SWEAT EXAMINATION

• Tested for the levels for Sodium & Chloride

• Used to diagnose cystic fibrosis
- a genetic disease that affects the mucous-secreting
glands of the body
**CFTR (Cystic fibrosis transmembrane conductance
regulatory gene)
-causes decreased epithelial cell chloride secretion
and excessive sodium reabsorption
•Risk Factor: Family history of cystic fibrosis
•Signs and Symptoms of Cystic fibrosis
1.Bulky offensive greasy stools (butter-like)
2.Noticeably salty sweat
3.Frequent respiratory infections and
chronic cough
4.Malnutrition
5.Male infertility (obstructive azoospermia)

MaLFreGIS
 PILOCARPINE IONTOPHORESIS
• At the test site, an electrode is placed over gauze containing
pilocarpine and electrolyte solution that will not interfere with
the sodium and chloride measurement.
• A second electrode (without pilocarpine) will be placed at
another site and a mild electrical current will draw the
pilocarpine into the skin where it stimulates the sweat glands.
 PILOCARPINE
IONTOPHORESIS
• The test site is carefully cleaned and dried, then a piece of pre-
weighed filter paper is placed over the test site and covered
with parafilm to prevent evaporation.
• Specialized collection devices may also be used.
• Sweat is collected for 30 minutes.
• The filter paper is retrieved and weighed to determine the
weight of sweat collected
 SWEAT EXAMINATION

• NV of Sweat Chloride: <50mmol/L

• Values >60mmol/L is indicative of cystic fibrosis
• Borderline: 50-60mmol/L

Tandaan mo lang dianne yung borderline 50-60 okayyyyyyyyyyy!!!

 METHODOLOGIES/LAB TESTS FOR
CHLORIDE
 Cotlove Chloridometer
 Microtitration with Mercuric Nitrate
 Cystic Fibrosis Analyzer
 Chloride Electrode Method