Name: Peligres, Pornela, Portugal, Saito, Tomas, Tulayba Rating:

Course, Year and Section: BSMT2H Instructor:_Prof. Ranola

Group Number: 5 Date:

Tests for Proteins and Amino Acids

EXPERIMENT 7

A. Biuret Test

Sample Result Explanation

Gelatin POSITIVE (+) Gelatin contains at least 2 peptide bonds needed to react with
Purple the Copper sulfate
Egg Albumin POSITIVE (+) Egg albumin contains at least 2 peptide bonds needed to react
Purple with the Copper sulfate
Glycine NEGATIVE (-) It does not have a peptide bond since it is a primary amino
Blue acid

B. Ninhydrin Test

Sample Result Explanation

Egg Albumin Positive (+) The presence of a blue-violet solution indicates the presence
Blue-violet Solution of amino acid in the solution.

Gelatin Positive (+) The presence of a blue-violet solution indicates the presence
Blue-violet Solution of amino acid in the solution.
Alanine Positive (+) The presence of a blue-violet solution indicates the presence
Blue-violet Solution of amino acid in the solution.

C. Hopkins Cole Test

Sample Result Explanation

Albumin Hazy clear solution No purple ring signifies a negative result which means there is
which indicates a an absence of tryptophan-containing proteins.
negative results

Casein slightly hazy white The absence of a purple ring observed between the two layers
solution implies the absence of tryptophan, indicating a negative result.
 Gelatin clear white solution No purple ring which indicates a negative results

Tryptophan yellow solution No appearance of purple ring which indicates a negative results

D. Millon’s Test

Sample Result Explanation

Positive (+) Millon’s test targets the phenol in amino acid; hence, phenol is
Phenol Orange precipitate positive

Negative (-) There is no phenol in Phenylalanine

Phenylalanine Clear

Positive (+) Tyrosine contains phenol side chain; hence, the result is positive
Tyrosine Brick red precipitate

Negative (-) There is no phenol in Casein

Casein Clear

E. SH Test

Sample Result Explanation

Positive (+) Indicates the presence of cysteine, hence, it’s positive

Albumin black precipitate

Negative (-) It doesn’t contain free sulfur in its side chain

Tyrosine no changes in color

Negative (-) It doesn’t contain sulfur-containing amino acid

Casein no changes in color