Practical

DEPARTMENT OF HISTOLOGY
MEDICAL FACULTY
UNIVERSITY OF SUMATERA UTARA
MEDAN 2010
 Steps in Tissue Processing

Fiksasi Dehidrasi Pembeningan

(Fixation); (Dehydration) (Clearing)

Pembenaman
Pemotongan Pengecoran
(Infiltration/Impre
Jaringan (Blocking/Casting
gnation/Embeddin
(Sectioning) )
g)

Pewarnaan Perekatan Pelabelan

(Staining) (Mounting) (Labelling)
 Impregnation

 Impregnation is replacement process of clearing

agent from the tissue and replacing it with
paraffin.
 Clearing agent remaining can be crystallized in a
tissue so that when cut with a microtome tissue
will tear.
 Impregnation technique :
 Paraffin/ paraplast I : 2 jam;
 Paraffin/ paraplast II : 1 jam;
 Paraffin/ paraplast III : 2 jam.
Preparation:
•Microtome knive
•Microtome blade (disposable)
• Place the knife on the microtome with a
certain angle.
• Place the blocks of paraffin to be cut on the
holder by using a hot spatula.
• Place the following block holder in place in
microtome preparations.
•Prepare coated slides:
•Albumin (putih telur);
•Gelatin;
•SuperFrost
•Poly-L-Lysine
•Waterbath contain hot water (adjust
with paraffin boiling points)
Cutting with Microtome
Sectioning Technique
 thickness + 5-10 micrometer
 Adjust the distance of preparations that are held by
the holder in the direction the blade as close as
possible;
 Move the rotor (rotation) on microtome rhythmically,
Dispose of paraffin ribbons without tissue;
After a piece of tissue, cut into blocks preparations
carefully;
 move carefully with tool, from water in the waterbath;
 After the paraffin ribbon terkembang well, stick to the
glass object paraffin;
 Keep glass objects contain pieces of paraffin and tissue
until the time to stain
Staining Process with Hematoxilin Eosin
Thank you…
 Kuis

Indonesia
1. Sebutkan cara-cara mempelajari sel!
2. Sebutkan langkah pemrosesan jaringan.
3. Sebutkan dasar pewarnaan rutin hematoxylin
eosin.

English
1. Please list the ways to study the cell!
2. Name the tissue processing steps.
3. Mention the basic for routine Hematoxylin eosin
staining.