and the

relationship
between Asian
and tropical
American species
1
Over
view 01 02
ABSTRAC INTRODU
T CTION

03 04
RESULTS
DISCUSSI
ON
2
 ABSTRACT

Test the monophyly of Spathiphyllum in a clade:

the genus Spathiphyllum
Holochlamys – nested
within Spathiphyllum
Results:
Spathiphylleae as sister Spathiphylleae – sister to
of Monsteroideae the remaining species in
Monophyly of the genus
Spathiphyllum 3
 INTRODUCTION
Data in the Data in the
1900s: 2000s:
Molecular data, 3 geographically
Homalome
new fossils, disjunct genera:
and
biogeographi Homalomena,
Schismatoglottis, Schismatoglo
cal studies ttis are no
and
Spathiphyllum longer
disjunct.
4
 INTRO
DUCTI
Genera Distribution ON Neotropical species

3 Neotropical species
America: 3 species
Schismatoglottis Genus: Philonotion
Asia: 150 species
Tribe: Philonotieae

Plausible options:
America: 10 species 1. Genus: Adelomena
Homalomena
Asia : 500 species 2. Combination of Homalomena and
Philodendron
5
 INTRODUCTION
Spathiphyllum
together with
Holochlamys
Tropical form the tribe Spathiphyllum
America – 50 Spathiphylleae in is divided into
species the subfamily four sections
Monsteroideae based on the
Southeast fusion of the
Asia – 3 perianth
species segments

6
 INTRODUCTION
Spathiphyllum
together with
Holochlamys
Tropical form the tribe Spathiphyllum
America – 50 Spathiphylleae in is divided into
species the subfamily four sections
Monsteroideae based on the
Southeast fusion of the
Asia – 3 perianth
species segments

7
 INTRODUCTION
Spathiphyllum
together with
Holochlamys
Tropical form the tribe Spathiphyllum
America – 50 Spathiphylleae in is divided into
species the subfamily four sections
Monsteroideae based on the
Southeast fusion of the
Asia – 3 perianth
species segments

8
 INTRODUCTION
Massovia
perianth segments Dysspathip
completely connate a cup
around the pistil
hyllum
free perianth
segment

01 02 03 04

Amomoph Spathiphyll
yllum um
free perianth free perianth 9
segment segment
 INTRODUCTION
Massovia
perianth segments Dysspathip
completely connate a cup
around the pistil
hyllum
free perianth
segment

01 02 03 04

Amomoph Spathiphyll
yllum um
free perianth free perianth 10
segment segment
 MATERIALS AND
Taxon
METHODS DNA
Sequencing of four
plastid markers
sampling extraction

1 2 3
Purification of PCR
products Molecular Phylogenetic
analysis analysis

11
4 5 6
 MATERIALS AND
METHODS
Sequencing
Taxon DNA of four
sampling extraction plastid
markers

1 2 3

12
 TAXON
SAMPLING
Sequenced seven accessions of the genus
Spathiphyllum from:
Tropical America: S. commutatum

Tropical Asia: S. solomonense and

one accession of Holochlamys becarii

S. phryniifolium were obtained from GENBANK

They used representatives from all ten additional Monsteroideae

genera, plus six other aroids as outgroups.
13
 MATERIALS AND
METHODS
Sequencing
Taxon DNA of four
sampling extraction plastid
markers

1 2 3

14
 DNA
EXTRA
CTION
● DNA was extracted
from silica-dried
plant material using
the DNeasy Plant
Mini Kit. (Qiagen,
Valencia, CA, USA).
● CTAB method

15
 MATERIALS AND
METHODS
Sequencin
Taxon DNA g of four
sampling extraction plastid
markers

1 2 3

16
 SEQUENCING OF
FOUR PLASTID
MARKERS
matK – or Maturase K is a plant plastidial gene;
involved in Group II intron splicing.

○ Plastidial gene- part of plastid genome: encode

either components of the photosynthetic
apparatus or of the transcription/translation
apparatus of the organelle
rbcL- RuBisCo Large Subunit - encoded by the
chloroplast DNA in plants; can be contrasted with
rbcL gene of other plants in order to determine
genetic similarities or differences 17
 SEQUENCING OF
FOUR PLASTID
MARKERS
trnC-petN intergenic spacer – a gene located within
the trnC-trnD region of the chloroplast; used to amplify
specific primers to produce more genes of a specific
organism.

○ Intergenic spacer - is a region of non-coding DNA

between genes, extensive, and include repetitive
partial DNA
ycf1––which
a gene comprises a significant
cluster within fraction of
a DNA fragment
with thegenomic
potentialDNA
to be a natural product biosynthetic
gene
18
 MATERIALS AND
METHODS
Purificatio
n of PCR Molecular Phylogenetic
products: analysis analysis
ExoSapIt

4 5 6
19
 MATERIALS AND
METHODS
Manually Manually
editing of checking of
sequences sequence
Purification alignments
of PCR Molecular Phylogenetic
products: analysis analysis
ExoSapIt

4 5 6
20
 MATERIALS AND
METHODS MrBayes
3.1.2 on
RaxML 7.0 XSEDE

Purification
of PCR Molecular Phylogenetic
products: analysis analysis
ExoSapIt

4 5 6
21
 RESULTS
DNA of each
representative were
sequenced to identify Higher identical sites
similarities between between base pairs of
them and aligned; species = similar DNA due
total alignment to Restriction
length is 4970 bp Endonucleases which cuts
(base pairs) long DNA molecule at a specific
All individual and nucleotide sequence to
combined analysis produce discrete DNA
show that tribe fragments
Spathiphylleae is
monophyletic •Low variation is common 22
Spathiphylleae is within the genera of the
sister to subfamily of Monsteroideae
 RESULTS
one accession of S.
Genus Spathiphyllum cannifolium (section
that is represented Massowia) along with
by S. Genus Section
cochlearispathum, S. Holochlamys is Amomophyllum are
phyriniifolium and S. nested within sister to the clade
wallisii is sister to the Spathiphyllum containing
remaining tribe in a clade with Holochlamys;
Spathiphylleae the Asian however, support for
species this last 2 clades is
low 23
 DISCUSSION
CONCL
USSIO
Spathiphylleae - a
sister to the rest of
N
Monsteroideae
EVIDE
Synapomorphies defining
NCES
Spathiphylleae
Monsteroideae
than

CLAIM  pollen zonate

 spathe soon deciduous
Spathyphylleae is with a distinct basal
abscission
a monophyletic
 large trichosclereids but
24 tribe not in bundles

Monsteroideae’s
Figure 1. Maximum likelihood tree from RaxML using four plastid markers (matK, rbcL,
trnC-petNspacer, and partial ycf1). Bootstrap supports >75 and Bayesian posterior
probabilities >0.85 are shown on the branches.
 DISCUSSION
Markers suggest the position of H. becarii
Holochlamys and Massowia similarities
Holochlamys and Massowia differences

Lack of adequate resolution within this

clade
More data needed before proposing any
taxonomic changes
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Thank youuuuuu <3

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