ASEPTIC BEHAVIOUR

UNIVERSITAS PANCASILA
CLEANROOM BEHAVIOUR
“Poor cGMP conditions…can ultimately pose a life threatening health
risk to a patient”

Even successfully qualified systems can be compromised by:

®poor personnel activities
®poor operational activities
®poor maintenance activities
CLEANROOM BEHAVIOUR
POOR Personal Activity

BEHAVIOUR IN CLEANROOM

Any persons who pose an unacceptable risk to the product,

process or facility must be excluded immediately and retrained
ASEPTIC BEHAVIOUR
Everyone entering
cleanrooms/zones must:
• be trained and competent, or
• must be ‘closely’ supervised
Everyone has a responsibility to:
• be familiar/compliant with
expected standards
• report substandard behaviour (e.g. critical activity, product, test, or
gowning)
• report damaged room fabric,
equipment, gowns
Clean zone : an area with a
defined particulate and
microbiological cleanliness
standard, usually consisting of
Laminar Air Flow protection
A clean zone is designed to
maintain asepsis or sterility of the
material contained within it
CLEANROOM BEHAVIOUR
Tidak memiliki kuku yang panjang.
Do not have long nails.
Tidak berkumis, berjanggut atau bercambang.
No grow mustache, bearded or sideburn.
Tidak memakai perhiasan, jam tangan dan kosmetik di
area ruang bersih.
Do not wear jewelry, watches and cosmetics in the
clean room area.
Mencuci tangan sebelum masuk ke area kerja.
Wash hands before entering work area.
Tidak merokok, makan dan minum di area kerja kecuali
di area yang memang diperuntukkan untuk kegiatan
tersebut.
Don't smoke, eat and drink in the work area except in
areas that are intended for these activities
Personil yang mengidap penyakit atau menderita luka terbuka sebaiknya
tidak dilibatkan dalam kegiatan di area ruang bersih.
Personnel who shows apparent illness or open lesion should not be
involved in activities in the clean room area.
Patuhi semua prosedur alur keluar masuk ruang bersih pada masing-
masing gedung.
Follow all procedures to enter and exit of the cleanroom in each
building.
Hanya personil yang telah terkualifikasi yang boleh memasuki ruang
bersih.
Only qualified personnel may enter the clean room.
Dilarang membawa barang yang menghasilkan partikel kedalam ruang
bersih.
Do not carry items that produce particles into the clean room.
Hanya barang yang telah disterilisasi atau disanitasi yang dapat masuk
kedalam ruang bersih.
Only items that have been sterilized or sanitized can enter the clean
room.
Personil tidak boleh bersandar.
Personnel shall not be leaning
CLEANROOM BEHAVIOUR
Segera ganti sarung tangan bila sobek atau tercemar.
Immediately change the gloves whenever it torn or spoiled.
Semua rambut harus tertutup secara menyeluruh setiap saat.
All hair shall be covered thoroughly all the time.
Ritsleting pakaian terusan tidak boleh dibuka pada area ruang
bersih.
The zipper of coverall shall not be opened in cleanroom area.
Tidak boleh ada bagian kulit di antara sarung tangan dan pakaian
terusan yang terpapar.
Any exposed skin between the gloves and coverall is prohibited.
Saat sudah berada di area ruang bersih, personil yang bersangkutan
harus sedapat mungkin mencegah dirinya kembali ke ruang
penyangga udara. Bila personil harus pergi ke toilet, prosedur
pergantian pakaian harus dilakukan sebelum memasuki kembali
area ruang bersih.
When already in cleanroom area, repective personnel shall minimize
going to airlock. In case personnel must go to the toilet, procedure
of changing the gowns must be performed before going back to
cleanroom area
Baju ruang bersih yang telah rusak, sobek, kotor atau belum
disterilisasi tidak boleh digunakan, apabila rusak atau sobek selama
melakukan kegiatan operasional, personil yang bersangkutan harus
segara mengganti baju di ruang ganti pakaian.
Clean room gowns that have been damaged, torn, dirty or not
sterilized should not be used, in case any damaged or torn during
operational activity, respective personnel must be immediately
changing the gowns in the changing room.
Hindarkan gerakan cepat yang tidak perlu dan berjalan tergesa-gesa
atau berlari.
Avoid unnecessary fast motion and walking hastily or running.
Dilarang berteriak, tertawa, bersiul, bernyanyi, bercanda dan
berbicara yang tidak perlu karena akan menambah jumlah bakteri
yang keluar dari mulut.
Do not screaming, laughing, whistling, singing, joking, and do
unnecessary talking since it increases bacterial number from
mouth.
Hindarkan menggaruk kepala atau menggosok tangan, muka, atau
bagian tubuh lain.
Avoid scratching head or rubbing hand, face, or other parts of body
CLEANROOM BEHAVIOUR
Minimalkan jumlah orang didalam area kelas bersih karena manusia
adalah sumber kontaminasi terbesar.
Minimize the number of people in the clean class area because
humans are the biggest source of contamination.
Batas waktu bekerja didalam area ruang bersih hanya 4 jam. Ganti
semua perlengkapan baru setelah melewati batas 4 jam.
The deadline for working in a clean room area is only 4 hours.
Replace all new equipment after passing the 4 hour limit.
Baju yang telah digunakan tidak boleh digunakan kembali sebelum
dicuci dan disterilisasi.
The gowns that have been used should not be reused before
washing and sterilizing.
Semprot sarung tangan dengan larutan desinfektan segera setelah
memegang atau bersentuhan dengan bagian dari instrument atau
benda lain.
Spray gloves with disinfectant immediately after holding or
touching part of instrument or other things.
Gunakan teknik aspetik jika dipersyaratkan
Aseptic techiniques must always be used if required
Baju ruang bersih yang telah rusak, sobek, kotor atau belum
disterilisasi tidak boleh digunakan, apabila rusak atau sobek selama
melakukan kegiatan operasional, personil yang bersangkutan harus
segara mengganti baju di ruang ganti pakaian.
Clean room gowns that have been damaged, torn, dirty or not
sterilized should not be used, in case any damaged or torn during
operational activity, respective personnel must be immediately
changing the gowns in the changing room.
Hindarkan gerakan cepat yang tidak perlu dan berjalan tergesa-gesa
atau berlari.
Avoid unnecessary fast motion and walking hastily or running.
Dilarang berteriak, tertawa, bersiul, bernyanyi, bercanda dan
berbicara yang tidak perlu karena akan menambah jumlah bakteri
yang keluar dari mulut.
Do not screaming, laughing, whistling, singing, joking, and do
unnecessary talking since it increases bacterial number from
mouth.
Hindarkan menggaruk kepala atau menggosok tangan, muka, atau
bagian tubuh lain.
Avoid scratching head or rubbing hand, face, or other parts of body
CLEANROOM BEHAVIOUR
• ASEPTIC TECHNIQUE PRINCIPLE
Fundamental and important laboratory skill in the field of microbiology.
Aseptic technique use for a variety of procedures such as transferring cultures, inoculating media, isolation of
pure cultures, and for performing microbiological tests.
Proper aseptic technique prevents contamination of cultures from foreign bacteria inherent in the
environment. For example, airborne microorganisms (including fungi), microbes picked up from the
researcher’s body, the lab bench-top or other surfaces, microbes found in dust, as well as microbes found on
unsterilized glassware and equipment, etc. may potentially contaminate cultures, thus interfering with the lab
results.
Proper aseptic technique can greatly minimize or even eliminate the risk of contamination. In addition, aseptic
technique is of utmost importance to maintain pure stock cultures while transferring cultures to new media.
Aseptic technique is also essential for isolation of a single species of microorganism from a mixed culture to
obtain a pure culture. Furthermore, proper aseptic technique prevents microbes used in the laboratory from
accidentally being released into the environment and/ or infecting people working in the laboratory. This is
especially relevant when pathogens are being handled.
CLEANROOM BEHAVIOUR
• ASEPTIC TECHNIQUE PRINCIPLE
Flaming :
• Flaming the loop :  Holding the loop in the flame of the Bunsen burner kills all contaminating organisms, thus sterilizing
the loop.   The loop should glow red-hot for a few seconds. After flaming, make sure to slightly cool the loop before
picking up organisms from the inoculum culture (the culture that is to be transferred.) 
When transferring a culture from a plate, cool the loop by touching on the very edge of agar.  When transferring from a
broth, the red-hot loop will make a sizzling noise as soon as   you insert it into the culture.  The loop will automatically
cool once it makes contact with the broth culture, but wait a one or two seconds before removing the loop full of
inoculum from the tube. (The hot loop may create aerosols when it touches the media containing microorganisms. It will
cause some of the broth and bacteria to boil briefly, creating a bacteria-containing aerosol. This airborne bacteria have
the chances of entering into the respiratory tract or into the body parts.
If you hear a hissing sound when you place the heat sterilized loop into the broth culture indicates that the loop is not
cooled sufficiently).
• Flaming the Mouth of the Test Tube:  Passing the mouth of a tube through the flame of a Bunsen burner creates a
convection current which forces air out of the tube.  This prevents airborne contaminants from entering the tube. The
heat of the Bunsen burner also causes the air around your work area to rise, reducing the chance of airborne
microorganisms contaminating your cultures. 
TERIMAKASIH