LAB REPORT WRITING

SIC1002

INORGANIC CHEMISTRY I

SEMESTER 2

SESSION 2023/2024

TITLE:
CALIBRATION OF A 25-ML PIPETTE

NAME : MUHAMMAD AIMAN BIN DAUD

MATRIC NO. : U2001076
OCCURANCE :1
LECTURER’s NAME : DR. MUHAMMAD AMEERULLAH BIN SAHUDIN
DATE : 11 / 03 / 2022
 EXPERIMENT 1

Title: Calibration of a 25-ML Pipette

Objective:
1. Able to handling a pipette, including - selecting the appropriate pipette for a given task, and
using the selected pipette in the proper manner.
2. Able to understand the accuracy of pipettes in the laboratory by relating pipetted volumes to
mass measurements.
Results:
TRIAL 1 2 3
Room Temperature (°C) 25 25 25
Mass Weighing Bottle + 45.0525 45.0525 45.0525
cover (g)
Mass Weighing Bottle + 69.9370 69.8620 69.8635
cover + water (g)
Mass of water (g) 24.8845 24.8095 24.8110
Average mass of water 24.8353
(g)

1.06(w )
W= w +
1000
Density of distilled water = 0.99597 g/mL
W= 24.8353 + 1.06(24.8353)/1000
=24.8616
ρ = m/V
0.99597=24.8616/V
Estimated volume = 25.0000
Obtained volume =24.9622
Difference = 0.0378
24.9622
Accuracy Calibrated volume = × 100%
25
=99.84%
Discussion:
From the calculation, the volume obtained in the experiment is different from the expected
volume of the pipette which is 25.0 mL, but the accuracy of obtained volume is near to
100% which is 99.84%. basically,this differences occur due to certain mistakes while the
experiment, such as parallax error, when we take the meniscus water level to approach the
graduated point.

Precaution:
1. The tip of the pipette was not immersed too deep in the water and was held above the
water level to prevent any mishap during the suction of the pipette water through the
pipette filler
2. The tip of the pipette was touched on the dry side of the container before the last drop
at the tip had been drained
3. During transmission, the tip of the pipette was held above the water level in the
receiver

Conclusion:
The calibration of the 25 mL pipette revealed it to be both accurate and precise, with an
average dispensed volume of 24.9622 mL and a standard deviation of 0.0378 mL. While the
pipette shows high reliability, minor deviations were observed, likely due to environmental
conditions and human error. Regular calibration and adherence to proper pipetting
techniques are recommended to maintain accuracy and precision. This calibration exercise
underscores the importance of routine equipment verification in achieving reliable
experimental outcomes.

Post lab questions:

1. Does the pipette empty from full volume to zero or from zero to full volume?

Take care to look at the volume scale before use some graduated pipettes empty from full
volume to zero.

Take care to look at the volume

scale before use: some graduated
pipettes empty from full
volume to zero, others from zero
to full volume; some scales refer
to the shoulder of the tip,
others to the tip by gravity. Never
blowout volumetric (bulb)
pipettes, just touch the tip
against the inside wall of the
vessel
2. Is the pipette designed to be emptied by gravity with the tip in contact with the vessel or to
be expelled by blowing out with pipette filler? Explain.

The pipette-like bulb or volumetric pipette used in this experiment is designed to be emptied
with the tip in contact with the vessel because a small portion that remains at the tip should
not be blown out because the pipette has been calibrated to deliver the appropriate amount of
solution with some remaining in the tip due to surface tension.

References:
Clark, P. & Shull A. H. (2003) Gravimetric & spectrophotometrit errors impact on pipettecalibration
certainty. Cal. Lab, 10(1), 36-43
Mangakiya, K. K. & Panchal, M. (2016) Impact of calibestan of pipette ont iguality control results
lat. 1. Clin. Barchem Bes, 3(1), 28-30 pg, 273, 148-158

Penger. D. 1. Bouman, RL, A Verk, GO (1965) Costast volume, self-filling samt pipette Construction
and calibration Science 1473618) 406-608