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AND PROTEINS
1. Ninhydrin Reaction
Spot the solutions of amino acids provided
on a filter paper, add a drop of ninhydrin
reagent to each spot and hold the paper over
a bunsen flame to dry. Note and comment on
the colors produced.
Expected results: Amino acids give purple
colors with ninhydrin reagent. But proline (an
imino acid) gives yellow color with ninhydrin.
2. Xanthoproteic Reaction
Add 1 ml of conc. nitric acid to 1 ml of the
amino acid solution. Warm the mixture in a
boiling water bath and allow to cool. Note the
formation of precipitates and colors. Add
sufficient 40% NaOH to make the solution
strongly alkaline.
Expected result: A yellow color in acid
solution which turns bright orange in alkali
indicates the presence of one or more of the
aromatic amino acids: tryptophan,
phenylalanine, and tyrosine.
3. Millons Reaction
Add 5 drops of Millons reagent to 1 ml of the
test solution and warm the mixture in a
boiling water bath for 10 minutes. Cool to
room temperature and add 5 drops of sodium
nitrate solution.
with protein
samples, e.g., Test Sample 0.1 -