Potentiality of River Tamarind (Leucaena leucocephala) leaves extract to increase platelet

count

An Investigatory Project Presented

To the Faculty of

B.S. Aquino National High School

In Partial Fulfillment of the

Requirements

in Research I

By:

Jomel V. Soliman
 Introduction
Low platelet count can cause nosebleeds, bleeding of gums, and bleeding of wounds that

last for a prolonged period.

The Researcher think for a widely available product that can increase platelet count such as

River Tamarind. River tamarind has approximately 48.3% of Vitamin B9 or Folate and the

remaining percentage are various types of phytochemical such as Flavonoid, Alkaloid, Terpenoid

(N. Shetta, 2009). Vitamin B9 or folate which greatly helps boost the blood platelet count. Some

other foods rich in B9 are spinach, asparagus, and oranges (L. Olcay, 2013). River tamarind are

commonly known as White Lead tree. It is native to Southern Mexico and Northern Central

America and spread across many tropical and subtropical locations. It has multipurpose uses,

such as generation of firewood, timber, greens, fodder, and green manure, as well as to provide

shade and control soil erosion. It has been used for medicinal purposes because of possessing

multiple pharmacological properties. Studies have shown the presence of various secondary

metabolites such as alkaloid, cardiac glycosides, tannins, flavonoids, saponins, and glycosides

(S. Sallam, 2016).

Carica papaya to increase platelet count is one of the study that can help this research.

The papaya leaves extract was specialized because Papaya leaves is reach in Vitamin B9. Leaves

contain also an alkaloid called carpaine. The testing of the papaya leaves extract takes 10 hours

to know it's effectivity (R.Waduge, 2015).

The Researcher was inspired to make an alternative substance that can increase platelet

count. This Research will be a big help in terms of medicinal purposes, this research can prevent

nosebleed, bleeding of gums and bleeding of wounds that prolonged in a period of time.
Statement of the Problem

1. Is there any potentiality that River tamarind leaf extract increase platelet count?

2. Is there any significant difference in using River tamarind leaf extract compare to

commercial product in terms of:

• Effectiveness

• Amount

• Duration

Ho: There will be no significant difference between the River tamarind leaves extract to

increase platelet count and the commercial ones

Ha: There will be a possibility to use River tamarind leaves extract to increase platelet

count.

Significance of the Study

This Research is all about having a substance that can increase platelet count that is

cheaper that commercialized products. People that are suffering in bleeding of gums and

bleeding of wounds that prolonged in a period of time can be the beneficiary of this research.
Scope and Delimitation

This Study Focuses in making a substance that can increase platelet count to avoid

bleeding of wounds that prolonged in a period of time

The river tamarind leaves will be collected San Jose Concepcion, Tarlac. The Testing was

done in Vet Soucier Veterinary Clinic

MATERIALS AND FUNDINGS

MATERIALS PRICE

Mortar ₱0.00

Pestle ₱.00

Syringe ₱20.00 Methodology

Cheese Cloth ₱0.00
Research Design
Sterilized Empty Container ₱0.00

6 Laboratory Mice ₱ 330.00 The

experimental method
Commercial Product ₱ 97.3
will be used in this
Total ₱ 447.3 study. This method
can find if there’s any significant effect when River tamarind leaves extract will be injected in a

laboratory mouse.

Materials and Procedures

First, the research will be collecting 200 grams of River tamarind leaves. Next, the

researcher will do the pounding and extracting procedures to get the River tamarind leaves

extract. After, the extracting and pounding process the researcher will put the River tamarind

leaves extract in a cheese cloth to avoid the residue

Testing of the Product

There will be a two laboratory mouse, the researcher will be injecting the river tamarind

leaf extract to the first mouse. The other mouse will not be injected. After 8 hours, the researcher

will compare the two laboratory mouse. Hemocytometer will be the instrument that will count

platelets.

Date Gathering Procedures

The Veterinarian will be evaluated if the platelet count of mouse increases

Statistical Treatment

T- test method will be used in this research to analyze and interpret the results
 Results and Discussion

Table 1: Shows that in different trials have equal length of time and have only

varying amount of extract

Interpretation:
Based on the data in the table, it shows the different trials that has the same length of time and

have only varying amount of taro leaves extract. In trial A it used 2ml of River tamarind leaves

extract. Trial B used 3ml of River tamarind leaves extract. And trial C used 4ml of River

tamarind leaves extract. All of the trials had 8 hours between before testing it again for the final
Trial Amount of Initial Platelet Length of Result of the
River count Time in Platelet count
tamarind Processing the
leaves Extract Extract in
Mice
A 2ml 160,000 mcl 8 hrs 172,000 mcl
B 3ml 155,000 mcl 8 hrs 173,000mcl

C 4ml 163,000 mcl 8 hrs 187,000mcl

result.

Table 1.2

Trial Amount of Initial Platelet Length of time in Result of the

Commercial count processing the Platelet count
Product extract in mic
A 2ml 150,000 mcl 8hrs 189,000 mcl
 B 3ml 142,000 mcl 8hrs 178,000 mcl
C 4ml 164,000 mcl 8hrs 218,000 mcl

Interpretation:

Based on the data in the table, it shows the different trials that has the same length of time and

have only varying amount of commercial product. In trial A it used 2ml of commercial product.

Trial B used 3ml of commercial product. And trial C used 4ml of commercial product. All of the

trials had 2 hours between before testing it again for the final result.

Table 2

Comparison of effectiveness between the River tamarind leaves extract and commercial
product in increasing platelet count
 Platelet Platelet Computed Sum Computed Sum
count of count of of the River of the
the treated the treated Tamarind leaves Commercial
mice using mice using extract ones
taro leaves commercial
extract ones (x1 – x̅ )²
(x1 – x̅ ) (x2 – x̅ )
( x1) ( x2) (x2 – x̅ )²

172,000 189,000 mcl -5,333.333mcl -6,000mcl 28,444,440.89mcl 360,000,000mcl

mcl
178,000 mcl -4,333.333mcl -17,000mcl 18,777,774.89mcl 289,000,000mcl
173,000mcl

187,000mcl 218,000 mcl 9,666.667mcl 23,000mcl 93,444,508.89mcl 529,000,000mcl

Σx= Σx= Σ(x1 – x̅ )2 = Σ(x1 – x̅ )2 =

532,000mcl 585,000mcl 140,666,724.67mcl 1,178,000,000mcl

Interpretation:

Based on the data in the table, it shows the comparison of the River tamarind leaves

extract and commercial product in increasing platelet count of the six mice. It also shows in the

data that the commercial product is more effective and it increase platelet count more of the mice

than the River tamarind leaves extract

 Table 3
T- test on increasing platelet count between the River Tamarind Leaves Extract and

Commercial Product

Group N Mean Σ(x – x̅ )2 T- test

Experimental 3 532,000mcl 140,666,724.67mcl 4539.9

Commercial 3 585,000mcl 1,178,000,000mcl

Interpretation:

The computed value is 4539.9 which is more than the tabular value of t, thus, it is

significant. This means that the null hypothesis is rejected and there is a significant difference

between taro leaves extract and the commercial product.

 Summary, Conclusion and Recommendations

Summary

The materials used to make the product was 189g of River tamarind leaves and 4ml of
water. This research undergoes pounding and extracting process to get the extract of River
Tamarind leaves extract.
The River tamarind leaves extract was injected on 3 mice, and 3 mice also for the

commercial product, over all this research used 6 mice. Three trials was done in this research to

determine the following: Effectiveness, Duration, and Amount. The commercial product which

was the controlled variable for this study was used to compare the results of the experimental

variable as an alternative substance to increase platelet count.

The River tamarind leaves extract was tested and proven to have a potentiality to increase

platelet count.

Conclusion

1. The River tamarind leaves extract to increase platelet count varies in many qualities

2. The River tamarind leaves extract was proven effective on increasing platelet count based

on the results

Recommendations

After the study, the researcher recommends the following:

1. Need to have a further study about River tamarind leaves.

2. Look for another method in extracting the River tamarind leaves.
3. Use more relevant materials in doing the extracting process
 Bibliography

https://www.hindawi.com/journals/tswj/2014/781857/

https://www.bloodjournal.org

https://medlineplus.gov/druginfo/meds/a609008.html

https://www.rediff.com/getahead/report/health-9-foods-to-increase-blood-platelets/

20160316.htm

https://www.rediff.com/getahead/report/health-9-foods-to-increase-blood-platelets/

20160316.htm

https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/phytochemical

http://www.stuartxchange.org/Ipil-ipil.html
 Appendix

T-test result in the River tamarind leaves extract and Commercial one

2
Σ(x1 – x̅ )2 2
Σ(x2 – x̅ )2
SD1 = SD2 =
N-1 N-1
= 140,666,724.67 = 1,178,000,000
3-1 3-1

SD12 = 70,333,362.3 SD22 = 589,000,000

t=
x̅ 1 – x̅ 2

√
SD12 + SD22
N N
= 127.67-93.33

√
70,333,362.3 589,000,000
+
3 3
87,324,987.26
=
19,234.6

t = 4539.9