Andrea Claire Simpao TISSUE PROCESSING

BSMT3C-1

FIXATION PROCESS

After slicing and separating a tiny piece of heart tissue, it was put in the tissue cassette,
following by formalin was added to complete the fixing procedure. The tissue was then
preserved in a container for 20 minutes. The next tissue surgery will be performed once
we discard the formalin under running water, which will take twenty minutes. One
excellent alternative for fixative application is formalin because of its affordability, ease
of preparation, and accessibility. allergic dermatitis. Staining and analysis will also be
easier because it blocks enzymatic activity and preserves antigenicity. In order to see the
tissue architecture and pathological changes under a microscope and do reliable
histological study and diagnosis, adequate fixation is necessary.

ALCOHOL PROCESS

This step of the tissue processing process involves immersing the tissue in 70% alcohol
for 10 minutes. Ten minutes of discarding resulted in 90% alcohol consumption, followed
by ten minutes of 100% alcohol consumption. This method eliminates water from the
tissue by gradually immersing it in alcohol concentrations that go higher and higher. The
process of dehydration results in the replacement of the water in the tissue with a readily
absorbed material, which is then absorbed by the embedding media. This prepares the
tissue for the following embedding in a supportive medium, such paraffin wax. parts on
future tissue processing simplification and tissue structure preservation
An enough loss of moisture, for example, ensures embedding for microscopic
investigation.
Andrea Claire Simpao TISSUE PROCESSING
BSMT3C-1

CLEARING PROCESS

To make the cleaning process easier, we submerged the tissue specimen in Xylene for ten
minutes. Then, we disposed of the Xylene under running water, then put the wax on. The
process of clearing involves replacing alcohol and other tissue dehydration agents with a
substance that is miscible with both the embedding media and alcohol, such as xylene or
other cleansing agents. This process turns the tissue transparent, which makes it easier
for the embedding fluid to absorb and perfectly preserves the tissue's shape.
Furthermore, cleaning facilitates the removal of any leftover water and facilitates the
subsequent separation of the tissue. Making sure that the tissue has been thoroughly
cleaned before beginning the sectioning, staining, and embedding procedures in
examination of the histopathology.

IMPREGNATION

The impregnation process involves infusing the tissue with a support material, commonly
wax, to give it structural rigidity and permit thin dividing up for microscopic inspection.
The cleaning and tissue dehydration stages are followed by this process. The tissue is
immersed in melted wax or resin during the impregnation process, which allows the
medium to enter the tissue's interstitial spaces and act as a cleaning agent. The tissue
maintains its shape and integrity during sectioning through impregnation, for the aim of
producing superior thin sections that accurately represent the look and pathophysiology
of the tissue under a microscope.