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Method of Producing Chitin and Chitosan from Green Bay Mussel (Perna
viridis) Shells

Patent · May 2015

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Vivian Lucaban Moya

Samar State University
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[12] UTILITY MODEL REGISTRATION [11] Registration No.: 2/2014/000662

[45] Issue date: 27/0512015

[54] Title: METHOD FOR PRODUCING CHITIN AND CHITOSAN

FROM GREEN BAY MUSSEL (PERNA VIRIDIS)
SHEllS

[72] Maker(s): VIVIAN L. MOYA [Catbalogan City, Samar (Western

Samar), Philippines]

[71] Applicant(s): SAMAR STATE UNIVERSITY [Catbalogan City, Samar

(Western Samar), Philippines]

[22] Filing Date: 26/11/2014

[43] Publication date: 27/05/2015

[21 ] Application No.: 2/2014/000662

[30] Foreign Application Priority Data: NONE

[51 ] International Class: C 08B 37/08, C 08l 5/08

[57] ABSTRACT

A method for producing chitin and chitosan from green mussel (Perna viridis) shell
comprising the steps of: cleaning green bay mussel shells thoroughly with distilled water;
drying said green bay mussel shells in a controlled dryer at 35-40°C 'for 1 hour; pulverizing
said dried green bay mussel shells until powder consistency using a pulverizer with 2mm
mesh screen; soaking said powdered green bay mussel shells with 8% hydrochloric acid
(1:12 w/v) for 1 hour at room temperature; straining said powdered green mussel shells from
the solution using a filter funnel; washing said powdered green mussel shells with distilled
water until a neutral pH is obtained; straining said washed powdered green bay mussel
shells using a filter funnel to remove excess water; heating said powdered green bay mussel
with 8% sodium hydroxide solution at 80-100°C in boiling water bath until dried to produce
chitin; washing said chitin with distilled water until a neutral pH is obtained; drying said chitin
under the heat of the sun for two (2) hours; bleaching said chitin with 3% sodium
hypochlorite solution for 5 minutes; washing said chitin with distilled water; straining said
chitin using a filter funnel to remove excess water; drying said chitin under the heat of the
sun for two (2) hours; heating said chitin with 50% sodium hydroxide (1:12 w/v) in boiling
water bath until dried to produce chitosan; washing said chitosan with distilled water until
neutral pH is obtained; drying said chitosan under the heat of the sun for one (1) hour.

Description: 5 page(s). Drawings: 1 sheet(s)

Examiner: C. D. GARCIA

Attorney/Agent:
 IPfi)
PHL~'
Library Copy
[21] Application Number: 2/2014/000662 MAY 2 7 2015
[22] Filing Date: 26 November 2014
METHOD FOR PRODUCING CHITIN AND CHITOSAN FROM GREEN BAY MUSSEL
[54] Title:
(PERNA VIRIDIS) SHEllS

[71] Applicant(s): SAMAR STATE UNIVERSITY [CATBAlOGAN CITY, Samar (Western Samar), PH]

[72] Ma¥r(s): VIVIAN L. MOYA [CATBAlOGAN CITY, Samar (Western Samar), Philippines]

[73] Assignee(s):

[74] Attorney I Agent:

[30] Priority Data:

[51] International Class 8: C 08B 37/08, C D8l 5/08

A method for producing chitin and chitosan from. green mussel (Perna viridis) shell comprising the steps
of: cleaning green bay mussel shells thoroughly with distilled water; drying said green bay mussel shells
in a controlled dryer at 35-40°C 'for 1 hour; pulverizing said dried green bay mussel shells until powder
consistency using a pulverizer with 2mm mesh screen; soaking said powdered green bay mussel shells
with 8% hydrochloric aci (1:12 wlv) for 1 hour at room temperature; straining said powdered green
mussel shells from a filter funnel; washing said powdered green mussel shells with distilled water until a
neutral pH is obtained; straining said washed powdered green bay mussel shells using a filter funnel to
[57] Abstract: remove excess water; heating said powdered green bay mussel with 8% sodium hydroxide solution at
80-100°C in boiling water bath until dried to produce chitin; washing said chitin with distilled water until a
neutral pH is obtained; drying said chitin under the heat of the sun for two (2) hours; bleaching said chitin
with 3% sodium hypochlorite solution for 5 minutes; washing said chitin with distilled water; straining said
chitin using a filter funnel to remove excess water; drying said chitin under the heat of the sun for two (2)
hours; heating said chitin with 50% sodium hydroxide (1:12w/v) in boiling water bath until dried to
produce chitosan; washing said chitosan with distilled water until neutral pH is obtained; drying said
chitosan under the heat of the sun for one (I) hour.

Representative Drawing(s):

F.... I

No. of Claims:

Relevant docs
International Standard Industrial
Classification (ISIC) C-2029- Manufacture of other chemical products n.e.c.

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