MT.

CARMEL AGRI-TOURISM TRAINING CENTER

BANGUED, ABRA

TRAINING ON
MUSHROOM
PRODUCTION
ALFRED T. TOLENTINO
National Trainer
National Competency
Assessor
Agricultural Crops
Production
 WHAT ARE MUSHROOMs?
Mushrooms are edible fungi, that grow and
obtain food from decaying organic
matter.
FACTORS INFLUENCING MUSHROOM
GROWTH
• Temperature
• Light
• Humidity
• Food
• Management
 Different species of mushroom

Pleurotus sajor caju Volvariella volvaceae

(Oyster mushroom) Umbrella mushroom
 Different species of mushroom

Auricularia sp. Ganoderma sp.

(Tengang daga)
 Different species of mushroom

Pleurotus sp Pleurotus sp.

(Yellow Oyster mushroom) Pink oyster mushroom
 Different species of mushroom

Pleurotus eryngii Pholiota nameko

(King Oyster mushroom)
Different species of mushroom
Different species of mushroom
 Different species of mushroom

Agaricus bisporus Hericium erinaceus

White buttons Monkey Head
 Mushrooms & Vitamins
Vitamin D: for healthy bones and teeth.
Vitamin B1: Thiamin needed for the normal functioning
of the brain and nervous system.
Vitamin B2: Riboflavin helps maintain healthy red
blood cells and promotes good vision and
healthy skin.
Vitamin B3: Niacin keeps the body’s digestive and
nervous systems in good shape.
 Mushrooms & Vitamins
Vitamin B5 – Pantothenic Acid: responsible in the
production of hormones
Vitamin B9 - Folate: essential for the formation of red
and white blood cells in bone marrow.
Vitamin H – Biotin: Is essential in the metabolism of
proteins and carbohydrates and is just another B-
vitamin found in mushrooms.
 MUSHROOMS & MINERALS
Sodium: Mushrooms contain virtually no salt.
Potassium: aids in the maintenance of normal fluid
and mineral balance, which helps to control
blood pressure. Mushrooms contain more
potassium than most other fruit and vegetables.
mushroom contains more potassium than a
banana.
Calcium: 100g of mushrooms contains 2mg of
calcium.Calcium provides the structure for teeth
and bones and is needed for muscle
contraction.
 MUSHROOMS & MINERALS
Iron: Mushrooms are good source of iron,
which is essential to most life forms.
Zinc: stimulates the activity of
approximately 100 enzymes that
supports a healthy immune system.
Magnesium: helps maintain normal
muscle and nerve function, keeps heart
rhythm steady, supports a healthy
immune system and keeps bones
strong
 MUSHROOMS & MINERALS
Selenium: This mineral works as an
antioxidant, protecting body cells from
damage that might lead to heart disease
and some cancers.
Ergothioneine: naturally occurring,
antioxidant which is found in
mushrooms.
 PART II
STEPS AND PROCEDURES IN
MUSHROOM PROPAGATION
(TISSUE CULTURE METHOD)

16
STAGES IN MUSHROOM CULTIVATION
Culture media preparation
PURE CULTURE Sterilization
FRESH MUSHROOM Tissue culture
Incubation (14 days)

RAPID MULTIPLICATION Inoculation

Incubation (14 days)
“subcultures”

Substrate preparation
Sterilization
SPAWN PREPARATION Inoculation
Incubation (2-3 weeks)

Indoor
Outdoor
PLANTING FOR FRUIT Fruiting Bags
PRODUCTION Logs
 I. PURE CULTURE PRODUCTION

Common media used: Low cost supplies /

(Potato Dextrose Agar) substitute
POTATO
SUGAR POTATO – Kamote, Rice
GULAMAN BARS wash, Mongo extract, corn
DISTILLED WATER extract, etc..
Equipments/ Supplies AUTOCLAVE –
Needed:
Stove, Casserole, match, Casserole, ordinary
autoclave, flat bottles pressure cooker
 A. Culture Media Preparation

Materials needed:
¼ kg rice grains
2.5 grams gulaman bars
3 tablespoon white sugar
1 liter distilled water
20 flat bottles
Cotton
Aluminum foil/ paper
Autoclave
Procedure:
 B. Tissue Culture Method

Materials needed:

Fresh Mushroom
Flat bottle containing sterilized rice wash agar
Alcohol
Alcohol lamp
Denatured alcohol
Inoculating needle
Scissor

02/26/23
Inoculation Chamber

Front View Side View

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Procedure:
 II. MUSHROOM SPAWN PRODUCTION

Materials needed:

2 kg cracked corn
4 liters water
3 drops of chlorox
Polypropylene bags (4” x 10”)
Rubber band
Alcohol lamp
Denatured alcohol
Alcohol
Pure Culture
Procedure:
 D. PREPARATION AND INOCULATION OF
FRUITING BAGS
Materials needed:

48.75 kg rice straw

40 kg saw dust
10 kg rice bran
1 kg brown sugar
0.25 lime
Polypropylene bag (6”x 12”) 0.018 thickness
PVP pipe (3/4” diameter)
Rubber Band
Cotton
Steamer drum
Alcohol lamp
Denatured Alcohol
Alcohol
Grain spawn
Procedure:
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E. HANGING OF FRUTING BAGS,
HARVESTING AND WATERING
MUSHROOM
HOUSE
Shelving Style
Shelving Style
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Shelving Style
Hanging Style
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MUSHROOM
PRODUCTS
Mushroom Meals

51
Canned Mushroom

52
Pickled Mushroom

53
Adobo Mushroom

54
Mushroom Jam

55
Mushroom Siomai

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Mushroom Yema

02/26/23
Mushroom Candy

58
Mushroom Cookies

59
Mushroom Pulvoron

60
Mushroom Wine

61
Mushroom Barquillos

62
Mushroom Cracker

63
64
Mushroom Noodles Mushroom Stir-Fried Noodles

65
Mushroom Fruit Juice

66
Mushroom Embutido Mushroom Burger Patty

67
Dried Mushroom
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 Cost of
Production

71
 Cost of Production - Oyster Mushroom (1,000 Fruiting Bags)

Description Quantity Unit Estimated Estimiated Cost

Unit Cost
A. Materials
  1. Rice straw (Free)        
  2. Saw dust 223 kg 10.00 2230.00
  3. Rice bran 56 kg 10.00 560.00
  4. Polypropylene bags (6"x12"X0.02") 10 pack 60.00 600.00
  5. Steel drum 1 pc 750.00 750.00
  6. PVC pipe (3/4 inch diameter) 5 pcs 160.00 800.00
  7. Rubber band 15 box 8.00 120.00
  8. Cotton 8 roll 60.00 480.00
  9. Denatured alcohol 2 bottle 25.00 100.00
10. Alcohol lamp (or improvised
  "kingki") 1 pcs 75.00 75.00
  11. Nylon rope # 8 2 roll 350.00 700.00
  12. Sugar 6 kg 46.00 460.00
  13. Lime 5 kg 35.00 175.00

  14. Fire wood 100 bundle 15.00 1,500.00

  Sub- total 8,886.00

B. Labor

  1. Hauling and chopping rice straw.  2  MD  250.00  500.00

2. Bagging, sterilization and
2500.00
  inoculation of fruiting bags 10  MD  250.00
Sub-
  total 3,000
  TOTAL 11,886.00

Cost of
Production
Cost of production/ Fruiting bag =
No. of fruiting
bags
11,886.00
=
1000
= 11.89
Estimated Income
1. Fruiting bags

Price / fruiting bag - PhP 20.00

Fruiting bags produced - 1000
Sales = Quantity x Price
= 1,000 fruiting bags x 20.00
= 20,000
Net income = Sales - Cost of Production
= 20,000 – 11,886.00
= 8,114.00
2. Fruits harvested
Price/kilo of mushroom -
PhP160.00
Estimated harvest/fruiting bag within 3 months from 1st flushing
of fruit – 250 g
850 fruiting bags x 250g = 212.5 kilo
Sales = Quantity x Price
= 212.5 kg x 160.00
= 34,000
Net income = Sales - Cost of Production
= 34,000 – 11,886.00
= 22,114.00
“Uong Mangipaay Taraon ken
Pamasterakan ni Mannalon”

02/26/23
THANK
YOU!