EXPERIMENT 19

for normal organic constituents of

erform qualitative chemical analysis
oBJECf: To p
urine, th tion
S. R & Kale R. R "Biochemistry & Clinical Pathology'' 10 edi
ERENCE: Kale
REF N'rali publication
1004, I

fQUIREMENTS:
R wares: Test tube, holder
, beaker, glass rod, pipette etc.
d. h d ·d ,
Glass • t sod.1um ni·tropruss,.d e, so ,um y rox, e, picric acid
chemicals: Silver nitra e,
sodium carbonate.

Preparation of reagents:
tion in water.
1. silver nitrate solution: 5% w/v solu
2.sodium Nitr
oprusside solution:
tion in water.
3,Sodium hydroxide solution: 40% solu
tion
4. Picric acid solution: Saturated solu

PROCEDURE: INFEREMCE
TEST OBSERVATION
S. No.
acid
1. Schiffstest (for uric acid)
Black or yellow brown Uric
n form. present.
Moisten a strip of paper with stai
AgNO3 Soln + a drop of Urine.
is
2. Weyl's test (for creatinine)
Ruby red colour is Creatinine
ed and soon present.
Urine + drops of sodium form
nitroprusside + 10% NaOH. change to yellow.

3, A deep orange colour Creatinine is

!!ffe's test (for creatinine) present.
is formed.
Urine + saturated soln. of Picric
acid + anhydrous Na2CO3 mix
well by shaking.

I •f • •
I organic constituents of urine were
·
1at·1ve chem1cal ana ys1s or norma
RESULT·• Qual·t
Perfarmed.