MD - HISCL-800 - Host Specifications - v6.00

HISCL-800
Host Interface Specifications
Revision 6.00
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Table of Contents
1. General ............................................................................................................................................................................................................. 5
2. Scope................................................................................................................................................................................................................ 5
3. History of Revisions ......................................................................................................................................................................................... 5
4. Overview .......................................................................................................................................................................................................... 7
4.1. Terminology .............................................................................................................................................................................................. 7
4.2. Limitations and special remarks ................................................................................................................................................................ 8
5. LIS communication specifications ................................................................................................................................................................... 9
5.1. Communication specifications ................................................................................................................................................................ 10
5.1.1. Connector ......................................................................................................................................................................................... 10
5.1.1.1. Connector .................................................................................................................................................................................. 10
5.1.1.2. Signal identification levels ........................................................................................................................................................ 10
5.1.1.3. Connection cable ....................................................................................................................................................................... 10
5.1.1.4. Interface parameters .................................................................................................................................................................. 11
5.1.2. Data Link Layer (Transmission Protocol) ........................................................................................................................................ 11
5.1.2.1. Communication statuses ............................................................................................................................................................ 11
5.1.2.2. Establishment phase .................................................................................................................................................................. 12
5.1.2.3. Transfer phase ........................................................................................................................................................................... 12
5.1.2.4. Termination phase ..................................................................................................................................................................... 14
5.1.2.5. Timeout ..................................................................................................................................................................................... 14
5.1.3. Presentation Layer ............................................................................................................................................................................ 15
5.1.3.1. Message ..................................................................................................................................................................................... 15
5.1.3.2. Records...................................................................................................................................................................................... 15
5.1.3.3. Fields ......................................................................................................................................................................................... 16
5.1.3.4. Omitting fields........................................................................................................................................................................... 17
5.1.4. Communication protocol .................................................................................................................................................................. 18
5.1.4.1. Analysis information query (HISCL  Host computer) ........................................................................................................... 18
5.1.4.2. Analysis information (Host computer  HISCL) ..................................................................................................................... 18
5.1.4.3. Analysis results (HISCL  Host computer) ............................................................................................................................. 19
5.1.5. Details of records ............................................................................................................................................................................. 20
5.1.5.1. Header record ............................................................................................................................................................................ 20
5.1.5.2. Patient information record ......................................................................................................................................................... 22
5.1.5.3. Query record .............................................................................................................................................................................. 25
5.1.5.4. Analysis order record ................................................................................................................................................................ 28
5.1.5.5. Analysis result record ................................................................................................................................................................ 33
5.1.5.6. Comment record ........................................................................................................................................................................ 38
5.1.5.7. Message termination record....................................................................................................................................................... 39
5.2. Communication Examples ...................................................................................................................................................................... 40
5.2.1. Analysis Information Query (HISCL → Host computer) ................................................................................................................. 40
5.2.1.1. Batch query / Real-time query in sampler mode ....................................................................................................................... 40
5.2.1.2. Batch query / Real-time query in STAT holder mode ............................................................................................................... 40
5.2.2. Analysis Information (Host computer → HISCL)............................................................................................................................ 41
5.2.2.1. Order exists ............................................................................................................................................................................... 41
5.2.2.2. No order exists .......................................................................................................................................................................... 41
5.2.3. Analysis Results Data (HISCL → Host computer) .......................................................................................................................... 42
5.2.3.1. Output of analysis results of a normal sample ........................................................................................................................... 42
5.2.3.2. Output of analysis results of QC sample ................................................................................................................................... 43
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5.3. Network/Interface ................................................................................................................................................................................... 44
5.3.1. Network/Interface............................................................................................................................................................................. 44
5.3.2. IP Address and Port No. ................................................................................................................................................................... 44
5.3.3. Timing of Transmission ................................................................................................................................................................... 44
5.3.4. Transmitted Messages ...................................................................................................................................................................... 44
6. PAMIA and ELSIA Communication Specifications ...................................................................................................................................... 45
6.1. Serial Communication Specifications ..................................................................................................................................................... 45
6.1.1. Protocol Stack .................................................................................................................................................................................. 45
6.1.2. Physical Layer (Hardware) ............................................................................................................................................................... 45
6.1.2.1. Connector .................................................................................................................................................................................. 45
6.1.2.2. Signal identification levels ........................................................................................................................................................ 45
6.1.2.3. Connection cable ....................................................................................................................................................................... 46
6.1.2.4. Communication parameters ....................................................................................................................................................... 46
6.1.2.5. Communication errors ............................................................................................................................................................... 46
6.1.3. Data Link Layer Specifications ........................................................................................................................................................ 47
6.1.3.2. Packet structure ......................................................................................................................................................................... 48
6.1.3.3. Protocol procedure .................................................................................................................................................................... 49
6.1.3.5. Class A status transitions ........................................................................................................................................................... 52
6.1.3.6. Class B status transitions ........................................................................................................................................................... 53
6.1.3.7. Class C status transitions ........................................................................................................................................................... 53
6.1.3.8. Control code reception detection sequence ............................................................................................................................... 54
6.1.4. Session Layer Specifications ............................................................................................................................................................ 55
6.1.4.2. Packet type ................................................................................................................................................................................ 55
6.1.4.3. Protocol procedure .................................................................................................................................................................... 56
6.1.4.4. Relation between transfer interval time and transmission interval time .................................................................................... 56
6.1.4.6. Status transitions........................................................................................................................................................................ 58
6.2. TCP/IP Communication .......................................................................................................................................................................... 59
6.2.1. Protocol Stack .................................................................................................................................................................................. 59
6.2.2. Physical Layer Specifications........................................................................................................................................................... 59
6.2.3. TCP/IP Specifications ...................................................................................................................................................................... 59
6.3. Data Formats ........................................................................................................................................................................................... 60
6.3.1. Parameter Settings by Model............................................................................................................................................................ 60
6.3.1.1. Setting ranges of physical layer ................................................................................................................................................. 60
6.3.1.2. Setting ranges of data link layer ................................................................................................................................................ 61
6.3.1.3. Setting ranges of physical layer ................................................................................................................................................. 62
6.3.2. PAMIA-50 Compatible .................................................................................................................................................................... 63
6.3.2.1. Analysis result format................................................................................................................................................................ 63
6.3.2.2. Analysis order query format ...................................................................................................................................................... 68
6.3.2.3. Instruction format ...................................................................................................................................................................... 71
6.3.3. PAMIA-40i Compatible ................................................................................................................................................................... 74
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6.3.4. ELSIA-FS1200 Special Mode Compatible ...................................................................................................................................... 88
6.3.5. ELSIA-FS1200 Exclusive Mode Compatible................................................................................................................................... 97
7. Special Remarks ............................................................................................................................................................................................. 98
7.1. Order Query Checking ............................................................................................................................................................................ 98
7.2. Order Query Method ............................................................................................................................................................................... 98
7.3. Cautionary Points Regarding Auto Output of Analysis Results .............................................................................................................. 98
7.4. Messages that change due to the HISCL settings .................................................................................................................................... 99
7.4.1. Analysis result format message for over-range results ..................................................................................................................... 99
7.5. Differences between HISCL-800 LIS2 format and HISCL-800 ASTM format .................................................................................... 101
7.6. Differences in the message between the HISCL-800 and other models ................................................................................................ 102
7.6.1. Differences between HISCL-800 LIS2 (ASTM) format and HISCL-5000 LIS2 (ASTM) format ................................................. 103
7.6.2. Differences in PAMIA-50 format of the HISCL-800, HISCL-5000, and HISCL-2000i ................................................................ 103
7.6.3. Differences in the PAMIA-40i formats of the HISCL-800, HISCL-5000, and HISCL-2000i ....................................................... 104
7.6.4. Differences between HISCL-800 LIS2 format and HISCL-2000i ASTM format .......................................................................... 104
7.6.5. Differences between HISCL-800 ASTM format and HISCL-2000i ASTM format ....................................................................... 105
7.6.6. Differences between PAMIA-50 format of the HISCL-800 and PAMIA-50 communication specifications ................................. 106
7.6.7. Differences between PAMIA-40i format of the HISCL-800 and PAMIA-40i communication specifications ............................... 109
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1. General
Automated Immunoassay Analyzer (Chemiluminescent Enzyme Immunoassay Analyzer) HISCL-800
2. Scope
This document describes communication between the HISCL -800 and host computer using LIS/ASTM protocol, and
PAMIA-50/PAMIA-40i/ELSIAFS1200 protocol.
LIS protocol conforms to the “LIS2-A2” standard.
3. History of Revisions
Revision Date Change
1.00 April 10, 2013 Initial version

7.2.1 Analysis result format
6) Rack No.
Deleted description “When the order is from the worklist, “0000” is output.”
because it is unnecessary for the specification.
7) Sample tube position
Deleted description “When the order is from the worklist, the fixed number
“01” is output.” because it is unnecessary for the specification.

6) Rack No.
Deleted description “When the order is from the worklist, “0000” is output.”
2.00 November 07, 2013 because it is unnecessary for the specification.
7) Sample tube position
Deleted description “When the order is from the worklist, the fixed number
“01” is output.” because it is unnecessary for the specification.

7) Rack No.
Deleted description “When the order is from the worklist, “ ”
(4 characters (20h)) is output.” because it is unnecessary for the specification.
8) Cup No.
Deleted description “When the order is from the worklist, spaces (20h) are
output.” because it is unnecessary for the specification.
3.00 December 10, 2013 Changed cover page, header, and footer due to the change of the standard template.
Added examples to 4.2 Restrictions.
Added “The two types of communication methods are available: the serial
communication using RS-232C port and the TCP/IP communication using LAN
port. In this section, the explanation is based on the serial communication.” to
the beginning description in 5. Serial Communication Specifications.
Global change: “IPU” to “HISCL”.
Corrected comment on the list in 5.2.4 Communication parameters to “The

underlined settings conform to the standard.”
3.01 May 12, 2013 Added parameter numbers to the following parameter list:
5.3.1 Communication parameters
5.4.1 Communication parameters
7.2.2. Analysis order query format
4.00 June 27, 2014 Updated the version to 4.00
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In 7.2.2.11 Sample ID in 7.2.2. Analysis order query format,
7.2.5.13 Sample ID in 7.3.2. Analysis order query format, and
7.3.2.3 Sample information (Sample ID) in 7.4.2 Analysis order query format,
added description of how to set whether or not to send the sample ID
“ERRxxxxxx”, which is automatically assigned when a sample barcode
reading error occurs, to the host computer and the description that we do not
recommend the sending of “ERRxxxxxx” to the host computer.
5.00 January 13, 2015
In 7.2.1.9 Rack No. in 7.2.1. Analysis result format, added description of
examples to the Remarks column in the table.
In 7.2.4.27 Instrument ID in 7.3.1 Analysis result format, added description

“The value is left-aligned with space padding.” and changed example in the
Remarks column in the table.
•This combines the “[R314070] HISCL-800 Host Interface Specifications”
(Ver.5.00) and “[R314069] HISCL-800 LIS Interface Specifications”
(Ver.4.00).
6.00 March 24, 2015
•A list of differences due to the HISCL settings and differences from other
models is added to Chapter 7, and these differences are indicated in the body
of this document by notes.
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4. Overview
The purpose of this document is to provide information on communication between the HISCL and host computer using
the LIS2/ASTM protocol, or the PAMIA-50/PAMIA-40i/ELSIAFS1200 protocol in the case of replacement of PAMIA or
ELSIA.
Note:
As a basic rule, LIS2 format is to be used when connecting the HISCL-800 to the host.
For replacement of the HISCL-2000i, it is acceptable to use ASTM format if the host computer is only capable of supporting
the previously used ASTM format.
For PAMIA or ELSIA replacement, it is acceptable to use PAMIA (ELSIA) format if the host computer is only capable of
supporting the previously used PAMIA (ELSIA) format.
*When PAMIA or ELSIA format is used, functional limitations may occur such as inability to communicate patient or other
information between the HISCL and host computer.
*The only differences between LIS2 protocol and ASTM protocol are the maximum allowed number of sample ID digits and
the version number on the header record. For details, refer to section 7.5 of this document.
This document describes the communication specifications for connection of the HISCL-800 to a host computer in the following chapter
structure.
Chapter number Description

Chapter 4 Overview Summary of this document, terms used, and limitations.
Chapter 5 LIS Communication Describes the specifications for communication in LIS2 and ASTM format.
Specifications
Chapter 6 PAMIA and ELSIA Describes the specifications for communication in PAMIA-50, PAMIA-40i,
Communication Specifications ELSIA-FS1200 Special, and ELSIA-FS Exclusive formats.
Chapter 7 Special Remarks Lists messages that change due to the HISCL-800 settings. Also lists differences
from other models in messages.
4.1. Terminology
Terms used in this document are as follows: Characters other than those indicated below cannot be used.
Term Definition
Numeric ASCII codes “0” (30h) through “9” (39h). Full-width characters such as “０” are not treated as
character numeric characters.
Alphabetic ASCII codes “A” (41h) through “Z” (5Ah) and “a” (61h) through “z” (7Ah). Full-width
character characters such as “Ａ” are not treated as alphabetical characters.
Alphanumeric Numeric characters and alphabetic characters.
character
Symbols ASCII codes from the space code (20h, indicated as “” below) through “-” (7Eh), excluding
control characters, numeric characters, and alphabetical characters.
ASCII ASCII codes from spaces (20h, hereinafter described as “”) through “-” (7eh), excluding
control characters.
Multi-language Characters specific to each language, such as hiragana in Japanese. Code pages are used for the
character codes as set in the HISCL settings. Each character uses the amount of area specified
in the code page (In S-JIS, 2 bytes per kanji, etc.).
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4.2. Limitations and special remarks
When a field is not large enough for the HISCL data in communication in a PAMIA-50/PAMIA-40i/ELSIAFS1200 compatible
format, the data is treated as follows:
 Rack number and sample ID:

As many digits as possible are set from the least significant digit, and the higher digits that do not fit are
disregarded.
If there are not enough digits, the Rack No. is right-aligned with zero padding and the Sample ID is right-aligned
with space padding.
[ex] When the Rack No. is “A-1”, “0A-1” is set. (Right-aligned with zero padding)
[ex] When the Sample ID is “Sample-01”, “Sample-01” is set. (Right-aligned with space padding)
 Numerical values such as the concentration:

Digits are discarded from the least significant digit to match the number of digits of the field.
The effective number of digits is as set in the HISCL settings.
When an analysis error, over-range, or other problem occurred, the corresponding characters are output.
For details, refer to 7. Data Formats.
[ex] When the concentration is “123456789.123”, “123456789.1” is set.

[ex] When an analysis error occurred, “---” is output. (Right-aligned with space padding)
If data is defined that cannot be acquired (processed) on the HISCL, the following takes place:
 If the data type is numerical value, the value “0” is used.

 If the data type is text, characters defined as “normal” are used.
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5. LIS communication specifications
Communication specifications are based on a layer protocol.
(1) Physical layer

Specifies transmission and reception of signals between the instrument and the host computer through mechanical and electrical
connections.
(2) Data link layer

Specifies transmission and reception of data in units of frames by link connections between the instrument and the host computer.
(3) Presentation layer

Specifies the messages that are sent and received between the instrument and the host computer.
Presentation layer ← Defines message specifications

Data link layer ← Specifies link connection and frame specifications
Physical layer ← Specifies mechanical and electrical specifications
Note:
The HISCL supports serial and TCP/IP connections.
Standards used for each connection are as follows.
Serial connection TCP/IP connection

Presentation layer LIS2-A2 LIS2-A2
Data link layer LIS2-A2 LIS2-A2
Physical layer LIS2-A2 IEEE802.3
* The IEEE802.3 specifications for the physical layer are not described in this document.
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5.1. Communication specifications
5.1.1. Connector
With the exception of the connector type, the physical layer conforms to the LIS2-A2 standard. The connector type used is a
D-SUB 9-pin male connector.
5.1.1.1. Connector
The D-SUB 9-pin male I/O connector on the back of the HISCL IPU is used.
Table 1: Connector pin assignments

Pin No. Signal name Signal direction
1 NC
2 Reception data RxD IN
3 Transmission data TxD OUT
4 Data terminal ready DTR OUT
5 Signal ground SG -
6 Data set ready DSR IN
7 Request to send RTS OUT
8 Clear to send CTS IN
9 NC
 Do not use control signals. Do not connect pins that are not used.
5.1.1.2. Signal identification levels
Table 2: Signal identification levels

Level Data Signal Control Signal
+3V or higher Logic “0”, start bit ON
-3V or lower Logic “1”, stop bit OFF
5.1.1.3. Connection cable

The HISCL uses a cable with a D-SUB 9 pin female connector, with pin connections as shown below.
HISCL Host computer

DB-9 DB-9 DB-25
TxD 3 3 2 TxD
RxD 2 2 3 RxD
SG 5 5 7 SG
RTS 7 7 4 RTS
CTS 8 8 5 CTS
DTR 4 4 20 DTR
DSR 6 6 6 DSR
NC 1
NC 9
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5.1.1.4. Interface parameters
Table 3: Interface parameter settings
Setting target Setting description
Baud rate 1200 , 2400 , 4800 , 9600 , 14400 , 19200 , 38400 bps
Data length 7 bits , 8 bits
Stop bit 1 bit , 2 bits
Parity None , Even , Odd
* Underlines indicate the default value. (conform to the standard)
5.1.2. Data Link Layer (Transmission Protocol)

The data link layer conforms to the LIS2-A2 standard and message transmission between systems takes place based on the
character-oriented protocol.
This section gives an overview of the communication control procedure. For details, refer to LIS2-A2.
In LIS2-A2, the TCP connection is established prior to communication. In the establishment of the connection,
the host computer acts as a server and the HISCL acts as a client. The HISCL establishes the connection by requesting connection to
the IP address and the port number that are provided by the host computer.
5.1.2.1. Communication statuses

The data link layer has the following two communication statuses:
 Neutral status
 Linked status
Transition to each status is accomplished through the following 3 phases.

(1) Establishment phase
Establishes a communication line, and determines the direction of data transfer. In this way, the sender and the receiver are
identified, and the change is made from neutral status to linked status.
(2) Transfer phase

The sender transmits messages to the receiver until all messages are transferred.
(3) Termination phase

Releases the communication line. Changes both the sender and the receiver from linked status to neutral status.
Establishment phase
Neutral status Linked status Transfer phase
Termination phase
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5.1.2.2. Establishment phase
(1) The sender (HISCL) sends an [ENQ] signal to the receiver (host computer). To respond to the sender, the receiver performs the
following action:
 Returns an [ACK] signal when the communication is enabled.
 Returns a [NAK] signal when the communication is disabled.
If the receiver responds with [NAK] signal, the sender waits for at least 10 seconds before attempting to send another [ENQ]
signal.
Max. 15 sec. Max. 15 sec. At least

E 10 sec. E
E
N N N
HISCL HISCL Q Q
Q
N
A
A
Host computer C
Host computer K
K
(2) When both the sender and receiver send [ENQ] signals, the host computer must yield control authority to the HISCL.
 The HISCL sends [ENQ] signal again after 1 second.
 The host computer must wait for 20 seconds before receiving [ENQ] signal again.
At least
1 sec. E
HISCL N
E E
Q
HISCL N N
Q Q E E
N N
E
Host computer Q Q
N
Host computer At least
Q
20 sec.
5.1.2.3. Transfer phase

During the transfer phase, the sender sends messages to the receiver. The transfer phase continues until all messages have been sent.
(1) Messages are sent in each record with multiple frames. Each frame contains a maximum of 64,000 characters (including frame
overhead). If the record is longer than 63,993 characters, it is divided into two or more frames.
(2) Multiple records cannot be included in a single frame.
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(3) If the record contains the maximum number of characters or less, a frame with the following structure will be transferred.
[STX] [F#] [Text] [ETX] [CHK1] [CHK2] [CR] [LF]
If the record is longer than the maximum number of characters, it is divided into two or more frames. The text termination code
of the intermediate frame is [ETB], and the text termination code of the final frame is [ETX] as shown below.
[STX] [F#] [Text] [ETB] [CHK1] [CHK2] [CR] [LF]

[STX] [F#] [Text] [ETB] [CHK1] [CHK2] [CR] [LF]
……
[STX] [F#] [Text] [ETX] [CHK1] [CHK2] [CR] [LF]
Symbol Description
[STX] Start of frame.
[F#] Frame number.
A numeric character from 0 to 7 is used. The number starts from 1 and goes through 2, 3,
4, 5, 6, 7, 0, after which the sequence repeats.
When resending, the same frame number is sent.
[Text] ASTM E1394-97 records are used.(Refer to 2.3.)
For this reason, the character codes below are not used.
0x00-0x06, 0x08, 0x0A, 0x0E-0x1F, 0x7F, 0xFF
[ETB] Control code indicating end of text (in intermediate frames).
[ETX] Control code indicating end of text (in the final frame).
[CHK1] [CHK2] Expressed by the characters “0”-“9” and “A”-“F.”
The characters from the character immediately after [STX] to [ETB] or [ETX] (including
[ETB] or [ETX]) are added in binary. The least significant 8 bits are expressed as a 2-digit
hexadecimal number, and this is converted into “0”-“9” and “A”-“F” ASCII characters.
The most significant digit is stored in CHK1 and the least significant digit in CHK2.
Example: [STX]3C|A |あｱ[CR] [ETX]
3 C | A (Space) ｜あｱ [CR] [ETX]

33h 43h 7Ch 41h 20h 7Ch 82A0h B1h 0Dh 03h
33h+43h+7Ch+41h+20h+7Ch+82h+A0h+B1h+0Dh+03h = 3B2h
[CHK1] : “B”
[CHK2] : “2”
[CR] [LF] Control code indicating end of frame.
(4) If the receiver has successfully received the frame and is prepared to receive the next frame, it responds with an [ACK] signal.
After the sender receives the [ACK] signal, the sender increments the frame number and either sends a new frame or enters the
termination phase.
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(5) If the receiver fails to receive the frame and is prepared to receive the same frame again, it responds with a [NAK] signal.
After receiving [NAK], the sender resends the last frame using the same frame number.
If the sender fails to send the same frame 6 times consecutively, the sender has to transition to the termination phase to stop
sending the message.
Max. 15 sec. Max. 15 sec.

E
1st attempt 6th attempt O
Sender T
Text frame #1 ••• Text frame #1
N ••• N
A A
Receiver
K K
(6) The HISCL processes an [EOT] response from the host computer as an [ACK] signal. (Response of [EOT] signal from the
receiver is usually a request to suspend a transmission to the sender. However, the HISCL does not support this function.)
5.1.2.4. Termination phase

During the termination phase, the status of both sides returns to neutral.
The sender sends an [EOT] signal to inform the receiver that message transmission has been completed.
By sending an [EOT] signal, the sender changes to neutral status, and by receiving the [EOT] signal, the receiver changes to neutral
status.
5.1.2.5. Timeout
The timer is used to detect a failure to coordinate between the sender and the receiver. The timer is used as a means of recovery from
failure in a communication line or in a communication device.
(1) Establishment phase

The timer is set when the sender sends [ENQ]. A timeout occurs if an [ACK], [NAK], or [ENQ] response is not received within
15 seconds. After the timeout, the sender enters the termination phase.
(2) Transfer phase

The 15-second timer is set when the sender sends the final character of a frame. A timeout occurs if no response is received
within 15 seconds. After the timeout, the sender enters the termination phase.
The receiver sets a 30-second timer when it initially enters the transfer phase or when it responds (by [ACK] or [NAK]) to a
frame. A timeout occurs if the receiver does not receive a frame or [EOT] response from the sender within 30 seconds.
When the receiver detects a timeout, it discards the current incomplete message and enters the termination phase.
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5.1.3. Presentation Layer
5.1.3.1. Message
In the presentation layer, all data is transmitted using messages. A message is composed of record arrays that start with a message
header record (H) and end with a message termination record (L).
5.1.3.2. Records
A record is a sequence of text that begins with an ASCII alphabetic character called the record identifier and ends with [CR].
Table 4: Records
Record
Record type Level Description
identifier
Header H 0 Message sender and receiver information
Patient information P 1 Patient information

A query to the host computer for analysis order
Query Q 1
information
Order record O 2 Analysis order information
Analysis results R 3 Analysis results
Comment record C 1-4 Sample comments or patient comments
Manufacturer information M N/A Not used
Scientific information S N/A Not used
Message termination L 0 Indicates the end of the message.
 A smaller level number indicates a higher level.

 A higher-level record contains information that is common to all lower-level records.
 Levels other than 0 are positioned after higher levels, and the comment record can be inserted at any level.
The comment record is considered to be one lower level than the preceding record. However consecutive comment records are not
allowed.
 Transmission example Correct: H → P → O → R → L Incorrect: H → R → L
(P and O must be transmitted prior to R)
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5.1.3.3. Fields
A record is further divided into fields by field delimiters. A field is identified by its position within a record and has a variable length.
The delimiters indicated below are used.
Table 5: Fields
Delimiter type Code Description
Vertical bar (|)
Field delimiter Field separator in a record.
[7CH]
Back slash (\) Used to repeat the same field when there are multiple components of the
Repeat delimiter
[5CH] same type in one field.
Caret (^)
Component delimiter Separator that separates a field into multiple sub-fields.
[5EH]
Escape delimiter Ampersand (&) Used to embed function characters such as separators and hexadecimal
[26H] in the data as shown below.
[Escape delimiters corresponding to function characters]

(“|” → &F&, “\” → &R&, “^” → &S&, “&” → &E&, hexadecimal →
&Xxxxx&)
However, when multi-language is used for text such as names and

comments, use the byte codes directly without escape delimiters.
Figure 1: Images of Records, Fields, and Sub-fields
Message Record 1 Record 2  Record n
ID RS Field 1 RS Field 2 RS  RS Field n CR
Record identifier Delimiter ‘|’ or ‘\’
Sub-field 1 SS Sub-field 2 SS  SS Sub-field n
Delimiter ‘^’
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5.1.3.4. Omitting fields
If there is no data in the fields subsequent to the field having the data, all subsequent fields can be omitted.
Figure 2: Omitting fields
Delimiter
Records ID RS Field 1 RS Field 2 RS  RS Field n CR
Record identifier
Empty fields
No omission ID RS Field 1 RS RS  RS CR

No value is stored in the empty fields.
Omission ID RS Field 1 CR If the field 2 and subsequent fields are all empty,
they can be omitted.
The fields containing sub-fields store only the sub-field delimiter ‘^’, and the data can be omitted.
It is also possible to omit all content including sub-field delimiters.
Figure 3: Omitting sub-fields

D  D CR
Field Delimiter
Records ID RS Field 1 RS Field 2 RS  RS Field n CR
Record identifier
Sub-field 1 ss Sub-field 2 ss  ss Sub-field n

Sub-field Delimiter
Empty sub-fields
No omission ID RS Field 1 RS Sub-field 1 ss  ss Sub-field n RS  RS Field n CR

For example, if sub-fields in the field 2
Field 2 and subsequent fields are all empty,
they can be omitted as shown below.
No value is stored in the empty sub-fields.
Omission 1 ID RS Field 1 RS ss ss  ss RS  RS CR
Field 2
Omission 2 ID RS Field 1 CR Interpretation is the same for omissions 1 and 2.
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5.1.4. Communication protocol
5.1.4.1. Analysis information query (HISCL  Host computer)

This protocol is used by the HISCL to send a query for analysis information to obtain information about the sample to be analyzed.
Queries can be made using a Sample ID as a keyword, or a Rack Number and Tube Position as a keyword.
Table 6: Analysis Order Inquiry

HISCL Direction Host
ENQ →
← ACK
H : Header record →
← ACK
Q : Query record →
← ACK
L : Message terminator record →
← ACK
EOT →
5.1.4.2. Analysis information (Host computer  HISCL)

This protocol is used by the host computer to respond to a query for analysis information. The comment record can be omitted.
Table 7: Analysis Information

← ENQ
ACK →
← H : Header record
ACK →
← P : Patient information record
ACK →
← C : Patient comment record
ACK →
← O : Analysis order record
ACK →
← C : Sample comment record
ACK →
← L : Message terminator record
ACK →
← EOT
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5.1.4.3. Analysis results (HISCL  Host computer)
This protocol is used for output of the analysis results of routine samples, QC control materials, and calibrators (standard curve
analysis). When outputting QC analysis results and standard curve analysis results, there is no patient information, and thus the data is
transmitted with the patient record left blank. If there are no sample comments or result comments, the Comment record is omitted.
Table 8: Analysis results/QC data

ENQ →
← ACK
H : Header record →
← ACK
P : Patient record →
← ACK
C : Patient comment record →
← ACK
O : Analysis order record →
← ACK
C : Sample comment record →
← ACK
R : Result record
(Transmission is repeated a
→
number of times equal to the
number of parameters)
(Reception is repeated a number of ACK
times equal to the number of (Transmission is repeated a number of
←
parameters) times equal to the number of received
parameters)
C : Result comment record →
← ACK
L : Message termination record →
← ACK
EOT →
19 R314168
5.1.5. Details of records
5.1.5.1. Header record

The details of header record and the examples of transmission/reception are as follows.
Table 9: Details of Header record

HISCL Host computer
Size
Field Field name ↓ ↓ Remarks
(bytes)
Host computer HISCL
7.1.1 Record type H H 1 Fixed value
7.1.2 Delimiter definition |\^& |\^& 4 Fixed value
7.1.3 Message control ID Not used Not used -
7.1.4 Access password Not used Not used -
7.1.5 Sender name or ID Instrument name Not used 9 Sub-fields included.
^Software version ^7
Înstrument serial ^5
number ^^^^8
^^^^PS code
7.1.6 Sender address Not used Not used -
7.1.7 Reserved Not used Not used -
7.1.8 Sender phone number Not used Not used -
7.1.9 Sender characteristics Not used Not used -
7.1.10 Receiver ID Not used Not used -
7.1.11 Comment Not used Not used -
7.1.12 Processing ID Not used Not used -
7.1.13 Version number LIS2-A2 or E1394-97 LIS2-A2 or E1394-97 7 or 8 Fixed value
7.1.14 Date and time YYYYMMDDHH YYYYMMDDHH 14 Not affected by the
MMSS MMSS date format setting.
[Detailed explanation of fields]

1) 7.1.1 Record type
Alphabetic character “H” is set.
2) 7.1.2 Delimiter definition

Alphabetic characters “|\^&” are set.
A delimiter is not needed between 7.1.1 and 7.1.2.
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3) 7.1.5 Sender name or ID
3-1) Instrument name
Alphanumeric characters “HISCL-800” are set. Space padding and zero padding are not performed.
3-2) Software version

The program device version number of the HISCL is set using alphanumeric characters.
Version is 7-byte format of “xxx-yyy”.(“x” and “y” are alphanumeric characters)
When “x” is less than 3 characters, they are right aligned and spaces (20h) are added to the number.
When “y” is less than 3 characters, they are left aligned and spaces (20h) are added to the ending.
[Example]
“00-01” : “00-01” (7 bytes)
“00-01a” : “00-01a” (7 bytes)
“A00-01” : “A00-01” (7 bytes)
3-3) Instrument serial number

The serial number of the HISCL main unit is set using alphanumeric characters.
3-4) PS code
The PS code of the HISCL main unit is set using alphanumeric characters.
Note:
The name and ID values of the transmitting side vary by model. Refer to Chapter 7.
4) 7.1.13 Version number

When the communication format is set to “ASTM” in the HISCL, “E1394-97” (8 bytes) is set.
Other than above, “LIS2-A2” (7 bytes) is set.
Note:
The values used in this format vary depending on whether the HISCL-800 LIS2 format or ASTM format is
selected. If you change the communication format, verify that the values can be accepted by the host. For details,
refer to Chapter 7.
5) 7.1.14 Date and time

The date and time when the message was created is set using numeric characters in the fixed format of
YYYY: Year, MM: Month, DD: Day, HH: Hour (0-23), MM: Minute, and SS: Second.
Example) 13:00:05, Jan. 2, 2015: “20150102130005”
[Example of transmission/reception]
 HISCL → Host computer
H|\^&|||HISCL-800^00-00^12345^^^^12345678||||||||LIS2-A2|20140131000102<CR>
 Host computer → HISCL
H|\^&|||||||||||LIS2-A2|20140131000102<CR>
21 R314168
5.1.5.2. Patient information record
The details of patient information record and the examples of transmission/reception are as follows.
Table 10: Details of Patient information record

HISCL Host computer
Size
(bytes)
Host computer HISCL
8.1.1 Record type P P 1 Fixed value
8.1.2 Sequence number Sequence No. Sequence No. 1-4 1 origin
8.1.3 Doctor assigned Not used Not used -
Patient ID
8.1.4 Laboratory assigned Not used Not used -
Patient ID
8.1.5 Patient ID Patient ID Patient ID 1-16
8.1.6 Patient name ^ Last name ^ Last name ^1-20 Sub-fields included.
^ First name ^ First name ^1-20
8.1.7 Mother’s maiden name Not used Not used -
8.1.8 Date of birth YYYYMMDD YYYYMMDD 8
8.1.9 Patient sex M or F or U M or F or U 1 M: Male, F: Female
U: Unknown
8.1.10 Patient race Not used Not used -
8.1.11 Patient address Not used Not used -
8.1.12 Reserved field Not used Not used -
8.1.13 Patient telephone Not used Not used -
number
8.1.14 Attending physician ID ^Physician name ^Physician name ^1-20 Sub-fields included.
8.1.15 Special field 1 Not used Not used -
8.1.16 Special field 2 Not used Not used -
8.1.17 Patient height Not used Not used -
8.1.18 Patient weight Not used Not used -
8.1.19 Patient’s probable Not used Not used -
diagnosis
8.1.20 Patient active Not used Not used -
medications
8.1.21 Patient’s diet Not used Not used -
8.1.22 Physician field 1 Not used Not used -
8.1.23 Physician field 2 Not used Not used -
8.1.24 Admission and Not used Not used -
discharge dates
8.1.25 Admission status Not used Not used -
8.1.26 Location ^^^Ward ^^^Ward ^^^1-20 Sub-fields included.
8.1.27 DRG or AVG Not used Not used -
8.1.28 DRG or AVG2 Not used Not used -
8.1.29 Patient religion Not used Not used -
8.1.30 Marital status Not used Not used -
8.1.31 Isolation status Not used Not used -
8.1.32 Language Not used Not used -
8.1.33 Hospital service Not used Not used -
8.1.34 Hospital institution Not used Not used -
8.1.35 Dosage category Not used Not used -
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Alphabetic character “P” is set.
2) 8.1.2 Sequence number

Sequence number in numeric characterindicating the order of the appearances of this type of record in the message, starting
from 1. This number is reset to 1 when a higher-level record of the identifier (refer to 5.1.3.2 Records) appears in the message.
Space padding and zero padding are not set.
3) 8.1.5 Patient ID
ID that identifies the patient and for which ASCII characters can be used. Space padding and zero padding are not set.
When service user results are sent, one space (20h) character is output.
In this field, the transmission of spaces only and zero character is prohibited.
Example of patient ID of which the transmission is prohibited
(1) Consisting of spaces only (8 spaces)
P|1||| |^Heisei^Jiro||20010820|M|||||^Dr.2||||||||||||^^ÊAST<CR> (8 spaces)
(2) The number of characters is zero “ ” (zero characters)
P|1||||^Heisei^Jiro||20010820|M|||||^Dr.2||||||||||||^^ÊAST<CR> (zero characters)
4) 8.1.6 Patient name

Patient first name and last name for which ASCII characters and multiple languages can be used.
When service user results are sent, one space (20h) character is output in the first name and in the last name.
The format of “^firstname^lastname” will be applied to non-Japanese.
5) 8.1.8 Date of birth

Date of birth of the patient indicated by numeric characters.
Fixed format of “YYYYMMDD” (YYYY: year, MM: month, DD: day) is used.
When service user analysis results are sent, the fixed value “18000101” is output.
6) 8.1.9 Patient sex

Gender of patient indicated by an alphabetic character and any of M: Male, F: Female, and U: Unknown is set.
7) 8.1.14 Attending physician ID

Name of attending physician for which ASCII characters and multiple languages can be used. Space padding and zero padding
are not set.
8) 8.1.26 Location
Patient’s ward for which ASCII characters and multiple languages can be used. Space padding and zero padding are not set.
 As described in “5.1.3.4” when this record is omitted.
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P|1|||P23456|^Heisei^Taro||20010820|M|||||^Dr.1||||||||||||^^^WEST<CR>
P|1|||100|^Heisei^Jiro||20010820|M|||||^Dr.2||||||||||||^^ÊAST<CR>
P|1||||^△^||||||||^△||||||||||||^^^△<CR>
24 R314168
5.1.5.3. Query record
The details of the inquiry record and the examples of transmission/reception are as follows.
Table 11: Details of Query record

HISCL Host computer
Size
(bytes)
Host computer HISCL
12.1.1 Record type Q Not used 1 Fixed value
12.1.2 Sequence number Sequence No. Not used 1-4 1 origin
12.1.3 Starting range ID Rack No. Not used 6 Sub-fields included.
number ^Rack position ^1 [Size of sample ID]
^Sample ID ^15 or 22 When ASTM is used for the
^Sample ID attribute ^1 communication format: 15
When LIS2 is used for the
communication format: 22
12.1.4 Ending range ID Not used Not used -
number
12.1.5 Universal test ID ^^^Parameter code Not used ^^^1-2 Sub-fields included.
^Parameter name ^1-8 A repeat delimiter ‘\’ (5CH) is
used to specify multiple
Universal test IDs.
12.1.6 Request time range Not used Not used -
12.1.7 Beginning request YYYYMMDDHH Not used 14 Fixed size
results date & time MMSS
12.1.8 Ending request Not used Not used -
results date & time
12.1.9 Requested physician Not used Not used -
name
12.1.10 Requested physician Not used Not used -
telephone number
12.1.11 User field No. 1 Not used Not used -
12.1.13 Requested Not used Not used -
information status
code
Note:
When ASTM is used for the HISCL-800 communication format, the maximum number of sample ID digits is 15. When
LIS2 is used, the maximum number of digits is 22. For details, refer to Chapter 7.

Alphabetic character “Q” is set.

Sequence number in numeric character indicating the order of the appearances of this type of record in the message, starting
from 1. This number is reset to “1” when a higher-level record appears in the message.
3) 12.1.3 Starting range ID number

3-1) Rack number
Number assigned to sample rack, and for which ASCII characters can be used. When the number is less than 6 digits, it
is right-aligned with space padding (20h). When the query is for a sample in the STAT holder or in the work list,
“STAT” is set and the number is right aligned with space padding (20h).
On the HISCL, a front/behind space suppressed number and a front zero suppressed number are treated as a rack
number.
25 R314168
Example: A rack with a barcode of “0xxx” (: one space (20h) character) is recognized as having a rack number
of “xxx”, and text with “xxx” set is sent.
3-2) Rack position

Numeric character indicating the position of the sample in the rack. Fixed at “1” when the query is for a sample in the
STAT holder or in the work list.
Note:
The rack position values vary by model. Refer to Chapter 7.
3-3) Sample ID
Number assigned to the sample using ASCII characters. A maximum of 15 or 22 digits can be used.
If the communication format is set to “LIS2”, the size of this field is 22 bytes. When less than 22 digits, the number is
right aligned with space padding (20h).
If the communication format is set to “ASTM”, the size of this field is 15 bytes. When less than 15 digits, the number is
right aligned with space padding (20h).
On the HISCL, a front/back space-suppressed number is treated as a sample ID.
Example: When the communication format is “LIS2”, a sample tube with a barcode of “1234567890123456” is
recognized as having a sample ID of “1234567890123456”, and text with
“1234567890123456” (22 bytes) set is sent.
Example: When the communication format is “ASTM”, a sample tube with a barcode of “1234”
(: one space (20h) character) is recognized as having a sample ID of “1234”, and text with
“1234” (15 bytes) set is sent.
When the reading of sample ID barcode fails, the HISCL assigns “ERRxxxxxx” using the automatic-assignment function.
Change the following settings to send “ERRxxxxxx” to the host computer. Note that we do not recommend the sending
of “ERRxxxxxx” to the host computer because the sample ID may have an error.
Configure the settings in the file C:\Program Files\Sysmex\IPU\ORG_INI\IPU.ini.

Section: IPU
Parameter: IsErrSmpIDHostSend
Default value of IsErrSmpIDHostSend: 0

When IsErrSmpIDHostSend is 0: Real-time inquiry for sample ID “ERRxxxxx” is not made
When IsErrSmpIDHostSend is 1: Real-time inquiry for sample ID “ERRxxxxx” is made
3-4) Sample ID attribute

An alphabetic character indicates how the sample ID was determined.
26 R314168
Character Definition Description
M Manual entry Used when the sample ID is manually entered (including scanning of
a sample tube barcode label by a hand-held barcode reader).
A Automatic Sample ID automatically assigned by the HISCL. Used when
assignment by the analysis is run with the sample ID not determined and a sample ID
instrument beginning with “Auto-” is assigned.
C Host computer Used when a query to the host is made a second time for a sample
that has a sample ID determined by the host computer.
B Read by ID barcode Used when the sample tube barcode label is read by the instrument’s
reader built-in barcode reader.
4) 12.1.5 Universal test ID

Test parameters that can be analyzed by the HISCL. A repeat delimiter ‘\’ (5CH) is used to specify multiple test parameters.
A maximum of 50 test parameters can be sent depending on the HISCL settings (parameter use settings).
4-1) Parameter code
Alphanumeric characters are output for the code set for each test parameter on the HISCL (Host ID in parameter
information settings) Space padding and zero padding are not set.
Note:
Parameter codes are determined by HISCL setting, and thus may vary by model. For details, refer to Chapter 7.
4-2) Parameter name

ASCII characters are output for the name set for each test parameter on the HISCL (parameter display name in parameter
information settings) Space padding and zero padding are not set.
[ex] When specifying (1) Parameter code: 1, Parameter Name: HBsAg and (2) Parameter code: 10, Parameter Name:HCVAb.
Discribe “^^^1^HBsAg\^^^10^HCVAb”.
5) 12.1.7 Beginning request results date & time

The date and time when the analysis order was required is set using numeric character with the fixed format of
• When the communication format is “LIS2”
Q|1|1^1^Sample##456789012^B||^^^1^HBsAg\^^^10^HCVAb||20150905150000<CR>
• When the communication format is “ASTM”
Q|1|1^1^Sample#123^B||^^^1^HBsAg\^^^10^HCVAb||20150905150000<CR>
Not used
27 R314168
5.1.5.4. Analysis order record
The details of the order record and the examples of transmission/reception are as follows.
Table 12: Details of Analysis order record

HISCL Host computer
Size
(bytes)
Host computer HISCL
9.4.1 Record type O O 1 Fixed value
9.4.2 Sequence number Sequence No. Sequence No. 1-4 1 origin
9.4.3 Specimen ID Not used Rack No. 6 Sub-fields included.
^Rack position ^1 [Size of sample ID]
^Sample ID ^15 or 22 When ASTM is used for the
^Sample ID ^1 communication format: 15
attribute When LIS2 is used for the
9.4.4 Instrument specimen Rack No. Not used 6 communication format: 22
ID ^Rack position ^1
^Sample ID ^15 or 22
^Sample ID attribute ^1
9.4.5 Analysis parameter ID ^^^Parameter code ^^^Parameter ^^^1-3 Sub-fields included.
^Parameter name code ^1-8 A repeat delimiter ‘\’ (5CH) is
^Dilution ratio ^Parameter name ^1-4 used to specify multi-parameter
^Dilution ratio analysis order.
9.4.6 Priority Not used Not used -
9.4.7 Requested/Ordered Not used YYYYMMDD 14
date and time HHMMSS
9.4.8 Specimen collection Not used Not used -
date and time
9.4.9 Collection end Not used Not used -
time
9.4.10 Collection volume Not used Not used -
9.4.11 Collector ID Not used Not used -
9.4.12 Action code N or Q or S N or Q 1 N: Normal sample analysis
Q: QC analysis
S: Standard curve analysis
9.4.13 Danger code Not used Not used -
9.4.14 Relevant clinical Not used Not used -
information
9.4.15 Date/Time specimen Not used Not used -
received
9.4.16 Specimen descriptor Not used Not used -
9.4.17 Ordering physician Not used Not used -
9.4.18 Physician telephone Not used Not used -
number
9.4.21 Laboratory field No. 1 Not used Not used -
9.4.22 Laboratory field No. 2 Not used Not used -
9.4.23 Date/Time results Not used Not used -
reported or last
modified
9.4.24 Instrument charge to Not used Not used -
computer system
9.4.25 Instrument ID Not used Not used -
9.4.26 Report type F Y or Q 1 F: Final result
Y: No order
Q: Response to query
9.4.27 Reserved Not used Not used -
9.4.28 Location or ward of Not used Not used -
specimen collected
9.4.29 Nosocomial infection Not used Not used -
flag
9.4.30 Specimen service Not used Not used -
9.4.31 Specimen institution Not used Not used -
28 R314168
Note:
When ASTM is used for the HISCL-800 communication format, the maximum number of sample ID digits is 15. When LIS2 is
used, the maximum number of digits is 22. For details, refer to Chapter 7.

Alphabetic character “O” is set.

from 1. This number is reset to 1 when a higher-level record appears in the message. Space padding and zero padding are not
set.
3) 9.4.3 Specimen ID
3-1) Rack number
Number assigned to sample rack is output using ASCII characters. The format is the same as the format for this item in
the “Query record”.
The value received from the HISCL must be set.
Recognition of the rack number on the HISCL is the same as for this item in the “Query record”.
3-2) Rack position

Numeric character from 1 to 6 indicating the position of the sample in the rack. The value is the same as for this item in
the “Query record”.
3-3) Sample ID
Number assigned to the sample using ASCII characters. A maximum of 15 or 22 digits are output.
Whether 15 or 22 digits are used and the format of each are the same as for this item in the “Query record”.
Recognition of the sample number on the HISCL is the same as for this item in the “Query record”.

An alphabetic character is output that indicates how the sample ID was determined.
4) 9.4.4 Instrument specimen ID

4-1) Rack number
Number of rack used by HISCL for analysis is set using ASCII characters. The format is the same as the format for this
item in the “Query record”.
4-2) Rack position

Position of sample tube in rack used for analysis indicated by numeric characters. Set to ‘1’ to ‘6’. The value is the same
as for this item in the “Query record”.
29 R314168
4-3) Sample ID
Sample ID used in analysis. In addition to normal sample numbers, automatically assigned numbers, numbers of
QC control materials, and numbers for standard curve analysis are set.
Sample ID Description
Normal sample number 15 or 22 ASCII characters can be used.
Whether 15 or 22 digits are used and the format of each are the same as for this item
in the “Query record”.
Read error number ID number automatically assigned by the HISCL when scanning of the sample
barcode fails. Starts with “ERR”.
Automatically assigned A number that is automatically assigned by the HISCL to an analyzed sample that has
number no sample ID. Starts with “Auto-”. The number of digits depends on the HISCL
settings. Maximum of 15 digits or 22 digits. Numbers only, with the exception of
“Auto-” at the beginning of the sample ID.
QC sample number Special sample number assigned to QC samples as configured in the HISCL settings.
Same as “Normal sample number”.
Standard curve analysis Sample number assigned to results of standard curve analysis. Consists of
number 5 alphanumeric characters, with “STD-” set for the first 4 characters and a numeric
character set for the last character. Right-aligned with space padding to make
15 characters.

An alphanumeric character is output to indicate how the sample ID was determined.
Characters output and their meanings are the same as for this item in the “Query record”.
5) 9.4.5 Analysis parameter ID

5-1) Analysis order instruction
When the host computer instructs the HISCL to perform analysis, the ID of the test to be performed is set. Space padding
and zero padding are not set.
When the analysis order consists of multiple tests, use repeat delimiters ‘\’(5CH).
An analysis order for the HISCL can have a maximum of 24 test parameters. If the HISCL receives an order for more
than 24 test parameters, it performs only the first 24 tests in this field and disregards the remaining tests.
Example: When there are three analysis orders, “^^^Parameter code 1^Parameter name 1^Dilution ratio 1\^^^Parameter code
2^Parameter name 2^Dilution ratio 2\^^^Parameter code 3^Parameter name 3^Dilution ratio 3” is described.
5-2) Parameter code

The alphanumeric code (host ID) set for each test parameter on the HISCL is set. Space padding and zero padding are not set.
Note:
30 R314168
5-3) Parameter name
ASCII characters are set for the parameter names corresponding to the above parameter codes set in the HISCL. Space
padding and zero padding are not set.
5-4) Dilution ratio

“x” of the sample dilution ratio 1/x is set using numeric characters. This must be a dilution ratio that is possible on the
HISCL. Space padding and zero padding are not set.
6) 9.4.7 Date and time of order

The date and time of the analysis order for the queried sample is set using numeric character with the fixed format of
7) 9.4.12 Action code

The sample type is specified using alphanumeric characters. Note that it is not possible to request standard curve analysis on
the HISCL.
Character Description
N Normal sample analysis
Q QC analysis
S Standard curve analysis (No request)
8) 9.4.26 Report type

The content of the record to be sent is specified using alphanumeric characters.
Character Definition Description

F Final result Always this value when analysis results are reported from the HISCL.
Y No order The host computer uses this value when there is no order for a query.
Q Response to query The host computer uses this value when there is an order for a query.
When “Q” is not set, the receiving HISCL assumes that this is
“no order”.
 Same as “5.1.3.4” if this record is omitted due to a reason such as no analysis order for the sample of the received query
(the HISCL is instructed to “not analyze”).
O|1||001^6^78##123456789012^C|^^^1^HBsAg^40\^^^05^HCVAb^1| (next line)
||||||N||||||||||||||F<CR>
O|1||A00001^1^78##12345^C|^^^1^HBsAg^40\^^^05^HCVAb^1|||||||Q| (next line)

|||||||||||||F<CR>

O|1|001^6^78##123456789012^B||^^^1^HBsAg^40\^^^05^HCVAb^1| (next line)
|20010807101000|||||N||||||||||||||Q<CR>
31 R314168
O|1|001^5^△△△△△△78##12345^B||^^^1^HBsAg^40\^^^05^HCVAb^1| (next line)
|20010807101000|||||N||||||||||||||Q<CR>
O|1|STAT^1^78##12345^B||^^^^^|||||||N||||||||||||||Q<CR>
32 R314168
5.1.5.5. Analysis result record
The details of the result record and the examples of transmission/reception are as follows.
Table 13: Details of the Result record

HISCL Host computer
Size
(bytes)
Host computer HISCL
10.1.1 Record type R Not used 1 Fixed value
10.1.2 Sequence number Sequence No. Not used 1-4 1 origin
10.1.3 Analysis parameter ID ^^^Parameter code Not used ^^^1-2 Sub-fields included.
^Parameter name ^3-10 Test parameter name (8 bytes) +
^Dilution ratio ^1-4 Data type (2 bytes)
10.1.4 Data value Result Not used 1-10
10.1.5 Units Units Not used 1-6
10.1.6 Reference range Not used Not used -
10.1.7 Abnormal result flag < or > or N or A or Not used 1 <: Below displayed range
W or X >: Above displayed range
N: Normal
A: Test error, analysis error
W: Low reliability
X: Concentration not
calculated
10.1.8 Nature of Not used Not used -
abnormality testing
10.1.9 Result status Not used Not used -
10.1.10 Date of change of Not used Not used -
instrument normative
values or units
10.1.11 Operator Not used Not used -
identification
10.1.12 Date/Time test Not used Not used -
started
10.1.13 Date/Time test YYYYMMDDHH Not used 14
completed MMSS
10.1.14 Instrument ID Not used Not used -

Alphabetic character “R” is set.

from 1. This number is reset to “1” when a higher-level record appears in the message. Space padding and zero padding are
not set.
3) 10.1.3 Universal test ID

3-1) Parameter code
The alphanumeric code (host ID) set for each test parameter on the HISCL is set. Space padding and zero padding are
not set.
Note:
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3-2) Parameter name
Alphanumeric characters are set for the parameter name corresponding to the above parameter code set on the HISCL,
and the data type that indicates the type of data that is being transmitted.
The parameter name and data type are joined by the alphabetical character “_” (5fh).
Data Type: An alphabetical character indicating the type data being sent is set.
Value Description
F Indicates that the value of the data field is the “concentration” of the analysis results.
Indicates that the value of the data field is the “qualitative evaluation result” of the
I
analysis results.
Indicates that the value of the data field is the “count measured by the instrument” of the
C
analysis results.
3-3) Dilution ratio

The ratio by which the sample was diluted for analysis is set using numeric characters. The numeric character “x” of
the dilution ratio 1/x is set. The dilution ratio of “1”, “40”, or “1600” specified for the analysis is set in “x”. Space
padding and zero padding are not set.
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4) 10.1.4 Data value
Data is set according to the data type of the above “Parameter name” component.
Value Description
F The concentration of the analysis results is set (numeric characters and a period “.” (2eH)). However, if the analysis
results are masked on the HISCL due to an instrument error or other reason, the data value is masked in the same way as
the display as shown below.
Mask Definition Description

**** Four 2aH characters Test results not obtained due to an instrument error.
---- Four 2dH characters Test results not obtained due to an analysis error (incorrect input of
HISCL settings). Also indicates normal completion of standard curve
analysis.
++++ Four 2bH characters Concentration over HISCL upper limit (999,999,999.0) or over test
range of test parameter.
XXXX Four 58H characters Indicates that concentration is not calculated because HISCL has no
standard curve.
 Over-range (over concentration analysis upper limit)

If “Mask over-range result with ‘++++’” is specified in the HISCL settings, “++++” will be output for this value
and “A” will be output in the “Abnormal result flag” described below.
If “Add ‘>’ to over-range result” is specified in the HISCL settings, the analysis upper limit value will be output
for this value and “A” will be output in the “Abnormal result flag” described below.
 Low reliability and outside of display range

For analysis results that have a flag character added on the HISCL, such as results that have a low reliability
result or a concentration outside the display range, a number is output for this value and error information is
output in the “Analysis results error flag” described below.
When this value is a number instead of a mask character, be sure to check the “Analysis results error flag” before
using the value.
I The qualitative evaluation result of the analysis results is set. If a qualitative evaluation is not made or cannot be made
because the analysis results are masked, one space character (20h) is set for this value.
Space (20H) Qualitative evaluation not made
- Negative
+- Pending
+ Positive
1+ Low positive
2+ Medium positive
3+ High positive
1 Group 1 (grouping parameter)
2 Group 2 (grouping parameter)
ND Cannot evaluate (grouping parameter)
IND Evaluation pending (grouping parameter)
RE 2nd dilution analysis recommended (grouping parameter)
C The count value of the analysis results is set. Numeric characters only, commas (2ch) are not used.
Mask Definition Description

0 Numeric character zero Test results not obtained due to an analysis error
(incorrect input of HISCL settings).
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Note:
For the concentration, verify that the host can accept values that are not in exponent format.
For the number of decimal digits of the concentration, verify that the host can accept the number of decimal digits set for
each parameter of the HISCL. For details, refer to Chapter 7.
Note:
The over-range result varies depending on the HISCL settings.
Example: HBsAg upper limit value of 2500

(1) ++++
(2) > 2500
(3) >100000, >4000000 (> upper limit value × dilution ratio)
The above 3 value patterns exist.
Before delivery, check which HISCL setting is to be used. (For the actual contents of the message, refer to 7.4.1.)
5) 10.1.5 Units
Data is set according to the data type of the above “Parameter name” component. Space padding and zero padding are not set.
Data Type Value of this field

F Units set on the HISCL for the corresponding analysis parameter (ASCII character).
I One space (20h) character
C One space (20h) character
Note:
The units of the F sub-field depend on the HISCL-800 settings and vary by parameter. Verify that the host can accept the
different units of each parameter. For details, refer to Chapter 7.
6) 10.1.7 Abnormal result flag

The mask or flag of the concentration in the analysis results is set using alphanumeric characters.
Value of this field Description

< Indicates that the result is below the lower display limit set on the HISCL.
> Indicates that the result is over the upper display limit set on the HISCL.
N Indicates that the results are from analysis that was completed normally without instrument
error.
A Indicates that the analysis results cannot be used due to an analysis error, instrument error,
over-range, or other problem.
W Indicates that a low reliability flag is added to the analysis results due to a slight
abnormality during analysis and the results cannot be used.
X Indicates that the concentration cannot be calculated because there is no standard curve.
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Output cases corresponding to displays on the HISCL are shown below. Check the value in this field before deciding whether the
value in the “Data value” field can be used.
Value of data type “F” of Value of this

HISCL display Meaning of HISCL display
“Data value” field field
*****.*** Test error **** A
-----.--- Analysis error ---- A
Over range /
+++++.+++ ++++ A
Over system upper limit (999,999,999.0)
>123.456 Over range 123.456 A
XXXXX.XXX No standard curve XXXX X
123.456* Low reliability 123.456 W
>123.456 Over upper display limit 123.456 >
<123.456 Under lower display limit 123.456 <
Standard curve analysis results
-----.--- ---- N
(with none of the above errors)
123.456 Other than the above 123.456 N
7) 10.1.13 Date /time test completed

The date and time when the analysis was completed is set using numeric characters with the fixed format of
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5.1.5.6. Comment record
The details of the comment record and the examples of transmission/reception are as follows.
Table 14: Details of Comment record

HISCL Host computer
Size
(bytes)
Host computer HISCL
11.1.1 Record type C C 1 Fixed value
11.1.2 Sequence Sequence No. Sequence No. 1-4 1 origin
number
11.1.3 Comment source Not used Not used -
11.1.4 Comment text Comment Comment Patient comments: 1-100
Order comments: 1-43
Result comments: 1-43
11.1.5 Comment type Not used Not used -

Alphabetic character “C” is set.

from 1. This number is reset to “1” when a higher-level record appears in the message. Space padding and zero padding are
not set.
3) 11.1.4 Comment text

Comment text is set using multiple languages. The maximum length depends on which record the comment is for.
Record Description
Patient Comments on the patient. Multi-language, maximum of 100 bytes.
information When service user analysis results are sent, “ ” (one space (20H) character) is output in this field.
record
Analysis order Comments on the analysis order sample. Multi-language, maximum of 43 bytes.
record When a “Comment ID” is used, the comment ID is alphanumeric 3 bytes and the body is 40 bytes or
less, multi-language.
Here the comment ID consists of two numeric digits (right-aligned with zero padding) and “:” (3aH).
For the comment ID and comment body, use the same information on both the host computer and
the HISCL.
Analysis result Comments on the analysis results. Multi-language, maximum of 43 bytes.
record When a “Comment ID” is used, the comment ID is alphanumeric 3 bytes and the body is 40 bytes or
less, multi-language.
Here the comment ID consists of two numeric digits (right-aligned with zero padding) and “:” (3aH).
For the comment ID and comment body, use the same information on both the host computer and
the HISCL.
C|1||01: Result comments<CR>
C|1||Patient comments<CR>
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5.1.5.7. Message termination record
The details of message termination record and the examples of transmission/reception are as follows.
Table 15: Message terminator record

HISCL Host computer
Size
(bytes)
Host computer HISCL
13.1.1 Record type L L 1 Fixed value
13.1.2 Sequence 1 1 1 Fixed value (Always “1”)
number
13.1.3 Termination N N 1 N: Normal termination
Code

Alphabetic character “L” is set.

Numeric character “1” is set.
3) 13.1.3 Termination Code

Alphabetic character “N” is set.
L|1|N<CR>
L|1|N<CR>
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5.2. Communication Examples
In the following sections, read the communication example as one continuous line even when consisting of two or more
lines.
5.2.1. Analysis Information Query (HISCL → Host computer)
5.2.1.1. Batch query / Real-time query in sampler mode

HISCL <ENQ>
Host <ACK>
HISCL <STX>1H|\^&|||HISCL-800^00-00^11001^^^^12345678||||||||LIS2-A2|
20150324100447<CR><ETX><CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <STX>2Q|1|A-0^5^12345^M|
|^^^1^HBsAg\^^^3^HBsAb\^^^10^HCVAb||
Host <ACK>
HISCL <STX>3L|1|N<CR><ETX><CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <EOT>
5.2.1.2. Batch query / Real-time query in STAT holder mode

HISCL <ENQ>
Host <ACK>
HISCL <STX> 1H|\^&|||HISCL-800^00-00^11001^^^^12345678||||||||LIS2-A2|
Host <ACK>
HISCL <STX> 2Q|1|STAT^1^AntigenQC-High^M||^^^1^HBsAg\^^^3^HBsAb\^^^10^HCVAb||
Host <ACK>
Host <ACK>
HISCL <EOT>
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5.2.2. Analysis Information (Host computer → HISCL)
5.2.2.1. Order exists

Host <ENQ>
HISCL <ACK>
Host <STX>1H|\^&|||||||||||LIS2-A2|20150324100447<CR><ETX><CHK1><CHK2><CR><LF>
HISCL <ACK>
Host <STX>2P|1|||P123456789-23456|^Heisei^Taro||20010820|M|||||^Dr.1|||||||||||
|^^^WEST<CR><ETX><CHK1><CHK2><CR><LF>
HISCL <ACK>
Host <STX>3C|1||Patient comments<CR><ETX><CHK1><CHK2><CR><LF>
HISCL <ACK>
Host <STX>4O|1|A-0^5^123456789012^M|
|^^^1^HBsAg^1000\^^^10^HCVAb^1||20150324100447|||||N||||||||||||||Q<CR><ETX><CHK1><CHK2><C
R><LF>
HISCL <ACK>
Host <STX>5C|1||50:Sample comments NO.50<CR><ETX><CHK1><CHK2><CR><LF>
HISCL <ACK>
Host <STX>6L|1|N<CR><ETX><CHK1><CHK2><CR><LF>
HISCL <ACK>
Host <EOT>
5.2.2.2. No order exists

Host <ENQ>
HISCL <ACK>
Host <STX>1H|\^&|||||||||||LIS2-A2|20150324100447<CR><ETX><CHK1><CHK2><CR><LF>
HISCL <ACK>
Host <STX>2P|1||||^^||||||||^||||||||||||^^^<CR><ETX><CHK1><CHK2><CR><LF>
HISCL <ACK>
Host <STX>3O|1|STAT^1^123456789012^M|
|^^^^^|||||||||||||||||||||Y<CR><ETX><CHK1><CHK2><CR><LF>
HISCL <ACK>
Host <STX>4L|1|N<CR><ETX><CHK1><CHK2><CR><LF>
HISCL <ACK>
Host <EOT>
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5.2.3. Analysis Results Data (HISCL → Host computer)
5.2.3.1. Output of analysis results of a normal sample

HISCL <ENQ>
Host <ACK>
Host <ACK>
HISCL <STX>2P|1|||P123456789-23456|^Heisei^Taro||20010820|M|||||^Dr.1|
|||||||||||^^^WEST<CR><ETX><CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <STX>3C|1||Patient comments<CR><ETX><CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <STX>4O|1||A-0^5^12345^M|
^^^1^HBsAg^1000\^^^2^HBsAb^1\^^^3^HBeAg^1\^^^4^HBeAb^1\^^^5^HBcAb^1\
^^^6^HCVAb^1|||||||N||||||||||||||F<CR><ETX><CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <STX>5C|1||50:Sample comments NO.50<CR><ETX><CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <STX>6R|1|^^^1^HBsAg_F^1600|7.81|IU/mL||N||||||20150324102020<CR>
<ETX><CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <STX>7R|2|^^^1^HBsAg_I^1600||||N||||||20150324102020<CR>
Host <ACK>
HISCL <STX>0R|3|^^^1^HBsAg_C^1600|1234560|||N||||||20150324102020<CR>
Host <ACK>
HISCL <STX>1R|4|^^^2^HBsAb_F^1|XXXX|C.O.I.||X|||||| 20150324102040<CR>
Host <ACK>
(...omitted...)
HISCL <STX>4R|15|^^^5^HBcAb_C^1|----|||A||||||20150324102840<CR>
Host <ACK>
HISCL <STX>5R|16|^^^6^HCVAb_F^1|69.6|C.O.I.||>||||||20150324102900<CR>
Host <ACK>
HISCL <STX>6R|17|^^^6^HCVAb_I^1|3+|||>||||||20150324102900<CR>
Host <ACK>
HISCL <STX>7R|18|^^^6^HCVAb_C^1|1234567890|||>||||||20150324102900
<CR><ETX><CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <STX>0C|1||10: Result comments NO.10<CR><ETX><CHK1><CHK2><CR><LF>
Host <ACK>
Host <ACK>
HISCL <EOT>
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5.2.3.2. Output of analysis results of QC sample
HISCL <ENQ>
Host <ACK>
5.3. 20150324100447<CR><ETX><CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <STX>2P|1<CR><ETX><CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <STX>3O|1||STAT^1^AntibodyQC-Low^M|^^^2^HBsAb^1\
^^^4^HBeAb^1\^^^5^HBcAb^1\^^^6^HCVAb^1|||||||Q||||||||||||||F<CR><ETX>
<CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <STX>4R|1|^^^2^HBsAb_F^1|0.58|mU/uL||N||||||20150324102020<CR><ETX><CHK1>
<CHK2><CR><LF>
Host <ACK>
HISCL <STX>5R|2|^^^2^HBsAb_I^1|-|||N||||||20150324102020<CR><ETX><CHK1>
<CHK2><CR><LF>
Host <ACK>
HISCL <STX>6R|3|^^^2^HBsAb_C^1|12345|||N||||||20150324102020<CR>
Host <ACK>
(...omitted...)
HISCL <STX>7R|12|^^^6^HCVAb_C^1|6789|||N||||||20150324102120<CR>
Host <ETX><CHK1><CHK2><CR><LF>
Host <ACK>
HISCL <EOT>
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5.3. Network/Interface
5.3.1. Network/Interface
HISCL provides the network interface in addition to the serial interface. Conforms to IEEE802.3 and communication is based on
10/100/1000Base-T.
An RJ45 socket is used for the HISCL connection hub and the connection is established so that the communication with the host is
enabled. A UTP Category 5 or higher cable is recommended for the communication cable.
5.3.2. IP Address and Port No.

On the HISCL, TCP/IPv4 is used for IP addresses used in IPU and analyzer communication and the default is as follows.
 IPU : 192.168.37.254
 Analyzer : 192.168.37.253
If you need to change this value, contact your local Sysmex representative.
The IP address of the host computer is set on the HISCL screen. The default TCP port number on the host is 5000, and this number
can be changed.
5.3.3. Timing of Transmission

The HISCL settings can be used to select real-time transmission of the start (analysis order) and completion (result report) of analysis
of single samples, or transmission of batch analysis orders and batch result reports.
5.3.4. Transmitted Messages

Records defined in the presentation layer are transmitted and received based on the protocol of 5.1.2 Data Link Layer (Transmission
Protocol). The TCP connection must be established in order to perform transmission and reception. If not established, the HISCL will
automatically start a session prior to transmission.
For examples of messages that are transmitted and received, refer to 5.2 Communication Examples.
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6. PAMIA and ELSIA Communication Specifications
6.1. Serial Communication Specifications

The two types of communication methods are available: the serial communication using RS -232C port and the TCP/IP
communication using LAN port. In this section, the explanation is based on the serial communication.
6.1.1. Protocol Stack
Application layer Manages application data.
↑↓
Presentation layer Converts application data to packets, and converts packets to application data.
Session layer Request and response synchronized protocol.
Data link layer Sends and receives packets.

 Class A: Non-procedural protocol
 Class B: Procedural protocol
 Class C: Procedural protocol
Physical layer EIA-574 standard
6.1.2. Physical Layer (Hardware)
6.1.2.1. Connector
The D-SUB 9-pin male I/O connector on the back of the HISCL IPU is used.
Pin No. Signal name Signal direction

1 NC -
2 Reception data RxD IN
3 Transmission data TxD OUT
4 Data terminal ready DTR OUT
5 Signal ground SG -
6 Data set ready DSR IN
7 Request to send RTS OUT
8 Clear to send CTS IN
9 NC -
 Control signals are not used. Do not connect pins that are not used.
6.1.2.2. Signal identification levels
Level Data signal Control signal

+3V or higher Logic “0”, start bit ON
-3V or lower Logic “1”, stop bit OFF
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6.1.2.3. Connection cable
The HISCL uses a cable with a D-SUB 9 pin female connector, with pin connections as shown below.
HISCL Host computer

DB-9 DB-9 DB-25
TxD 3 3 2 TxD
RxD 2 2 3 RxD
SG 5 5 7 SG
RTS 7 7 4 RTS
CTS 8 8 5 CTS
DTR 4 4 20 DTR
DSR 6 6 6 DSR
NC 1
NC 9
6.1.2.4. Communication parameters

Parameter Settings
Port COM1, COM2, COM3, COM4
Baud rate 1200, 2400, 4800, 9600, 14400, 19200, 38400 bps
Data length 7 bits , 8 bits
Stop bit 1 bit , 2 bits
Parity None , Even , Odd
* Underlines indicate the default value (conform to the standard)
6.1.2.5. Communication errors

Error Description Protocol action Application action
Off-line DSR or CTS is not active. Wait until this becomes active. Communication error
Framing error Frame error detected. Error reset Communication error
Parity error Parity error detected. Error reset Communication error
Overrun error Overrun error detected. Error reset Communication error
 When a communication error occurs, a dialog box appears and communication stops.
To restore communication, follow the operator instructions.
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6.1.3. Data Link Layer Specifications

No. Parameter Data Range
1 Class Class A, Class B, Class C
2 Transmission interval time 0, 1, 2, 3, 4, 5, 7, 10, 15 (sec)
3 Sum value size 0, 2, 4 (bytes)
4 Retransmission count 2, 3 (times)
5 Packet reception timeout value 3, 5, 60 (sec)
* Should be smaller than packet transmission timeout and
transmission establishment timeout values.
6 Packet transmission timeout value 5, 15 (sec)
7 Transmission establishment timeout value 3, 5 (sec)
8 Reception gap timeout value 5, 60 (sec)
9 Control code format (1) ACK/NAK/ENQ/EOT
(2) STX+ACK+ETX / STX+NAK+ETX /
STX+ENQ+ETX / STX+EOT+ETX
* In the case of (2), the sum value is added after ETX.
(1) Calculation from STX
10 Sum value calculation method
(2) Calculation from next character after STX
* Factory default settings are the same as for the LIS2 protocol.
1. Class:
Data link layer transmission procedure.
2. Transmission interval time:
Interval from end of data packet transmission until transmission of the next data packet.
3. Sum value size:
Size of sum value added to end of packet (bytes).
4. Retransmission count:
Number of packet retransmission attempts. The maximum transmission count is “retransmission count + 1”.
5. Packet reception timeout value:
Time of reception from STX until last code of sum value. Only until ETX when the sum value size is 0 bytes. This timeout
value is also used in control code format (2).
6. Packet transmission timeout value:
Time from end of packet transmission until ACK or NAK is received.
7. Transmission establishment timeout value:
Time from end of ENQ transmission until ACK or NAK is received. Two mutually exclusive values are set on devices that
connect to each other (if device A is 3 seconds, device B to which it connects is 5 seconds). If the same value is set on both
devices, communication cannot take place normally. The device with the smaller value has the stronger transmission right.
8. Reception gap timeout value:
Time from end of packet reception until reception of the next packet (STX) or EOT.
9. Control code format:
Format of control codes used in communication.
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10. Sum value calculation method:
Method used to calculate the sum value.
The scope of application of the communication parameters varies depending on the class as shown below.
Class
No. Class A Class B Class C
Parameter
2 Transmission interval time   
3 Sum value size   
4 Retransmission count ―  
5 Packet reception timeout value   
6 Packet transmission timeout value ―  
7 Transmission establishment timeout value ― ― 
8 Reception gap timeout value ― ― 
9 Control code format ―  
10 Sum value calculation method  ― 
6.1.3.2. Packet structure

(1) Code
All codes used for transmission/reception are ASCII codes.
(2) Packet structure

The packet structure is the same in all classes.
“STX” (02H) is sent at the beginning of the packet, and “ETX” (03H) and the checksum are added at the end of the packet.
Even if the text exceeds 255 characters, the text is sent as a single packet without being divided.
(a) When control code format (1) is used

STX Data packet ETX Checksum
ACK
NAK
ENQ
EOT
(b) When control code format (2) is used

STX Data packet ETX Checksum
STX ACK ETX Checksum
STX NAK ETX Checksum
STX ENQ ETX Checksum
STX EOT ETX Checksum
48 R314168
(3) 4-byte sum value mode
The checksum expresses the ASCII code sum of each byte of the data from STX to ETX (including STX and ETX) in hexadecimal,
converts these to ASCII characters “0” to “9” and “A” to “F”, and stores the characters. The checksum length is 4 bytes.
Example: [STX]A あｱ[ETX]
[STX] A あｱ [ETX]
02H 41H 20H 82A0H B1H 03H = 02H+41H+20H+82H+A0H+B1H+03H = 0239H
4-byte sum value: “0239”

The checksum expresses the ASCII code sum of each byte of the data from the next character after STX to ETX (not including
STX, including ETX) in hexadecimal, converts these to ASCII characters “0” to “9” and “A” to “F”, and stores the characters.
The checksum length is 2 bytes. If the ASCII code sum exceeds 2 bytes, the lowest 2 bytes are used.
Example: [STX]A あｱ[ETX]
[STX] A あｱ [ETX]
(Excluded) 41H 20H 82A0H B1H 03H = 41H+20H+82H+A0H+B1H+03H = 0237H
2-byte sum value: “37”

The sum value size is 0. Therefore, data following ETX does not exist.
(6) Sum check

If a sum value field exists, the sum check is performed on the receiving side.
6.1.3.3. Protocol procedure

There are three protocol classes.
(1) Class A
One-way communication method that does not use control codes when sending packets.
When packets are sent in succession, the transmission interval time is inserted between each transmission. If a sum value field
exists, the sum check is performed on the receiving side.
Sending side Receiving side

Sends packet Sum check
Waits the transmission interval time and Sum check

then sends the next packet.
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(2) Class B
Reciprocal communication whereby a confirmation response (ACK or NAK) is received after a packet is sent.
If the packet is successfully delivered, ACK is returned. If delivery fails, NAK is returned.
When NAK is received, the packet is resent.
When packets are sent in succession, transmission takes place after the transmission interval time following ACK reception.
However, when NAK is received, the next transmission takes place without waiting the transmission interval time.

Sends packet Sum check
Resends when NAK is received If no ERROR, sends ACK.

If an ERROR occurs, sends NAK.
If another packet is to be sent, sends the

packet after the transmission interval.
(3) Class C
This is a protocol that checks delivery of data and contains a process for acquiring the transmission right.
Sends ENQ to acquire the transmission right for data transmission. Reciprocal communication whereby a confirmation response
(ACK or NAK) is received after each transmission.
If two ENQ transmissions collide, each returns to the initial state and ENQ is resent. The resend timings of the HISCL and host
are staggered, with the resend timing of the host taking place more quickly.
The HISCL receives the next ENQ from the host, and from that point, the host acquires the transmission right (the host has the
higher priority transmission right).
After transmission ends, EOT is sent (the transmission right is given up).

Sends ENQ
If ACK, acquires transmission right. Sends ACK to allow other side to

If NAK or a timeout occurs, resends. send, otherwise sends NAK.
Sends packet (repeated N times)

If NAK or a timeout occurs, resends.
If sum check is abnormal, sends

NAK.
If normal, sends ACK.
When packet transmission is completed,

sends EOT.
(Yields transmission)
Yields reception
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(1) Class A sending side

Protocol Application
Error Description
action action
(None)
(2) Class A receiving side

Error Description
action action
Receives packet timeout The last sum value code is not received within the Ends reception Communication
specified time after STX reception (until ETX if phase. error
there is no sum value).
Sum check error Sum check error detected after packet reception No action Communication
error
(3) Class B sending side

Error Description
action action
Sends packet timeout Specified time elapsed without receiving ACK or Ends Communication
NAK after packet transmission. transmission error
phase.
(No resending)
Over resend count Resend count exceeded during packet resending. Ends Communication
A packet is only resent when NAK is received, not transmission error
when a packet transmission timeout occurs. phase.
(4) Class B receiving side

Error Description
action action
Sum check error Sum check error detected after packet reception Sends NAK Communication
error
Receives packet timeout The last sum value code is not received within the Ends reception Communication
specified time after STX reception (until ETX if phase. error
(5) Class C sending side

Error Description
action action
Sends packet Specified time elapsed without receiving ACK or Resends packet Communication
timeout NAK after packet transmission. error
Transmission Specified time elapsed without receiving ACK or Resends ENQ Communication
establishment timeout NAK after ENQ transmission. error
Over resend count Retry count exceeded during packet resending or Ends Communication
ENQ resending. A packet is resent when NAK is transmission error
received or a packet transmission timeout occurs. phase.
ENQ is resent when NAK is received or a
transmission establishment timeout occurs.
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(6) Class C receiving side
Error Description
action action
Sum check error Sum check error detected after packet reception Sends NAK Communication
error
Reception gap timeout The next packet (STX) or EOT is not received Ends reception Communication
within the specified time after packet reception. phase. error
Packet reception The last sum value code is not received within the Ends reception Communication
timeout specified time after STX reception (until ETX if phase. error
6.1.3.5. Class A status transitions
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6.1.3.6. Class B status transitions
6.1.3.7. Class C status transitions
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6.1.3.8. Control code reception detection sequence
This is applied when an ACK, NAK, ENQ, or EOT control code is received.
When a timeout occurs in any of the waiting states indicated below, the status transition for that state takes place.
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6.1.4. Session Layer Specifications

Parameter Data range
Transfer interval 0, 1, 2, 3, 5, 7, 10, 15 (sec)
Host response timeout value 5 to 30, 60 (sec) Default 15 sec.
Retransmission count 0 (times)
[Additional descriptions of parameters]

(1) Transfer interval
Interval from reception of the response to one request until transmission of the next request (or data with no response).
In TCP/IP, transfer interval = 0 only.
(2) Host response timeout

Wait time for a response from the host after the HISCL sends a request.
(3) Retransmission count

Request retransmission count. Retransmission is not performed in the session layer.
6.1.4.2. Packet type
There are three types of packets in the session layer.
(1) Request packet

When this packet is sent, a response packet is returned.
(2) Response packet

Response to a request packet.
(3) Notification packet

One-way notification packet that notifies the receiving side of data. A response is not returned.
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6.1.4.3. Protocol procedure
When a request is sent, a response is returned. In this system, the HISCL is the requesting side and the host is the responding side.
The starting point for the transfer interval is reception of a response. The starting point for the transfer interval in a notification packet
is notification transmission.
An order query is sent based on this protocol, and the response message is checked against the request message. For details, refer to
“Special Remarks”.
(1) Request and response

HISCL (requesting side) Host (responding side)
Sends request Class transmission Sends request
Receives response Class transmission Sends response

If there is another request, waits the
transfer interval time and then sends
the request.
(2) Notification
Notification Class transmission

If there is another packet, waits the
transfer interval time and then sends
the packet.
Sends request (or notification) Class transmission Receives request (or notification)
Class transmission
Receives response Sends response
6.1.4.4. Relation between transfer interval time and transmission interval time
The relation between the transfer interval time of the session layer and the transmission interval time of the data link layer is explained
below.
The starting points of each are as follows.
 The starting point of the transfer interval time is the reception of a response.
 The starting point of the transmission interval time is transmission of a request (Class A)
Reception of ACK (Class B)
Reception of EOT (Class C)
* The starting point of the host response timeout time is the same as the transmission interval time.
Therefore, a time axis exists on which the transfer interval time and transmission interval time operate simultaneously. To send the
next transmission in this type of situation, you must wait until both time out. In other words, transmission to the physical layer is not
possible until both the data link layer and session layer change to the idle state.
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The relation between the transfer interval time and transmission interval time is explained below using Class B as an example.
(1) When transmission interval end time < transfer interval end time
Sends request
ACK
Transmission interval starts
Response
Transfer interval starts
ACK
timeout
Waits the transfer interval time and sends
ACK
timeout
(2) When transmission interval end time > transfer interval end time
Sends request
ACK
Transmission interval starts
Response
Transfer interval starts
ACK
timeout
Waits until the transmission interval time
elapses and then sends
ACK
timeout
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Error Description
action action
Host response timeout After a request is sent from the HISCL, a response Ends Communication
is not received from the host within the specified transmission error
time. phase.
(No resending)
6.1.4.6. Status transitions
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6.2. TCP/IP Communication
6.2.1. Protocol Stack
Application layer Manages application data.

↑↓
Presentation layer Converts application data to packets, and converts packets to application data.
Session layer Request and response synchronized protocol.
Sends and receives packets.
Data link layer  Class A: Non-procedural protocol

 Class B: Procedural protocol
 Class C: Procedural protocol
One transmission/reception connection is used to perform data link layer class

TCP
transmission within that connection.
IP Manages IP addresses and performs routing control.
Physical layer (Ethernet) Conforms to IEEE802.3.
For details on the presentation layer, refer to the following “Data Format” chapter. For the specifications of the data link layer and
session layer, refer to the previous “Serial Communication” chapter.
6.2.2. Physical Layer Specifications

 Conforms to IEEE802.3.
 Communication is based on 10/100/1000Base-T.
 An RJ45 socket is used to connect the HISCL.
 A UTP Category 5 or higher cable is used for the communication cable.
6.2.3. TCP/IP Specifications

One connection is used to perform data link layer class transmission within that connection.

Protocol
Error Description Application action
action
Connection error Connection broken due to a circuit break or Restore connection Communication
other problem. error
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6.3. Data Formats
Data formats are as follows:
 Analysis results
 Order query
Communicates by pairing with instruction responses.
 Instruction response
Communicates by pairing with order queries.
Data formats also exist for each model.
6.3.1. Parameter Settings by Model

The setting ranges of parameters that can be set are indicated below.
6.3.1.1. Setting ranges of physical layer

Physical layer
Data format RS-232C
Ethernet data
port baudrate stopbit parity
length
600, 1200, 2400, 4800, 9600,
PAMIA-50  COM1, 2 ,3, 4 7, 8 1, 2 none, even, odd
14400, 19200, 38400
600, 1200, 2400, 4800, 9600,
PAMIA-40i  COM1, 2 ,3, 4 7, 8 1, 2 none, even, odd
14400, 19200, 38400
ELSIA-FS1200 600, 1200, 2400, 4800, 9600,
 COM1, 2 ,3, 4 7, 8 1, 2 none, even, odd
Special mode 14400, 19200, 38400
ELSIA-FS1200 600, 1200, 2400, 4800, 9600,
 COM1, 2 ,3, 4 7, 8 1, 2 none, even, odd
Exclusive mode 14400, 19200, 38400
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6.3.1.2. Setting ranges of data link layer
Data link layer
Model Transmission Sum size Retransmission Packet reception
Class
interval time (sec) (bytes) count timeout (sec)
A 0, 2, 3, 5, 7, 10, 15 0 ― 3, 5, 60
PAMIA-50
B 0, 2, 3, 5, 7, 10, 15 0 3 3, 5, 60
A 0, 2, 3, 5, 7, 10, 15 0 ― 3, 5, 60
PAMIA-40i
B 0, 2, 3, 5, 7, 10, 15 0 3 3, 5, 60
ELSIA-FS1200 A 0, 1, 2, 3, 4, 5 0, 4 ― 3, 5, 60
Special mode C 0, 1, 2, 3, 4, 5 0, 4 2 3, 5, 60
ELSIA-FS1200 A 0, 1, 2, 3, 4, 5 2 ― 3, 5, 60
Exclusive mode C 0, 1, 2, 3, 4, 5 2 2 3, 5, 60
Data link layer

Packet
Model Transmission Reception
transmission
Class establishment gap timeout Control code format
timeout
timeout (sec) (sec)
(sec)
A ― ― ― ―
PAMIA-50 (1) ACK/NAK or
B 15 ― ― (2) STX + ACK + ETX /
STX + NAK + ETX
A ― ― ― ―
PAMIA-40i (1) ACK/NAK or
B 15 ― ― (2) STX + ACK + ETX /
STX + NAK + ETX
A ― ― ― ―
ELSIA-FS1200
Special mode 3(HOST)
C 5, 15 5, 60 (1) ACK / NAK / ENQ / EOT
5(HISCL)
A ― ― ― ―
ELSIA-FS1200
Exclusive mode 3(HOST)
C 5, 15 5, 60 (1) ACK / NAK / ENQ / EOT
5(HISCL)
Data link layer

Model
Class Sum value calculation method
A ―
PAMIA-50
B ―
A ―
PAMIA-40i
B ―
ELSIA-FS1200 A (1) Calculation from STX
Special mode C (1) Calculation from STX
(2) Calculation from next
ELSIA-FS1200 A
character after STX
Exclusive mode
(2) Calculation from next
C
character after STX
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6.3.1.3. Setting ranges of physical layer
Session layer
Communication
Model Host response Retransmission
methods Transfer interval
timeout count
Serial 0, 2, 3, 5, 7, 10, 15 60 0
PAMIA-50
Tcp/Ip 0 60 0
Serial 0, 2, 3, 5, 7, 10, 15 60 0
PAMIA-40i
Tcp/Ip 0 60 0
ELSIA-FS1200 Serial 0, 1, 2, 3, 5, 7, 10, 15 5-30 0
Special mode Tcp/Ip 0 5-30 0
ELSIA-FS1200 Serial 0, 1, 2, 3, 5, 7, 10, 15 5-30 0
Exclusive mode Tcp/Ip 0 5-30 0
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6.3.2. PAMIA-50 Compatible
6.3.2.1. Analysis result format

The text in brackets in the parameter column means parameter number.
(1) Sample ID subtext

No. Parameter LENGTH Remarks
7.2.1.1 Text distinction code I 1 “D” (fixed)
7.2.1.2 Text distinction code II 1 “S” (fixed)
7.2.1.3 Text distinction code III 2 “31” (fixed)
7.2.1.4 Block number 2 “01” (fixed)
7.2.1.5 Total number of blocks 2 “02”-“25”
7.2.1.6 Sample distinction code 1 “U”, “E”, “S”, “C”
7.2.1.7 Date 6 YYMMDD, MMDDYY, DDMMYY
7.2.1.8 Time 4 HHMM
7.2.1.9 Rack No. 4 Example: “0A-1” Rack No. “A-1”
7.2.1.10 Sample tube position 2 Example: “06”, Sample tube position “6”
7.2.1.11 Sample ID 13 Example: “Sample-01”, Sample ID “Sample-01”
7.2.1.22 Reserved 12 “”
Total 50
(2) Concentration subtext

7.2.1.4 Block number 2 “02”-“25”
7.2.1.12 Parameter code 3 Example: “001”, Host ID “01”
7.2.1.13 Concentration 11 Example: “>123.45”, “9999999.999”,
“---”
7.2.1.14 P/T(T1) 6 “0.00” (fixed)
7.2.1.15 P/T(T2) 6 “0.00” (fixed)
7.2.1.16 Analysis status (T1) 8 “********” (fixed)
7.2.1.17 Analysis status (T2) 8 “O*E**X+-”
7.2.1.18 Dilution ratio 5 Example: “40”, analysis with 1/40 dilution
7.2.1.19 Repeat analysis dilution ratio 5 Example: “1600”, repeat analysis with 1/1600 dilution
7.2.1.20 Repeat analysis 1 “0” (initial test), “1” (repeat test)
7.2.1.21 Reaction time 1 “2” (fixed)
Total 62
This format consists of a combination of sample ID subtexts that hold sample control information such as the sample ID, and
concentration subtexts that hold the concentration of each analysis parameter.
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Subtext combinations are as follows.
 Result of 1st parameter analysis: Sample ID subtext - Concentration subtext (end)
 Result of 2nd parameter analysis: Sample ID subtext - Concentration subtext of parameter 1 - Concentration subtext of
parameter 2 (end)
[Additional descriptions of parameters]

1) Order of output
The order of output is from the top parameter down, from the most significant digit of the data to the least significant digit.
2) 7.2.1.1, 7.2.1.2, 7.2.1.3 Text distinction codes I to III

Fixed characters are sent that indicate that the format is the analysis data text format of the PAMIA-50 format.
3) 7.2.1.4, 7.2.1.5 Block number and total number of blocks

The total number of blocks of the sample ID subtext and concentration subtext, and the position within the total number. If there
are not enough digits in the date or time, the data is right-aligned with zero padding.
4) 7.2.1.6 Sample distinction code

A character is output that indicates the sample type.
U Routine sample
E Urgent sample (applies to all sample types analyzed in the STAT holder)
S Sample for standard curve analysis
C Sample for QC analysis
5) 7.2.1.7, 7.2.1.8 Date and time

The date and time of analysis is output. The date format is as set in the HISCL settings. If there are not enough digits in the date
or time, the data is right-aligned with zero padding. The least significant two digits of the year are output.
6) 7.2.1.9 Rack No.

Sample rack number consisting of 4 ASCII characters. Spaces (2dh) and other symbols are included in the number of digits of
the rack number. If there are not enough digits, the data is right-aligned with zero padding. When the STAT holder is used,
“0000” is output.
If the number is over 4 digits, the most significant digits are disregarded and only the lowest 4 digits are output. If alphabetical
characters or symbols are used in the rack number on the HISCL, those characters or symbols are output.
7) 7.2.1.10 Sample tube position

Indicates the position of the sample in the sample rack. “01” to “06” for a rack, or fixed at “01” for a sample in the STAT holder.
When the order is from the worklist, the fixed number “01” is output.
If there are not enough digits, the data is right-aligned with zero padding.
Note:
The sample tube position values vary by model. Refer to Chapter 7.
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8) 7.2.1.11 Sample ID
The sample ID is 13 ASCII characters. Spaces (2dh) and other symbols are included in the number of digits of the sample ID.
If there are not enough digits, the number is right-aligned with space padding.
If the number is over 13 digits, the most significant digits are disregarded and only the lowest 13 digits are output.
If alphabetical characters or symbols are used in the sample ID on the HISCL, those characters or symbols are output.
Note:
The sample ID values vary by model. Refer to Chapter 7.
9) 7.2.1.12 Parameter Code

The host ID alphanumeric code of the test parameter set on the HISCL is output.
Note:
10) 7.2.1.13 Concentration

The concentration is sent with a decimal point in the format displayed on the HISCL. Output in exponent format is not possible.
If the concentration is masked on the HISCL due to an analysis error, over-range, or other problem, “---” is
output.
In this event, characters corresponding to the “Analysis status” described below are output. Check the analysis status before
using the concentration that was output.
The masks and flags displayed on the HISCL correspond as shown below to the characters output to the host.
Analysis status HISCL display Characters output

Mask Test error *****.*** “---”
Analysis error -----.--- “---”
Over range /
+++++.+++ “---”
Over range >123.456 “>123.456”
No standard curve XXXXX.XXX “---”
Flag Low reliability 123.456* “123.456”
Over upper display limit >123.456 “>123.456”
Under lower display limit <123.456 “<123.456”
 A low reliability flag is not output in this field. Check the value of the “Analysis status” described below before deciding
whether the value in this field can be used.
 For “over range”, if “Mask over range results with ‘++++’” is specified in the HISCL settings and the measured value is over
the upper analysis limit, “---” is output and “O” is output in the analysis status (T2).
If “Add ‘>’ to over-range results” is specified and the measured value is over the upper analysis limit, “>” and the upper
analysis limit value are output in this field, and “O” is output in the analysis status (T2).
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Note:

(1) ++++
(2) > 2500
Note:
The concentration values vary by model. Refer to Chapter 7.
11) 7.2.1.14, 7.2.1.15 P/T

“0.00” is output for both T1 and T2, right-aligned with space padding.
Note:
The value of this field does not exist on the HISCL-800, so T1 and T2 are both fixed at “0.00”.
12) 7.2.1.16, 7.2.1.17 Analysis status

The status (such as an analysis error) and qualitative evaluation result are output as characters.
<T1>
“” is always output.
Note:
On the HISCL-800, this field is always fixed at “**********” and cannot be used. For details, refer to Chapter 7.
<T2>
When there is no error and a qualitative evaluation is not made, “” is output.
When there is an error or a qualitative evaluation is made, characters other than “” are output in the corresponding positions in
the format “OEX+-”.
The information displayed on the HISCL corresponds as shown below to the characters that are output.
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Characters
Parameter HISCL display
output
Error Over upper limit of analysis range (over range) O
Mask : Over range
Analysis error E
Mask : Test error, analysis error
Flag : Low reliability
No standard curve X
Mask : No standard curve
Qualitative Negative (-) “-”
evaluation Pending (+-) “+-”
Positive (+) “+”
Low positive (1+) “1+”
Medium positive (2+) “2+”
High positive (3+) “3+”
Grouping parameter : Group 1 (1) “1”
Grouping parameter : Unable to evaluate (ND) “ND”
Grouping parameter : Pending (IND) “IN”
Grouping parameter : 2nd dilution analysis recommended (RE) “RE”
Note:
The HISCL-800 has more qualitative judgment items than the PAMIA-50. Ensure that the host can accept these items.
For details, refer to Chapter 7.
13) 7.2.1.18 Dilution ratio

The sample dilution ratio used for analysis is output. One of the following is output, right-aligned with space padding.
“1”, “40”, “1600”
Note:
On the HISCL-800, values other than 1, 40, and 1600 are not transmitted. For details, refer to Chapter 7.
14) 7.2.1.19 Repeat analysis dilution ratio

Outputs the retest dilution ratio when a retest order is created on the HISCL. The content output is the same as for the dilution
ratio above.
If a retest order has not been created, “0” is output, right-aligned with space padding.
15) 7.2.1.20 Repeat analysis

A number is output to indicate initial analysis or repeat analysis.
“0” is output for initial analysis. “1” is output for repeat analysis.
16) 7.2.1.21 Reaction time

Fixed at “2”
17) 7.2.1.22 Reserved

Reserved for future use. Currently all are set to spaces (20h).
Do not perform a data check on this data.
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6.3.2.2. Analysis order query format

7.2.2.1 Text distinction code I 1 “R” (fixed)
7.2.2.2 Text distinction code II 1 “R” or “I”
7.2.2.4 Block number 2 “01”
7.2.2.5 Total number of blocks 2 “01”
7.2.2.6 Sample distinction code 1 “”
7.2.2.8 Time 4 HHMM
7.2.2.10 Sample tube position 2 Example: “06”
Example: “”,
7.2.2.11 Sample ID 13
“Sample-01”
7.2.2.12 Reserved 1 “”
7.2.2.13 Type code 3 Example: “000”, “001”, “999”
7.2.2.14 Number of parameters 2 Example: “01”, “12”
7.2.2.15 Parameter code 1 3 Example: “001” Host ID “01”

Parameter code N 3 Example: “099” Host ID “99”
Total 44+3×N
This format is used to send the sample position, sample ID, and parameters that can be analyzed on the HISCL to the host computer.
A query for one sample is sent from the HISCL to the host computer using one query packet, and an analysis instruction is sent from
the host computer to the HISCL using one instruction packet.
Note:
If the number of items increases, the overall message length of the specified format will increase. Verify that the host can handle the
increased length.
[Detailed descriptions of parameters]

1) Order of output
The order of output is from the top parameter down, with the data from the most significant digit to the least significant digit.

Fixed characters are sent that indicate that the format is the query text format of the PAMIA-50 format.
Query whereby the rack number and sample tube position are used as
R
keywords and the sample ID is left blank.
Query whereby the sample ID is used as a keyword rather than being left
I (alphabetical character “I”)
blank.

The total number of blocks and the position within the total number.
A query consists of one text block, so these are always “01”.
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Always spaces (20h).
5) 7.2.2.7, 7.2.2.8 Date and time

The date and time of the query is output. The date format is as set in the HISCL settings. If there are not enough digits in the
date or time, the data is right-aligned with zero padding. The least significant two digits of the year are output.
6) 7.2.2.9 Rack No.

the rack number. If there are not enough digits, the data is right-aligned with zero padding.
When the STAT holder is used, “0000” is output. When the order is from the worklist, “0000” is output.
If alphabetical characters, symbols, or spaces are used in the rack number on the HISCL, those characters/symbols/spaces (20 h)
are output.
Even if the query uses a sample ID as a keyword as described above, the corresponding rack number is output.

When the order is from the worklist, the fixed number “01” is output.
Even if the query uses a sample ID as a keyword as described above, the corresponding sample tube position is output.
Note:
8) 7.2.2.11 Sample ID
If a query is made without specifying a sample ID, spaces “” are output (13 characters (20h)).
Even if the query uses a rack number and sample tube position as keywords as described above, the sample ID corresponding to
the rack number and sample tube position is output.
Change the following settings to send “ERRxxxxxx” to the host computer. Note that we do not recommend the sending of
“ERRxxxxxx” to the host computer because the sample ID may have an error.

Section: IPU

When IsErrSmpIDHostSend is 0: Real-time inquiry for sample ID “ERRxxxxx” is not made
(the same action as performed in Ver. 00-17 and 00-18).
When IsErrSmpIDHostSend is 1: Real-time inquiry for sample ID “ERRxxxxx” is made
(the same action as performed in Ver. 00-16 and prior).
Note:
The sample ID values vary by model. Refer to Chapter 7
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9) 7.2.2.12 Reserved
Reserved for future use. Currently all are set to spaces (20h). Do not perform a data check on this data.
10) 7.2.2.13 Type code

The desired order type that is sent to the host computer is output.
In a batch query, the number specified in the query is output, right-aligned with zero padding.
In a real-time query, “000” is output.
Decide the number that indicates the order type so that it corresponds with the host computer.
11) 7.2.2.14 Number of analysis parameters

The number of parameters that can be analyzed based on the current HISCL settings is output, right-aligned with zero padding.
The number of parameters that can be analyzed is as set in the HISCL settings (parameter usage settings), with the maximum
number being “50”.
12) 7.2.2.15 Parameter code 1 to N

The host ID of each analysis parameter that can be analyzed on the HISCL is output as right-aligned alphanumeric characters
with zero padding.
The host IDs are determined by the HISCL settings.
A maximum of 50 analysis parameters are output as set in the HISCL settings (parameter usage settings).
Note:
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6.3.2.3. Instruction format

7.2.3.1 Text distinction code I 1 “S” (fixed)
7.2.3.2 Text distinction code II 1 “0” (fixed)
7.2.3.3. Text distinction code III 2 “31” (fixed)
7.2.3.8 Time 4 HHMM
Example: “”,
7.2.3.11 Sample ID 13
“Sample-01”
7.2.3.12 Reserved 12 “”
7.2.3.14 Dilution ratio (parameter 1) 5 Example: “1”, analysis with x1 dilution
7.2.3.15 Reserved 2 “”

Dilution ratio (parameter N) 5 “ 1600”, analysis with 1/1600 dilution
Reserved 2 “”
Total 50+10×N
The host computer uses this format to specify analysis parameters and dilution ratios to the HISCL in response to a query for a sample.
The count for multiplex analysis is fixed at 1 in this instruction. In order to perform duplex analysis, this must be specified in the
HISCL settings in advance (host supplemental rack setting).
A query is made for one sample using one query packet, and an analysis instruction is returned using one instruction packet.
Note:
increased length.
[Detailed descriptions of parameters]

1) Order of output

Fixed characters are sent that indicate that the format is the instruction text format of the PAMIA-50 format.
“0” in text distinction code II is the number zero.

If there are not enough digits in the date or time, the data is right-aligned with zero padding.
The instruction text consists of one text block, and thus both are always “01”.

Always spaces (20h).
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5) 7.2.3.7, 7.2.3.8 Date and time
The date and time of the query is output. The date format is as set in the HISCL settings. If there are not enough digits in the
date or time, the data is right-aligned with zero padding.
The format is the same as the format for this item in the “Query text”.
Send the same content as the query text.
6) 7.2.3.9 Rack No.

the rack number. If there are not enough digits, the data is right-aligned with zero padding.
The value is the same as for this item in the “Query text”.

Indicates the position of the sample in the sample rack.
Note:
8) 7.2.3.11 Sample ID
The sample ID is 13 ASCII characters. Spaces (2dh) and other symbols are included in the number of digits (13) of the sample
ID. If there are not enough digits, the number is right-aligned with space padding.
A sample ID in the format “QCXX” (where XX are any ASCII characters) is recognized by the HISCL as
a QC analysis order.
* When QC analysis is to be performed, the sample IDs must be made to match in the “QC material settings” of the HISCL.
Note:
Reserved for future use. All characters are set to spaces (20h).
10) 7.2.3.13 Parameter code

The alphanumeric characters of the host ID of each parameter to be analyzed are output right-aligned with zero padding.
A maximum of 24 analysis parameters, the maximum number that can be analyzed on the HISCL, are output. If the HISCL
receives an order for more than 24 analysis parameters, it performs only the first 24 tests in this field group and disregards the
remaining tests.
Note:
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The numeric characters of the dilution ratio are output right-aligned with space padding.
Dilutions that can be used are indicated below. If a different dilution or a dilution of 0 is received, the HISCL does not perform
analysis.
“1”, “40”, “1600”
Note:
The host must not send values other than 1, 40, and 1600 to the HISCL-800.
12) 7.2.3.15 Reserved

13) No analysis instruction

 No analysis instruction for the sample of a query
“000” is output for the parameter code in the analysis parameter instruction part of the instruction text format.
 No analysis instructions for samples following the sample of a query

“999” is output for the parameter code in the analysis parameter instruction part of the instruction text format.
Used when an abnormality is detected, such as no analysis instruction settings in the host computer or the sample ID or
sample tube position of the query is not in the analysis instruction.
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6.3.3. PAMIA-40i Compatible

(1) Sample ID subtext
7.2.4.4 Block number 2 “01” (fixed)
7.2.4.6 Sample distinction code 1 “U”, “E”, “S”, “C”
7.2.4.7 Year 4 YYYY
7.2.4.8 Month 2 MM
7.2.4.9 Day 2 DD
7.2.4.10 Time 4 HHMM
7.2.4.12 Sample tube position 2 Example: “06” Sample tube position “6”
Example: “Sample-01”, Sample ID
7.2.4.13 Sample ID 15
“Sample-01”
7.2.4.14 Sample ID attribute 1 “M”, “C”, “B”, “A”
7.2.4.15 Material 1 “1” Serum
(Reserved) 22 “”
7.2.4.27 Instrument ID 26 “HISCL-800^BF215796^11355”
Total 94
(2) Concentration subtext

7.2.4.4 Block number 2 “02”-“25”
7.2.4.16 Parameter code 3 Example: “001” Host ID “01”
7.2.4.17 Concentration 11 Example: “>123.45”, “ 999999.999”,
“---”
7.2.4.18 P/T(T1) 6 “0.00” (fixed)
7.2.4.19 P/T(T2) 6 “0.00” (fixed)
7.2.4.20 Analysis status (T1) 8 “” (fixed)
7.2.4.21 Analysis status (T2) 8 “OEX”
7.2.4.22 Negative/Positive 2 “”, “-”, “+-”, “+”, “1+”, “2+”, “3+”
7.2.4.23 Dilution ratio 5 Example: “40”, analysis with 1/40 dilution
7.2.4.24 Repeat analysis dilution ratio 5 Example: “1600”, repeat analysis with 1/1600 dilution
7.2.4.25 Repeat analysis 1 “0” (initial test), “1” (repeat test)
7.2.4.26 Reaction time 1 “2” (fixed)
7.2.4.27 Instrument ID 26 “HISCL-800^BF215796^11355”
Total 90
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This format consists of a combination of sample ID subtexts that hold sample control information such as the sample ID, and
concentration subtexts that hold the concentration of each analysis parameter.
Subtext combinations are as follows.
 Result of 1st parameter analysis: Sample ID subtext - Concentration subtext (end)

 Result of 2nd parameter analysis: Sample ID subtext - Concentration subtext of parameter 1 - Concentration subtext of
parameter 2 (end)
Order of output
The order of output is from the top parameter down, from the most significant digit of the data to the least significant digit.

Fixed characters are sent that indicate that the format is the analysis data text format of the PAMIA-40i format.

The total number of blocks of the sample ID subtext and concentration subtext, and the position within the total number. If there
are not enough digits in the date or time, the data is right-aligned with zero padding.

U Routine sample
S Sample for standard curve analysis
4) 7.2.4.7, 7.2.4.8, 7.2.4.9, 7.2.4.10 Date and time

The date and time of analysis is output. If there are not enough digits in the date or time, the data is right-aligned with zero
padding.
5) 7.2.4.11 Rack No.

the rack number. If there are not enough digits, the data is right-aligned with zero padding. When the STAT holder is used,
“000000” is output.
If alphabetical characters or symbols are used in the rack number on the HISCL, those characters or symbols are output.
Note:
The rack number values vary by model. Refer to Chapter 7.

Note:
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7) 7.2.4.13 Sample ID
Note:
8) 7.2.4.14 Sample ID attribute

An alphabetical character is output that indicates how the sample ID was determined.
M Manual entry
C Instruction from host computer
B Sample tube barcode
W (Not used)
A Automatically assigned by the HISCL
9) 7.2.4.15 Material
The sample type is output (whole blood, serum, urine).
The fixed number “1” is output to indicate serum.
Note:
On the HISCL-800 only serum is analyzed and no values other than “1” are transmitted. For details, refer to Chapter 7.
10) 7.2.4.16 Parameter No.

The alphanumeric characters of the host ID set on the HISCL for the analyzed parameter are output.
Note:
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11) 7.2.4.17 Concentration (index value)
The concentration is sent with a decimal point in the format displayed on the HISCL. Output in exponent format is not possible.
If the concentration is masked on the HISCL due to an analysis error, over-range, or other problem, “---” is
output. In this event, characters corresponding to the “Analysis Status” described below are output. Check the analysis status
before using the concentration that was output.
Parameter HISCL display Characters output

Mask Test error *****.*** “---”
Analysis error -----.--- “---”
Over range /
+++++.+++ “---”
Over range >123.456 “>123.456”
No standard curve XXXXX.XXX “---”
Flag Low reliability* 123.456* “123.456”
Over upper display limit >123.456 “>123.456”
Under lower display limit <123.456 “<123.456”
 A low reliability flag is not output in this field. Check the value of the “Analysis Status” described below before deciding
whether the value in this field can be used.
the upper analysis limit, “---” is output and “O” is output in the analysis status (T2).
If “Add ‘>’ to over-range results” is specified and the measured value is over the upper analysis limit, “>” and the upper
analysis limit value are output in this field, and “O” is output in the analysis status (T2).
Note:

(1) ++++
(2) > 2500
Note:
The concentration values vary by model. Refer to Chapter 7.
12) 7.2.4.18, 7.2.4.19 P/T (T1, T2)

“0.00” is output for both T1 and T2, right-aligned with space padding.
Note:
Fixed at “0.00” because the value of this field does not exist on the HISCL-800. For details, refer to Chapter 7.
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13) 7.2.4.20, 7.2.4.21 Analysis status (T1, T2)
Characters are output to indicate the status, such as an analysis error.
<T1>
“” is always output.
Note:
On the HISCL-800, this field is not used, and is always fixed at “**********” to prevent use. For details, refer to
Chapter 7.
<T2>
When there is no error, “” is output.
When there is an error, characters other than “” are output in the corresponding positions in the format “OEX”.
The information displayed on the HISCL corresponds as shown below to the characters that are output.
Characters
output
Error Over upper limit of analysis range (over range) O
Mask : Over range
Analysis error E
Mask : Test error, analysis error
Flag : Low reliability
No standard curve X
Mask : No standard curve
The fixed characters “” are output in the lowest two digits.
Note:
On the HISCL-800, only “O”, “E”, and “X” are transmitted. For details, refer to Chapter 7.
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14) 7.2.4.22 Negative/Positive
Characters are output that indicate the qualitative evaluation result. The information displayed on the HISCL corresponds as
shown below to the characters that are output.
Characters
output
Qualitative No evaluation “”
evaluation Negative (-) “-”
Pending (+-) “+-”
Positive (+) “+”
Low positive (1+) “1+”
Medium positive (2+) “2+”
High positive (3+) “3+”
Grouping parameter : Unable to evaluate (ND) “ND”
Grouping parameter : Pending (IND) “IN”
Grouping parameter : 2nd dilution analysis recommended (RE) “RE”
Note:
The HISCL-800 has more qualitative judgment items than the PAMIA-40i. For details, refer to Chapter 7.

The sample dilution ratio used for analysis is output. One of the following is output, right-aligned with space padding.
“1”, “40”, “1600”
Note:
On the HISCL-800, values other than 1, 40, and 1600 are not transmitted. For details, refer to Chapter 7.
16) 7.2.4.24 Repeat analysis dilution ratio

Outputs the retest dilution ratio when a retest order is created on the HISCL. The content output is the same as for the dilution
ratio above.
If a retest order has not been created, “0” is output, right-aligned with space padding.
17) 7.2.4.25 Repeat analysis

A numeric character is output to indicate initial test or retest.
“0” is output for initial test. “1” is output for retest.
18) 7.2.4.26 Reaction time

Fixed at “2”.
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19) 7.2.4.27 Instrument ID
The instrument ID is output.
The format is Instrument nameÎnstrument PS codeÎnstrument serial number.
The value is left-aligned with space padding.
Note:
Verify that the host can accept instrument names, instrument codes, and serial codes. For details, refer to Chapter 7.
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7.2.5.1 Text distinction code I 1 “R” (fixed)
7.2.5.2 Text distinction code II 1 “R” or “I”
7.2.5.7 Year 4 YYYY
7.2.5.8 Month 2 MM
7.2.5.9 Day 2 DD
Example: “”,
7.2.5.13 Sample ID 15
“Sample-01”
7.2.5.14 Reserved 2 “”
7.2.5.15 Type code 3 Example: “000”, “001”, “999”
7.2.5.16 Number of parameters 2 Example: “01”, “12”

Parameter code N Example: “099” Host ID “99”
Total 49+3×N
This format can be used to send the sample position, sample ID, and parameters that can be analyzed on the HISCL to the host
computer. A query for one sample is sent from the HISCL to the host computer using one query packet, and an analysis instruction is
sent from the host computer to the HISCL using one instruction packet.
Note:
If the number of items increases, the overall message length of the specified format will increase. Verify that the host can
handle the increased length. For details, refer to Chapter 7.
1) Order of output

Fixed characters are sent that indicate that the format is the query text format of the PAMIA-40i format.
Query whereby the rack number and sample tube position are used as keywords
R
and the sample ID is left blank.
I (alphabetical character “I”) Query whereby the sample ID is used as a keyword rather than being left blank.

A query consists of one text block, so these are always “01”.
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Always spaces (20H).
5) 7.2.5.7, 7.2.5.8, 7.2.5.9, 7.2.5.10 Date and time

The date and time of the query is output. If there are not enough digits in the date or time, the data is right-aligned with zero
padding.
6) 7.2.5.11 Rack No.

the rack number.
When the STAT holder is used, “000000” is output.
When the order is from the worklist, “000000” is output.
If alphabetical characters, symbols, or spaces are used in the rack number on the HISCL, those characters/symbols/spaces (20h)
are output.
Even if the query uses a sample ID as a keyword as described above, the corresponding rack number is output.
Note:
The rack ID values vary by model. Refer to Chapter 7.

“01” to “06” for a rack, or fixed at “01” for a sample in the STAT holder. When the order is from the worklist, the fixed number
“01” is output.
Even if the query uses a sample ID as a keyword as described above, the corresponding sample tube position is output.
Note:
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8) 7.2.5.13 Sample ID
If alphabetical characters or symbols are used in the sample ID on the HISCL, those characters or symbols are output. If a query
is made without specifying a sample ID, spaces “” are output (15 characters (20h)).
Even if the query uses a rack number and sample tube position as keywords as described above, the sample ID corresponding to
the rack number and sample tube position is output.

Section: IPU

When IsErrSmpIDHostSend is 0: Real-time inquiry for sample ID “ERRxxxxx” is not made.
When IsErrSmpIDHostSend is 1: Real-time inquiry for sample ID “ERRxxxxx” is made.
Note:
Reserved for future use. Currently all are set to spaces (20h). Do not perform a data check on this data.
10) 7.2.5.15 Type code

The desired order type that is sent to the host computer is output.
In a batch query, the number specified in the query is output, right-aligned with zero padding.
In a real-time query, “000” is output.
Decide the number that indicates the order type so that it corresponds with the host computer.

The number of parameters that can be analyzed based on the current HISCL settings is output, right-aligned with zero padding.
The number of parameters that can be analyzed is as set in the HISCL settings (parameter usage settings), with the maximum
number being “50”
Note:
Verify that the host can accept a number of parameters from 0 to 50. For details, refer to Chapter 7.
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12) 7.2.5.17 Parameter code 1 to N
The host ID of each analysis parameter that can be analyzed on the HISCL is output as right-aligned alphanumeric characters
with zero padding.
The host IDs are determined by the HISCL settings. A maximum of 50 analysis parameters are output as set in the HISCL
settings (parameter usage settings).
Note:
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7.2.6.1 Text distinction code I 1 “S” (fixed)
7.2.6.2 Text distinction code II 1 “0” (fixed)
7.2.6.7 Year 4 YYYY
7.2.6.8 Month 2 MM
7.2.6.9 Day 2 DD
7.2.6.13 Sample ID 15 Example: “Sample-01”
7.2.6.14 Material 1 “” or “1” (serum)
7.2.6.15 (Reserved) 11 “”
7.2.6.17 Dilution ratio (parameter 1) 5 “1”, analysis with x1 dilution
7.2.6.18 Reserved 2 “”

Dilution ratio (parameter N) 5 “ 1600”, analysis with 1/1600 dilution
Reserved 2 “”
Total 52+10×N
The host computer uses this format to specify analysis parameters and dilution ratios to the HISCL in response to a query for a sample.
The count for multiplex analysis is fixed at 1 in this instruction. In order to perform duplex analysis, this must be specified in the
HISCL settings in advance (host supplemental rack setting).
A query is made for one sample using one query packet, and an analysis instruction is returned using one instruction packet.
Note:
increased length. For details, refer to Chapter 7.
1) Order of output

Fixed characters are sent that indicate that the format is the instruction text format of the PAMIA-40i format.
“0” in text distinction code II is the number zero.

The instruction text consists of one text block, and thus both are always “01”.
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Always spaces (20H).
5) 7.2.6.7, 7.2.6.8, 7.2.6.9, 7.2.6.10 Date and time

The date and time of the query is output. If there are not enough digits in the date or time, the data is right-aligned with zero
padding.
The format is the same as the format for this item in the “Query text”. Send the same content as the query text.
6) 7.2.6.11 Rack ID
Sample rack ID consisting of 6 ASCII characters. Spaces (2DH) and other symbols are included in the number of digits of the
rack number.
Note:
The rack ID values vary by model. Refer to Chapter 7.

The format and values used are the same as for this item in the “Query text”.
Note:
8) 7.2.6.13 Sample ID
A sample ID in the format “QCXX” (where XX are any ASCII characters) is recognized by the
HISCL as a QC analysis order.
 When QC analysis is to be performed, the sample IDs must be made to match in the “QC material settings” of the HISCL.
Note:
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9) 7.2.6.14 Material
The sample type is output (serum, whole blood, urine) as a number.
“1” indicating serum or spaces (20h) are output.
Note:
On the HISCL-800 only serum is analyzed and no values other than “1” are transmitted. For details, refer to Chapter 7.
10) 7.2.6.15 Reserved

Reserved for future use.
All characters are set to spaces (20h).

The alphanumeric characters of the host ID of each parameter to be analyzed are output right-aligned with zero padding.
receives an order for more than 24 test parameters, it performs only the first 24 tests in this field group and disregards the
remaining tests.
Note:

The numeric characters of the dilution ratio are output right-aligned with space padding.
Dilutions that can be used are indicated below. If a different dilution or a dilution of “0” is received, the HISCL does not
perform analysis.
“1”, “40”, “1600”
Note:
Values other than 1, 40, and 1600 must not be sent. For details, refer to Chapter 7.
13) 7.2.6.18 Reserved

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6.3.4. ELSIA-FS1200 Special Mode Compatible

7.3.1.1 Text distinction code 1 “D” (fixed)
7.3.1.2 Instrument No. 1 “1” ( fixed)
7.3.1.3 Sample distinction code 1 “U”, “E”, “S”, “C”, “P”, “N”,
7.3.1.4 Sequence No. 4 “1” -“4999”
7.3.1.5 Sample ID 13 Example: “Sample-01”, sample ID “Sample-01”
7.3.1.6 Rack No. 4 Example: “RA-1” Rack No. “A-1”
7.3.1.7 Cup No. 1 Example: “6” Sample tube position “6”
7.3.1.8 Number of analysis parameters 2 “1”-“24”
7.3.1.9 Parameter code 1 2 Example: “1” Host ID “1”
7.3.1.10 Count value 1 5 Example: “1234” Count value 1,234
7.3.1.11 Concentration 1 6 Example: “1.23”, “123.4<”, “999999",
“”
7.3.1.12 Evaluation 1 3 “”, “H”, “L”, “+/-”, “+”
7.3.1.13 Remark 1 2 “” (fixed)

Count value N 5 Example: “99999” Count value 1,234,000
Concentration value N 6 Example: “1.23”, “>123.4”, “999999”,
“”
Evaluation N 3 “”, “H”, “L”, “+/-”, “+”
Remark N 2 “” (fixed)
Total 27+18×N
This format is used to send the sample ID and other sample control information, and the concentration of each analysis parameter, as a
single text block.
1) Order of output
2) 7.3.1.1 Text distinction

Fixed characters are sent that indicate that the format is the analysis results data text format of ELSIA-FS1200 special mode.
3) 7.3.1.2 Instrument No.

The number of the HISCL instrument that performed analysis is output as a numeric character.
The value is fixed at “1”.

U Routine sample
S Standard curve analysis sample (quantitative parameters C0 to C5)
P Positive control: Sample for standard curve analysis (qualitative parameter C1)
N Negative control: Sample for standard curve analysis (qualitative parameter C0)
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5) 7.3.1.4 Sequence No.
The analysis result transmission sequence number is output as a numeric character. If there are not enough digits, the number is
right-aligned with space padding. There is a separate range of sequence numbers for each sample type, and the number is
incremented after each output. When the range is exceeded, the number returns to the minimum value.
“1” to “2000” : Routine sample

“2001” to “3000” : Urgent sample (applies to all sample types analyzed in the STAT holder)
“3001” to “4000” : QC analysis
“4001” to “5000” : Standard curve analysis (quantitative parameters, qualitative parameters)
“Reception numbers” cannot be used on the HISCL.
6) 7.3.1.5 Sample ID
The sample ID is 13 ASCII characters. Spaces (2dh) and other symbols are included in the number of digits of the sample ID. If
there are not enough digits, the number is left-aligned with space padding.
Even if the query uses the sample position (rack number and cup number), the sample ID corresponding to the rack number and
sample tube position is output.
7) 7.3.1.6 Rack No.

Sample rack number consisting of 4 ASCII characters. “R” is output in the most significant digit to indicate “rack”, and the
remaining 3 digits are the number of the rack used for analysis. If the rack number does not have enough digits, the number is
right-aligned with zero padding. When the STAT holder is used, “” (4 characters (20h)) is output.
If the rack number is longer than 3 digits, the most significant digits are disregarded and the lower 3 digits are output.
Even if the sample of the query has a sample ID, the rack number corresponding to the sample is output.
8) 7.3.1.7 Cup No.

Indicates the position of the sample in the sample rack. “1” to “6” is output for a rack, or spaces (20h) for a sample in the STAT
holder.
Even if the sample of the query has a sample ID, the corresponding sample tube position in the rack is output.

Numeric characters indicating the number of analysis parameters are output. If there are not enough digits, the number is
right-aligned with space padding.

The alphanumeric host ID of the test parameter set on the HISCL is output.
If there are not enough digits, the number is left-aligned with space padding.
If right alignment / left alignment of this field is different on the host computer, avoid the discrepancy by making the number
two digits; for example, by filling in a zero in the HISCL settings so that the host ID is “01” instead of “1”.
Note:
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11) 7.3.1.10 Count value
The analysis result count value is output as a numerical value. If there are not enough digits, the number is right-aligned with
space padding.
If the count value is 100,000 or over, “99999” is output.
If the count value cannot be acquired due to an analysis error or other reason, “0” is output.
12) 7.3.1.11 Concentration

The concentration is sent with a decimal point in the format displayed on the HISCL. If there are too many digits, digits are
discarded from the least significant digit. If the concentration is 1 million or over, “999999” is output.
If the least significant digit is a decimal point, the decimal point is deleted and the number is right-aligned with space padding.
If the concentration is masked on the HISCL due to an analysis error, no standard curve, or other problem, “”
(20h) is output. In this event, “80” is output in “Remarks” described below. Check the concentration and remarks on the host
before deciding whether the “evaluation” that was output can be used.

Mask Test error . “”
Analysis error -----.--- “”
Over range / +++++.+++ “”
Over range >123.456 “123.4< ”
>9999.9 “ 9999< ”
>100000.00 “10000< ”
No standard curve XXXXX.XXX “”
Flag Low reliability 123.456 “123.45 ”
Over upper display limit >123.456 “123.4< ”
>9999.9 “ 9999< ”
>100000.00 “10000< ”
Under lower display limit <123.456 “123.4> ”
<9999.9 “ 9999> ”
<100000.00 “10000> ”
 When an over display limit or under display limit flag is sent, a maximum of 5 characters is sent for the concentration.
As such, for a high value where the integer part exceeds 6 digits, part of the transmitted content may be cut off. To avoid this
problem when using this flag, set the upper or lower display limit in the HISCL settings to “99999” or other value with an
integer part that is 5 digits or less.
 A low reliability flag is not output in this field. Check the value of “Remarks” described below before deciding whether the
value in this field can be used.
the upper analysis limit, “” (20h) is output and “80” is output in the remarks.
If “Add ‘>’ to over-range results” is specified, the analysis upper limit value and “<” are output in this field and “80” is
output in the “Remarks” when an analysis value is over the analysis upper limit.
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Note:

(1) ++++
(2) > 2500
13) 7.3.1.12 Evaluation

Characters are output that indicate the qualitative evaluation result and inside/outside concentration display range. The output
characters correspond to the evaluation results as shown below. Before using the value in this field, check the “Concentration”
above.
Characters
output
No qualitative Within display range 123.456 “”
evaluation
Over upper limit of display range > 123.456 “H”
Under lower limit of display range < 123.456 “L”
With qualitative Negative (-) “”
evaluation
Pending (+-) “+/-”
Positive (+) “+”
Low positive (1+) “+”
Medium positive (2+) “+”
High positive (3+) “+”
Grouping parameter Group 1 “1”
evaluation
Group 2 “2”
Unable to evaluate “ND”
Pending “IND”
Dilution repeat analysis recommended “RE”
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14) 7.3.1.13 Remarks
Remarks on the analysis results (error occurred / did not occur during analysis, etc.) are output as characters.
The information displayed on the HISCL corresponds as shown below to the characters output to the host.

Mask Test error . “80”
Analysis error -----.--- “80”
Standard curve analysis result -----.--- “80”
Over range /
+++++.+++ “80”
>123.456
Over range >9999.9 “80”
>100000.00
No standard curve XXXXX.XXX “80”
Flag Low reliability 123.456* “80”
>123.456
Over upper display limit >9999.9 “”
>100000.00
<123.456
Under lower display limit <9999.9 “”
<100000.00
For details on over range, refer to the “Concentration” section.
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7.3.2.1 Text distinction code 1 “R” (fixed)
7.3.2.2 Instrument No. 1 “1” (fixed)
 Sample ID included
Example: “Sample-01”, Sample ID “Sample-01”
7.3.2.3 Sample information 13  Sample ID is not decided

Example: “R0A15”
 (space padding),
Rack No. “A1”, Cup No. “5”
Total 15
A query for one sample is sent from the HISCL to the host computer using one query packet, and an analysis instruction is sent from
the host computer to the HISCL using one instruction packet.
Sample information is output with Rack No./Cup No. or sample ID.
1) Order of output

Fixed characters are sent that indicate that the format is the analysis results query text format of ELSIA FS-1200 special mode.

The number of the HISCL instrument that made the analysis order query is output as a numerical value.
4) 7.3.2.3 Sample information (Sample ID)

The sample ID is 13 ASCII characters. If there are not enough digits, the number is left-aligned with space padding.
If the sample ID of the sample of the query is not decided, the query is made using the (5) Rack No. and (6) Cup No.

Section: IPU

When IsErrSmpIDHostSend is 0: Real-time inquiry for sample ID “ERRxxxxx” is not made.
When IsErrSmpIDHostSend is 1: Real-time inquiry for sample ID “ERRxxxxx” is made.
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5) 7.3.2.3 Sample information (Rack No.)
right-aligned with zero padding. When the STAT holder is used, “” (20h) is output. When the order is from the
worklist, “” (20h) is output.
If the rack number is longer than 3 digits, the most significant digits are disregarded and the lower 3 digits are output.
6) 7.3.2.3 Sample information (Cup No.)

Indicates the position of the tube in the sample rack. “1” to “6” is output for a rack, or spaces (20h) for a sample in the STAT
holder. When the order is from the worklist, spaces (20h) are output.
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7.3.3.1 Text distinction code 1 “S” (fixed)
7.3.3.2 Instrument No. 1 “1” (fixed)
7.3.3.3 Sample distinction code 1 “U” (fixed)
7.3.3.4 Reception No. 4 “”(fixed)
7.3.3.5 Sample ID 13 “Sample-01”, Sample ID “Sample-01”
7.3.3.6 Rack No. 4 “R0A1” Rack ID “A1”
7.3.3.7 Cup No. 1 “6” Cup No. “6”
7.3.3.8 Number of analysis parameters 2 “0”-“24”
7.3.3.9 Analysis parameter code 1 2 Example: “1 ” Host ID “1”

Analysis parameter code N 2 Example: “99” Host ID “99”
Total 27+2×N
The host computer uses this format to specify analysis parameters to the HISCL in response to a query for a sample. The dilution ratio
and multiplex analysis count cannot be specified in this format. These must be specified in the HISCL settings in advance (host
supplemental rack setting).
A query is made for one sample using one query format, and an analysis instruction is returned using one instruction format.
Order of output

Fixed characters are sent that indicate that the format is the instruction text format of ELSIA-FS1200 special mode.

The number of the HISCL instrument that made the analysis order query is output as a numerical value.

Standard curve distinction cannot be used on the HISCL (this is treated as a normal sample).
4) 7.3.3.4 Reception No.

Output “” (20h) fixed.
This data is not used on the HISCL.
5) 7.3.3.5 Sample ID
The sample ID is 13 ASCII characters. If there are not enough digits, the number is left-aligned with space padding.
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6) 7.3.3.6 Rack No.
right-aligned with zero padding.
7) 7.3.3.7 Cup No.

The format and values used are the same as for this item in the “Query text”.

The number of analysis parameters specified is output as a numerical value. If there are not enough digits, the number is
right-aligned with space padding. On the HISCL, the maximum of number of analyzable parameters is “24”. Do not output
more than 24 parameters. If the HISCL receives a number greater than “24”, it performs only the first 24 tests of the “Analysis
parameter codes” and disregards the remaining tests.
9) 7.3.3.9 Analysis parameter code

The alphanumeric characters of the host ID of each parameter to be analyzed are output, left-aligned with space padding.
receives an order for more than 24 analysis parameters, it performs only the first 24 tests in this field group and disregards the
remaining tests.
If right alignment / left alignment of this field is different on the host computer, avoid the discrepancy by making the number
two digits; for example, by filling in a zero in the HISCL settings so that the host ID is “01” instead of “1”.
Note:
10) No analysis instruction

If there is no analysis instruction for the sample of a query, “0” is output for the number of analysis parameters.
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6.3.5. ELSIA-FS1200 Exclusive Mode Compatible

Text distinction code 1 “D” (fixed)
Instrument No. 1 “1” (fixed)
Sample distinction code 1 “U”, “E”, “S”, “C”, “P”, “N”
Sequence No. 4 “1”-“4999”
Sample ID 13 Example: “Sample-01”, sample ID “Sample-01”
7.4.1.1 Date tested 8 YYYYMMDD
7.4.1.2 Time tested 6 HHMMSS
Rack No. 4 Example: “RA-1” Rack ID. “A-1”
Cup No. 1 Example: “6” Sample tube position “6”
Number of analysis parameters 2 “1”-“24”
Parameter code 1 2 Example: “1” Host ID “1”
Count value 1 5 Example: “1234” Count value 1,234
Concentration value 1 6 Example: “1.23”, “>123.4”, “999999”, “”
Evaluation 1 3 “”, “H”, “L”, “+/-”, “+”
Remarks 1 2 “” (fixed)

Count value N 5 Example: “99999” Count value 1,234,000
Concentration value N 6 Example: “1.23”, “123.4>”, “ 999999”, “”
Evaluation N 3 “”, “H”, “L”, “+/-”, “+”
Remark N 2 “” (fixed)
Total 41+18×N Variable
This format is used to send the sample ID and other sample control information, and the concentration of each analysis parameter, as a
single text block.
This format adds “Analysis Date and time” to ELSIA-FS1200 special mode. All data other than “Analysis date and time” is the same
as special mode.
1) 7.4.1.1, 7.4.1.2 Analysis date and time

Outputs the date and time of analysis. If there are not enough digits, the data is right-aligned with zero padding.
The format is fixed at YYYYMMDD and does not depend on the HISCL settings.

The query format is the same as “ELSIA-FS1200 special mode”.

The host computer uses this format to specify analysis parameters to the HISCL in response to a query for a sample. The dilution ratio
and multiplex analysis count cannot be specified in this format. These must be specified in the HISCL settings in advance (host
supplemental rack setting).
The instruction format is the same as “ELSIA-FS1200 special mode”.
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7. Special Remarks
7.1. Order Query Checking

An order instruction response is received from the host in response to an order query from the HISCL, and at this time, the sample ID
is checked. There are two keywords for sample checking, the “Sample ID” and “Rack No. and sample position”.
For “Sample ID”, when the Sample ID of the order request does not match the Sample ID of the instruction, either “Do not analyze”
or “Give priority to instruction and analyze” can be specified in the HISCL settings to operate accordingly.
If the “Rack No. and sample position” do not match in the query and the instruction, analysis is not performed.
7.2. Order Query Method

 A real-time query is made immediately before analysis using the sample ID read from the barcode label on the sample tube.
 However, if the analysis order is already registered in the I110M, a query is not made and the registered order is used.
 A batch query specifying rack numbers or sample IDs is made before analysis.
 For batch queries made with the ELSIA-FS1200 (special mode, exclusive mode), the “ELSIA batch format” cannot be used; only
the “ELSIA real-time formats” (the query and instruction formats described in this document) can be used.
7.3. Cautionary Points Regarding Auto Output of Analysis Results

When the HISCL is set to automatically send analysis results, there is a possibility that transmission of batch queries / real-time order
queries will alternate with transmission of analysis results. If it is likely that the host computer response will be delayed due to this
situation, send analysis results by batch transmission after analysis is finished rather than by auto transmission.
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7.4. Messages that change due to the HISCL settings
The messages indicated in the table below change due to the HISCL-800 settings.
Parameter Communication format Record Description
Parameter ID ASTM Analysis result format For the parameter IDs, a number is set for each
PAMIA-50 Query format parameter based on the host ID setting of each
PAMIA-40i Instruction format HISCL-800 parameter.
ELSIA-FS1200 Special
ELSIA-FS1200 Exclusive
Concentration ASTM Analysis result format The transmitted content of the ASTM result record and
PAMIA-50 the various PAMIA/ELSIA analysis result formats
PAMIA-40i varies depending on the display setting for over-range
ELSIA-FS1200 Special results on the HISCL-800. (Refer to 7.4.1)
Concentration ASTM Analysis result format The number of decimal digits on the HISCL-800 varies
PAMIA-50 depending on the setting of each parameter.
PAMIA-40i
Units ASTM Analysis result format The representation of the units sent to the host varies
depending on the setting for each parameter on the
HISCL-800.
Instrument ID ASTM Header record (ASTM) The HISCL-800 instrument code and instrument serial
PAMIA-40i Analysis result format number vary by instrument.
(PAMIA-40i) *The instrument code differs between Japan and other
countries/regions, and the instrument serial number
varies by instrument case.
7.4.1. Analysis result format message for over-range results

The transmitted content of the LIS2/ASTM result record and the various PAMIA/ELSIA analysis result formats varies depending on the
display setting for over-range results on the HISCL.
Example: Analysis parameter HBsAg (upper limit value of 2500)
On the HISCL, the analysis result screen display and output to host can be selected from the following 3 types:
(1) ++++
(2) > 2500
(3) >100000, >4000000 (displays upper limit value × dilution ratio)
The content output to the host depends on this setting as shown below.
■LIS2/ASTM
HISCL setting Message content
++++ R|1|^^^01^HBsAg_F^1|++++|IU/mL||A||||||20150325100234|[CR]
>2500 R|1|^^^01^HBsAg_F^1|2500.000|IU/mL||A||||||20150325100639|[CR]
>100000 (x40 dilution) R|1|^^^01^HBsAg_F^40|100000.000|IU/mL||A||||||20150325100653|[CR]
>4000000 (x1600 dilution) R|1|^^^01^HBsAg_F^1600|4000000.00|IU/mL||A||||||20150325100704|[CR]
*With the (1) ++++ setting, only the dilution ratio changes if a x40 dilution analysis result is over-range.
R|1|^^^01^HBsAg_F^40|++++|IU/mL||A||||||20150318164021|[CR]
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■PAMIA-50
++++ DS310202001 --- 0.00 0.00********O*****+* 1 002
>2500 DS310202001 >2500.000 0.00 0.00********O*****+* 1 002
>100000 (x40 dilution) DS310202001>100000.000 0.00 0.00********O*****+* 40 002
>4000000 (x1600 dilution) DS310202001>4000000.00 0.00 0.00********O*****+* 1600 002
DS310202001 --- 0.00 0.00********O******* 40 002
■PAMIA-40i
++++ DS330202001 --- 0.00 0.00********O*******+ 1 002HISCL-800^0^00000
>2500 DS330202001 >2500.000 0.00 0.00********O*******+ 1 002HISCL-800^0^00000
>100000 (x40 dilution) DS330202001>100000.000 0.00 0.00********O*******+ 40 002HISCL-800^0^00000
>4000000 (x1600 dilution) DS330202001>4000000.00 0.00 0.00********O*******+ 1600 002HISCL-800^0^00000
DS330202001 --- 0.00 0.00********O******* 40 002HISCL-800^0^00000
■ELSIA-FS1200 Special Mode

++++ D1U 13 R0031 10199999 + 80
>2500 D1U 24 R0041 101999992500.<+ 80
>100000 (x40 dilution) D1U 35 R0042 1019999910000<+ 80
>4000000 (x1600 dilution) D1U 46 R0043 1019999999999<+ 80
■ELSIA-FS1200 Exclusive Mode

++++ D1U 13 20150325100234R0031 10199999 + 80
>2500 D1U 24 20150325100639R0041 101999992500.<+ 80
>100000 (x40 dilution) D1U 35 20150325100653R0042 1019999910000<+ 80
>4000000 (x1600 dilution) D1U 46 20150325100704R0043 1019999999999<+ 80
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7.5. Differences between HISCL-800 LIS2 format and HISCL-800 ASTM format
The differences between HISCL-800 LIS2 format and HISCL-800 ASTM format are summarized in the table below.
■Table of differences between HISCL-800 LIS2 format and ASTM format

Parameter HISCL-800 HISCL-800 Cautionary notes
LIS2 format ASTM format
Version information LIS2-A2 E1394-97 The message sent in the
(Header record) version information varies
depending on the selected
communication format.
Sample ID Maximum 22 digits Maximum 15 digits The maximum number of
(Query record) digits of the sample ID
(Analysis Order record) depends on the communication
specifications. Consider the
number of sample ID digits
used in your facility when
selecting a communication
format.
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7.6. Differences in the message between the HISCL-800 and other models
The differences in the communication formats of the HISCL-800 and the HISCL-2000i, HISCL-5000, PAMIA-50, and PAMIA-40i are
summarized in the tables below.
■HISCL-800 and HISCL-5000 comparison table

HISCL-5000
LIS2 ASTM PAMIA-50 PAMIA-40i ELSIA ELSIA
Exclusive Special
LIS2
Refer to 7.6.1
HISCL-800
ASTM
PAMIA-50 Refer to 7.6.2
PAMIA-40i Refer to 7.6.3
ELSIA-FS1200 Exclusive None
ELSIA-FS1200 Special None
*Diagonal lines indicate that a difference is not applicable because the message format is different.
■HISCL-800 and HISCL-2000i comparison table

HISCL-2000i
ASTM PAMIA-50 PAMIA-40i ELSIA ELSIA
Exclusive Special
LIS2 Refer to 7.6.4
HISCL-800
ASTM Refer to 7.6.5

ELSIA-FS1200 Exclusive None
ELSIA-FS1200 Special None
■HICSL-800 and PAMIA-50, PAMIA-40i comparison table

PAMIA-50 PAMIA-40i
Communication Communication
specifications specifications
LIS2
HISCL-800
ASTM
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7.6.1. Differences between HISCL-800 LIS2 (ASTM) format and HISCL-5000 LIS2 (ASTM) format
The differences between HISCL-800 LIS2 format and HISCL-5000 LIS2 format are summarized in the table below.
The differences between HISCL-800 ASTM format and HISCL-5000 ASTM format are summarized in the table below.
Parameter HISCL-800 HISCL-5000 Cautionary notes

Sender name or ID Instrument name: HISCL-800 Instrument name: The host must be able to receive
(Header record) HISCL-5000 when the instrument name is
HISCL-800.
The host must be able to receive
the values of PS codes that differ
between Japan and other
counties/regions, and serial
numbers that vary by instrument.
*For differences between LIS2 format and ASTM format that do not depend on the model, refer to 7.5.
7.6.2. Differences in PAMIA-50 format of the HISCL-800, HISCL-5000, and HISCL-2000i

The PAMIA-50 format of the HISCL-800 differs from the PAMIA-50 format of the HISCL-5000 and the PAMIA-50 format of the
HISCL-2000i as indicated below.
Parameter HISCL-800 HISCL-5000 HISCL-2000i Cautionary notes

PAMIA-50 format PAMIA-50 format PAMIA-50
format
Sample tube Fixed at “1” for urgent Fixed at “1” for urgent “1” to “6” for an The HISCL-5000 and
position sample sample urgent sample 800 only have 1 STAT
(Analysis result “1” to “6” for a routine “1” to “6” for a routine “1” to “10” for sample position,
format) sample sample routine sample therefore the sample
(Analysis order tube position
query format) information sent for an
(Instruction urgent sample is fixed
format) at “1”.
For a routine sample,
“1” to “6” is sent on the
HISCL-5000 and 800.
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7.6.3. Differences in the PAMIA-40i formats of the HISCL-800, HISCL-5000, and HISCL-2000i
The PAMIA-40i format of the HISCL-800 differs from the PAMIA-40i format of the HISCL-5000 and the PAMIA-40i format of the
HISCL-2000i as indicated below.
Parameter HISCL-800 HISCL-5000 HISCL-2000i Cautionary notes

PAMIA-40i PAMIA-40i PAMIA-40i
format format format
Instrument ID HISCL-800 is entered HISCL-5000 is entered HISCL-2000i is The host must be able to
(Analysis result in Instrument Name. in Instrument Name. entered in Instrument receive when the instrument
format) Name. name is HISCL-800.
The host must be able to
receive the values of PS codes
that differ between Japan and
other counties/regions, and
serial numbers that vary by
instrument.
Sample tube position Fixed at “1” for urgent Fixed at “1” for urgent “1” to “6” for an The HISCL-5000 and 800
(Analysis result sample sample urgent sample only have 1 STAT sample
format) “1” to “6” for a routine “1” to “6” for a routine “1” to “10” for position, therefore the sample
(Analysis order query sample sample routine sample tube position information sent
format) for an urgent sample is fixed
(Instruction format) at “1”.
For a routine sample, “1” to
“6” is sent on the
HISCL-5000 and 800.
7.6.4. Differences between HISCL-800 LIS2 format and HISCL-2000i ASTM format
The differences between HISCL-800 LIS2 format and HISCL-2000i ASTM format are indicated below.
Parameter HISCL-800 HISCL-2000i Cautionary notes

LIS2 format ASTM format
Sender name or ID HISCL-800 is entered in HISCL-2000i is entered The host must be able to receive when the
(Header record) Instrument Name. in Instrument Name. instrument name is HISCL-800.
The host must be able to receive the values of PS
codes that differ between Japan and other
counties/regions, and serial numbers that vary by
instrument.
Version information “LIS2-A2” “E1394-97” The message sent in the version information
(Header record) (Fixed) is entered. (Fixed) is entered. varies depending on the selected communication
format.
Sample tube position Fixed at “1” for an urgent “1” to “6” for an urgent The HISCL-800 only has 1 STAT sample
(Query record) sample. sample position, therefore the information sent for the
(Analysis Order “1” to “6” for a routine “1” to “10” for a routine sample tube position is fixed at “1”.
record) sample. sample. “1” to “6” for a routine sample.
(Result record)
Sample ID Maximum of 22 digits. Maximum of 15 digits. The maximum number of digits of the sample ID
(Query record, Alphabetical, numeric, Alphabetical, numeric, depends on the communication specifications.
analysis order record, and symbol characters. and symbol characters. Consider the number of sample ID digits used in
result record) your facility when selecting a communication
format.
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7.6.5. Differences between HISCL-800 ASTM format and HISCL-2000i ASTM format
The differences between HISCL-800 ASTM format and HISCL-2000i ASTM format are indicated below.
Parameter HISCL-800 HISCL-2000i Cautionary notes

ASTM format ASTM format
Sender name or ID HISCL-800 is entered in HISCL-2000i is entered The host must be able to receive when the
(Header record) Instrument Name. in Instrument Name. instrument name is HISCL-800.
The host must be able to receive the values of PS
codes that differ between Japan and other
counties/regions, and serial numbers that vary by
instrument.
Sample tube position Fixed at “1” for an urgent “1” to “6” for an urgent The HISCL-800 only has 1 STAT sample
(Query record) sample. sample position, therefore the information sent for the
(Analysis Order “1” to “6” for a routine “1” to “10” for a routine sample tube position is fixed at “1”.
record) sample. sample. “1” to “6” for a routine sample.
(Result record)
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7.6.6. Differences between PAMIA-50 format of the HISCL-800 and PAMIA-50 communication specifications
The differences between the PAMIA-50 format of the HISCL-800 and the PAMIA-50 host specifications are indicated below.
■ Analysis result format

Parameter HISCL-800 PAMIA-50 Cautionary notes
Sample tube Fixed at “1” for an urgent sample. “01” to “10” regardless of whether or The HISCL-800 only has 1
position “1” to “6” for a routine sample. not the sample is urgent. STAT sample position, therefore
the information sent for the
sample tube position is fixed at
“1”.
“1” to “6” for a routine sample.
Sample ID Alphabetical, numeric, and symbol Numeric characters only. Verify that the host can receive
characters. alphabetical, numeric, and
symbol characters for the sample
ID.
Parameter A parameter code based on the A parameter code based on the Check the HISCL-800 host ID
Code HISCL-800 settings is entered. PAMIA-50 settings is entered. setting and conform to that
setting.
Concentration Output in exponent format is not Output in exponent format with 6 Verify that the host can accept a
performed. digits for the mantissa and 2 digits for concentration that is not in
The number of decimal digits output the exponent. exponent format.
conforms to the setting for each
parameter on the HISCL-800.
Output example: Output example:

123.000 1.23000E+02
Concentration For analysis results without a For analysis results without a The HISCL-800 cannot
calibration curve, 3 right-aligned calibration curve, the same number of determine from the concentration
characters of “-” (2dH) are entered, spaces (20H) as the number of if a “no calibration curve” error
and spaces (20H) are entered in blank characters are entered. has occurred.
parts. If “no calibration curve” is to be
determined by the host, the
analysis state (T2) must be
referenced. (Refer to section
6.3.5.1)
P/T(T1) “ 0.00” (fixed) is entered. 3 digits are entered in the integer part, Fixed at “ 0.00” because the
2 digits are entered in the decimal value of this field does not exist
point and decimal digit part, and blank on the HISCL-800.
parts are filled with spaces (20H).
P/T(T2) “ 0.00” (fixed) is entered. 3 digits are entered in the integer part, Fixed at “ 0.00” because the
2 digits are entered in the decimal value of this field does not exist
point and decimal digit part, and blank on the HISCL-800.
parts are filled with spaces (20H).
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Analysis “********” (fixed) is entered. “********” when there is no error. On the HISCL-800, this field is
Status (T1) When there is an error, the characters not used, and is always fixed at
“*BEN****” are output in accordance “**********” to prevent use.
with the following.
O: Over range
B: Background error
E: Distribution error, count error,
blank value error
N: No sample, no reagent
D: Inappropriate dilution ratio. In this
case, O is also output.
X: Unable to calculate concentration
because there is no calibration curve
+ : Concentration exceeds the upper
limit of the reference interval
- : Concentration under the lower limit
of the reference interval
(When within the reference interval,
both + and - are output)
1+: Output as qualitative parameter.
Analysis When there is no error and a “********” when there is no error. The HISCL-800 has additional
Status (T2) qualitative evaluation is not made, When there is an error, the characters qualitative judgment items.
“********” is output. “0BENDX+-” are output in Ensure that the host can accept
When there is an error or a qualitative accordance with the following. these items.
evaluation is made, characters other O: Over range
than “*” are output in the B: Background error
corresponding positions in the format E: Distribution error, count error,
“O*E**X+-”. blank value error
N: No sample, no reagent
For O, E, X, +, -, refer to section D: Inappropriate dilution ratio. In this
6.3.2.1 of this document. case, O is also output.
X: Unable to calculate concentration
because there is no calibration curve
+ : Concentration exceeds the upper
limit of the reference interval
- : Concentration under the lower limit
of the reference interval
(When within the reference interval,
both + and - are output)
1+: Output as qualitative parameter
Dilution ratio 1, 40, or 1600. 1 or 5 to 1000. On the HISCL-800, values other
than 1, 40, and 1600 are not
transmitted.
107 R314168
■ Analysis order query format
Message The maximum message length is 194 The maximum message length is 71 If the number of items increases,
length bytes (44 + 3 × 50). bytes (44 + 3 × 9). the overall message length of the
specified format will increase.
Verify that the host can handle
the increased length.
Sample Fixed at “1” for an urgent sample. “01” to “10” regardless of whether or The HISCL-800 only has 1
tube “1” to “6” for a routine sample. not the sample is urgent STAT sample position, therefore
position the information sent for the
“1”.
ID.
setting.
■ Instruction format
Verify that the host can handle
the increased length.
Sample Fixed at “1” for an urgent sample. “01” to “10” regardless of whether or The HISCL-800 only has 1
tube “1” to “6” for a routine sample. not the sample is urgent. STAT sample position, therefore
position the information sent for the
“1”.
Sample ID Alphabetical, numeric, and symbol Numeric characters only. Verify that the host can send
ID.
setting.
Dilution Set 1, 40, or 1600. Set 1 or 5 to 1000. The host must not send values
Ratio other than 1, 40, and 1600 to the
HISCL-800.
108 R314168
7.6.7. Differences between PAMIA-40i format of the HISCL-800 and PAMIA-40i communication specifications
The differences between the PAMIA-40 format of the HISCL-800 and the PAMIA-40i host specifications are indicated below.
■ Analysis result format

Parameter HISCL-800 PAMIA-40i Cautionary notes
Sample tube Fixed at “1” for an urgent sample. “01” to “10” regardless of whether or The HISCL-800 only has 1
position “1” to “6” for a routine sample. not the sample is urgent. STAT sample position, therefore
the information sent for the
“1”.
ID.
Rack ID 6 alphabetical, numeric, and symbol 000000 to 000005 is set. Verify that the host can receive
characters are set. alphabetical, numeric, and
symbol characters for the rack
ID.
Material Serum is always fixed at “1”. “1” specifies serum, “2” specifies On the HISCL-800 only serum is
whole blood. analyzed and no values other
“3” specifies urine. (In mixed analysis than “1” are transmitted.
of B2M in serum and B2M in urine,
this is output for B2M in urine.)
Parameter ID The host ID set on the HISCL-800 is The transmission item number in the Check the HISCL-800 host ID
output. PAMIA settings is entered. setting and conform to that
setting.
Concentration Output in exponent format is not The data is sent in exponential format If your system has previously
performed. (1.2345E01, etc.) or display format used exponential format for the
The data is transmitted using the (“123.45” or “ >1000.00”) in concentration in PAMIA-40i,
number of decimal digits set for each accordance with the PAMIA setting. verify that the host can accept the
parameter of the HISCL-800. concentration in a format other
than exponential format.
P/T(T1) “ 0.00” (fixed) is entered. 3 digits in the integer part and 2 digits Fixed at “ 0.00” because the
in the decimal point and decimal digit value of this field does not exist
part. Blank parts are filled with spaces. on the HISCL-800.
P/T(T2) “ 0.00” (fixed) is entered. 3 digits in the integer part and 2 digits Fixed at “ 0.00” because the
in the decimal point and decimal digit value of this field does not exist
part. Blank parts are filled with spaces on the HISCL-800.
Analysis Status “********” (fixed) is entered. “********” when there is no error. On the HISCL-800, this field is
(T1) When there is an error, “*SEN****” is not used, and is always fixed at
sent in accordance with the following. “**********” to prevent use.
O: Over range
S: Sample aspiration failure
blank value error, background error
N: No sample
D: Inappropriate dilution ratio
M: Abnormal sample
109 R314168
Analysis Status When there is no error, “********” is “********” when there is no error. On the HISCL-800, only “O”,
(T2) output. When there is an error, “E”, and “X” are transmitted.
When there is an error, characters other “O*ENDX*M” is sent in accordance
than “*” are output to T2 in the with the following.
corresponding positions in the format O: Over range
“O*E**X**”. S: Sample aspiration failure
For O, E, X, refer to section 6.3.3.1 of blank value error, background error
this document. N: No sample
D: Inappropriate dilution ratio
M: Abnormal sample
Negative/Positive Judgment results are sent in the Judgment results are sent in the The HISCL-800 has additional
patterns below. For the meaning of patterns below. qualitative judgment items.
each pattern, refer to section 6.3.3.1 of + : Concentration is over the upper Ensure that the host can accept
this document. limit of the reference interval these items.
“ “ (20H) - : Concentration is under the lower
“ -” limit of the reference interval
“+-” +-: Within the reference interval
“+ “ 1+, 2+, 3+: When positive level is
“1+” judged and output
“2+”
“3+”
“*1”
“*2”
“ND”
“IN”
“RE”
Dilution Ratio 1, 40, or 1600 1 or 5 to 1000 On the HISCL-800, values other
than 1, 40, and 1600 are not
transmitted.
Instrument ID HISCL-800 instrument name, PAMIA-40i instrument name, Verify that the host can accept
instrument code, and instrument serial instrument code, and instrument serial instrument names, instrument
number are entered. number are entered. codes, and serial codes.
110 R314168
■ Analysis order query format
Verify that the host can handle the
increased length.
Rack ID 6 alphabetical, numeric, and symbol 000000 to 000005 is set. Verify that the host can receive
characters are set. alphabetical, numeric, and symbol
characters for the rack No.
Sample Fixed at “1” for an urgent sample. “01” to “10” regardless of whether or The HISCL-800 only has 1 STAT
tube “1” to “6” for a routine sample. not the sample is urgent. sample position, therefore the
position information sent for the sample
tube position is fixed at “1”.
characters. alphabetical, numeric, and symbol
characters for the sample ID.
Number of 01 to 50 are sent in accordance with the 01 to 07 are sent. Verify that the host can accept
Parameters HISCL-800 settings. values of 01 to 50.
Parameter The host ID number is sent in The transmission item number in the Check the HISCL-800 host ID
ID accordance with the HISCL-800 PAMIA settings is sent. setting and conform to that setting.
settings.
■ Instruction format
Verify that the host can handle the
increased length.
Rack ID 6 alphabetical, numeric, and symbol 000000 to 000005 is set. Verify that alphabetical, numeric,
characters are set. and symbol characters can be
entered and sent for the rack ID
Sample Fixed at “1” for an urgent sample. “01” to “10” regardless of whether or The HISCL-800 only has 1 STAT
tube “1” to “6” for a routine sample. not the sample is urgent. sample position, therefore the
position information sent for the sample
tube position is fixed at “1”.
Sample ID Alphabetical, numeric, and symbol Numeric characters only. Verify that the host can send
characters. alphabetical, numeric, and symbol
characters for the sample ID.
Parameter The host ID number is sent in The transmission item number in the Check the HISCL-800 host ID
Code accordance with the HISCL-800 PAMIA settings is sent. setting and conform to that setting.
settings.
Dilution 1, 40, or 1600. 1 or 5 to 1000. The host must not send values
Ratio other than 1, 40, and 1600 to the
HISCL-800.
[End of document]
111 R314168

MD - HISCL-800 - Host Specifications - v6.00

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HISCL-800

Host Interface Specifications

Automated Immunoassay Analyzer (Chemiluminescent Enzyme Immunoassay Analyzer) HISCL-800

Revision Date Change

1.00 April 10, 2013 Initial version

7.3.1 Analysis result format

7.4.1 Analysis result format

Global change: “IPU” to “HISCL”.

Corrected comment on the list in 5.2.4 Communication parameters to “The

In 7.2.4.27 Instrument ID in 7.3.1 Analysis result format, added description

Chapter number Description

 Rack number and sample ID:

 Numerical values such as the concentration:

[ex] When the concentration is “123456789.123”, “123456789.1” is set.

 If the data type is numerical value, the value “0” is used.

Communication specifications are based on a layer protocol.

(1) Physical layer

(2) Data link layer

(3) Presentation layer

Presentation layer ← Defines message specifications

Serial connection TCP/IP connection

Table 1: Connector pin assignments

5.1.1.2. Signal identification levels

Table 2: Signal identification levels

5.1.1.3. Connection cable

HISCL Host computer

5.1.2. Data Link Layer (Transmission Protocol)

5.1.2.1. Communication statuses

Transition to each status is accomplished through the following 3 phases.

(2) Transfer phase

(3) Termination phase

Neutral status Linked status Transfer phase

Max. 15 sec. Max. 15 sec. At least

5.1.2.3. Transfer phase

(2) Multiple records cannot be included in a single frame.

[STX] [F#] [Text] [ETX] [CHK1] [CHK2] [CR] [LF]

[STX] [F#] [Text] [ETB] [CHK1] [CHK2] [CR] [LF]

Example: [STX]3C|A |あｱ[CR] [ETX]

3 C | A (Space) ｜ あ ｱ [CR] [ETX]

[CR] [LF] Control code indicating end of frame.

Max. 15 sec. Max. 15 sec.

5.1.2.4. Termination phase

(1) Establishment phase

(2) Transfer phase

Patient information P 1 Patient information

Analysis results R 3 Analysis results

Comment record C 1-4 Sample comments or patient comments

Manufacturer information M N/A Not used

Scientific information S N/A Not used

Message termination L 0 Indicates the end of the message.

 A smaller level number indicates a higher level.

[Escape delimiters corresponding to function characters]

However, when multi-language is used for text such as names and

Figure 1: Images of Records, Fields, and Sub-fields

Message Record 1 Record 2  Record n

ID RS Field 1 RS Field 2 RS  RS Field n CR

Record identifier Delimiter ‘|’ or ‘\’

Sub-field 1 SS Sub-field 2 SS  SS Sub-field n

Figure 2: Omitting fields

Message Record 1 Record 2  Record n

No omission ID RS Field 1 RS RS  RS CR

Figure 3: Omitting sub-fields

Message Record 1 Record 2  Record n

3 C | A (Space) ｜あｱ [CR] [ETX]