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    The document describes the materials and steps needed to perform a Gram stain test in a microbiology laboratory. The key materials include bacterial cultures, staining solutions, glass slides, a microscope, and lenses. The process involves staining and examining a bacterial sample under a microscope to determine if it is Gram-positive or Gram-negative.

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    The document describes the materials and steps needed to perform a Gram stain test in a microbiology laboratory. The key materials include bacterial cultures, staining solutions, glass slides, a microscope, and lenses. The process involves staining and examining a bacterial sample under a microscope to determine if it is Gram-positive or Gram-negative.

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    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
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    24 views14 pages

    BDJSB

    Uploaded by

    Nora
    The document describes the materials and steps needed to perform a Gram stain test in a microbiology laboratory. The key materials include bacterial cultures, staining solutions, glass slides, a microscope, and lenses. The process involves staining and examining a bacterial sample under a microscope to determine if it is Gram-positive or Gram-negative.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 14

    Microbiology

    laboratoric test
    Gram Stain
     Material
     solutions of crystal violet,  microscope
    Gram’s iodine (2 g potassium
    iodide in 300 ml distilled water  lens paper and lens cleaner
    plus 1 g iodine crystals), 95%  immersion oil
    ethanol and/or  Hyphomonas
     isopropanol-acetone mixture (Hyphomicrobium) neptunium
    (3:1 v/v), and (ATCC 15444) grown in
     safranin marine broth (Difco)
     Bismark brown stain (for color-  slide warmer
    blind students)  staining rack
     clean glass slides  Bacto Gram Stain Reagents
     inoculating loop from Difco for the three-step
     Bunsen burner Gram stain
     bibulous paper
    Gram Stain
    Gram Stain
    Gram Stain
    Gram Stain
    Gram Stain
    Gram Stain
    Gram Stain
    Gram Stain
    Gram Stain
    Gram Stain

    Examination the preparation under microscope ( x100) for confirmation


    Microscopic result for Gram Stain

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