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Rotor Is The Rotating Part of The Centrifuge Which, Has Fixed Holes That Hold The Test Tubes or Other Container of The Sample at An Angle
Rotor Is The Rotating Part of The Centrifuge Which, Has Fixed Holes That Hold The Test Tubes or Other Container of The Sample at An Angle
that hold the test tubes or other container of the sample at an angle.
Different Types of Rotors
• Swing- Bucket Rotor – it holds the sample container in a horizontal
position during centrifugation so that the tube is aligned
perpendicular to the axis of rotation and the parallel to the applied
centrifugal field.
Used to separate large sample.
The pellet is formed at the bottom of the container.
Sample Range : 36mL to 2.2 mL
Advantage: The sedimenting particle has longer distance to travel that
allow better separation and the supernatant is easier to withdraw.
• Fixed Angle Rotor – it hold the tubes at a stable angle relative to the
axis of rotation.
Used for sedimentation with high centrifugal forces of up to and
more than 60 000 x g.
Sample Range: 0.2 mL to 1 mL
Advantage: It allow shorter sedimentation time in comparison to
swing bucket rotors which mean it has higher rotational speed and
higher centrifugal force.
• Vertical Rotor - the tubes are aligned vertically to the surface or
parallel to the axis of rotation.
It has very low K factors, which is useful if the particle must only
move a short distance until it pellets.
Advantage: The time it required for the sedimenting particles to pellet
is small.