2. pairwise sequence alignment in pattern matching problem. 3. Illustrate nucleotide BLAST (BLASTN) Web Interface. 4. Compare BLASTN and BLASTP. 5. Compare PAM and BLOSUM 6. Compare local and global sequence alignment. 7. Difference between BLAST and FASTA. 8. Analyze dot-matrix methods, dynamic programming, and word methods of sequence alignment. 9. protein structure prediction in biological data and BALSA pairwise sequence alignment Tool 10. drug discovery and development processes and tools. 11. Difference between PAM and BLOSUM heuristic methods. 12. scoring matrices and dot plot method in sequence alignment. 13. Discuss smith-waterman and needle man dynamic programming algorithm 14. Analyze the algorithm for this form of local alignment and example. 15. List out the pattern matching applications in bioinformatics.