TRIPLE SUGAR IRON

TEST
TRIPLE SUGAR IRON TEST
 To detect the ability of microbes to ferment sugar.

 TSI slant contain

Agar
PH sensitive dye (phenol red)
1% lactose
1%sucrose
0.1% Glucose
Sodium thiosulfate
Ferrous sulfate
 TSI reaction for differentiation of Enteric Organisms

Alkaline slant/Alkaline or Acid slant, Acid butt, No Acid slant ,Acid butt, H2S Alkaline Butt. Acid butt, no Alkaline slant ,Acid butt,
no change butt H2S production H2S no H2S

Alkaligenic Pseudomonas
Actinobacter
Escherichia Klebsiella
Enterobactor
Citrobactor Anizone Shigella Salmonella Arizona
Citrobactor