Example of Bacterial

Biochemical Tests

Aida Nadrah bt Ahmad Junaidi

Nor Eliana bt Baizuri
Nur Syakirah bt Dzulkafli
Ummi Syahida bt Sulaiman
Tests Catalase Test Coagulase Test Spirit Blue Agar
What? • Used to detect the presence • 2 methods • A medium that contains a
of the enzyme catalase in 1. Slide coagulase test supply of lipids (eg. olive
bacteria (measure bound oil, corn oil)
 Catalase: protect bacteria coagulase)
from toxic hydrogen 2. Tube coagulase test
peroxide (H2O2) (measure free coagulase)
accumulation
 H2O2 O2 + H2O

Purpose • To distinguish among • To differentiate between • Used to identify bacteria

Gram-positive cocci pathogenic and non- that are capable of
• Differentiate obligate pathogenic members of the producing the enzyme
aerobic and anaerobic genus Staphylococcus. lipase.
bacteria

Result Presence of O2 characterized by
formation of bubbles (+ve
result)
Example • Staphylococci (catalase
+ve) and streptococci
(catalase -ve)
1. Slide : clumping of cocci
(+ve result)
2. Tube : gelling of plasma
(+ve result)
• Pathogenic strains of
Staphylococcus aureus
(+ve) and non-pathogenic
species (–ve)
• A clearing of the blue color
in the medium forming a
clear streak (+ve result)
• creates a dark blue halo
around the bacterial
growth.
• Bacillus subtilis
• Staphylococcus
Catalase Test Coagulase Test Spirit Blue Agar

Slide:

Tube: