NAME: GROUP:

SCHOOL: DATE:

1. Air holes found on tissue during trimming. B. Fixation

A. incomplete freezing C. Mounting
B. dirty wax is used D. Dehydration
C. there is wax in the metal mold
D. incomplete impregnation 10. Most important step in embedding.
A. orientation
2. sections of unequal thickness are produced. B. heated wax
A. the knife is dull C. heated metal mold
B. knife or block holder screw is loose D. specimen size
C. knife is dirty
D. all of the above

3. reasons to re-embed in filtered wax. FAULT REASON REMEDY

A. wax appears crystalline
ribbon is split or dirty embedding re-embed filtered
B. tissue is opaque
lengthwise vertical medium wax
C. both A and B
scratches are seen knife is dull sharpen the knife
D. A only
on sections
section cut is knife is not adjust the knife
4. remedy when sections are produced in
sometimes thin, clamped properly
unequal thickness
sometimes thick
A. sharpen the knife
a hole is formed in bubble of dirt re-embed freshly
B. reduce the tilt of the knife
the section formed in the wax if necessary
C. cool paraffin wax in ice
embedding
D. change the knife
medium
tissue is soft when incomplete repeat fixation
5. fault when the knife is blunt.
block is trimmed fixation
A. sections adhere and get broken against the
tissue is opaque insufficient repeat clearing, if
knife edge
clearing object
B. unequal thickness of ribbon
C. sections are squashed brittle or hard prolonged fixation
D. all of the above tissue
clearing agent incomplete repeat
6. Step after sectioning during actual practice turns milky dehydration dehydration with
A. de-paraffinize absolute alcohol
B. staining then clear again
C. xylene for 30 mins sections fail to horizontal surface re-adjust and re-
D. cool in the freezer form ribbons of the block is not orient the block
parallel to the
7. Used to clean microtome after sectioning. knife
A. Brush sections are knife edge is clean the knife
B. towel wrinkled, coated with edge
C. xylene compressed or paraffin
jammed

D. alcohol

8. ER, PR and HER2 NEU is:

A. Liver panel test
B. kidney panel test
C. gallbladder panel test
D. breast panel test

9. Most important step in tissue processing.

A. Labelling