WELCOME

Md.Shahadat Hossain
Lecturer
Dept. of Parasitology
Developmental Stages of
Trypanosomes
 Developmental stages of Trypanosome
1) Trypomastigote stage (Trypanosome stage)
 A blade like form with a
 Kinetoplast and blepharoplast lie posterior to the nucleus.
 Well developed undulating membrane with a free flagellum.
 Usually in vertebrate host but may also found in arthropod as
infective stage for vertebrate host.
2) Epimastigote stage (Erithidial stage)
 The Kinetoplast and blepharoplast lie anterior to the nucleus
 Short undulating membrane
 The stage found in arthropods but in few species present in
vertebrate as developmental cycle.
Developmental stages of Trypanosome(cont.)
3) Promastigote stage (Leptomonad stage)
 The Kinetoplast and blepharoplast are at the anterior tip of the
body.
 No undulating membrane
 Found in arthropod.

4) Amastigote stage (Leishmania stage)

 Rounded body
 Flagellum absent or represented by a short fibril
 Kinetoplast present.
 Found in both vertebrates and arthropods.
AMSTIGOTE
Amstigote
Epimastigote
Trypomastigote
 Points Amastigote Promastigote Trypomastigote Epimastigote
1. Shape Spherical in shape - Leaf like -
1. Kinetoplast Kinetoplast is located Kinetoplast is located Kinetoplast is located Kinetoplast is located
near the nucleus as on the anterior end of on the posterior end of centrally, anterior to
dark staining body the body the body nucleus

1. Flagellum No free flagellum Free flagellum present Flagellum emerges Free flagellum emerges
from the posterior end from the middle of the
& then folds back & body
finally emerges through
anterior end of the
body

1. Undulating Absent Absent Present Present

membrane

1. Basal body Present - Present Present

1. Occurrence Invertebrate & Invertebrate & plants Invertebrate & Invertebrate host
vertebrate host vertebrate host