degree in material science, and the first thing I did was to seek for a
research opportunity in that field. The AgNP produced by this extract (see Figure 2 ), showed a
relatively large size in this study only after 10 weeks. The intensity of the plasmon resonance bands
was raised as time was increased, showing that large sizes are created after around 10 weeks, and
excluding any possibility of aggregation. Abdussalam-Mohammed W, Mohamed L, Abraheem MS,
Mansour MMA, Sherif AM. For instance, at higher pH values, smaller AgNPs were produced in
comparison to lower pH values. Among them, silver nanoparticles (AgNP) showed various specific
properties. The minimum inhibitory concentration (MIC) of AgNPs againstS. Figure 2 displays the
stability of AgNPs up to 10 weeks. The minimum inhibitory concentration (MIC) of AgNPs againstS.
Conflicts of Interest Authors have declared that there was no conflict of interest.