B CNG THNG TRNG H CNG NGHIP THC PHM TPHCM

KHOA CNG NGH THC PHM MN: K THUT THC PHM III

ti: LN MEN B SU V NG HC QU TRNH LN MEN GIN ON

SVTH: Nhm 6 GVHD:Nguyn Th Thu Sang

Tp.HCM, thng 10 nm 2013

Danh sch nhm

1. L Th Lan 2. L Qu Hu 3. Gp Hng Lin 4. Hunh Nhi 5. L Trng Hoi Nh 6. L Th Trc Phng 2006110048 2006110030 2006110050 2006110075 2006110080 2006110083

7. Nguyn Quang Tra

8. H Th Thanh Thao

2006110125
2006110099

LN MEN B SU TRONG MI TRNG LONG (LN MEN CHM)

La

phng phap ph bin rng nht trong quy trinh ln men cng nghip Dung cho ca vi sinh vt ki khi v vi sinh vt hiu khi ng dung: san xut men banh mi, protein n bao, ch phm vi sinh lam phn bon, thuc tr su, cac acid amin, vitamin,

 Tn it mt bng trong xy dng v lp dt dy chuyn Chi phi in nng v nhn lc thp. D t chc c xi nghip c san lng ln D c gii hoa, t ng ha, t choang b mt so vi ln men b mt

Ln men gian oan ng hc qua trinh ln men gian oan

Nui vsv sinh trng n thanh phn cua mi trng dinh dng b gii han Khng th duy tri trang thai nay trong thi gian dai c s dung trc ht cho s ln men v trung

Culture chuyn t pha luy tha sang pha cn bng Giam dinh dng v tng sinh khi cua t bao Trang thai sinh l cua t bao cung thay i Tao thanh san phm vi trang thi sinh l nht nh

Sinh trng biu thi s tng trng thanh phn t bao

VSV sinh san bng nay chi hay phn i

T bao tng trng n mc nht nh se phn ct thnh hai t bao

VSV a nhn thi khng phn ct t bao

NG CONG SINH TRNG

Phn tich trong mi trng nui cy theo me hoc trong h thng kin Thi gian nui cy cng di cht dinh dng giam cht ph thai cng tng.

ng cong sinh trng co 4 giai

oan

Nu thi gian nui cy l truc honh v s logarit cua t bo sng l truc tung th ng cong cua 4 giai oan c th hin nh sau

GIAI ON TIM PHT (LAG PHASE)

T bao cha phn ct nhng th tich, khi lng tng Nguyn nhn: t bao gi, thiu ATP,

Thanh phn mi trng mi khng ging mi trng cu

Cn thi gian tng hp enzyme mi

GIAI ON TIM PHT (LAG PHASE)

Cc t bao b thng tn v cn thi gian hi phuc

Tinh cht hoa hc cua mi trng mi khac nhiu vi mi trng cu thi giai oan tim phat se keo dai v ngc lai

Cy vi sinh vt t giai oan tim phat hay giai oan t vong thi giai oan tim phat se keo dai

Giai on logarit hay pha ch s

- Vi sinh vt sinh trng v phn ct vi nhp ti a trong mi trng v iu kin nui cy thch hp - Nhp sinh trng cua chng l khng thay i - Do cc t bo sinh ra khc nhau rt t nn ng cong sinh trng trn nhn khng gp khuc

Giai on logarit hay pha ch s

Giai on logarit hay pha ch s

Nu cn bng dinh dng hay cc iu kin mi trng thay i se dn n s sinh trng khng ng u

S sinh trng khi nhp tng hp cc thnh phn cua t bo tng i bin ho se bin i theo cho n khi at ti cn bng mi

Giai on logarit hay pha ch s

Khi chuyn t bo cua vsv t mi trng ny sang mi trng khc
Tao ribosome Nng lc tng hp protein Tng cng tng hp protein v DNA

Tc pht trin nhanh chng

Giai on logarit hay pha ch s

T bo DNA Ti Thi gian tao cc enzyme tao phn ct cn thit sinh tng hp cc thnh phn khng c sn Sinh trng cn trong bng mi se trng Tng hp phuc hi v tr v giai protein v oan logarit ARN

Giai on logarit hay pha ch s

Giai on n nh
S sinh trng cua qun th cui cng se dng lai ng cong sinh trng i ngang

Giai on n nh
Nng vi khun vo khoang109 /ml.

Nng ng vt nguyn sinh v vi tao ch

at 106 /ml.

S lng t bo sng l khng thay i. Do

s lng t bo mi sinh ra cn bng vi s

lng t bo cht i hoc t bo phn ct

m vn gi nguyn c tnh trao i cht.

Nguyn nhn lm cho qun th VSV chuyn sang giai on n nh

- S han ch cua cht dinh dng Vd: VSV hiu kh b han ch bi nng oxygen, ch c VSV sinh trng b mt mi c u nng Oxy, - Gp s tch luy cua cc san phm trao i cht. Vd: VSV k kh ln men ng sinh ra lng ln acid lactic, lm acid ha mi trng, - S tiu hao ht ng, hay khi s lng VSV at n mt gii han nht nh th s sinh trng c th b inh ch

Giai on n nh
Nhng bin i cua vi khun khi chuyn sang giai oan n nh
Khng

c s bin ha r rt v hnh thi nhng c th thu nh kch thc lai, thng do cht nguyn sinh co lai v nhn gia m c lai thiu thc n vi khun se sinh ra mt loai protein oi lm cho t bo khng nhiu hn vi cc thng tn bng nhiu con ng khc nhau

Khi

Giai on n nh
Nhng bin i cua vi khun khi chuyn sang giai oan n nh
Chung lam tng cac lin kt peptidoglycan v s bn vng cua thanh t bao. Chng han Dps l mt loai protein kt hp vi DNA ly t cac t bao oi, co th bao v cho DNA

Phn t Chaperones can tr s bin tinh cua protein va hi phuc lai c cac protein b tn thng

Giai on n nh
Cc t bo oi c th kh b cht i, khng c vi tnh trang b oi, bin ha cua nhit , s tn thng v oxy ha v s thm thu

Tng sc khng vi cc ha cht c hai

Lm cho mt s vi khun c th sng lai sau vi nm b oi

Giai on t vong
T bo vi sinh vt b chi phi bi cc yu t sau Can kit ngun dinh dng Cc san phm trao i cht c trch luy ngy cng nhiu S lng t bo sng giam xung

y l qu trnh thch hp thu nhn san phm trao i cht (san phm len men)

Giai on t vong
Tng s t bo sng v cht khng i S lng t bo t ngt giam th tc cht chm lai

ng cong trong giai oan ny kh phc tap

Giai on t vong
xc nh c s lng t bo sng phai pha long ra ri cy ln thach a, a vo mi trng thch hp ri xc nh Kt thc qu trnh, tin hnh thu ly san phm. ng dung san xut nhiu hoat cht quan trng nh amino acid, cc cht khng sinh,

TNH TON V QU TRNH SINH TRNG

Lm c s nghin cu v sinh l hc, sinh thi hc cua vsv v giai quyt mt s ng dung trong san xut cng nghip

S lng t bo tng theo phng thc 2 . Thi gian gia hai ln phn chia lin tip l thi gian th h.

TNH TON V QU TRNH SINH TRNG

TNH TON V QU TRNH SINH TRNG

th th hin s sinh trng th h cua vsv

Cng thc tinh s lng t bao

Trong :

Nt : s t bo sau n ln phn chia trong thi gian t

N0 : s t bo ban u

n : s ln phn chia

- T cng thc trn c th bin i v tnh s th h n logarit thp phn: Log = log0 + nlog2 n= Log 0,301
log 0

- Tc sinh trng trong giai oan logarit c th biu th bng hng s tc sinh trng bnh qun k

K=

Log log0 = 0,301t

 Thi gian cn thit tng gp i tng s t bo l thi gian th h bnh qun c biu th bng g Nu t=g th = 20

Thi gian th h bnh qun l o s ca hng s tc sinh trng bnh qun

Thi gian th h bnh qun g c th cn c trc tip vo th bn logarit v hng s tc sinh trng tnh ra

th xc nh thi gian th h

Cu 1: u im nao sau y khng nm trong phng phap nui cy chim?

A. D t chc xi nghip co san lng ln B. D c gii hoa, t ng hoa C. Khng gy nhim mi trng nui cy D. Chi phi in nng va nhn lc thp

Cu 2: Pha no c vsv sinh trng v phn ct vi nhp ti a nht?

A. Pha lag B. Pha log C. Pha cn bng D. Pha suy vong

Cu 3: Thi gian pha lag di hay ngn phu thuc vo?

A. Lng ging cy v lng c cht B. Lng c cht v nhit

C. Tui ging cy v lng ging cy D. Lng c cht v tui ging cy

Cu 4: Trong qu trnh ln men gin oan, ngi ta thng thu nhn san phm trao i cht (san phm len men) pha no?
A. Pha lag

B. Pha log
C. Pha n nh D. Pha suy vong

Cu 5: Nu nui cy vi sinh vt vi muc ich thu nhn sinh khi t bo c hoat tnh sinh hc manh th nn kt thc v thu nhn san phm giai oan no l tt nht?

A. Cui giai oan n nh B. Cui giai oan logarit

C. u giai oan t vong D. u giai oan logarit

Xc nh s sinh trng cua vi sinh vt

Xc nh s lng t bo
2

Xc nh khi lng t bo

m trc tip di knh hin vi

Xc nh s lng t bo

Dng my m in t

Dng dung cu m khun lac Phng php mng lc membrane filter

Phng php tnh

A.1000.F N V
F: h s pha long. V: th tch 1 ln.

(T bo/ml)

A: s t bo trn 1 ln.

1000: h s chuyn i: 1ml = 1000mm3

Dng my m in t

ng vt nguyn sinh, vi tao, nm men.

Dng dung cu m khun lac

u im

Xc nh s lng t bo cn sng
n gian, ng dung rng ri khi xc nh vi sinh vt sng trong phn tch mu: t, nc

Nhc im

Nu vi khun dnh thnh khi th s lng m c thp hn thc t

Tnh bng n v hnh thnh khun lac: CFU

 Pha

long mu theo dy thp phn

Tch khun lac v phng php kim tra s lng vsv thng qua m khun lac mc trn mi trng thach a

 Dng

giy lc hnh trn c kch thc l nh

hn vi sinh vt
Sau

khi lc at giy lc ln thach hoc thm

c bng mi trng nui cy thch hp

Sau

24h m s khun lac trn giy lc, tnh

mt vi sinh vt trn mu

Xc nh trng lng kh cua t bo Xc nh khi lng t bo

o uc

Xc nh trng lng kh cua t bo

Ly

tm thu sinh khi t bo kh trong l sy trng lng kh

Ra Lm Cn

Phng

php ny tn thi gian, khng tht

s chnh xc

o uc
Lc

nng t bo 107 t bo/ml th dch nui vn uc, nng cang tng th uc cng tng. Lm can tr nh sng qua dch nui tn xa nh sng bng quang ph k hm lng mt s vt cht trong mt t bo l ging nhau th tng lng cht o trong t bo c lin quan trc tip vi tng s sinh khi

Nu

o uc

KT LUN
hiu c phng php ln men b su trong mi trng lng
Tm

Hiu c ng cong sinh trng cua vi sinh vt qua cc giai oan pht trin

Bit c cch tnh ton v qu trnh sinh trng cua vi sinh vt

Xc nh c s sinh trng cua vi sinh vt

Cu hi cung c
Cu 1: Trong bi bao nhiu phng php xc nh s lng t bo?
A. B. C. D.

3
4 5 C

Cu 2: Cng thc tinh mt t bao/ml bng phng phap m trc tip la:

A.
B.
C.

Cu 3: Phng php no c th xc nh c s lng vi sinh vt cn sng?

m trc tip di knh hin vi, dng dung cu m khun lac. B. Dng dung cu m khun lac, phng php mng lc. C. Dng my m in t, phng php mng lc. B
A.

Cu 4: Trong phng php mng lc, thi gian nui cy vi sinh vt l bao lu?
A. B. C. D.

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