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Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster
Bean
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Nirmal Singh
Anurag Malik
Himani Punia
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Contents
vi
12. Varietal and Hybrid Seed Production in Solanaceous Vegetables 199-226
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Himangini, Neha Thakur, Anurag Malik, and Nirmal Singh
vii
CHAPTER 8:
SEED PRODUCTION TECHNOLOGY OF PEAS AND BEANS:
FRENCH BEAN, PEA AND CLUSTER BEAN
Neha Thakur Uppuluri Tejaswini
Assistant Professor, Department of seed science and technology,
Department of Seed Science and Technology, Gandhi Krishi Vigyana Kendra (GKVK),,
College of Horticulture and Forestry, Bengaluru, Karnataka
Thunag, Mandi, Himachal Pradesh - 175048 (tejaswini.uppulurichanti@gmail.com)
(nthakur0708@gmail.com)
1. Introduction:
French bean (Phaseolus vulgaris L.) also called as snap bean, string bean, fresh beans or
garden beans is a member of Leguminosae family. It is supposed to be originated in the New World
as well as in Mesoamerica region and domesticated 5000 B.C. It is cultivated on an area of 227.78
thousand ha with production of 2276.95 thousand metric tonnes green beans in India [1]. It is majorly
cultivated as Kharif season crop. The genus Phaseolus contains 80 cultivated and wild species from
the New World out of which five are domesticated species: P. lunatus (Lima, butter or sieva bean), P.
vulgaris (common bean), P. acutifolius (tepary bean), P. coccineus (runner or kidney bean) and P.
dumosus (acatalete, botil or year bean) [2] Table 1. P. coccineus or runner or kidney bean is resistant
to bacterial blight, cold tolerant, root rot resistant, and also bean yellow mosaic virus. Beans are
consumed as green bean as well as dry seed form that is a rich source of protein, important minerals,
dietary fiber, vitamin B6, folic acid, thiamin, riboflavin, niacin and minerals including potassium,
zinc, copper, iron, calcium, and magnesium. Globally a large population of vegetarians consumes
Phaseolus as pulse called as Rajmash in India. This bean as a pulse is rich in dietary protein which
complements the lacking amino acids lacking in cereals diets [2]. It also constitutes some
antinutritional factors viz, raffinose, trypsin inhibitor, hemagglutinin, tannin and phytic acid. These
endogenous toxin components can cause various neurological symptoms such as limb numbness,
headache, chest tightness, and even severe coma, multiple organ damage or sudden death [3].
The protein content of common bean varies from 19.70 to 24.30 % [4]. It is a rich source of
Fat (0.1g), Fiber (1.8g), Protein (1.7g) and Carbohydrate (4.5g) per 100 grams of green pod
respectively [5]. It also supplements Thiamine, Riboflavin, Vitamin C and Vitamin A. According to
third advanced estimate beans occupy an area of 228 thousand hectares with production of 2257
thousand metric tonnes in the year 2018-19 [6]. The common bean is diploid in nature (Table 1).
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
Polyploidy can be achieved in common/ French bean by treatment with 0.25% colchicine solution [7].
But this treatment can result in generation of tetraploids that are deformed with thick and dark green
leaves, change in pollen grains and reduced fertility level.
Garden pea (Pisum sativum L.) is another edible legume and member of Leguminosae family.
Its name is derived from English word peasse [8]. It is originated in Central Asia, Near East,
Abyssinia and the Mediterranean Sea [9]. Pea is one of the oldest cultivated plants and was popular
for cultivation in neoithic farming villages of Near East around 7000 B.C. A productive crop of pea
can catch approximately 300 kg Nitrogen in one hectare through above ground biomass and 70% of
this amount in its seed. Therefore pea is a key plant in sustainable agriculture because of its high
production potential [10]. It is also called as green pea which is cultivated for its culinary purpose as
green pods in India for preparation of soups and frozen canned food. Straw of this green pea is
nutritious so it is used as animal fodder. Apart from this there are many health benefits like reducing
weight, decreasing the blood pressure level, arthritis, osteoporosis, improvise the immune system,
anti-ageing, improves the digestion and prevention of stomach cancer. Pea are recorded to be grown
over an area of 551 thousand hectares with production of 5533 thousand metric hectares in 2018-19
[6]. This is rich in carbohydrates, proteins and vitamin A and C, Calcium and phosphorus. Nitrogen
fixation is done by Rhizobium leguminosarum, which recognizes as a soil building crop.
Garden pea is one such important vegetable crop, serve higher protein to the people where the
non-availability of non-vegetarian and who don’t like meat and its products. Another species under
Pisum genus is P. arvense (field pea) [11]. Other wild relatives of pea are P. fulvum, P.
sativum subsp. Elatius these species are characterized by sudden and unique dehiscence of pods and
ballistic spread of seeds [12]. Grown all over the country with suitable crop varieties and crop rotation
with this crop as it helps in synergistic effect on cereal crops. Use of pure lines as it is pre-dominantly
self-pollinated crop. Male sterile lines are by the mutagenesis, but for production of hybrids is not
adopted as it has the low significant vigour as there is difficulty in crossing. So, use of disease-free
stocks for planting is necessarily used in the hybrid seed production plot. Pea plant is associated with
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
Gregor Johann Mendal who is known for theories of genetics based on his experiments on this plant.
He selected the pea plant for his experiments because of the following features:
• The flower is bisexual and hermaphrodite. As it is a self-pollinated and fertilized crop, where
the petals are enclosed both the reproductive organs. Even though cross pollination and
fertilization can be achieved.
• Annual growing plant and is easy to cultivate. The characters are inheritable and desirable
characters are seen.
Several experiments made for inter generic hybridization with pea were not of much success.
In one such crossing of Vicia faba with pea poor seed set was observed [11] after development of
hybrid embryos just for six days.
Gaur bean is a drought resilient crop and has occupied a special place on commercial scale as
a high valued crop due to its gum producing capacity. About 30-33% gum is present in the endosperm
of its seed along with protein 18% and fiber 32%. In 1948, cluster bean starts gaining its popularity as
an industrial crop after the exploration of gummy substance named galactomannan in the endosperm
of its seed [14]. This Gum is a hydrocolloid that occurs naturally in seed`s endosperm also called as
Guaran. After dehusking, the seed endosperm is grounded and gum is being extracted [15]. Gum
attained from cluster bean seeds is a choice for food, cosmetics, oil, textile, mining, pharmaceutical
industries and agrochemical in paper etc. across the world [16]. It is also used as ice cream stiffner,
puddings, whipped cream. The galactomannan gum forms a viscous gel when mixed in cold water
(17). Pods of the crop can also be used for vegetable purposes whereas; straw and seed being high in
nutritional value are good for fodder and feed for the livestock. It can also be utilized to grow as a
cover crop and green manure to increase soil fertility [14].
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
The crops belonging to family Leguminosae; French beans are highly self-pollinated with
perfect legume shape flowers with zygomorphic symmetry. The floral structure arrangement in keel
(inner most two petals fused to form boat shaped structure), two wing petals, and a standard closely
cover the essential organs the pistil and stamen. Two wings are smaller in size than standard. Third
whorl is androecium, which consists of 10 stamens with diadelphous condition (Figure 2). It has long
stalk called filament with anther consists of pollen grains. The lower portion of filament is fused to
form a stamina tube which is equal to the length of ovary. However, the is a gap in stamina tube to
free the 10th stamen. Gynoecium is the fourth whorl of a flower, consisting of ovary style and stigma
in the centre of the flower. The style has a sticky feathery stigma at the end to attach the pollen which
further converted into a fruit. This arrangement prevents any foreign pollen to enter the flower hence,
there is no chance of cross contamination. That is the reason that few hybrids are found in this crop.
Anthesis begins at topmost axil of leaf in mainstem (for determinate varieties) or on variable axillary
nodes (for indeterminate varieties) (18) It starts at 6th or 7th node indeterminate bush type (19). Single
pistil can consist of about 9 to 10 ovules. The characteristic feature of Phaseolus genus is coiled
appearance of the keel with one to two turns. Another trait specific to this genus is presence of
uncinate hairs on both vegetative and reproductive structures of the plant (20). Pollen grains
(diadelphous) are arranged in proximity of stigma that pollens are directly shed onto stigma. Stigma
remains receptive up to from two days before anthesis to one day after it and it coincides with pollen
viability.
Peas and beans highly self-pollinated crop prominently due to cleistogamous nature with less
than one per cent chances of natural crossing. First flower is produced at 5th to 11th node in early
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
varieties and at 13th to 15th node for late varieties (8). It takes 8 to 12 hour and 24 to 28 hours after
pollination for pea pollen to germinate and effect fertilization respectively (19). Beans crop reaches
green tender stage 25 days after pollination. A drop of 60 to 70 per cent are observed in flowers and
pods formed at later stage of plant growth. In pea flowering starts from 40 to 50 days after planting.
Flowers are arranged in axillary raceme (one to three flower per raceme) and P. sativum flowers are
white coloured whereas for P. arvensis it is of purple, lavender or red in colour. The unequal lobes in
flower called as calyx, protect the bud stage of flower. Flower structure is typical legume as described
in above section. Anthesis starts acropetal in fashion i.e., lower node flowers first and there may be a
difference of five to six days for anthesis in between nodes (21). In a young flower the filament is
shorter which elongates at maturity stage or at the time of anther dehiscence to reach up to the cap of
keel so as to cover up the style (22). Up to 13 ovules are borne alternatively and hooked to two
placentas. Pollen remains viable for several days after anther dehiscence and stigma is receptive from
several days before anthesis till one day after flower opening.
Cluster bean plant is an herbaceous, erect, annual legume (Figure 1) with an indeterminate
growth habit reaching up to a height of 0.5 mt to 3 mt and the total crop cycle of the plant vary
between 80 to 160 days [23]. It has a well-developed deep taproot system along with the lateral roots
and the entire roots are found with the light-coloured nodulations. Colour of the nodules depicts its
effectiveness viz; white or brown colour nodules are completely ineffective whereas effective nodules
are light pink in colour [23]. Plant has robust, single, unbranched stem. It is hollow or ribbed/angled
with mild to slight pubescence on it [23]. Its’ leaves are trifoliate, alternate and ovate in shape. Leaf
Margins are serrate. Petioles are long with a prominent pulvinus at base of the leaf for easy movement
of the leaves. Leaves are 5-10 cm in length with terminal leaf slightly larger than the others.
The inflorescence of cluster bean plant is axillary raceme (Figure 2). Length of the raceme
varies between 8-10 cm. Flowers are present in clusters thus justifying its name as cluster bean [24].
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
Flower is of cleistogamous in nature which makes it a strictly self-pollinated flower. They are small
white or purple in colour borne on the axial of the raceme. They are small, complete and
hermaphrodite with papilionaceous corolla that has orbicular oblong petals, 5 unequal teeth type
linear sepals from the calyx and long broad keel petals. Anthesis of flowers occur at during morning
hours i.e., 6:00 am – 10: 00 am. Diadalphous stamens are 10 in number which gets dehisce to
extricate pollen grains upon maturity i.e., 10 pm – 1 pm during the day. Stigma is short in length [25].
Fruits are erect, beaked, oblong, clustered and compressed with ridges on the sides of the pods
(Figure 3). Pods are glabrous and fleshy up to 5-10 cm in length possessing 6-11 oval shaped, white
to grey or black seeds of various shapes and colour [26].
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
Pea is cultivated on the broad varieties of soils. Pea grows well where the soil is well drained
with the pH range of 6 to 8. To get good yield and quality seed, soil should contain good organic
matter. Farm yard manure which is best source of organic matter is applied at the time of land
preparation. Soil test should be done to know the deficiency (as discussed in nutrient management
section) in the soil. Climate plays a vital role for sowing and place of crop rotation in the season. The
peas come up best in cold and moist condition as it is a cool season crop. Ideal temperature for
cultivating peas is 10 to 30 °C and best suited at temperature of 7 to 24 o C for better plant growth and
o
for the best yield 13 to 20 C [29]. Higher temperatures during flowering leads to abortion and
reduced seed set. Also, above the maximum temperature it effects the yield and quality. The most
important is presence of sufficient soil moisture content during critical stages of crop. Rainfall
requirement of peas are 500mm.
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
Cluster bean crop performs well on fertile, light and fine textured, well drained sandy soils
with soil pH of 7.0 to 8.5. Deep black heavy soil with high soil salinity and waterlogged conditions
significantly reduces nodulation in plants thus, reduces the number of pods formed as a result of
which seed yield also decreases [30]. It is a photosensitive crop that requires certain climatic
conditions for proper growth and development. Cluster bean plant is properly acclimatized to desert
environment with semi-arid climatic conditions thus can tolerate high temperature up to 40ºC and dry
conditions. An ideal temperature range lies between 30-35°C for proper germination of seeds [31],
whereas for vegetative growth 32-38°C is considered as an optimum temperature range coupled with
long day conditions. An annual rainfall of 25 to 30 inches is sufficient for proper plant growth (17).
Further, for flowering and formation of fruits/pods short day conditions are considered good. Plant is
resilient to shade but cannot withstand frost conditions. Such idealistic atmospheric conditions favour
endosperm development and hence, as a result enhance the gum content of the seeds.
In pea time of sowing critically depends on climate of the area. Usually, pea is sown in
second fortnight of November to get higher yields. To achieve higher seed production some practices
can be followed such as a deep ploughing in summers followed after every three years; treating seeds
before sowing, soil testing, variety selection and integrated approach for plant nutrient and pest
management [32]. Cluster bean can be cultivated on wide soil conditions and preferred type is fertile,
medium textured. It’s a versatile crop due to tolerance to drought. The plant stops growing and
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
resumes its growth only when sufficient moisture is available [17]. Sowing of seeds of cluster bean
should be done by 15th of July under non irrigated conditions whereas, under irrigated conditions
sowing can be extend to the end of July also but there may be a loss in seed yield is sowing is
extended beyond.[13]. If at the time of maturity there is heavy downpour then physical quality of seed
is reduced with more of black and shriveled seeds. So only dry weather is preferred at harvesting of
crop for seeds [17] and a green manure crop instead of seed crop is preferred where the humidity
level is high. This bean is an excellent soil improving crop as it is a deep footed legume. After seed
harvesting gaur bean plant residue can be turned into dry organic matter for soil as well as used as
mulch.
Leguminous crops are treated before sowing with two grams of Captan (fungicide) for one kg
seed followed by coating seeds in a bacterial culture of Rhizobium phaseoli.
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
In pea crop Rhizobium leguminosarum @2 ml per planting hole can enhances pea seed
production. This treatment has a positive effect on the fresh and dry weight of seed as well as filling
percentage of seed. It can reduce seed infection severity by inducing several proteins and metabolites
involved in pathogen response. Rhizobium culture packet is often formed slurry with half litre of
water to coat the seed. This treatment should be done on the seeds that are treated with fungicides
e.g., Bavistin @1g or Captan @ 2g per kg seed. The sequence of seed treatment should always be
Fungicide- Insecticide- Rhizobium. If this sequence is reversed then the microorganism may not be
effective in the soil.
8. Improved Varieties:
Various universities and institutes of ICAR are involved in developing a number of improved
varieties in our country. Several varieties have been developed that are different in maturation days,
quality, plant stature and production of seeds for planting in various dry and semi-arid, tropical and
semi temperate regions. Following section enlists some of the important varieties of beans and peas
along with description.
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
8.1.3. IPL220:
It is cultivated in Assam, Bihar, West Bengal and some parts of Uttar Pradesh with a yield of
14-16 q/ha. The seeds of this variety are biofortified with about 114 milligrams per kilograms of Iron
and 63 milligrams per kilograms of Zinc. It is also resistant to rust, Fusarium wilt and Stemphylium
blight diseases.
8.2. PEA:
Few important varieties of pea are Bonneville, Jawahar, Arket, Early Bejar etc.
• Early varieties - Arkel, Jawahar matar 4, Pant matar 4 etc
• Mid-season varieties - Bonneville, Jawahar matar 1, jawagar matar 2 etc.
• Late season varieties- Vivek -6, Sylvia etc.
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
average yield of variety is 110-120 q/ha and 48-49 selling percentage. There is resistance to powdery
mildew.
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
9. Nutrient Management:
French bean crop is a complete crop in itself due to well deep well root system and plant for.
For nutrient management it requires 30 FYM and NPK @ 60:120:50 per hectares at the time of field
management. Fertilizer is applied in split dose with fifty per cent of nitrogen fertilizer + complete
amount of phosphorus and potassium broadcasted or put in beds on seed sowing. The remnant fifty
per cent nitrogen is put one month after sowing. Under integrated nutrient management an
application of 75% recommended dose of fertilizer and 25% vermicompost + lime application
generates a seed yield which is efficiently more and marketable for Rabi season crop of French bean
[37]. To get good yield the manure and fertilizers are to be supplied with sufficient quantity. For pea
crop about 2 tonnes per hectare of Farm yard manure is applied to soil during the preparation of land.
Based on soil test results the quantity of 30 kg of nitrogen, 45 kg of phosphorus and 50 kg of
potassium. Half of the nitrogen with full phosphorus and potassium at the time of sowing. Remaining
nitrogen is given after 30 days of sowing. Marsh spot is a major disorder caused by mangamese
deficiency in pea which can be cured by pre sowing basal application of solid Manganese sulphate @
40 kg ha-1. This deficiency is showing as brown empty centers in immature seeds [19].
In case of cluster bean, the recommended nutritional requirement of cluster bean is 50:60:60
kg NPK per hectare. At the time of sowing half dosage of nitrogen fertilizer and entire quantity of
phosphorus as well as potash fertilizer is broadcasted into earth and remaining half dose of nitrogen
should be applied after 25-30 days of first dosage [33]. It is prudent to practice integration of both
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
organic and inorganic nutrients for the crop for which 25 tons of farm yard manure (FYM) must be
applied 15 days prior to sowing of the crop. While using inorganic fertilizer it should be made sure to
place it at five centimeters depth beneath the seed. Organic compost enhances the moisture retention
property of soil thus providing nutrients essential for the crop establishment and growth.
10. Irrigation:
Cluster bean is a rainfed crop of arid and semi-arid regions. If water is available for irrigation,
it should be applied whenever crop goes under moisture stress mainly at critical stages of crop
development i.e., at flowering stage and seed formation stage. It is recommended to prepare proper
bunding in the field as the crop is grown in arid and semi-arid regions efficient management of water
is of major concern. Adequate mulching practices should be followed to conserve the moisture and
minimize moisture loss from the soil due to high water evaporation. Irrigation should be applied 2-3
days prior to sowing the crop. Light irrigation should be applied after 6-7 days after sowing for better
germination of the seed. If adequate irrigation facilities are available a minimum 5 irrigation should
be given to the crop regularly at 15 days interval. Field should be properly levelled with proper
drainage so that water logging condition should not arise in the field at any stage of crop
development.
Pea requires a seed rate of 120 to 150 kg ha-1 and 60 to 75 kg ha-1 for early and mid-late
cultivars respectively [19]. It can be sown at a spacing of 30 cm × 7.5 cm and 60 cm × 7.5 cm spacing
in early and mid-late cultivars respectively. A seed rate of 75 Kg ha-1 and 30 Kg ha-1 is needed for
bush type and climbing type cultivars of beans respectively. Former cultivar’s seeds can be sown at a
spacing of 45 cm × 15 cm and later one at 90 cm × 15 cm respectively. Before sowing seed should be
treated with Rhizobium sp. to get greater yields and quality seed. Seeds of pea should be soaked in
water one day before, to enhance the physiological activity of seeds to get good germination. Seeds
were sown either by broadcasting or dibbling. In the seed production plot, it is very much important.
Spacing depends on the growing habit of the crop. Spacing is used for walking for roguing, weeding,
identifying of off types, spraying chemicals, collecting pollens, dusting pollen on female flowers and
for harvesting. Spacing required by this crop is 45 ×25 cm. Flat bed layout should be there.
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
Quantity of seed recommended for sowing one hectare of land of cluster bean is 15-20 kg for
one growing season [38] and to avert the seeds from various soil borne pathogens seeds are treated
with Carbendazim @ 1 gram + Thiram@ 2 grams for one Kg of seed succeeded by inoculation with
suitable Rhizobium culture @ 600g/12-15 kg seed. Seeds after the treatment should be sown at 2 cm
depth in a priorly irrigated field [39]. Majority of farmers have been found sowing the crop through
broadcast method which not only makes the inter culture practices difficult but also compromises
with the quality and quantity of the end product. Therefore, to sustain ideal population of the plants,
improved germination percentage and for easy inter cultural practices seeds should be sown in plant
to row system. Cluster bean branched varieties should be sown at row to row spacing of 45 × 60 cm
and a distance of 10 to 15 cm in between plants [40] [55]. Cultivator or seed drill can also be used for
sowing. Further a distance of 30 cm can be kept in between rows and 10-15 cm in between plants for
single stem variety.
The kharif season cluster bean suffers from extensive weed competition mainly by grassy,
sedged and broad-leaved weeds thus, reduces the crop yield. Weeds compete with the crop for
nutrient, moisture and space. Therefore, to avoid such competition and obtain a good yield from the
crop needs to be devoid of weeds for 30 - 45 days after sowing [41]. Hence, two hand weeding viz;
first after 20-25 days of sowing and second 40-45 days after sowing is sufficient to obtain a good crop
yield. An alternate to manual weeding is herbicides. A as pre-emergence spay of pendimethalin @
0.75 kg/ha a.i. and post emergence application of Imazehtapyr 40g/ha a.i. in 600 lit of water after 20-
25 days of sowing should be given to control the weeds [30].
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
Table 3: Isolation requirement for French bean, pea and cluster bean [42].
14. Irrigation:
Depending upon the moisture sensitivity of crop as discussed in section ‘cultural practices’
frequent and light irrigation is practiced in bean crop instead of flood irrigation. For French bean
cultivation the irrigation schedule is as discussed; sowing crop in well moist soil; first irrigation at 15
to 20 days after sowing; then frequent irrigation at weekly interval. Shortage or excess of moisture
can cause deformity in pods [28]. The number or frequency of irrigation depends on the season of
cultivation. In pea crop irrigation should be provided in 9-10 days interval. Legume crops require
more water compared to cereals, where this pea is provided irrigation at the stage of flowering, pod
development. Water logging should be avoided, if water is logged it should be drained well.
Inspection of a crop stand at regular intervals is an essential step to authenticate the purity
percentage of the crop for specific certification standards. A thorough inspection of the crop on
various growth and development stages should be conducted on regular basis. Inspection should be
done by well trained and qualified officials [43]. In cluster bean minimum two field inspection should
be done by field inspector. First before commencement of flowering to actuate the field certification
standards like isolation distance, volunteer plants if any and planting ratios, second inspection is done
at the time of flowering and fruit set to rough out off-types (Table 4) and diseased plants [34] affected
by bacterial blight and anthracnose and pollen shedders in female plants if any [30].
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
Table 4: Off types requirement for French bean, pea and cluster bean [42]
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
flower drop and plant death. Preventive measure of crop rotation with non host crop e.g. corn, wheat
can be done. Along with wide row spacing, excessive use of N fertilized should be avoided.
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
days, if necessary; iii) Avoid late planting; iv) After harvest collect the plants left in the field and burn
them
16.2.1.6. Rust:
It appear as yellow spots in round and elongated clusters and consecutively appearance of
powdery and light brown colour on tissue. Infected stem turns out malformed and the affected plant
dies out. Spray of Mancozeb 75 WP @ 2 g / liter of water can prevent disease spread. The affected
plants residue should be discarded and burnt.
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
can be introduced in the field [44]. Spraying of 1.25 liter of Dimethoate 30 EC per 1000 liter of water
per hectare can be used as chemical spray. It can berepeated at an interval of 10-12 days.
16.3.1.3. Anthracnose:
This disease is caused by Colletotrichum capsici f. cyamopsicola. Black spots appear on the
petiole, leaves and stem. Its management is done by applying Zineb spray @ 2 kg in 500 L of water
per hectare to control the disease.
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
kg ha-1 or dust of sulphur powder @ 20-25 kg ha-1 or spray dinocap @1.5 ml L-1 of water can be used
to manage the crop.
17. Harvesting:
Seed crop is harvested at the harvestable maturity as compared to physiological maturity in
case of horticultural crops. It takes a month for pea crop to reach harvestable maturity after attaining
green pea stage [19]. Beans crop attain maturity at a range of 90 days to 120 days after sowing
depending upon on variety. Highest storability of seed and maximum germination and vigour in bean
seed can be achieved by harvesting it at 90% of brown colour pod stage and storing in cool room 16
month [45]. In pea crop also care is taken to harvest as it should reach the physiological maturity
once the outer pod has turned green to yellow colour. Entire plant is picked and taken to the threshing
yard. Moisture content <13% is optimum, if it is <20% it can be dried to optimum moisture content.
If the moisture content reached <9-10% which is less than optimum extra care taken to avoid
mechanical damage. The seed can be stored for1-2 years at ambient temperature and relative humidity
(RH). If it has to be stored for > 2 years at lower RH without the loss of viability in the seeds.
Cluster bean crop is harvested for seed purposes should be critically monitored at maturity
stage. Gaur bean pods do not shatter upon maturity, however harvesting of pods should be done as
soon as maturity is reached. This stage is attained when leaves get dried along with 50% of the pods
dry up and turn brown in colour with moisture content not more than 14 per cent [30]. Harvesting of
the crop should be done by leaving 5-10 meters border area. Harvested pods then dried in sun to
reduce the moisture content to desirable content i.e., 8-9 % and threshed either manually or by
thresher [34], [58]. After threshing the seeds must be graded, cleaned and should be treated with
fungicides like Carbendazim before storage. Total yield of the crop varies upon the conditions it is
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
grown viz; under rainfed conditions 7-8 q seed yield/ha is obtained and under irrigated conditions 12-
15q seed yield/ha is obtained [33].
In case self-pollination is completed in the flower it will show signs such as non sticky,
swollen stigma with light green colour and remnant pollens attached. The steps involved in
emasculation are to tear the sepals with forceps from of the keel. Handling with fingers is careful
operation, then little pressure is applied gently that helps in exposing of keel, which is slit-open by
tips of forceps. All the 10 stamens are collected and discarded. Collection of desired pollen at any
time of a day, preferably fresh pollen that is rich moist yellow is gathered from newly opened flower.
After removal of standard and wings, carefully with the help of stylar brush the pollen is kept on
emasculated flower bud to get a hybrid seed. Most successful results are found when pollination in
french bean is obtained before 11 AM. It is very much important to note that the receptive part of
stigma is the elliptical stigmatic tip rather than the hairs on concave side [8]. Under natural
conditions pollination completes 24 hours before flower opens and fertilization is achieved in eight to
ten hours [21].
19.2. Heterosis:
In self-pollinated species to get a hybrid use of heterosis is predominant [46],[47]. But it is
difficult to apply to conduct studies on F1 hybrid and for commercial seed production [48]. For a
hybrid to perform as a best homozygous single plant, it should exceed the mid-parent value or highest
parent value. [47] F1's are superior to the best parent but the results are applicable only to specific
inbred lines studied to get a hybrid. To express the heterosis both general and specific combining
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
ability are important features [49], [48]. Although in pea F1 an additive and non-additive effect
influence on high yielding inbred lines, which would be difficult to produce a pure line due to non-
additive effects. In F2 reports says that the occurrence of inbreeding depression. So, there is possibility
of exploitation in field.
Other techniques by which hybrids are produced are mutation breeding and molecular
markers. Some important traits of cluster beans are developed by obtaining genetic variability through
mutation breeding. Although this breeding method has shortcomings of not showing any major effect
on the breeding and seed setting. Hence breeders have to rely on the advance method of hybrid seed
production i.e., by using the hybrids produced by using fourth generation molecular markers such as
SSR and SNPs [53].
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
Legume seed bags if they not stacked in warehouse are preferably stored for short term in
outdoor storage in two ways; in pyramids mounted on a concrete platform or in flexible silos of 500
tonnes capacity. Other methods of storage are ware house, low-capacity silos and high-capacity silos.
If seeds are stored inside building then ventilation should be the primary concern. A proper
ventilation of godown can minimize the temperature inside hence; prevent the biochemical
degradation of seeds. The optimum level of moisture at which French bean, peas and cluster bean
seeds should be stored is mentioned in table 5. Another advantage if in house storage is that
fumigation of the seeds of beans and peas can be done effectively. Fumigation is indeed necessary to
kill the storage pests of beans and peas. These pests are; Brichids or Pulse beetle (Callosobruchus
spp.), Trogoderma spp., Ephestia spp., Stegobium spp., etc. It requires certain specific requirements
for using chemicals such as Alphos, methyl bromide for getting rid of infestation. As these are
volatile chemicals hence, quick in action, highly effective and easily leak out. The exposure time of
these fumigants depend on temperature, dose, level of infestation etc. For example, if the storage
temperatue is less than 5 ⁰ C then there is no need to fumigate as it will not be effective. But if room
temperature is 20 ⁰ C then an exposure period of four to five days in optimum. The effectiveness of
treatment is more when storage temperatures are high. Fumigation is done with aluminium phopshide
@ 3 tablets (3 gram each) per tonnes with exposure up to seven days or depending upon the room
temperature as mentioned above. After fumigation the airtight conditions (by covering with
tarpauline) provide accurate enviroenmet to kill the eggs and adults of the insects [60].
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Chapter 8: Seed Production Technology of Peas and Beans: French Bean, Pea and Cluster Bean
field inspection and continuous vigilance by the field inspectors and officials where various factors
are critically observed for permissible limit as mentioned in the table below [34]:
Table 5: Seed certification standards for French bean, pea and cluster bean.
Seed certification aims at providing good quality seeds of a notified variety to farmers. Hence
certification of seed is not only restricted to the harvested seed but it aims at control of seed
production at field level as well as managing the land on which seed production will be taken up. It
can be done by meeting the specific requirements of the crop.
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