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DESCRIPTION
PROTOCOL
i-Master mix PCR Kit contains two tube system. Taq PCR tube of i-Master mix
PCR Kit contains Taq DNA polymerase and chemical stabilizer. The other 1. Add template DNA and primers into i-Master PCR tube.
tube contains dNTP, buffer and chemical stabilizer. Two tube system Note : Recommended volume of template and primer : 3㎕~5㎕
improves the stability and activity of i-TaqTM DNA polymerase. Appropriate amounts of template samples.
•cDNA : 0.5-10% of first RT reaction volume
STORAGE •Plasmid DNA : 10pg-100ng
Store at -20℃. i-Master mix solution should be stored at 4oC after receiving, •Genomic DNA : 0.2-2ug for single copy
and then stable for 6 months. If more then 6 months, must be eliquoted, and Note : Appropriate amounts of primers
then stored at –20℃. •Primer : 10-30uM each (sense and anti -sense)
2. Add i-Master mix Solution into the i-Master PCR tubes to a total volume
CHARACTERISTICS
of 20㎕.
-Ready to use with only template DNA and primers
Note : if you use 3? l of template DNA and primers, add 17㎕ i-Master
-Stable for over 1 year at -20 ℃ mix solution.
-Saving money and time
-Constructed as a reaction volume of 20㎕ 3. Dissolve the blue pellet by pipetting.
Note : If the mixture lets stand at RT for 1-2min after adding i-Master
KIT CONTENT mix solution, the pellet is easily dissolved.
•i-Master PCR tube 96 (500) tubes 4. (Option) Add mineral oil.
i-Master PCR tube (component)* Note : This step is unnecessary when using a thermal cycler that
employs a top heating method(general method).
i-Taq DNA Polymerase 2.5U
5. Perform PCR of samples.
Chemical stabilizer I 1X
6. Load samples on agarose gel without adding a loading-dye buffer and
Loading buffer 1X perform electrophoresis.
*Lyophilized under iNtRON’s instruction
SUGGESTED CYCLING PARAMETERS
•i-Master mix Solution 2 (9)ml
Note : The PCR process is covered by patents issued and applicable in certain
A countries. iNtRON Biotechnology does not encourage or support the unauthorized or
unlicensed use of the PCR process. Use of this product is recommended for persons
that either have a license to perform PCR or are not required to obtain a license.
B
iNtRON BIOTECHNOLOGY
www.intronbio.com
info@intronbio.com