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Lineweaver-Burke Plot Analysis

The document contains Lineweaver-Burk plots showing the effect of an inhibitor on the velocity of an enzyme-catalyzed reaction at different substrate concentrations. Data tables provide reaction velocities with and without the inhibitor, which are then plotted as 1/velocity vs 1/substrate concentration to obtain linear plots for calculating kinetic parameters.

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TAN YEE LING
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218 views2 pages

Lineweaver-Burke Plot Analysis

The document contains Lineweaver-Burk plots showing the effect of an inhibitor on the velocity of an enzyme-catalyzed reaction at different substrate concentrations. Data tables provide reaction velocities with and without the inhibitor, which are then plotted as 1/velocity vs 1/substrate concentration to obtain linear plots for calculating kinetic parameters.

Uploaded by

TAN YEE LING
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd
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1/ [S] 1/V

0.666667 4.761905
0.5 4
0.333333 3.571429
0.25 3.030303
0.125 2.272727
0.0625 2.5

[S] V Lineweaver-Burke plo


1.5 0.21 5
2 0.25 4.5 f(x) = 4.04919355706431 x + 2.04850851992526
3 0.28

1/ Velocity (min/µmole)
4
4 0.33 3.5
8 0.44 3
16 0.4 2.5
2
1.5
1
0.5
0
0 0.1 0.2 0.3 0.4

1/ [Sucrose] (1/mM)

without inhibitor with inhibitor


[S] V [S] V
1.5 0.21 1.5 0.08 Lineweaver-Burke plo
2 0.25 2 0.1 14
3 0.28 3 0.12
12
1/ Velocity (min/µmole)

4 0.33 4 0.13 f(x) = 11.109128375077 x + 4.80121005908081


8 0.44 8 0.16 10
16 0.4 16 0.18
8

1/ [S] 1/V 1/ [S] 1/V 6


0.666667 4.761905 0.666667 12.5
4 f(x) = 4.04919355706431 x + 2.04850851992526
0.5 4 0.5 10
0.333333 3.571429 0.333333 8.333333 2
0.25 3.030303 0.25 7.692308
0
0.125 2.272727 0.125 6.25 0 0.1 0.2 0.3 0.4 0.5
1/ [Sucrose] (1/mM)
0.0625 2.5 0.0625 5.555556
Lineweaver-Burke plot

9355706431 x + 2.04850851992526

0.2 0.3 0.4 0.5 0.6 0.7

1/ [Sucrose] (1/mM)

Lineweaver-Burke plot

x + 4.80121005908081

1 x + 2.04850851992526
without
inhibitor

0.3 0.4 0.5 0.6 0.7


1/ [Sucrose] (1/mM)

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