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  • Ingeniería Metabólica: Otros Organismos

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    BrigditeZuleta
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    The document discusses metabolic engineering of lactic bacteria to produce higher levels of folate (vitamin B9) which is necessary for protein and hemoglobin formation. Specifically, it describes how genes involved in folate biosynthesis in Lactococcus lactis have been analyzed and overexpressed using the NICE system. Both individual genes and combinations of genes have been overexpressed, resulting in higher folate production. It also mentions engineering Lactobacillus gasseri to produce folate and using genes from Pseudomonas putida to engineer E. coli to produce indigo dye from the indigo plant Indigofera tinctoria.

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    GENETICA

    Original Title

    Clase 8a

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    The document discusses metabolic engineering of lactic bacteria to produce higher levels of folate (vitamin B9) which is necessary for protein and hemoglobin formation. Specifically, it describes how genes involved in folate biosynthesis in Lactococcus lactis have been analyzed and overexpressed using the NICE system. Both individual genes and combinations of genes have been overexpressed, resulting in higher folate production. It also mentions engineering Lactobacillus gasseri to produce folate and using genes from Pseudomonas putida to engineer E. coli to produce indigo dye from the indigo plant Indigofera tinctoria.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    9 views10 pages

    Ingeniería Metabólica: Otros Organismos

    Uploaded by

    BrigditeZuleta
    The document discusses metabolic engineering of lactic bacteria to produce higher levels of folate (vitamin B9) which is necessary for protein and hemoglobin formation. Specifically, it describes how genes involved in folate biosynthesis in Lactococcus lactis have been analyzed and overexpressed using the NICE system. Both individual genes and combinations of genes have been overexpressed, resulting in higher folate production. It also mentions engineering Lactobacillus gasseri to produce folate and using genes from Pseudomonas putida to engineer E. coli to produce indigo dye from the indigo plant Indigofera tinctoria.

    Copyright:

    © All Rights Reserved
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    of 10

    Ingeniera metablica

    Otros organismos

    M. Sc. WILLIAN CAPA ROBLES

    Metabolic Engineering of
    Lactic Bacteria
    Folate (B9)

    Platform
    NICE

    Platform
    NICE

    The genes involved in folate biosynthesis


    have been analysed completely.
    By genetic eng. several of these genes
    have been over expressed in
    L.lactisNZ9000 using the NICE system
    Individual gene can be over expressed or
    in combination

    Nota: El cido flico, folacina o cido pteroil-Lglutmico (la forma aninica se llama folato) o
    vitamina B9, necesario para la formacin de protenas
    estructurales y hemoglobina.

    Part of Folate gene cluster L.


    lactis cloned behind strong
    promoter

    High production of folate by over


    expression of whole fol gene
    cluster

    Folate production in engineered


    Lb. gasseri

    La planta india Indigofera tinctoria produce naturalmente Indigo


    (tinte textil azul).
    E. coli fue introducida con el gen que convierte indol en indigo de
    Pesudomonas putida con mutagenesis dirigida y controlando el
    medio.

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