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• The latter can provide contaminant-free environment and make possible the
control of culture pH, temperature, CO2concentration in aeration gas, light
intensity, and other culture variables, hence giving a wider range of relevant
products.
Types of Photobioreactors
Type Sub-type Description
Tubular Reactor • Vertical TR Generally in the TR the microalgae culture is
• Horizontal TR circulated in transparent tubes by a centrifugal pump,
• Helical TR and passes intermittently through a degasser –i.e. an
air- sparged vessel, where the accumulated oxygen is
blown off.
Flat plate reactors Vertical FPR Vertically or horizontally oriented flat, transparent
(FPR) Horizontal FPR vessel in which mixing is carried out directly in the
reactor via air sparging
Stirred tank reactor A vertical cylindrical tank, in which agitation is
STR) provided via an impeller or magnetic stirrer (in
smaller units), every parameter can be controlled to a
higher level than in other reactor types. Illumination
can be provided internally/externally by artificial
light.1
Plastic Bags Various different Plastic bags arranged in different patterns are
patterns according installed with aerator to promote yield.
volume.
Photobioreactor for Lab Scale
• Stirred tank photobioreactor is most preferred PBR for lab scale microalgae
cultivation.
• The advantage of this type of photobioreactor over others for lab scale
cultivation is its better parameter control. This is especially important in
while growing sensitive species such as H. pluvialis.
• But this PBRs are only used for lab scale cultivation due to the problematic
light provision for all cells. The bigger the vessel is, the more critical this
factor gets. At lab scale this factor is controlled by using multiple mixers on
middle axel.
• Another photobioreactor which is used for lab scale cultivation is flat plate
reactor.
• For the lab scale cultivation of less sensitive algal species illuminated flask
and plastic bags are better option because of less capital requirement.
Major Constrains
• Light Intensity:
• Light is absolutely essential for microalgae cultivation as they use it as source
of energy and their growth is highest at optimum light intensity and growth
decreases with increase or decrease in intensity.
• Optimum light intensity for microalgae varies depending on the strain.
• For many microalgae, increase in the light intensity causes photo inhibition
because of disruption of the chloroplast lamellae growth and inactivation of
the enzymes involved in the carbon dioxide fixation.
• Optimization of light intensity during lab scale cultivation is a challenge.
• Temperature:
• Temperature influences the algae growth rate, cell size, biochemical
composition, and nutrient requirements.
• If temperature is higher/lower than the optimum -a significant decrease in
the growth rate can be observed.
• Optimum temperature for microalgae also varies depending on the strain.
• This problem can be conquered by using temperature control equipment or
by selecting proper location.
Scale up
• The aim of the scaling-up is to obtain larger cell/product quantities at a larger
scale with at least the same viability and quality of culture as obtained at a
smaller scale.
Cultivation Experiment
in flask
Laboratory Scale
Photobioreactor
Algal Strains -Algal cultures are obtained either from universities or from companies such
UTEX and Algae Analytics.
-The strains are transported and incubated at optimum temperature under
continuous light intensity.
Glassware Conical flask, Culture bottles, Measuring Cylinders, Volumetric flasks etc.
Standard Chemicals
Other Equipment's Lux meter, Air Compressor, Plastic tubing, Media and air filters, Shaker, etc.