Professional Documents
Culture Documents
Introduction
Introduction
Fermentation Technology
Courtesy of
Prof. Dipl. Ing. W. Storhas
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Definisi
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Applications of Fermentation Technology
Medicine Environment
Chemistry Agriculture
Intermediate Renewable
Products Material
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Ferm-Tech STO 04/10
History
sterile
Bioreactors, modern development
sterile, safety requirements
Down Stream Processing
unsterile
Fermenting vessel,
unsterile rec. h-Proteins
Fermenting vessel Destillation equipment. Antibodies
unsterile Hormons
Sera
Vitamin C Cortison
Yeast for feed Penicillin
Yeast for beaker Glutamat
beer, wine, acetic acid spirit of wine Lactic acid Cephalosporin
year Pasteur
6000 ago 1000 ago (255 ago) 100 ago to day
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Ferm-Tech
Fermenting
Vessel
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Ferm-Tech
Fermentasi bir
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Ferm-Tech
7
Ferm-Tech
Amino acids:
L-Lysine, L-Glutamate acid, L-Isoleucin,
L-Metheonine, L-Asparganis acid, L-Alanin, L-Valin,
L-Phenyalanine, L-Troyptophane
Biopolymers/Catalysts: Biomass/Proteins:
Xanthane, Glucane, Dextrane, Baker Yeast, Albomen, Enzymes
Immobilised Enzymes (Invertase) Single Cell Protein (SCP)
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Ferm-Tech
Baker industry:
yeast with continuous CO2-production --> safes time
Breweries:
Preservatives, fast strengthening, L-lactic-acid, acetic acid,
gluconic acid, increasing of yield by consuming polysaccharides,
direct production of light-beer, stabile foam formation
Butcher industry:
starter cultures, bacteriocine from lactic acetic bacteria,
protection for early perish
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Ferm-Tech
10
Ferm-Tech
11
Ferm-Tech
Biotobic source
desired cells live naturally
e.g. Economic potential!??!
Lacto bacillus for
D-/L-Lactic acid - milk
I like
I don´t Glucose
like very
Lactose much
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Ferm-Tech
Screening (traditional)
mutation selection
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Ferm-Tech
Screening (modern)
Reaction rate
Check: Better economy
Product quality
15
Ferm-Tech
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Ferm-Tech
Example
classic:
Mold:
Ashbya
gossypii,
early
Riboflavin
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Ferm-Tech
Example
classic:
Mold:
Ashbya
gossypii,
late
Riboflavin
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Ferm-Tech Engineer in Bioprocess STO 04/10
Example
classic:
Mold:
Ashbya
gossypii,
late
Riboflavin
Mannheim University
of applied Sciences
19
Ferm-Tech
Example
modern
using:
DNA structure
base sequences
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Ferm-Tech
separated
fragments
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Ferm-Tech
Example
modern:
Escherichia coli
TNF
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