4/7/24, 11:16 PM Sabouraud agar - Wikipedia

Sabouraud agar
Sabouraud agar or Sabouraud dextrose agar (SDA) is a
type of agar growth medium containing peptones.[1] It is used to
cultivate dermatophytes and other types of fungi, and can also
grow filamentous bacteria such as Nocardia.[2][3][4] It has utility
for research and clinical care.

It was created by, and is named after, Raymond Sabouraud in

1892. In 1977 the formulation was adjusted by Chester W.
Emmons when the pH level was brought closer to the neutral Bottom view of a Sabouraud agar
range and the dextrose concentration lowered to support the plate with a colony of Trichophyton
growth of other microorganisms. The acidic pH (5.6) of traditional rubrum var. rodhaini
Sabouraud agar inhibits bacterial growth.[5] Peptones are complex
digests and can be a source of variability in Sabouraud agar.[6]

Typical composition
Sabouraud agar is commercially available and typically
contains:[7]

40 g/L dextrose
10 g/L peptone
Sporothrix schenckii in Sabouraud
20 g/L agar agar
pH 5.6

Medical use
Clinical laboratories can use this growth medium to diagnose and
further speciate fungal infections, allowing medical professionals
to provide appropriate treatment with antifungal medications.
Histoplasma and other fungal causes of atypical pneumonia can
be grown on this medium. Sabouraud agar used in combination
with additional media, such as Inhibitory Mold Agar (IMA), Candida albicans in Sabouraud agar
improves identification of fungal clinical isolates.[8]

References
1. "Omnipresence of Microorganisms in the Environment" (https://web.archive.org/web/20081006012
923/http://waksmanfoundation.org/labs/lsu/omni.html). Waksman Foundation For Microbiology.
Archived from the original (http://www.waksmanfoundation.org/labs/lsu/omni.html) on 2008-10-06.
Retrieved 2008-10-24.
2. Sandven P, Lassen J (November 1999). "Importance of selective media for recovery of yeasts
from clinical specimens" (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC85742). Journal of
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4/7/24, 11:16 PM Sabouraud agar - Wikipedia

Clinical Microbiology. 37 (11): 3731–3732.

doi:10.1128/JCM.37.11.3731-3732.1999 (https://doi.org/10.112
8%2FJCM.37.11.3731-3732.1999). PMC 85742 (https://www.n
cbi.nlm.nih.gov/pmc/articles/PMC85742). PMID 10523586 (htt
ps://pubmed.ncbi.nlm.nih.gov/10523586).
3. Guinea J, Peláez T, Alcalá L, Bouza E (December 2005).
"Evaluation of Czapeck agar and Sabouraud dextrose agar for
the culture of airborne Aspergillus conidia". Diagnostic
Microbiology and Infectious Disease. 53 (4): 333–334.
doi:10.1016/j.diagmicrobio.2005.07.002 (https://doi.org/10.101 Trichophyton terrestre in Sabouraud
6%2Fj.diagmicrobio.2005.07.002). PMID 16263232 (https://pu agar
bmed.ncbi.nlm.nih.gov/16263232).
4. "About Modified Sabouraud Agar" (https://web.archive.org/we
b/20090419083512/http://www.bd.com/ds/technicalCenter/inserts/Sabouraud_Agar_Modified.pdf)
(PDF). www.bd.com. Archived from the original (http://www.bd.com/ds/technicalCenter/inserts/Sab
ouraud_Agar_Modified.pdf) (PDF) on 19 April 2009.
5. Hare JM (9 December 2012). "15. Sabouraud agar for fungal growth" (https://books.google.com/b
ooks?id=IQOwjVXwEQIC&pg=PA212). In Gupta VK, Tuohy MG, Ayyachamy M, Turner KM,
O'Donovan A (eds.). Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology.
Springer. p. 212. ISBN 978-1-4614-2355-3.
6. Odds FC (January 1991). "Sabouraud('s) agar". Journal of Medical and Veterinary Mycology. 29
(6): 355–359. doi:10.1080/02681219180000581 (https://doi.org/10.1080%2F0268121918000058
1). PMID 1815027 (https://pubmed.ncbi.nlm.nih.gov/1815027).
7. "Recipes" (https://web.archive.org/web/20121214202207/http://bugs.bio.usyd.edu.au/learning/res
ources/Mycology/Growth_Dev/recipes.shtml). University of Sydney. 2004. Archived from the
original (http://bugs.bio.usyd.edu.au/learning/resources/Mycology/Growth_Dev/recipes.shtml#sab
ouraud) on 14 December 2012.
8. Scognamiglio T, Zinchuk R, Gumpeni P, Larone DH (May 2010). "Comparison of inhibitory mold
agar to Sabouraud dextrose agar as a primary medium for isolation of fungi" (https://www.ncbi.nl
m.nih.gov/pmc/articles/PMC2863941). Journal of Clinical Microbiology. 48 (5): 1924–1925.
doi:10.1128/JCM.01814-09 (https://doi.org/10.1128%2FJCM.01814-09). PMC 2863941 (https://ww
w.ncbi.nlm.nih.gov/pmc/articles/PMC2863941). PMID 20220162 (https://pubmed.ncbi.nlm.nih.gov/
20220162).

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