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CHAPTER IV

RESULTS AND DISCUSSION

A. Observation Result
No Painting Observation Result Magnification Information
1. Negative Has a shape
Painting Magnification like wine and
40x transparent

Staphylococcus
aureus bacteria
2. Simple Cylindrical
Painting and blue
Magnification
100 x

Basillus subtilis
bacteria
3. Gram painting Shaped like
grapes and
Magnification purple
100 x

Staphylococcus
aureus bacteria

CHAPTER III
METHODOLOGY
A. Time and Place
Date/ Time : Thursday / 1 Oktober 2020
Time : 07.30-09.10 WITA (session 2)
Place : Biology Laboratory 2nd Floor West FMIPA UNM
B. Tools and Materials
1. Tools
a. Object glasses (6 pieces)
b. Ose round (1 piece)
c. Bunsen (1 piece)
d. Dropper pipettes (6 pieces)
e. Microscope (1 piece)
2. Materials
a. Spiritus (to taste)
b. 70% alcohol (to taste)
c. Culture of Staphylococcus aureus (as needed)
d. Culture of Bacillus subtilis (to taste)
e. Nigrosin solution (1-2 drops)
f. Methylen blue solution (1-2 drops)
g. Huckers solution - crystal violet (Gram A) (2-3 drops)
h. Mordan lugol-iodine (Gram B) solution (2-3 drops)
i. Alcohol Solution - 1: 1 acetone (Gram C) (2-3 drops)
j. Safranin Solution (Gram D) (2-3 drops)

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