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Objectives
At the end of this lab, you will be able to perform:
• a method to detect genetic modification in food.
• a method for isolating DNA.
• a method called PCR for amplifying DNA .
• a method for separating DNA by size using agarose gel electrophoresis.
Genetically Modified Foods
Figure 9.1
BIOL1500 – Lab 10
• Goal: Compare the DNA from non GMO foods and GMO foods
PCR
Solution: Use PCR to amplify parts of the genome that contain target sequences
BIOL1500 – Lab 10
GMO – PCR
Part II
DNA Fingerprinting
TEAM
PART 2
ADD THE LOADING DYE TO THE DNA SAMPLES
Use micropipettors to add 10 µl of Loading Dye
(LD) to each DNA sample tube – make sure the
dye and sample mix and are at the bottom of
the tube
Use a new tip for each sample
LANE SAMPLE LOAD VOL
1 Non GMO PMM 20 ul
2 Non GMO GMM 20 ul
3 Test GMO PMM 20 ul
4 Test GMO GMM 20 ul
5 + control PMM 20 ul
6 + control GMM 20 ul
DNA Fingerprinting
LOAD DNA SAMPLES
INTO THE GEL
Use the micropippete
to add 20 µl of the
DNA samples into
the wells in the gel
Use new tip each time
DNA Fingerprinting
TEAM
POUR TBE BUFFER INTO THE ELECTROPHORESIS CHAMBER
START THE ELECTROPHORESIS
RUN THE DNA GEL for 30 minutes at 100 volts
Compare your
test food
With the non
GMO band
and the +
control to
determine If
your sample
food source
Contains GM
genes
BIOL1500 – Lab 10
Using DNA for Forensic Science
PCR/DNA fingerprinting