Professional Documents
Culture Documents
Presented by
Mohini A Salunke
The medicinal plants are in use for the treatment of various diseases since
ages.Therapeutic properties are present in various parts of the plant such
as the leaf, bark, roots, stem, flowers, seeds, and fruits.
1) Legal’s test
Add a mixture of pyridine and sodium nitroprusside to the plant extract.
Then, add sodium hydroxide. The solution will turn deep red in colour.
2) BalJets test
Treat the plant extract with a solution of sodium picrate. The mixture turns yellow
ish-orange in colour indicating the presence of a lactone ring.
Glycosides
Glycosides are compounds formed from a simple sugar and another non-sugar compound by
replacement of the hydroxyl group in the sugar molecule. Glycosides can be detected in plants by two
methods:
1) BalJets test
Treat the plant extract with a solution of sodium picrate. The mixture turns yellowish-orange in colour
indicating the presence of glycosides.
2) Keller-Killani test
Add few drops of ferric chloride and 1 ml of glacial acetic acid to the plant extract.
Slowly add concentrated sulphuric acid to the above mixture.
If glycosides are present, a reddish brown ring will appear at the intersection point of the two liquids
Tannins
Tannin is a yellowish coloured derivative of gallic acid. It is bitter tasting organic substance
found in the galls, barks, and other plant tissues. Detect the presence of tannins with
these two tests:
2) Gelatine test
Take 1% of gelatine solution containing 10% sodium chloride.
Add few drops of it to the plant extract.
A white precipitate at the bottom of the test tube confirms the presence of tannins.
Proteins
2) Shinoda test
Prepare an alcoholic solution of the plant extract.
Add some pieces of magnesium ribbon to the alcoholic solution.
Add concentrated hydrochloric acid drop by drop.
Magenta colour of the solution confirms the presence of flavonoids.
Triterpenes