Professional Documents
Culture Documents
for
Coliform
Contamination
Fixing Positive Coliform Results
Fixing Positive Coliform Results
• Adopt the mentality that every positive
coliform result has a source.
• Thoroughly investigate to determine
potential sources.
• Do not flush and disinfect the entire
system following a positive routine
result.
Fixing Positive Coliform Results
• Understand that repeat samples are intended
to determine whether coliform
contamination is widespread or isolated.
• Take repeat samples at the correct locations.
• Review past monitoring results to see if
there are any trends.
Fixing Positive Coliform Results
• If the system uses sodium hypochlorite for
disinfection, review:
– procedures for replacing or freshening-up the hypochlorite
feed solution
– chlorine residual monitoring frequency in the distribution
system
– locations for chlorine residual monitoring
– expiration date of test kit chemicals
– water usage records, particularly peak usage vs. chlorine
feed and contact time
Investigative Sampling
• If after thoroughly inspecting the system for potential
pathways for the coliform contamination you still have
not identified a source, take investigative samples of
the:
– water source
– storage tank
– each section of the distribution system
• Check the box on the the bacteriological sampling
form that the samples are “special” or “investigative”.
If Potential Coliform Source
Cannot Be Identified
• Shutdown the system if a source cannot be
found.
• Flush and disinfect the storage tank and
distribution system completely if a source
cannot be identified.
• If the source is determined to be
contaminated, disinfect it obtaining a free
chlorine residual of 100 mg/L.
If Potential Coliform Source
Cannot Be Identified
• Premix your chlorine solution in a container.
• Pour the solution into the well through a hose
which the hose is being raised and lowered
through the water.
• Hold the chlorine in the source for at least 24-
hours.
• Pump the well or flush out the spring box until
the chlorine residual is 1 mg/l or less.
Disinfection of the Distribution
System
• To disinfect the distribution system chlorinate the
source and pump or feed the water into the system.
• Let the water run out of each tap until a chlorine
odor is detected.
• Hold the chlorinated water in the distribution
system for at least 24-hours.
• Keep the chlorinated water out of nearby surface
water.
• Start your search at the
well house.
Check the well casing to see if its
properly sealed
An incomplete repair may be a
source
Can surface water runoff
infiltrate around the well casing?
• Seal any cracks in the
concrete pad around the
casing.
• Pad should extend above
grade and slope away
from the casing.
• Hand pump pad should
have a drain that
discharges to grade at
least 100 feet away.
Surface water runoff into vaults associated
with the water system is another potential
pathway for contaminates
Surface water runoff and
sediment has potentially
contaminated the filter