Single Beam

Spectrophotometer
b

Read put
LS WS Io I
D Display
Light Wavelength Sample Light
Source Selector Holder Detector
(Monochromator) (Cuvette)
 Double Beam
Spectrophotometer

LS WS D I
Light Wavelength Reference Detector Capture
Source Selector Holder
b
Display

Sample
D Io

Detector
Holder
 3+ -2e- 5+
E Oxidation
E
(loss of e-)

+2e-
5+ E 3+
E Reduction
(gain of e-)
𝐴𝑏𝑠𝑜𝑟𝑏𝑎𝑛𝑐𝑒
𝜆𝑚𝑎𝑥

400 𝑊𝑎𝑣𝑒𝑙𝑒𝑛𝑔𝑡ℎ 𝜆 𝑖𝑛 𝑛𝑚 900

White WS Blue
Absorbs all color
except blue
 Serial Dilution

dilute dilute

Solution F Solution G Solution H

(50,000 ppb) (500 ppb)
Stock Solution Working Phosphate
Solution
 g
A. W. of Phosphorus ( ቁ
g of KH2 PO4 = g of KH2 PO4 mol
g
M. W of KH2 PO4 ( ቁ
mol
g
136.09
mol
g of KH2 PO4 = 0.2197g g
30.97
mol

g of KH2 PO4 = 0.049997 g

0.049997 g = 50,000 μg / L or ppb

 C1 V1 = C2 V2
50,000μg /L 0.010L C2 (1𝐿)
=
C2 (1𝐿) 1𝐿