Figure A : Graphs demonstrating the interference due to hemolysis at different values of

analytes tested.
Considering the TCL as maximum percentage acceptable limit, graphs represent the
percentage deviation from baseline value according to the hemolysis index.
Horizontal dotted line indicates TCL value for each analyte in %, established from our data
ALP= alkaline phosphatase, ALT= Alanine Aminotransferase, AST= aspartate
aminotransferase, CRP= C-reactive protein, GGT= gamma-glutamyltransferase, Hp=
haptoglobin, LD= lactate dehydrogenase, PCT= procalcitonin,
HI= hemolysis index,
TCL= Total Change Limit

Albumin ALP
29 g/L 36 g/L 69 IU/L 136 IU/L 213 IU/L
% deviation from baseline concentration

% deviation from baseline concentration

8 10,00
6 0,00
4 -10,00
2
-20,00
0
-30,00
-2
-4 -40,00
-6 -50,00
-8 -60,00
0 1 2 3 4 5 6 7 8 9 10 11 0 1 2 3 4 5 6 7 8 9 10 11
H-Index, g/L H-Index, g/L

AST
ALT
25 IU/L 46 IU/L 178 IU/L
24 IU/L 42 IU/L 191 IU/L
400
% deviation from baseline concentration

100 350
% deviation from baseline concentration

80 300
250
60
200
40 150
100
20
50
0 0
-20 -50
0 1 2 3 4 5 6 7 8 9 10 11 0 1 2 3 4 5 6 7 8 9 10 11
H-Index, g/L H-Index,g/L
 % deviation from baseline concentration Bicarbonates Creati nine
19 mmol/L

% deviation from baseline concentration

70 µmol/L 100 µmol/L
12
10
8
7
6
2 4
2
-3 0
-2
-8 -4
-6
-13 -8
0 1 2 3 4 5 6 7 8 9 10 11 0 1 2 3 4 5 6 7 8 9 10 11
H-Index, g/L H-Index, g/L

CRP Ferriti n
% deviation from baseline concentration

104 mg/L 132 ng/mL 1619 ng/mL

30 15

% deviation from baseline concentration

25 13
11
20
9
15
7
10
5
5 3
0 1
-5 -1
0 1 2 3 4 5 6 7 8 9 10 11 0 1 2 3 4 5 6 7 8 9 10 11
H-Index,g/L H-Index,g/L

GGT Haptoglobin
% deviation from baseline concentration
35 IU/L 122 IU/L 241 IU/L 0.6 µmol/L 1.4 µmol/L 2.4 µmol/L
% deviation from baseline concentration

2 4
1 2
0 0
-1 -2
-4
-2
-6
-3
-8
-4 -10
-5 -12
-6 -14
-7 -16
0 1 2 3 4 5 6 7 8 9 10 11 0 1 2 3 4 5 6 7 8 9 10 11
H-Index, g/L H-Index, g/L

Iron LD
% deviation from baseline concentration

12 µmol/L 16 µmol/L 31 µmol/L

% deviation from baseline concentration

181 IU/L 215 IU/L 313 IU/L

70 600
60
500
50
40 400
30 300
20
200
10
0 100
-10 0
0 1 2 3 4 5 6 7 8 9 10 11 0 1 2 3 4 5 6 7 8 9 10 11
H-Index, g/L H-Index,g/L
 PCT
Lipase
0.5 ng/mL
% deviation from baseline concentration

% deviation from baseline concentration

27 ng/mL 50 ng/mL
20
20
15
15
10
10
5

5 0

0 -5
0 1 2 3 4 5 6 7 8 9 10 11 0 1 2 3 4 5 6 7 8 9 10 11
H-Index,g/L H-Index, g/L

Phosphorus Protein

% deviation from baseline concentration

0.84 mmol/L 1.06 mmol/L 1.24 mmol/L 57 g/L 77 g/L
% deviation from baseline concentration

60 20

50
15
40
30 10
20
10 5
0
0
-10
0 1 2 3 4 5 6 7 8 9 10 11
-5 0 1 2 3 4 5 6 7 8 9 10 11
H-Index, g/L H-Index,g/L