Measuring Pipette

By :

1. Discha Prameswara
2. Yeni Sulistyawati Pamungkas
 MEASURING PIPETTES

• They are straight glass or plastic tubes with one tapering

end.
• Calibrated into small divisions so that various amounts of
liquid can be measured with the same pipette.
• Usually used to measure any amount between 0.1ml and
25.0ml.
• They are not as accurate due to the fact that any
imperfection in their internal diameter will have a
greater effect on the volume delivered. But more
accurate than Pasteur pipettes
 Definition
• Measuring pipette are pipettes with various
volumes along the tube. They commonly
come in 5, 10, 25 and 50 mL volumes.
• Measuring pipette are also known as
Graduation pipette
 History
This instrument is also referred to as a Mohr pipette, after Karl Friedrich
Mohr, a 19th century pharmacist. Mohr is said to have developed the
graduated pipette so that he could specify the volume of liquid that he
wanted to transfer. The word "graduated" refers to the series of lines
located at specific intervals that run the length of the tube. These lines
allow the user to specify and measure the volume of liquid that is being
transferred. Graduated pipettes are more accurate than Pasteur pipettes,
but not as accurate as volumetric pipettes.
 Function
• Measuring pipettes are used for dispensing a
variable volume of aqueous liquid. Pipettes
are calibrated with distilled water. They can be
used with non-aqueous liquids but they will
not be as accurate.
 Usage Guide
• Hold pipette in solution, don’t touch the bottom.
• Squeeze bulb and attach to top of the pipette.
• Hold forefinger on top of pipette to control volume
aspiration.
• Subtract the amount needed into separate beaker
while staying eye level to assure proper measurement.
• Measure solution from bottom of the meniscuses, the
crescent shaped surface of liquid that is visible in the
pipette.
• Subtract needed volume from the initial volume and
find the volume needed to release to in order to get
the desired amount.
 MOHR AND SEROLOGICAL PIPETTES

Measuring pipettes are divided

into:
• MOHR PIPETTES
– the graduations on these
always end before the tip

• SEROLOGICAL PIPETTES
– the graduation marks
continue to the tip
Thank You