ANUSHKA PAI

2022A4PS0136G
SECTION NO – P15

PROTEIN – INSULIN
ORGANISMS –
Homo Sapiens
Cavpo (guinea pig)

AMINO ACID SEQUENCES

HUMAN
>sp|P14735|IDE_HUMAN Insulin-degrading enzyme OS=Homo sapiens OX=9606 GN=IDE PE=1 SV=4

MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPED

KREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKK

YPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKD

REVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQE

LLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQ

LYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLV

GGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAV

AFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAI

VSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPL

EKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELL

KDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFE

IIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLL

SRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQ

QRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRA

NGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKL

SAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLA

REMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL
 GUINEA PIG
>sp|P01329|INS_CAVPO Insulin OS=Cavia porcellus OX=10141 GN=INS PE=1 SV=2

MALWMHLLTVLALLALWGPNTGQAFVSRHLCGSNLVETLYSVCQDDGFFYIPKDRRELED

PQVEQTELGMGLGAGGLQPLALEMALQKRGIVDQCCTGTCTRHQLQSYCN

HUMAN INSULIN

PEPSTATS
PEPINFO HISTOGRAM
PEPINFO HYDROPATH
 CAVPO (GUINEA PIG) INSULIN

PEPSTATS
PEPINFO HISTOGRAM
PEPINFO HYDROPATHY
 SEQUENCE ALLIGNMENT

LOCAL ALLIGNMENT (SMITH WATERMAN)

GLOBAL ALLIGNMENT (NEEDLEMAN-WUNCH)

########################################
# Program: needle
# Rundate: Fri 13 Jan 2023 04:30:16
# Commandline: needle
# -auto
# -stdout
# -asequence emboss_needle-I20230113-043014-0356-70093097-p2m.asequence
# -bsequence emboss_needle-I20230113-043014-0356-70093097-p2m.bsequence
# -datafile EBLOSUM62
# -gapopen 10.0
# -gapextend 0.5
# -endopen 10.0
# -endextend 0.5
# -aformat3 srspair
# -sprotein1
# -sprotein2
# Align_format: srspair
# Report_file: stdout
########################################

#=======================================
#
# Aligned_sequences: 2
# 1: IDE_HUMAN
# 2: INS_CAVPO
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 1029
# Identity: 32/1029 ( 3.1%)
# Similarity: 51/1029 ( 5.0%)
# Gaps: 929/1029 (90.3%)
# Score: 17.0
#
#
#=======================================

IDE_HUMAN 1 MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRI 50

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 51 GNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDP 100

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 101 PNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYY 150

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 151 FDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWR 200

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 201 LFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYS 250

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 251 SNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQ 300

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 301 LYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSEL 350

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 351 KSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQK 400

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 401 LRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVL 450

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 451 TAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQY 500

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 501 KQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPAL 550

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 551 IKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELL 600

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 601 KDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMA 650

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 651 TFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDEL 700

INS_CAVPO 0 -------------------------------------------------- 0

IDE_HUMAN 701 KEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLI 750

.|....||:.|.:.||...| |.||.:
INS_CAVPO 1 ------------MALWMHLLTVLALLALWGPN-TGQAFV----------- 26

IDE_HUMAN 751 EHAHTKPLLPSQLVRYREVQLPDRGWF-VYQQRNEVHNNCGIEIYYQTDM 799

::.|..|.||........|.|:| :.:.|.|:.:. .:| ||::
INS_CAVPO 27 ----SRHLCGSNLVETLYSVCQDDGFFYIPKDRRELEDP-QVE---QTEL 68

IDE_HUMAN 800 -----QSTSENMFLELFCQ---IISEPCFNTLRTKEQL-GYIVFSGPRRA 840

....:.:.||:..| |:.:.|..|. |:.|| .|..
INS_CAVPO 69 GMGLGAGGLQPLALEMALQKRGIVDQCCTGTC-TRHQLQSYCN------- 110

IDE_HUMAN 841 NGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALA 890

INS_CAVPO 110 -------------------------------------------------- 110

IDE_HUMAN 891 IRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFY 940

INS_CAVPO 110 -------------------------------------------------- 110

IDE_HUMAN 941 KEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPE 990

INS_CAVPO 110 -------------------------------------------------- 110

IDE_HUMAN 991 VIQNMTEFKRGLPLFPLVKPHINFMAAKL 1019

INS_CAVPO 110 ----------------------------- 110

#---------------------------------------
#---------------------------------------

TRANS SEQ

#---------------------------------------
# Similarity: 26/87 (29.9%)
# Gaps: 43/87 (49.4%)
# Score: 36.5
#
#
#=======================================

IDE_HUMAN 563 QDDKFF-LPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAA

611
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