CHAPTER III

PRACTICUM METHOD

A. Time and Place

Date/ Time : Thursday / 1 October 2020
Time : 09.10 – 10.50 WITA (session 2)
Place : Biology Laboratory 2nd Floor West FMIPA UNM
B. Tools and Materials
1. Tools
a. 1 bottle of culture
b. 1 piece of scale
c. Blend 1 pair
d. 1 piece pot
e. Spoon 1 piece
f. 1 basin
2. Materials
a. Brown sugar 50 gr
b. Ambon banana 200 gr
c. 1 pack of jelly
d. 70% alcohol to taste
e. Yeast 5 gr
f. 1 piece of napkin
g. 1 piece of sponge
C. Work Procedure
1. Preparation of culture media

Sterilize the tools Drying the culture

Prepare tools in
that will be used container
advance such as
using running water
culture containers,
and sunligth then dry
sponges, and napkins
and sprayed with
alcohol
2. Making culture medium

Cut the weighed The chopped Cut brown sugar into

Ambon banana into banana is blended thin strips and put it
small pieces and put it until smooth in a bowl with the
in the blender. blended banana

Cook bananas Put it in the pot of

Once evenly
and brown ingredients that is in
distributed, mix the
the basin
yeast that has been sugar
melted and also add
1 packet of agar

Then the dough is After that, the

stirred until evenly medium that has Add paper napkins
distributed and been made is to the culture
boils inserted into the container
culture medium that
has been tucked up
to ¼

Then cover the

culture container
with a sponge,
and the culture
medium can be
used
 CHAPTER IV
RESULTS AND DISCUSSION

A. Observation Result
No Observation image Information
1. a cork stopper
2. culture bottles
3. paper napkins
1. 4. medium