Islamic University_Gaza

Medical Technology Department

Reham Sabri Hammad

2005\0076

Supervisor :
Dr. Abdelraouf A. Elmanama

2008\2007
 Outlines

 Introduction.
 Principle Of Citrate Test.
 Significance Of Citrate Test.
 Materials and Method.
 Result.
 Summary.
 Introduction

 Tests for the ability of bacteria to convert

citrate (an intermediate of the Krebs
cycle) into oxaloacetate (another
intermediate of the Krebs cycle).
 In this media, citrate is the only carbon
source available to the bacteria.
 Principle Of Citrate Test
 To determine if an organism is capable of
utilizing citrate as sole carbon source for
metabolism with resulting alkalinity.
 If organisms utilize citrate and ammonium
salts, the medium becomes more basic and the
bromthymol blue changes from green to blue
as a result of an increase in the pH of the
media.
 If it can not use citrate then it will not grow.
 Significance Of Citrate Test

 A differential cultural test to identify

genera within the bacterial family
Enterobacteriaceae that are able to utilize
sodium citrate as a sole source of carbon.
 Materials
 Simmons Citrate agar
which contains :
1. sodium citrate (carbon
source) .
2. ammonium salts (nitrogen
source).
3. Bromthymol blue indicator.
 Inoculating needle .
 Method
1. Streak one
organism over the
surface of the agar
slant, then stab the
butt.
2. Incubate the tube
at37°C for 48 hours
.
 Result
 Examine for growth
(+) .
 Growth on the
medium is
accompanied by a rise
in pH to change the
medium from its
initial green color to
deep blue.
 Result

 Some intestinal bacteria are (+)citrate as:

Klebsiella .
Citrobacter.
Enterbacter aerogenes .

 while others is(-) citrate as: E. coli

 Summary
Klebsiella Enterbacter Citrobacter E. coli
aerogenes

(+) (+) (+) (-)

 At last, I wish that you have enjoyed my
presentation.
 Thank you, and have a nice day please.
 My best wishes
 References
 http://srv2.lycoming.edu/~diehl/321-f03/lecture2.htm
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 http://whitewolf.newcastle.edu.au/techinfo/proc_bacto_biochem.ht
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 http://science.swu.edu/~wsinnamon/micrometabolism.htm
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 http://web.fccj.edu/~lnorman/unknowns.htm?index=2
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