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High Performance Thin Layer Chromatography

High Performance Thin Layer Chromatography (HPTLC) is an advanced and automated version of traditional thin layer chromatography, offering a fast and cost-effective separation method. It is widely used in various fields such as pharmaceuticals, food analysis, clinical applications, industrial processes, and forensic investigations. HPTLC provides advantages including rapid processing, accuracy, and precision compared to other separation techniques.

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Megha Kamble
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232 views4 pages

High Performance Thin Layer Chromatography

High Performance Thin Layer Chromatography (HPTLC) is an advanced and automated version of traditional thin layer chromatography, offering a fast and cost-effective separation method. It is widely used in various fields such as pharmaceuticals, food analysis, clinical applications, industrial processes, and forensic investigations. HPTLC provides advantages including rapid processing, accuracy, and precision compared to other separation techniques.

Uploaded by

Megha Kamble
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

High performance thin

layer chromatography
(HPTLC)
INTRODUCTION

• High performance thin layer chromatography is an improved, sophisticated


and an automated form of thin layer chromatography.
• It is also known as planar chromatography or Flat-bed chromatography
• HPTLC is a well known and versatile separation method which shows a lot
of advantages and options in comparison to other separation techniques.
• The method is fast and inexpensive. It does not require time consuming
pre-treatments.
• Automated, sophisticated form and improved method of TLC.
APPLICATIONS

• Pharmaceutical Industry: quality control, content uniformity,


identity/purity check.
• Food Analysis: quality control, additives, pesticides. Stability testing.
• Clinical Application metabolism studies, drug screening, stability
testing etc.
• Industrial Application: process development and optimization, In-
process check, validation et
• Forensic: poisoning investigation.
ADVANTAGES

• Rapid process and hence time saving


• Accuracy and Precision

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