Anion Exchange Chromatography

IN
Binding Buffer – 378ml
Elution Buffer – 882 ml

OUT IN
Biomass – 137.8 kg pDNA - 2.4 kg
Binding Buffer – 378 ml Biomass - 237.6 kg
Eluting Buffer – 882 ml

• Binding buffer (1.0 M NaCL + 50mM MOPS)

• Elution buffer (1.25 M NaCL +50mM Tris-HCL)