See discussions, stats, and author profiles for this publication at: https://www.researchgate.

net/publication/233796286

Essential oil composition of Homalomena aromatica roots

Article · January 2009

CITATIONS READS

8 3,550

5 authors, including:

Virendra S. Rana Huidrom Birkumar Singh

Indian Agricultural Research Institute, New Delhi, India North East Institute of Science & Technology
143 PUBLICATIONS 1,374 CITATIONS 35 PUBLICATIONS 509 CITATIONS

SEE PROFILE SEE PROFILE

Mercedes Verdeguer María Amparo Blázquez

Universitat Politècnica de València University of Valencia
52 PUBLICATIONS 840 CITATIONS 133 PUBLICATIONS 3,194 CITATIONS

SEE PROFILE SEE PROFILE

All content following this page was uploaded by Virendra S. Rana on 30 May 2014.

The user has requested enhancement of the downloaded file.

I
Essential oil composition of Homalomena aromatica roots

V.S. Rana', M. Pukhrambamb, H. B. Singh!>,M. Verdeguer" and M.A. BIazquez"

ABSTRACT
The chemical compositions of the essential oil of Homa!omena aromatica root were dctern1ined by GC/MS. The yield
oCthe oil in root was found to be 0.64'1/0on dry \vcight basis. Thirty compounds accounting for 88.7% of the oil were
Identi fied. The main compounds were linalool (58.3%), terpinen-4-ol (16.7), a-terpineol (1.8). a-eadinol (1.7),
spathulenol ( 1.5) am.!cryptone (1.4).

INTRODUCTION essential oil from Bangladesh". To our best knowledge

there is no report on the analysis of its root oil from NE
The genus Homa!omenu (Family: Araceae) consists of India and thus it was thought to identify the chemical
about eighty species found in the Asia and South components of H. aromatica root oil using GC/MS.
America of which two species are reported in India'.
F!ol/la{o/1lel1o
aromaticu (Spreng.) Schott. commonly
known as Sugandhmantri. is a rhizomatous perennial !\'tATERIALS AND METHODS
herb found in Assam. Chittagong hill of Bangladesh and
Jampui in Tripura". It has also been found growing in
General /::"xperimelltal Procedure
lower altitudes of Arunachal Pradesh. Nagaland,
\1il.Oram and Manipur. Its rhizomes are aromatic
Fresh rhizomes were collected from Jiribum (Imphal
stimulant and found to possess mosquito repellant
East), Manipur in January 2008. The plant material was
properties':. The juice of whole plant is used for the
authenticated by North-East Institute of Science and
treatment of skin diseases. Essential oil is used in
Technology, Sub-station, Imphal. The cleaned air dried
perfumery'. Earlier reports indicated that its root
roots were cut in to small pieces and roots pieces (300g)
contains linalool and terpinen-4-01as ~najorcompounds were subjected to hydrodistillation for 4 h. in a
besides sesquiterpenoids' . Recently, Chowdhury el a!., Clevenger-type apparatus. The distillate was extracted
2008 has identifiedlinalool (69.5%), 4-terpineol (7.6%), with diethyl ether, the ethereal layer was dried over
2-furanethanol-5-etheny Itetrahydro-a.(1-5-tri methy I anhydrous sodium sulphate, distilled gently on water
(5.7%). ft-methyl-ft-( 4-methyl-3-pentenyl}- bath. The yield of the oil obtained from the roots was
oriranemethanol (5.3%)and 3.7-octadiene-1,6-diol-2.6- foundto be 0.66 % on dry weight basis and were stored at
dimcthyl (2.80/0), a-cadinol (2.7%) and I, 2- 4"e,
dimethylbenzene (I.O'~;'»)as main compounds in its

Corresponding address: Directorate of Medicinal and

Aromatic Plants Research. Boriavi-387 3 10, Anand,
Ciujarat. India. Ph: 02692 271605, Fax: 02692271601
(01. E-mail: ranavs2000(1I.yahoo.com
'Natural Product Chemistry Lab. Medicinal Pla'nts and
Ilurticultural Resources Division, Institute of
Blon:sourccs of Sustainahle I)nL'lopment, Takyclpat
Institutional Area.lmphal-79500 I. Manipur.lndia.
North-East Institute of Science and Tcehnology, Sub-
station. Imphal. Manipur.lndia.
Analysis of the volatile oil was performed on a GCiMS
(Varian Saturn 2000) ~quipped with a Varian C.SVA-
5MS capillary column 130 m x 0.25 mm i.d" film
thickness 0.25 m). Chromatographic conditions wen: as
f()lIows:helium as carrier gas at a flow-rate of2 ml/min
(split mode); injection volume was 0.51; 1I1jector
temperature was 250 "c respectively. The column
temperature was held at60r,Cfor 5

Department de Fannacologia. Faeultat dc Farmacia,

Univcrsitat de Valencia. Valencia. Spain.
 f
,
,/
v' min., and programmed at 3 <lC/minto 180°(' and then 20 REFERENCES
"Gmin to 280 lie and held for I0 minutes. The column
was coupled directly to the quadrupole mass
I. K.R. Kirtikar and B.D. Basu. Indian Medicinal
spectrometer at EI mode at 70eV with the mass range of
::!X-400a.1ll.u rang~ at I scan!s. Kovats retention indices Plants. Vol. IV. pp. 2619-2620 (2004). Bishen
were ~alculated using co-chromatographcd standard Singh Mahendra Pal Singh. Dehra Dun.
hydrocarbons. The individual compounds (Table I) were Uttrakhand, India.
identified by maSS spectra and their identity was
confinl1ed by comparing their retention indices relatives 2. H. Lalramnghinglova, £thno-Medicillal Plallls
to C.-C" n-alkanes'. Identification of individual of Mizoram, PI' 175-I76 (2003),BishenSingh
compound was further carried out by matching mass Mahendra Pal Singh, Dehra Dun, Uttrakhand,
fragmentation pattern with those from the available
authentic samples as well as with from NIST Library and
India.
literature data'. 3. G. Singh, I.P.S. Kapoor, G.P. Singh, G.P. Rao.
YR. Prasad, P.A. Leclercq and N. Klinkby,
Studies on essential oils, part 28: Chemical
RESULTS AND DISCUSSION composition, antifungal and insecticidal
0'\
o activities of rhizome volatile oil of llo/t1alome/ltl
o
N The yield of the oil in the roots of Homaiomena
aromatica Schott. Flav. Frag. J. 15(4), 278-280
;....
(l)
arumatica was found to be 0.66% on dry weight basis. In
..J::. GCiMS analysis. thil1y compounds accounting for (2000).
E
(l)
88.7% of the oil were identified (Table I). The main 4. M. Todorova, P.TT Tho, I. Ggnyanov and 8.M.
u compounds were linalool (58.3%), terpinen-4-01(16.7),
(l)
o a-terpineol (1.8), a-cadinol (1.7), spathulenol (1.5) and Lawrence. The composition of Homaiomena
cryptone (1.4). epi-a-cadinol (1.0) and oplopanone (1.0) aromalica Schott. oil of Vietnamese origin. Flav.
along with two trace compounds. Comparing the
chemical composition of thc its oil with those studied Frag.J.3(4), 179-181(1988).
earlier showed that linalool and terpinen-4-01have been 5. T.v. Sung, B. Steffan, W. Stcglich, G. Klebe and
reported as major compounds in all the analyzed oils.
G. Adam. Scsquilcrpenoids froll1 the roots or
however there is great qualitative and quantitative
diffcrencc among the chemical component identified"". Homalomella aromatica. Phytochemistry
It is worthy to notc that I, 2-dimethylbenzene( I.0%),2- 31( I0), 3515-20 (1992).
furanethanoI -5-cthen yl tetrahydro-a.a. 5-tri mcthy I
6. 1.U. Chowdhury, M. Yusuf and M.M. Hossain,
(5.69(%), a- methy I-a-( 4- methy 1-3-pen teny I}-
oriranemcthanol (5.29%) and 3,7-octadiene-2,6-diol- Aromatic Plants of Bangladesh: Constituents of
2,6-dimethyl (2.87%) identified recently as other main rhizomes oil of Homaiomena aromalica. Indian
compounds by Chowdhury et ai., 2008 were found
absent in this oil". It is concluded that linalool followed Perfum. 52( 1),33-34 (2008).
by terpinen-4-ol and a-terpineol are major compounds 7. R.P. Adams. Identification oj" Esselltial Uil
in the analyzed oils from India and abroad and this study
!o.- Components by Gas Chromatographv lA/ass
furtherconfirmedthat this speciesis samechemotype,
(])
however the quantitative variation of the individual
E
::J
compounds observed among the analyzed oils may be
due to different harvesting time and geographical
Spectrometry. 2007. Allured Pub. Corp., Carol
Stream, lllinois, USA.
~
!o.- reasons. Thus. H. aromalica plant can be utilized as a
(]) natural source oflinalool and oiJ.'
Cl. ...

C
o
D
c

""""" ~
~f
Table 1: Percentage composition of Homalomena aromatica root oil

cis-linalooloxide 1076 0.8

trans-linalooloxide 1086 0.8 .
linalool
cis-p-menth-2-en-l-01
I 1100 I 58.3 I I
I
1126 I
0.1 I
AEC
trans-p-menth-2-en-l-01 I 1142 I 0.3 I IAsian Aroma Ingredients
p-menth-l,5-dien-8-o1 1170 0.3 CONGRESS& EXPO
tcrpinenA-ol 1174 16.7 . Venue: LEElAKEMPINSKIHOTEl,
. GURGAON,N.C.R.,DELHI.
cryptone I 1185 1.4 , ,

p-cymen-8-o1 1179 0.1 MAY 7 to 9,2010

a-terpineol 1192 1.8 I Website:www.aeoc.2010.com
-
neroI I 1229 I 0.1, I
,
Supporting
Organisations- I.
cuminaldehyde I 1241 0.2
Fragrance
&Flavour
Association
ofIndia,Mumbai.
I I

. 1';.
".. I.i.\..
carvone I 1243 t li"

I.
BasicChemicals,
Phanmaceuticals
&
Cosmetics
ExportPromotion
Council.
Mumbai.
car-3-en-2-one I 1248 I 0.2 I
(CHEMEXCIL)
piperitone 1253 0.2 SpiceBoard of India, Cochin.
p-caryophyllene
, 1419 0.1 .' .,::;,-

...
Central
Institute
ofMed.&Aromatic
Plants.
a-humulene I 1459 0.1 0 (aCSIRInstute), Lucknow.

allo-aromadendrene I 1460 0.1 IndianInstitute

ofIntegrative
Medicines.
(aCSIRInstitute),Jammu.
a-selinene I 1498 I 0.3 I I
I
.
FragraceAndFlavourDevlopment
Centre.
\i
a-muurolene I 1500 Govt of India. Kannauj. I I i.

y-cadinene I 1513 I 0.1 TheSpiceCouncil

ofSrilanka,
Colombo,
Snlanka.

bca'diriene' [ 1'52& Indonesian

Essential
OilCouncil,
Jakarta,
Indonesia.

a-calacorene I 1545 041 I Indonesian

Essential
OilTrade
Association,
Jakarta.

spathulenol I I 1581 I 1.5 I China Association Flavor&

ofFragrance,

lcdol I 1600 I 'CID Cosmetic

Industries,
Beijing.

humuleneepoxideII I 1611 I 0.6 Organlsed by-

epi-a-cadinol I 1640 I 1.0, I I

'!
Essential
DOil
ofIndia, Association
elhi .
a-cadinol I 1654 I 1.7 I I 301,4832124, Ansari Road, Dariyaganj, New Delhi.110002.
E-mail:eoal@bol.net.ln,aeoc2010@hotmail.com
Ph91-11.23250322 Fax:91.11.23250443

oplopanone I 1740 I 1.0

-
hexadecanoic acid 0.1
RI, retention index; t, trace (Jess t\1an 0.05%)

View publication stats