BIOLOGY IS EASY

Paper 3 - Question 1
(a) In Table 1, list all the materials and apparatus
labelled in Diagram 1.
Material Apparatus
Water bath Thermometer
2 ml of 1 % amylase solution Boiling tube
3 ml of 1% starch solution Beaker
5 ml buffer solution
(b)Record the time taken for iodine solution to
remain yellow in Table 2.
(c) (i)State two different observations made from
Table 2.
• Two extreme observation.

• When the buffer solution at pH 5, the time taken for iodine solution to remain
yellow is 28 minutes.
• When the buffer solution at pH 8, the time taken for iodine solution to remain
yellow is 6 minutes.
(c) (ii) State the inferences from the observation in
2 (c)(i)
• Convert the value to words

• At acid medium, the rate of reaction is low

• At alkaline medium, the rate of reaction is high
(d) Complete Table 3 based on this experiment
Variable Method to handle
MV:
RV:
CV:
(e) State the hypothesis for this experiment
• A neutral medium at pH 7 is optimum for the activity of amylase