Your Baby is coming with a miracle gift to your

Family

i de tod a y
De c
re t omo r row
for a Secu
Your baby’s lifeline for nine months……Umbilical Cord blood
……..may well be your family’s life-saver against deadly diseases
CORD BLOOD – A TREASURE TROVE OF STEM
CELLS

•Residual blood present in the cord

which was once a Medical Waste

•Now A Medical Miracle!

•Cord blood collection procedure is no

harm to CHILD and MOTHER.
 WHAT IS SO SPECIAL
ABOUT
UMBILICAL CORD
BLOOD?
•It has high concentration of special type of cell
called “STEM CELL”

•If the cord blood is collected at the child’s birth

then these Stem Cells can be harvested and
stored under – 196ºC temperature for future use
Stem cells are “master” cells
Get their name from the word “stem” as the
stem of a plant.

• Tissue precursor cells that have the

ability to self-renew and differentiate
into more specific cell types. Stem cells
serve as a continuous source of new cells
• The goal of any stem cell therapy is to
repair damaged tissue that can’t heal itself
Stem Cell Differentiation
Chief Sources Of
 BEST GIFT OF THE
LIFE
Make sure that you offer your child
the disease free life and also inturn
your child makes your whole family
protected with 75 life threatening
diseases.
Stem Cells Can Protect Us From

• Blood disorders
• Leukemia's
• Cancer.
• Thalassemia
• Heart stroke
• Diabetes
• Spinal cord Injury
 BANK OF STEM CELL

• Your baby's cord blood could save the life of the

child as well as family member or someone else in
need.
• You only get one chance!
• Thousands of adults and children are in serious need
of a life saving transplant.
• The possibility of medical advancement relies on
cord blood stem cells.
• A Joint Venture between Cryobanks
International Inc & RJ Group, India
• R J Group is 15000 crore Group

RJ Corp

Health
Beverages Hospitality
Real Estate Education Care
North & South America Europe/Russia/Mid East Asia
43 Systems 33 Systems 47 Systems
23 Cord Blood Banks 26 Cord Blood Banks 27 Cord Blood Banks
5 Countries 17 Countries 7 Countries
 CRYOBANKS INTERNATIONAL
INDIA
• First Cord Blood Bank which is licensed by Drug
Controller General of India& ISO Certified
• 28.000 sq. ft. area with 14,000 dedicated laboratory space
• Provides complete in house processing and storage
technology, licensed by Cryobanks Intl. Inc. USA which is
accredited by AABB, US FDA, FACT NETCORD and
NMDP

The Caitlin Raymond

International Registry
 CRYOBANKS INTERNATIONAL
INDIA
• Closed system of processing which prevents chances of
contamination of cord blood & High Viability counts
in each cord blood unit processed.
• Lab is completely class 10,000 – sterile laboratory
premises.
• National coverage – We collect your unit from all
major cities in India
• Our highly qualified team of medical professionals has
been extensively trained and certified in cord blood
technology
• Our lab operates 24X7We have several flexible
payment plans to help you protect your family
 Advantages of Cryobanks

• Stem Cells insured with Bajaj Allianze General insurance co.

• HLA matching facility available in Gujarat
• Import export licensed for transferring the sample out of India and
vice a versa.
• Dr. Sunil Advani (Hematologist, Mumbai) will be heading the
transplant department at Medicity Gurgoan from January 2009.
• Doctor is available at all center of Gujarat
• Transplant facility and transplant doctor is available in
Ahmedabad
CRYOBANKS INTERNATIONAL
INDIA
GURGAON CENTER

ADDRESS

129, PACECITY 1,
SECTOR 37
GURGAON INDIA
TEL. 0124-4265030
FAX: 0124-4370257
TOLL FREE NUMBER
1-800-102-2796
E-MAIL
cryo@cryobanksindia.com
Dr.Naresh Trehan
(M.D.)
CARDIOLOGIST
STEMCELL
SPECIALIST
FOR CARDIAC
SURGERY

In the words of Dr Trehan, “Umbilical Cord Blood

contains millions of valuable stem cells that
can be used in many different ways to correct leukemia,
anemia, metabolic disorders, and many white blood cell
diseases. In the very near future, these cells could be used
to regenerate heart muscles and impact neurological
disease treatments,”
“It is the future of medicine.”
Cryobanks International India Pvt. Ltd.
Staff Members (Trained at U.S.A)
Our kit is UN certified which means it can travel
anywhere in the world
 Collection procedure

Pre Collection Set

up

The Placenta is examined

for gross abnormalities
Continue

The Cord is cleaned

with an absorbent
gauze followed by
alchohol

The Cord is cleaned

with iodine at the
venipuncture site
Continue

The umbilical vein is

punctured with the
bevel of the needle
toward the vein

Blood drains by
gravity into a
collection bag
Continue

Tubing is clamped and Complete blood bag label

needle cut off and affix
Continue

Place labelled blood bag

and maternal blood into
plastic kit boxes

The box is then sealed and

stored at room
temperature
DO NOT
Incubator
Cord Blood Processing
Separator Unit for
Stem Cell
Separation of Stem
Cell in close system
Cold centrifuge
 Test Performed on Tests Performed on
Maternal Blood Cord Blood

• HIV (I & II) •TNCC-Total Nucleated Cell

• HBsAg Count
• Anti HCV •Viability
• Malaria •CD34+ Count
• Syphilis •Cord Blood grouping
• Anti HTLV (1&2) •Cord Blood culture for microbial
• Anti CMV IgM & IgG contamination
• Maternal Blood Grouping
 Coulter for in-house
diagnostic testing of the
cord blood unit &
mother
 Flow cytometer for
cd34+ counts of stem cell
unit
In house facility of all
diagnostic testing of
unit
Decreasing
refrigerator
 Vapour Phase
LN2 Storage Tanks
Storage system of stem
cell in ln2 tank
How Long Stem Cell can be stored

• Stem Cell can be stored for 21 years as per the

initial contract with the company.

• Storage can be renewed after 21 years

• Stem cell can be stored for indefinite time.

 Likelihood of HLA match
• Identical twin with same genetic complement - no
match required
• Sibling of same parents – 1 in 4 chance of 100%
match
• 3/6 or 4/6 match sufficient for transplant
• ANYONE ELSE IN THE FAMILY- no predictions
possible - similar to unrelated donors
FIRST CORD BLOOD STEM CELL TRANSPLANT
IN THE WORLD- A REALITY !

1988 in France
Eliane Gluckman

first successful related UCB transplant, of sibling

in a 6 year old boy to cure Fanconi’s Anemia

The boy is now a healthy teen

 HEMANT BEBERA-
The First Cord Blood Stem Cell Transplant
in India

Has two birthdays.

Has conquered thalassemia,
Was trested on August 7, 2002 Army
Hospital (Research and Referral),
New Delhi
 Metachromatic Leukodystrophy—
MLD

Source of stem cells: matched cord

blood >3x10E6 CD34+ cells. Need to
minimize GvHD.

Complications:
1. Severe gut GvHD, >2 months of very
extensive diarrhea, extensive nutritional
support for months.
2. Now almost 2 years from time of
transplant, perfectly normal, no signs of
any GvHD or MLD.
Metachromatic Leukodystrophy—MLD
1st child in family with juvenile MLD
Presented with signs/symptoms at age 4-5; died age 12.
2 subsequent pregnancies screened; were positive &
terminated

4th pregnancy said to be negative. Subsequent testing

after birth showed that initial testing was false negative.
Decision made to proceed with transplant prior to the
onset of any signs and symptoms. Genetic diseases
generally run “true” in families, meaning that we should
anticipate onset of symptoms at about the same time as
the sibling who died.
Myelodysplastic Syndrome
Transplanted in 2nd remission
1. Source of stem cells: No matched sibling
available so cord blood used at the center at
which he was transplanted. Use of cord blood
helped expedite transplant.
2. Delayed engraftment 3-4 weeks.
3. Initial Transplant hospitalization: fungal
infection
4. left hospital within 1 week with no recurrence of
illness

Transpl. 7:395-399, 2005

Blackfan-Diamond Syndrome or congenital absence of
erythropoiesis.

1. Chronically steroid dependent

2. Short stature, pseudotumor cerebri, osteoporosis, multiple
fractures, AVN, infection risks, iron overload neg.
3. Stem cell source: cord blood to minimize GvHD.
4. Mother pregnant: stem cells collected at birth of sibling
5. Stem cells used: cord blood 1x10E6 CD34+cells/kg plus
marrow 2x10E8 nucleated cells/kg; 100% chimerism

6. GvHD: skin, resolved. Steroids gone after 6 months.

7. Outcome: fractures healing, on growth hormone, attending
school, playing soccer, no infections.
 Genetic Immunodeficiency
Disease*
Transplanted for WAS
At age 18 months

Patients with SCID, CID,

CVID, Wiscott Adrich
Syndrome, Leukocyte
Adhesion deficiency, etc.
recover full immune function
(both T and B cells) after
unrelated cord blood
transplantation.

Shown at 3 years
post transplant
 Metabolic Disease MPS-1 (Hurler
Syndrome)*
Transplanted with UCB at
5 months of age
CB transplant arrests
progression and reverses
damage in the cornea,
liver, bones, cartilage,
hearts and brains of
young patients with
Hurler Syndrome which
otherwise causes death
by 5-6 years of age.
Shown at 6 years
post transplant
IQ147
 Genetic Disease
Sickle Cell Anemia & Thalassemia
Unrelated CB Tx at
2.5 months Both related and unrelated
cord blood transplants can
successfully treat
children with
hemoglobinopathies,
correcting the defect. If
the transplant
Is performed early in life,
survivals are >80%.

4 years Post Transplant

for Thalassemia
 Transplanted at 3 weeks of age

Metabolic
Disease/Krabbe Disease
(Globoid
Leukodystrophy)

This child is now neurologically

normal at age 5. Two year old
affected sibling died at age 2.
 The Blood Brothers-
Nicholas and Nathaniel
Henderson
 CCMB, LVPEI and Sarojini Eye Hospital
Wednesday, March 02, 2005
CCMB eyes on creating cornea in-vitro

• 250 patients who had undergone stem

cell therapy - 70 % had vision restored.

• The next step –Develop a hemi-cornea

• Final goal- Reconstruct an entire

cornea from a suitable stem cell source
TIMES NEWS NETWORK- SATURDAY, FEBRUARY 26, 2005

1. Cardiomyopathy
2. Sroke patient
3. Insulin-dependent diabetes

have been given stem cell therapy at AIIMS

As of now , 360 patients have

undergone this Therapy,
says Director of AIIMS
….. Dr. Venugopal
Transendocardial, autologous Stem Cell transplantation of severe,
chronic IHD- Circulation 2003, 107 :2294 - 2302

Severe Heart Failure Treatment

35 cardiac patients who only had chances Of

surviving after a heart transplant instead
Stem Cell therapy were given and all patients
survived.
64% of their dead heart muscles were revived
in 18 months
Spinal cord Injuries

Paralyzed Woman Walks Again

November 28, 2004

Hwang Mi-Soon
South Korea
• Korean work using cord blood stem cells
• A year following surgery for her spinal
injury, Kim Gould has some sensation in
her legs.
26 patients operated so far
 Applications in Plastic Surgery

 Fillers
 Breast implants
 Reconstruction of bone, cartilage
 Skin graft- in burns and other skin diseases
 Baldness
 Growth in Umbilical
Umbilical
Cord
Cord Blood
Blood
Stem
Stem Cell
Cell Therapeutic
Use
Use

8000
7000
6000 Annual
Cord Blood Stem Cell
5000 Transplants
4000 (Worldwide)

3000
2000
1000
0
1994 1995 1996 1997 1999 2001 2003 2005 2006
Perlow JH. Stem Cell Reviews; November 2006
Increasing use of
cord blood in
therapy
 Huntington’s
Alzheimer’s Parkinson’s
Stroke

Congenital heart disease Hypoparathyroidism

Pulmonary embolism
Diabetes
Hepatic Failure Hemophilia

Pain
Fertility
CNS Applications
Cardiovascular Applications Peripheral Vascular
Endocrine Metabolic Applications disease

Cell Transplantation
 Stem Cell therapy centres in India

o Gujarat Cancer & Research Institute, Gujarat

o Tata Memorial Hospital, Mumbai
o Adyar Cancer Centre, Madras
o Apollo Specialty Hospital, Madras,
o Apollo Hospital, Global Hospitals, NIMS, Hyderabad
o Christian Medical College, Vellore
o Narayana Hruduyalaya , Bangalore
o R&R Army Hospital, New Delhi
o AIIMS , New Delhi
o Inlaks Hospital, Pune
o Armed Forces Medical College, Pune
o Sanjay Gandhi PGIMS, Lucknow
Diseases Treatable by
Stem Cell Transplant

– Hematologic malignancies
• Leukemia, lymphoma
– Immunodeficiency diseases
• SCID, CID, CVID, WAS
– Bone marrow failure syndromes
• Fanconi Anemia, Severe aplastic anemia
– Hemoglobinopathies
• Sickle Cell Anemia
• Thalassemia Major
– Inborn errors of metabolism
• ADL, MLD, GLD
• MPS I, II, III, VI
• Tay Sachs Disease
 Diseases Treatable by Stem
Cell
Transplant
• Acute Lymphoblastic Leukemia
(ALL)
• Acute Myelogenous Leukemia
(AML) 
• Acute Biphenotypic Leukemia
• Blasts (RAEB)
Acute Undifferentiated Leukemia
• Chronic Myelogenous Leukemia
(CML)
• Advanced Chronic Lymphocytic
Leukemia (CLL)
• Juvenile Chronic Myelogenous
Leukemia (JCML)
• Juvenile Myelomonocytic
Leukemia (JMML)
• Refractory Anemia (RA)
• Refractory Anemia with Ringed
Sideroblasts (RARS)
• Refractory Anemia with Excess
• Refractory Anemia with Excess
Blasts in Transformation (RAEB-
T)
• Chronic Myelomonocytic Leukemia
(CMML)
• Aplastic Anemia (Severe)
• Fanconi Anemia
• Paroxysmal Nocturnal
Hemoglobinuria (PNH)
 Diseases Treatable by
by Stem
Cell
Transplant
Transplant
• Acute Myelofibrosis
• Agnogenic Myeloid Metaplasia
(myelofibrosis)
• Polycythemia Vera
• Essential Thrombocythemia
• Non-Hodgkin's Lymphoma
• Hodgkin's Disease
• Prolymphocytic Leukemia
• Chediak-Higashi Syndrome
• Chronic Granulomatous Disease
• Neutrophil Actin Deficiency
• Reticular Dysgenesis
• Mucopolysaccharidoses (MPS)
• Hurler's Syndrome (MPS-IH)
• Scheie Syndrome (MPS-IS)
• Hunter's Syndrome (MPS-II)
• Sanfilippo Syndrome (MPS-III)
• Morquio Syndrome (MPS-IV)
• Maroteaux-Lamy Syndrome (MPS-VI)
• Sly Syndrome, Beta-Glucuronidase
Deficiency (MPS-VII)
• Adrenoleukodystrophy
• Mucolipidosis II (I-cell Disease)
• Krabbe Disease
• Gaucher's Disease
• Niemann-Pick Disease
• Wolman Disease
• Metachromatic Leukodystrophy
Diseases Treatable by
Stem
Stem Cell
Transplant
Transplant
• Familial Erythrophagocytic
Lymphohistiocytosis
• Histiocytosis-X
• Hemophagocytosis
• Beta Thalassemia Major
• Pure Red Cell Aplasia
• Ataxia-Telangiectasia
• Kostmann Syndrome
• Leukocyte Adhesion Deficiency
• DiGeorge Syndrome
• Bare Lymphocyte Syndrome
• Omenn's Syndrome
• Sickle Cell Disease
• Severe Combined
Immunodeficiency (SCID)
• SCID with Adenosine Deaminase
Deficiency
• Absence of T & B Cells SCID
• Absence of T Cells, Normal B Cell
SCID
• Common Variable
Immunodeficiency
• Wiskott-Aldrich Syndrome
• X-Linked Lymphoproliferative
Disorder
• Lesch-Nyhan Syndrome
• Cartilage-Hair Hypoplasia
• Glanzmann Thrombasthenia
• Osteopetrosis
• Amegakaryocytosis / Congenital
Thrombocytopenia
 Emerging therapies

• Heart Disease
• Diabetes
• Muscular Dystrophy
• Multiple Sclerosis
• Alzheimer’s
• Parkinsonism
• Spinal Cord Injuries
• Lupus
 Steps To Follow :-

• The Registration Process:-Paper Work and Payment

• Pre-Collection:-Cord Blood Collection and Transport Kit
• On The Way to the Hospital:-Make a Phone Call to
Cryobanks Relationship Executive
• Collection Process:-The doctor in charge performs the
collection process
• Shipping of Cord Blood:- Responsibility of Cryobanks
Relationship Executive
• Processing and Storage
Thank you for Choosing

CRYOBANKS
Banking your hopes

India