.

Bender MedSystems GmbH

Campus Vienna Biocenter 2
A-1030 Vienna, Austria

Certificate of analysis www.thermofisher.com

Cat. number: LHC0009M Name: Cytokine Human Magnetic 25-

Plex Panel for Luminex™
Platform
Lot number: 280299-001 Expiry Date: 2023-05

Components Quantity Lot Store at

SM311 Hu 16-plex Standard ( dissolve in 500µl ) 2 x each 235435-000 2-8°C
SM310 Hu 14-Plex Standard ( dissolve in 500µl ) 2 x each 226606-000 2-8°C
LM317 Antibody Bead Solution (1x) 1 x 2,5ml 239654-000 2-8°C
BN317 Biotinylated Ab Conc. (10x) 1 x 1ml 248009-000 2-8°C
DA05/2 Assay Diluent 1 x 15ml 268593-000 2-8°C
DP01 Streptavidin-RPE Diluent 1 x 12ml 225399-000 2-8°C
IB03 Incubation Buffer 1 x 12ml 210763000 2-8°C
WB04 Wash Solution Concentrate (20x) 1 x 50ml 211565000 2-8°C
SR08 Streptavidin-RPE Concentrate (10x) 1 x 1ml 268077-000 2-8°C
DB03 Biotin Diluent 1 x 12ml 216935000 2-8°C
SVM104 Black Microplate Lid 1 each 2-8°C
SVM16 Plate Covers 8 each 2-8°C
SVM182 Flat bottom Plate (black) 1 each 2-8°C

Hu 16-plex Standard Lot#235435-000

Provided below is a table of Standard 1 (Std1) value for each analyte in each tube when prepared
according to the "Prepare antigen standard" procedure of the Manual. All data were evaluated in a 2-hour
incubation at room temperature.

Analyte Std1 Concentration ULOQ / LLOQ

(pg/ml) (pg/ml)
Determined in Assay Diluent

GM-CSF 7235 7235 / 9,92

IFN alpha 9725 9725 / 13
IFN gamma 2160 2160 / 2,96
IL-1 beta 10465 10465 / 14
IL-10 13280 13280 / 18
IL-12/IL-23p40 6880 6880 / 9,44
IL-13 10710 10710 / 15
IL-15 18625 18625 / 26
IL-17A (CTLA-8) 22955 22955 / 31
IL-2 15445 15445 / 21
IL-4 39100 39100 / 54
IL-5 11860 3953 / 16
IL-6 12755 12755 / 17
IL-7 17320 17320 / 24
IL-8 (CXCL8) 5790 5790 / 7,94
TNF alpha 4615 4615 / 6,33
.

Hu 14-Plex Standard Lot#226606-000

Provided below is a table of Standard 1 (Std1) value for each analyte in each tube when prepared
according to the "Prepare antigen standard" procedure of the Manual. All data were evaluated in a 2-hour
incubation at room temperature.

Analyte Std1 Concentration ULOQ / LLOQ

(pg/ml) (pg/ml)
Determined in Assay Diluent

EGF 5740 5740 / 7,87

Eotaxin (CCL11) 3700 1233 / 5,08
FGF-2 4865 4865 / 6,67
G-CSF (CSF-3) 56580 18860 / 78
HGF 11475 11475 / 47
IL-1RA 115855 115855 / 159
IL-2R 21280 21280 / 29
IP-10 (CXCL10) 2500 2500 / 3,43
MCP-1 (CCL2) 17150 17150 / 24
MIG (CXCL9) 3350 3350 / 4,60
MIP-1 alpha (CCL3) 15795 15795 / 22
MIP-1 beta (CCL4) 12135 12135 / 17
RANTES (CCL5) 13550 4517 / 19
VEGF-A 2375 792 / 3,26

Antibody Bead Solution (1x) Lot#239654-000

Target Name Bead Number Std1 Concentration Standard

pg/ml

Eotaxin (CCL11) 22 3700 Hu 14-Plex Standard

GM-CSF 27 7235 Hu 16-plex Standard
IFN alpha 43 9725 Hu 16-plex Standard
IFN gamma 38 2160 Hu 16-plex Standard
IL-1 beta 13 10465 Hu 16-plex Standard
IL-10 15 13280 Hu 16-plex Standard
IL-12/IL-23p40 20 6880 Hu 16-plex Standard
IL-13 18 10710 Hu 16-plex Standard
IL-15 30 18625 Hu 16-plex Standard
IL-17A (CTLA-8) 25 22955 Hu 16-plex Standard
IL-1RA 51 115855 Hu 14-Plex Standard
IL-2 54 15445 Hu 16-plex Standard
IL-2R 61 21280 Hu 14-Plex Standard
IL-4 77 39100 Hu 16-plex Standard
IL-5 34 11860 Hu 16-plex Standard
IL-6 19 12755 Hu 16-plex Standard
IL-7 55 17320 Hu 16-plex Standard
IL-8 (CXCL8) 78 5790 Hu 16-plex Standard
IP-10 (CXCL10) 56 2500 Hu 14-Plex Standard
MCP-1 (CCL2) 29 17150 Hu 14-Plex Standard
MIG (CXCL9) 63 3350 Hu 14-Plex Standard
MIP-1 alpha (CCL3) 26 15795 Hu 14-Plex Standard
MIP-1 beta (CCL4) 28 12135 Hu 14-Plex Standard
RANTES (CCL5) 21 13550 Hu 14-Plex Standard
TNF alpha 52 4615 Hu 16-plex Standard
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Analytical information:
This product has been tested by Quality Control and passed internal specifications.

Quality control:

For Research Use Only. Not for use in diagnostic procedures. If you have any further questions about this Certificate of Analysis, please

For inquiries, contact us at "thermofisher.com/askaquestion"