Name: Zaigham Syed

FORMATIVE: DNA Transcription

Provide the answers to the given tasks:

1. Arrange the given events of DNA transcription

in the correct order by assigning the
appropriate numbers to the spaces provided
before the letters.
3. RNA Polymerase matches RNA nucleotides with the
exposed DNA nucleotides
6. Non-coding Introns are removed to form a matured
mRNA strand
8. mRNA leaves the nucleus and enters the cytoplasm
2. RNA Polymerase binds to the DNA promoter where
transcription begins
4 . mRNA is made in the 5’-3’ direction
1 . DNA unzips to expose the DNA template
7 . mRNA molecule is released from the template
5 . Matching of RNA nucleotides to DNA template stops once RNA polymerase reaches the
termination point

2. In what location of a eukaryotic cell, does DNA transcription take place? Nucleus
3. What are two important roles of RNA polymerase?
1. binds to the DNA promoter where transcription is to begin and unzips the
gene that needs to be copied.
2. uses complementary base-pairing rules to match RNA nucleotides with the
exposed DNA nucleotides

4. In which direction of the DNA template do the RNA nucleotides form pairs with
their complementary counterparts? 3’ to 5’

5. What are the nitrogenous bases of the RNA nucleotides and their
complementary pairs?
adenine (pairs with thymine & uracil), cytosine (guanine), uracil (pairs with
adenine), guanine (pairs with cytosine), and thymine (pairs with adenine)
 6. Provide the correct RNA complementary pair of a DNA template:
TA C GCT AGT ACG ATT
AUG CGA UCA UGC UAA

7. When does the transcription stop? once the polymerase transcribes a sequence
of DNA known as a terminator.
8. When the matching ends, the unmatured mRNA is detached from the RNA
polymerase and DNA template. Why is mRNA during this stage called
unmatured?
it is because unmatured mRNA lacks necessary modifications such as adding a 5'
cap, a 3' poly(A) tail, and removal of introns through splicing, rendering it unable
to be effectively translated into protein.

9. What process removes the introns from mRNA? RNA splicing

10. What enzyme is involved in removing introns to form a matured mRNA? the
spliceosome
11. What is the product of DNA transcription? an RNA transcript.
12. Where will the matured mRNA go? exported from the nucleus to the cytosol
13. What is the use of mRNA? to potentially treat diseases and prevent infections.

Summarize the process: Provide important events happening during each stage of DNA
Transcription.

14. Initiation: the point of binding enzymes and proteins necessary for transcription
to promoter region in DNA. This is preceded by the unwinding of DNA.

15. Elongation: Complementary RNA nucleotides are added to the DNA template
strand by an enzyme called RNA polymerase. The enzyme slides along the DNA
template reading each nucleotide and building a complementary RNA molecule.

16. Termination: This is achieved when RNA polymerase binds to a particular

termination sequence in the DNA template. This series of events indicates to the
polymerase that it should leave the DNA and let go of its newly synthesized RNA
molecule.