Color Tests Albumin Casein Gelatin Methioni Cystein Alanine

ne
Positive Positive Positive Negative Negative Negative
Biuret Because the Because the Because the The proteins The proteins The proteins are absent
Proteins are Proteins are Proteins are are absent are absent
present in this test present in this present in this
test test

Positive Positive Negative Negative Negative Negative

Million’s The tyrosine is The tyrosine is Absence of Absence of Absence of Absence of tyrosine or a
present which present which tyrosine or a tyrosine or a tyrosine or a phenol that contain
cause positive cause positive phenol that phenol that phenol that compound
result result contain contain contain
compound compound compound

Positive Positive Negative Negative Negative Negative

Xanthoprotei Presence of Presence of Absence of Absence of Absence of Absence of aromatic
c aromatic amino aromatic amino aromatic aromatic aromatic amino acids
acids acids amino acids amino acids amino acids (tyrosine,tryptophan)
(tyrosine,tryptoph (tyrosine,trypto (tyrosine,tryp (tyrosine,trypt (tyrosine,trypt
an) phan) tophan) ophan) ophan)

Positive Positive Negative Negative Negative Negative

Hopkin’s – Tryptophan is Tryptophan is Tryptophan is Tryptophan is Tryptophan is Tryptophan is absent
Cole present which present which absent which absent which absent which which cause negative
cause positive cause positive cause cause negative cause negative result
result result negative result result
result

Positive Positive Negative Negative Negative Positive

Reduced Sulfur containing Sulfur Sulfur Sulfur Sulfur Sulfur containing amino
Sulfur amino acid is containing containing containing containing acid is present ( cysteine
present ( cysteine amino acid is amino acid is amino acid is amino acid is or cystine)
or cystine) present ( absent absent absent
cysteine or
cystine)

1 Color Reactions of Proteins – WORKSHEET 6

 Negative Negative Positive
Sakaguchi Absence of Absence of Presence of
Test guanidinium guanidinium guanidinium
compound compound compound

Positive Negative Positive Positive Positive Positive

Ninhydrin Proline is present Amino acid Proline is Proline is Proline is Proline is present
Test absent present present present

Glycine Proline Leucine

Color Tests

Biuret

Million’s

Xanthoprotei
c

Hopkin’s –
Cole

Reduced
Sulfur

Sakaguchi
Test

Ninhydrin
Test

Describe the results of each Colour Tests and indicate whether the samples given are
positive or negative in the test samples.

2 Color Reactions of Proteins – WORKSHEET 6

Guide Questions:

1. Why does nitric acid stain the human skin yellow? Do all amino acids give a positive
Xanthoproteic test? Explain.
- nitric acid stain the human skin due to the reactiong of the protein called keratin which we
found in human skin. Yes, it will giove psoitive result because of the aromatic amino acid
which gives a positive result from the amino acid.
2. Which amino acids contain the benzene ring? What is the only acid that contains a phenolic
group? What is the only amino acid that contains the indole group?
Amino acid that contrains benzene ring is phenylalanine, tryptophan and tyrosine. The acid that
contains a phenolic is hydroxybenzoic acid. The acid that contains the indole is trytophan.
3. According to advertisements, a commercial hair dressing contains “protein”. Which of the
following would be the best test to perform on the product to check this claim?
The sulfur test because it is a confirmatory test in keratin and the keratin is a smooths cell
that overlap to form hair strands and its more manageable hair and less frizz.

4. Will a dipeptide give a positive Biuret Test? Explain.

No, because dipeptide does not react on biuret reagent .
5. Indicate whether the following substances would give a positive (+) or negative (-) result with
Biuret Test and give a reason for your choice for every substances given below:
a. Tyrosylphenylalanine
b. Insulin
c. Glycylcysteinyltryptophan
d. Alanylglycine
6. Why would the Ninhydrin Test be more useful and reliable for showing the absence rather
than the presence of proteins? Give the uses of Ninhydrin Test.
- It detects the presence of amino acid in unkniown samples and This test is also used in solid-
phase peptide synthesis to monitor the protection for amino acid analysis of proteins.
Because the Ninhydrin test is ineffective at detecting proteins with a high molecular weight.
7. Give a conclusion of what you learned all throughout this experiment.

3 Color Reactions of Proteins – WORKSHEET 6

 MODULE 6

COLOR REACTION OF PROTEINS

Name: _________________________ Rating: _________________

Date Performed: _________________ Date Submitted: _______________

Experiment No. 6
Data and Report Sheet

4 Color Reactions of Proteins – WORKSHEET 6